Restriction map for
pFN21ASDF0070
(Circular) (Six-base) MAPSORT of: pFN21ASDF0070.seq Check: 4753 from: 1 to: 8635
pFN21ASDF0070 8635 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2802 2802
Size: 8635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6445 278
Size: 53 83 186 936 4909 2468
Fragments arranged by size:
4909 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4902 5978 1937
Size: 2965 1076 4594
Fragments arranged by size:
4594 2965 1076
Acc65I G'GTAC_C
Cuts at: 2468 5956 2468
Size: 3488 5147
AclI AA'CG_TT
Cuts at: 1563 6762 7135 1563
Size: 5199 373 3063
Fragments arranged by size:
5199 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4661 6698 8082 8350 1632
Size: 3029 2037 1384 268 1917
Fragments arranged by size:
3029 2037 1917 1384 268
AfeI AGC'GCT
Cuts at: 2201 3344 5834 2201
Size: 1143 2490 5002
Fragments arranged by size:
5002 2490 1143
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8438
AflIII A'CryG_T
Cuts at: 1203 4398 5427 7534 8511 1203
Size: 3195 1029 2107 977 1327
Fragments arranged by size:
3195 2107 1327 1029 977
AgeI A'CCGG_T
Cuts at: 3568 3834 7466 3568
Size: 266 3632 4737
Fragments arranged by size:
4737 3632 266
AhdI GACnn_n'nnGTC
Cuts at: 7364 7364
Size: 8635
AleI CACnn'nnGTG
Cuts at: 1166 2996 4737 4826 1166
Size: 1830 1741 89 4975
Fragments arranged by size:
4975 1830 1741 89
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8635
AlwNI CAG_nnn'CTG
Cuts at: 2407 2794 3014 3743 4278 4929 5245 6378 7950
Size: 387 220 729 535 651 316 1133 1572
Cuts at: 7950 2407
Size: 3092
Fragments arranged by size:
3092 1572 1133 729 651 535 387 316
220
ApaI G_GGCC'C
Cuts at: 2034 2509 2610 3800 5367 2034
Size: 475 101 1190 1567 5302
Fragments arranged by size:
5302 1567 1190 475 101
ApaLI G'TGCA_C
Cuts at: 2744 6692 7848 2744
Size: 3948 1156 3531
Fragments arranged by size:
3948 3531 1156
ApoI r'AATT_y
Cuts at: 1493 5944 6170 6322 8431 1493
Size: 4451 226 152 2109 1697
Fragments arranged by size:
4451 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7189 160
Size: 872 6157 1606
Fragments arranged by size:
6157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8635
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 2034 2609 2659 2726 3638 3898
Size: 73 84 7 575 50 67 912 260
Cuts at: 3898 4184 4306 4639 5360 5390 5960 1870
Size: 286 122 333 721 30 570 4545
Fragments arranged by size:
4545 912 721 575 570 333 286 260
122 84 73 67 50 30 7
AvrII C'CTAG_G
Cuts at: 2653 2653
Size: 8635
BamHI G'GATC_C
Cuts at: 5965 6332 5965
Size: 367 8268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2443 2468 2647 3359 3974
Size: 325 217 627 656 25 179 712 615
Cuts at: 3974 5356 5531 5720 5956 7411 618
Size: 1382 175 189 236 1455 1842
Fragments arranged by size:
1842 1455 1382 712 656 627 615 325
236 217 189 179 175 25
BanII G_rGCy'C
Cuts at: 1391 2034 2282 2336 2509 2610 2762 3251 3509
Size: 643 248 54 173 101 152 489 258
Cuts at: 3509 3800 3891 4313 4685 5252 5327 5367 5399
Size: 291 91 422 372 567 75 40 32
Cuts at: 5399 5954 1391
Size: 555 4072
Fragments arranged by size:
4072 643 567 555 489 422 372 291
258 248 173 152 101 91 75 54
40 32
BbeI G_GCGC'C
Cuts at: 3978 5535 5724 3978
Size: 1557 189 6889
Fragments arranged by size:
6889 1557 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2928 4131 928
Size: 971 1029 1203 5432
Fragments arranged by size:
5432 1203 1029 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2385 2419 4999 5033 6826 6860 1903
Size: 34 448 34 2580 34 1793 34 3678
Fragments arranged by size:
3678 2580 1793 448 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3392 6527 7737 3392
Size: 3135 1210 4290
Fragments arranged by size:
4290 3135 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2133 3168 4164
Size: 107 122 71 631 605 461 1035 996
Cuts at: 4164 4233 4458 5301 5517 7246 136
Size: 69 225 843 216 1729 1525
Fragments arranged by size:
1729 1525 1035 996 843 631 605 461
225 216 122 107 71 69
BglII A'GATC_T
Cuts at: 2667 3378 8630 2667
Size: 711 5252 2672
Fragments arranged by size:
5252 2672 711
BlpI GC'TnA_GC
Cuts at: 4909 6052 4909
Size: 1143 7492
Bme1580I G_kGCm'C
Cuts at: 1790 2034 2360 2448 2509 2610 2652 2748 3800
Size: 244 326 88 61 101 42 96 1052
Cuts at: 3800 4333 5361 5367 5413 5552 6696 7852 1790
Size: 533 1028 6 46 139 1144 1156 2573
Fragments arranged by size:
2573 1156 1144 1052 1028 533 326 244
139 101 96 88 61 46 42 6
BmgBI CAC'GTC
Cuts at: 1318 4252 4399 5128 5266 5428 1318
Size: 2934 147 729 138 162 4525
Fragments arranged by size:
4525 2934 729 162 147 138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2436 4892 6366
Size: 416 53 83 399 24 1019 2456 1474
Cuts at: 6366 7324 442
Size: 958 1753
Fragments arranged by size:
2456 1753 1474 1019 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8635
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 3664 2566
Size: 1098 7537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5455 7295 1239
Size: 4216 1840 2579
Fragments arranged by size:
4216 2579 1840
Bpu10I CC'TnA_GC
Cuts at: 2479 2492 3252 5246 2479
Size: 13 760 1994 5868
Fragments arranged by size:
5868 1994 760 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 6760 7625 7923 2231
Size: 4529 865 298 2943
Fragments arranged by size:
4529 2943 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7298 882
Size: 617 5799 2219
Fragments arranged by size:
5799 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3020 3970 5932 6426 493
Size: 711 1816 950 1962 494 2702
Fragments arranged by size:
2702 1962 1816 950 711 494
BsaBI GATnn'nnATC
Cuts at: 8629 8629
Size: 8635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3975 5532 5721 5757
Size: 53 83 186 936 2442 1557 189 36
Cuts at: 5757 6442 6824 7459 275
Size: 685 382 635 1451
Fragments arranged by size:
2442 1557 1451 936 685 635 382 189
186 83 53 36
BsaWI w'CCGG_w
Cuts at: 2539 3214 3499 3568 3834 4479 7068 7466 7740
Size: 675 285 69 266 645 2589 398 274
Cuts at: 7740 7887 8298 2539
Size: 147 411 2876
Fragments arranged by size:
2876 2589 675 645 411 398 285 274
266 147 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3128 3158 3604 3634 3774
Size: 30 165 30 1725 30 446 30 140
Cuts at: 3774 3804 5030 5060 1178
Size: 30 1226 30 4753
Fragments arranged by size:
4753 1725 1226 446 165 140 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2380 2469 3233 3593 4757 5678 1186
Size: 1194 89 764 360 1164 921 4143
Fragments arranged by size:
4143 1194 1164 921 764 360 89
BseYI C'CCAG_C
Cuts at: 2122 2628 2787 2979 3385 3389 3489 3846 4391
Size: 506 159 192 406 4 100 357 545
Cuts at: 4391 4833 4866 4984 5526 5916 7838 8334 2122
Size: 442 33 118 542 390 1922 496 2423
Fragments arranged by size:
2423 1922 545 542 506 496 442 406
390 357 192 159 118 100 33 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2702 3020 3449 3611 3950 4306 4648
Size: 573 966 318 429 162 339 356 342
Cuts at: 4648 5188 1163
Size: 540 4610
Fragments arranged by size:
4610 966 573 540 429 356 342 339
318 162
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2157 2828 4360 5374 5403 5546
Size: 275 186 156 671 1532 1014 29 143
Cuts at: 5546 5714 6085 6846 7874 1540
Size: 168 371 761 1028 2301
Fragments arranged by size:
2301 1532 1028 1014 761 671 371 275
186 168 156 143 29
BsiHKAI G_wGCw'C
Cuts at: 2390 2748 2762 3258 6696 6781 7852 2390
Size: 358 14 496 3438 85 1071 3173
Fragments arranged by size:
3438 3173 1071 496 358 85 14
BsmI GAATG_Cn'
Cuts at: 4013 5123 6146 6239 4013
Size: 1110 1023 93 6409
Fragments arranged by size:
6409 1110 1023 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2282 2336 2360 2390 2448 2509
Size: 399 244 248 54 24 30 58 61
Cuts at: 2509 2610 2652 2748 2762 3251 3258 3509 3800
Size: 101 42 96 14 489 7 251 291
Cuts at: 3800 3891 4313 4333 4685 5252 5327 5361 5367
Size: 91 422 20 352 567 75 34 6
Cuts at: 5367 5399 5413 5552 5954 6696 6781 7852 1391
Size: 32 14 139 402 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 489 422 402 399
352 291 251 248 244 139 101 96
91 85 75 61 58 54 42 34
32 30 24 20 14 14 7 6
BspEI T'CCGG_A
Cuts at: 2539 3499 8298 2539
Size: 960 4799 2876
Fragments arranged by size:
4799 2876 960
BspHI T'CATG_A
Cuts at: 6524 8254 6524
Size: 1730 6905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2802 5118 5325 5990 844
Size: 1958 2316 207 665 3489
Fragments arranged by size:
3489 2316 1958 665 207
BsrBI CCG'CTC
Cuts at: 1394 1823 2164 5419 6522 1394
Size: 429 341 3255 1103 3507
Fragments arranged by size:
3507 3255 1103 429 341
BsrDI GCAATG_nn'
Cuts at: 65 1248 3105 3201 5572 7130 7304 65
Size: 1183 1857 96 2371 1558 174 1396
Fragments arranged by size:
2371 1857 1558 1396 1183 174 96
BsrFI r'CCGG_y
Cuts at: 1700 1810 2152 2156 2221 2459 2471 3568 3834
Size: 110 342 4 65 238 12 1097 266
Cuts at: 3834 3923 4092 4862 5876 7279 7466 1700
Size: 89 169 770 1014 1403 187 2869
Fragments arranged by size:
2869 1403 1097 1014 770 342 266 238
187 169 110 89 65 12 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8635
BssHII G'CGCG_C
Cuts at: 1925 3330 5885 1925
Size: 1405 2555 4675
Fragments arranged by size:
4675 2555 1405
BssSI C'ACGA_G
Cuts at: 3988 6695 7707 3988
Size: 2707 1012 4916
Fragments arranged by size:
4916 2707 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2797 3075 4278 4608 794
Size: 2003 278 1203 330 4821
Fragments arranged by size:
4821 2003 1203 330 278
BstBI TT'CG_AA
Cuts at: 2007 7439 2007
Size: 5432 3203
BstEII G'GTnAC_C
Cuts at: 1184 3553 1184
Size: 2369 6266
BstYI r'GATC_y
Cuts at: 1972 2667 3378 4433 5965 6332 6717 6734 8186
Size: 695 711 1055 1532 367 385 17 1452
Cuts at: 8186 8272 8284 8630 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1532 1452 1055 711 695 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3528 3528
Size: 8635
BtgI C'CryG_G
Cuts at: 513 1065 2697 3118 3558 3778 3952 4657 5521
Size: 552 1632 421 440 220 174 705 864
Cuts at: 5521 5708 513
Size: 187 3440
Fragments arranged by size:
3440 1632 864 705 552 440 421 220
187 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4186 4716 5568 487
Size: 683 56 2960 530 852 3554
Fragments arranged by size:
3554 2960 852 683 530 56
BtsI GCAGTG_nn'
Cuts at: 2943 3073 3316 3817 3943 4486 4876 6151 6945
Size: 130 243 501 126 543 390 1275 794
Cuts at: 6945 6965 2943
Size: 20 4613
Fragments arranged by size:
4613 1275 794 543 501 390 243 130
126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8600
DraI TTT'AAA
Cuts at: 5939 5939
Size: 8635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7642 809
Size: 507 6326 1802
Fragments arranged by size:
6326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 2308 2380
Size: 54 1425 50 275 18 324 154 72
Cuts at: 2380 2556 2825 3169 3775 4357 4818 5302 5371
Size: 176 269 344 606 582 461 484 69
Cuts at: 5371 5400 5475 5695 5711 6082 6971 8600 8
Size: 29 75 220 16 371 889 1629 43
Fragments arranged by size:
1629 1425 889 606 582 484 461 371
344 324 275 269 220 176 154 75
72 69 54 50 43 29 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2154 2825 4357 5371 5400 5711 6082
Size: 275 342 671 1532 1014 29 311 371
Cuts at: 6082 1537
Size: 4090
Fragments arranged by size:
4090 1532 1014 671 371 342 311 275
29
EarI CTCTTCn'nnn_
Cuts at: 1353 3218 3537 5250 6565 1353
Size: 1865 319 1713 1315 3423
Fragments arranged by size:
3423 1865 1713 1315 319
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3227 7222 7596 7742 387
Size: 1064 138 1638 3995 374 146 1280
Fragments arranged by size:
3995 1638 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2760 2760
Size: 8635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4661 5455 6698 7295 8082 8350 1239
Size: 393 3029 794 1243 597 787 268 1524
Fragments arranged by size:
3029 1524 1243 794 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2031 2483 2505 2506 2618 2686 3011 3182 3797
Size: 452 22 1 112 68 325 171 615
Cuts at: 3797 4144 4239 4636 4662 5281 5364 5568 5904
Size: 347 95 397 26 619 83 204 336
Cuts at: 5904 2031
Size: 4762
Fragments arranged by size:
4762 619 615 452 397 347 336 325
204 171 112 95 83 68 26 22
1
EcoRI G'AATT_C
Cuts at: 5944 6322 5944
Size: 378 8257
EcoRV GAT'ATC
Cuts at: 5782 5782
Size: 8635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2230 5107 2230
Size: 2877 5758
FseI GG_CCGG'CC
Cuts at: 2156 2156
Size: 8635
FspI TGC'GCA
Cuts at: 3097 7141 3097
Size: 4044 4591
HaeII r_GCGC'y
Cuts at: 1678 2099 2189 2203 2434 2525 3036 3346 3711
Size: 421 90 14 231 91 511 310 365
Cuts at: 3711 3978 3988 5239 5535 5724 5836 5923 7782
Size: 267 10 1251 296 189 112 87 1859
Cuts at: 7782 1678
Size: 2531
Fragments arranged by size:
2531 1859 1251 511 421 365 310 296
267 231 189 112 91 90 87 14
10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3569 3601 3637 3669 4136
Size: 32 135 32 1997 32 36 32 467
Cuts at: 4136 4168 7276 7308 7350 7382 1373
Size: 32 3108 32 42 32 2626
Fragments arranged by size:
3108 2626 1997 467 135 42 36 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4972 5314 5979 6225 669
Size: 988 281 3034 342 665 246 3079
Fragments arranged by size:
3079 3034 988 665 342 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8635
HpaI GTT'AAC
Cuts at: 6225 6225
Size: 8635
KasI G'GCGC_C
Cuts at: 3974 5531 5720 3974
Size: 1557 189 6889
Fragments arranged by size:
6889 1557 189
KpnI G_GTAC'C
Cuts at: 2472 5960 2472
Size: 3488 5147
MfeI C'AATT_G
Cuts at: 3937 6234 3937
Size: 2297 6338
MluI A'CGCG_T
Cuts at: 8511 8511
Size: 8635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2307 5004 5175 7749 7933 1305
Size: 1002 2697 171 2574 184 2007
Fragments arranged by size:
2697 2574 2007 1002 184 171
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2558 3171 3777 4820 10
Size: 54 1425 343 726 613 606 1043 3825
Fragments arranged by size:
3825 1425 1043 726 613 606 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2996 3914 4737 4826 6593 6952 7111
Size: 648 1830 918 823 89 1767 359 159
Cuts at: 7111 518
Size: 2042
Fragments arranged by size:
2042 1830 1767 918 823 648 359 159
89
MspA1I CmG'CkG
Cuts at: 1606 2853 2983 3365 3389 3954 4320 5196 5307
Size: 1247 130 382 24 565 366 876 111
Cuts at: 5307 5334 5523 5700 5710 5772 5850 5862 6051
Size: 27 189 177 10 62 78 12 189
Cuts at: 6051 6728 7876 8121 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1247 1148 876 677 565 382 366
245 189 189 177 130 111 78 62
27 24 12 10
NaeI GCC'GGC
Cuts at: 2154 2158 4864 5878 2154
Size: 4 2706 1014 4911
Fragments arranged by size:
4911 2706 1014 4
NarI GG'CG_CC
Cuts at: 3975 5532 5721 3975
Size: 1557 189 6889
Fragments arranged by size:
6889 1557 189
NcoI C'CATG_G
Cuts at: 513 1065 3118 4657 513
Size: 552 2053 1539 4491
Fragments arranged by size:
4491 2053 1539 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8635
NgoMIV G'CCGG_C
Cuts at: 2152 2156 4862 5876 2152
Size: 4 2706 1014 4911
Fragments arranged by size:
4911 2706 1014 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8635
NotI GC'GGCC_GC
Cuts at: 6082 6082
Size: 8635
NruI TCG'CGA
Cuts at: 2395 4248 2395
Size: 1853 6782
NspI r_CATG'y
Cuts at: 1132 1229 5014 5461 5993 7538 1132
Size: 97 3785 447 532 1545 2229
Fragments arranged by size:
3785 2229 1545 532 447 97
PasI CC'CwG_GG
Cuts at: 1348 2845 3046 4333 4579 5352 1348
Size: 1497 201 1287 246 773 4631
Fragments arranged by size:
4631 1497 1287 773 246 201
PciI A'CATG_T
Cuts at: 7534 7534
Size: 8635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3356 3675 1108
Size: 2248 319 6068
Fragments arranged by size:
6068 2248 319
PmeI GTTT'AAAC
Cuts at: 5939 5939
Size: 8635
PmlI CAC'GTG
Cuts at: 3970 3970
Size: 8635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6704 6736 8217 8249 6704
Size: 32 1481 32 7090
Fragments arranged by size:
7090 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4144 4636 5281 5568 4144
Size: 492 645 287 7211
Fragments arranged by size:
7211 645 492 287
PshAI GACnn'nnGTC
Cuts at: 1613 2268 5679 1613
Size: 655 3411 4569
Fragments arranged by size:
4569 3411 655
PsiI TTA'TAA
Cuts at: 6205 6205
Size: 8635
PspOMI G'GGCC_C
Cuts at: 2030 2505 2606 3796 5363 2030
Size: 475 101 1190 1567 5302
Fragments arranged by size:
5302 1567 1190 475 101
PstI C_TGCA'G
Cuts at: 830 2988 3311 4421 4522 5987 830
Size: 2158 323 1110 101 1465 3478
Fragments arranged by size:
3478 2158 1465 1110 323 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8635
PvuII CAG'CTG
Cuts at: 2983 3389 2983
Size: 406 8229
SacI G_AGCT'C
Cuts at: 2762 2762
Size: 8635
SacII CC_GC'GG
Cuts at: 3955 5524 5711 3955
Size: 1569 187 6879
Fragments arranged by size:
6879 1569 187
SalI G'TCGA_C
Cuts at: 1936 5977 1936
Size: 4041 4594
SanDI GG'GwC_CC
Cuts at: 4144 5568 4144
Size: 1424 7211
SapI GCTCTTCn'nnn_
Cuts at: 3218 3218
Size: 8635
SbfI CC_TGCA'GG
Cuts at: 5987 5987
Size: 8635
ScaI AGT'ACT
Cuts at: 6883 8595 6883
Size: 1712 6923
SfcI C'TryA_G
Cuts at: 826 1045 2984 3307 4417 4518 4561 4903 5983
Size: 219 1939 323 1110 101 43 342 1080
Cuts at: 5983 7118 7799 7990 826
Size: 1135 681 191 1471
Fragments arranged by size:
1939 1471 1135 1110 1080 681 342 323
219 191 101 43
SfoI GGC'GCC
Cuts at: 3976 5533 5722 3976
Size: 1557 189 6889
Fragments arranged by size:
6889 1557 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8635
SgrAI Cr'CCGG_yG
Cuts at: 1700 2221 3568 3923 1700
Size: 521 1347 355 6412
Fragments arranged by size:
6412 1347 521 355
SmaI CCC'GGG
Cuts at: 1872 2029 2611 2661 3900 4186 4308 5362 5962
Size: 157 582 50 1239 286 122 1054 600
Cuts at: 5962 1872
Size: 4545
Fragments arranged by size:
4545 1239 1054 600 582 286 157 122
50
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2246 6739 7640 7902 820
Size: 197 926 303 4493 901 262 1553
Fragments arranged by size:
4493 1553 926 901 303 262 197
SnaBI TAC'GTA
Cuts at: 493 5932 6426 493
Size: 5439 494 2702
Fragments arranged by size:
5439 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8635
SphI G_CATG'C
Cuts at: 1132 5993 1132
Size: 4861 3774
SrfI GCCC'GGGC
Cuts at: 2029 5362 2029
Size: 3333 5302
SspI AAT'ATT
Cuts at: 5 52 6559 5
Size: 47 6507 2081
Fragments arranged by size:
6507 2081 47
StuI AGG'CCT
Cuts at: 1517 2732 3323 1517
Size: 1215 591 6829
Fragments arranged by size:
6829 1215 591
StyI C'CwwG_G
Cuts at: 513 1065 2653 3118 4657 6074 513
Size: 552 1588 465 1539 1417 3074
Fragments arranged by size:
3074 1588 1539 1417 552 465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2849 3086 3285 3496 3618 4586 4736
Size: 1255 1269 237 199 211 122 968 150
Cuts at: 4736 4967 5344 6662 6679 6832 7017 7462 325
Size: 231 377 1318 17 153 185 445 1498
Fragments arranged by size:
1498 1318 1269 1255 968 445 377 237
231 211 199 185 153 150 122 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2988 5220 6881
Size: 274 80 33 51 1443 1011 2232 1661
Cuts at: 6881 8593 96
Size: 1712 138
Fragments arranged by size:
2232 1712 1661 1443 1011 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3604 3636 3774 3806 5030 5062 5444 5476 8595
Size: 32 138 32 1224 32 382 32 3119
Cuts at: 8595 8627 3604
Size: 32 3612
Fragments arranged by size:
3612 3119 1224 382 138 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8635
XbaI T'CTAG_A
Cuts at: 5971 5971
Size: 8635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3770 3770
Size: 8635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8635
XmaI C'CCGG_G
Cuts at: 1870 2027 2609 2659 3898 4184 4306 5360 5960
Size: 157 582 50 1239 286 122 1054 600
Cuts at: 5960 1870
Size: 4545
Fragments arranged by size:
4545 1239 1054 600 582 286 157 122
50
XmnI GAAnn'nnTTC
Cuts at: 6764 6764
Size: 8635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6443 276
Size: 53 83 186 936 4909 2468
Fragments arranged by size:
4909 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstXI
BstZ17I DraIII EcoNI FspAI NsiI PacI PfoI PspXI
PsrI RsrII SexAI SfiI SwaI