(Circular) (Six-base) MAPSORT of: pFN21ASDF0044.seq Check: 1637 from: 1 to: 7696 pFN21ASDF0044 7696 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2164 2346 2803 3102 3233 3550 4039 2164 Size: 182 457 299 131 317 489 5821 Fragments arranged by size: 5821 489 457 317 299 182 131 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3132 5506 278 Size: 53 83 186 936 1596 2374 2468 Fragments arranged by size: 2468 2374 1596 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5039 1937 Size: 3102 4594 Acc65I G'GTAC_C Cuts at: 5017 5017 Size: 7696 AclI AA'CG_TT Cuts at: 1563 5823 6196 1563 Size: 4260 373 3063 Fragments arranged by size: 4260 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3589 5759 7143 7411 1632 Size: 1957 2170 1384 268 1917 Fragments arranged by size: 2170 1957 1917 1384 268 AfeI AGC'GCT Cuts at: 2684 2684 Size: 7696 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7499 AflIII A'CryG_T Cuts at: 1203 6595 7572 1203 Size: 5392 977 1327 Fragments arranged by size: 5392 1327 977 AgeI A'CCGG_T Cuts at: 6527 6527 Size: 7696 AhdI GACnn_n'nnGTC Cuts at: 6425 6425 Size: 7696 AleI CACnn'nnGTG Cuts at: 1166 2477 2663 3654 4184 1166 Size: 1311 186 991 530 4678 Fragments arranged by size: 4678 1311 991 530 186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3672 1184 Size: 2488 5208 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2171 2203 3611 3643 4154 4186 2171 Size: 32 1408 32 511 32 5681 Fragments arranged by size: 5681 1408 511 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2827 2863 2953 3517 3565 4768 5439 7011 2827 Size: 36 90 564 48 1203 671 1572 3512 Fragments arranged by size: 3512 1572 1203 671 564 90 48 36 ApaI G_GGCC'C Cuts at: 3040 3463 4706 3040 Size: 423 1243 6030 Fragments arranged by size: 6030 1243 423 ApaLI G'TGCA_C Cuts at: 5753 6909 5753 Size: 1156 6540 ApoI r'AATT_y Cuts at: 1493 4853 5005 5231 5383 7492 1493 Size: 3360 152 226 152 2109 1697 Fragments arranged by size: 3360 2109 1697 226 152 152 AseI AT'TA_AT Cuts at: 160 1032 6250 160 Size: 872 5218 1606 Fragments arranged by size: 5218 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7696 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2117 2193 2310 2426 3350 3389 Size: 73 126 48 76 117 116 924 39 Cuts at: 3389 4869 5021 1870 Size: 1480 152 4545 Fragments arranged by size: 4545 1480 924 152 126 117 116 76 73 48 39 BamHI G'GATC_C Cuts at: 5026 5393 5026 Size: 367 7329 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2197 2314 2735 2784 3255 Size: 325 217 627 410 117 421 49 471 Cuts at: 3255 4217 4690 4739 4750 4888 4899 5017 6472 Size: 962 473 49 11 138 11 118 1455 Cuts at: 6472 618 Size: 1842 Fragments arranged by size: 1842 1455 962 627 473 471 421 410 325 217 138 118 117 49 49 11 11 BanII G_rGCy'C Cuts at: 1391 2208 2427 2714 3040 3065 3221 3398 3463 Size: 817 219 287 326 25 156 177 65 Cuts at: 3463 3920 4275 4608 4706 5015 1391 Size: 457 355 333 98 309 4072 Fragments arranged by size: 4072 817 457 355 333 326 309 287 219 177 156 98 65 25 BbeI G_GCGC'C Cuts at: 4694 4754 4892 4694 Size: 60 138 7498 Fragments arranged by size: 7498 138 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6725 BbvCI CC'TCA_GC Cuts at: 2135 2135 Size: 7696 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2288 2322 4592 4626 5887 5921 1903 Size: 34 351 34 2270 34 1261 34 3678 Fragments arranged by size: 3678 2270 1261 351 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5588 6798 5588 Size: 1210 6486 BclI T'GATC_A Cuts at: 1548 1548 Size: 7696 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3472 4379 4748 Size: 107 122 71 631 605 1800 907 369 Cuts at: 4748 4897 6307 136 Size: 149 1410 1525 Fragments arranged by size: 1800 1525 1410 907 631 605 369 149 122 107 71 BglII A'GATC_T Cuts at: 7691 7691 Size: 7696 BlpI GC'TnA_GC Cuts at: 2679 4517 5113 2679 Size: 1838 596 5262 Fragments arranged by size: 5262 1838 596 Bme1580I G_kGCm'C Cuts at: 1790 2319 2521 2740 3040 3258 3390 3463 3645 Size: 529 202 219 300 218 132 73 182 Cuts at: 3645 3661 4220 4651 4706 4717 4902 5757 6913 Size: 16 559 431 55 11 185 855 1156 Cuts at: 6913 1790 Size: 2573 Fragments arranged by size: 2573 1156 855 559 529 431 300 219 218 202 185 182 132 73 55 16 11 BmgBI CAC'GTC Cuts at: 1318 2475 4561 1318 Size: 1157 2086 4453 Fragments arranged by size: 4453 2086 1157 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3923 4745 5427 Size: 416 53 83 399 24 2506 822 682 Cuts at: 5427 6385 442 Size: 958 1753 Fragments arranged by size: 2506 1753 958 822 682 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7696 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2206 2614 2206 Size: 408 7288 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2439 3083 3194 3317 3332 3446 3548 Size: 1088 112 644 111 123 15 114 102 Cuts at: 3548 3764 3783 3857 4055 4889 6356 1239 Size: 216 19 74 198 834 1467 2579 Fragments arranged by size: 2579 1467 1088 834 644 216 198 123 114 112 111 102 74 19 15 Bpu10I CC'TnA_GC Cuts at: 2135 2202 2387 3059 2135 Size: 67 185 672 6772 Fragments arranged by size: 6772 672 185 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5821 6686 6984 5821 Size: 865 298 6533 Fragments arranged by size: 6533 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3833 3882 4868 6359 882 Size: 617 2334 49 986 1491 2219 Fragments arranged by size: 2334 2219 1491 986 617 49 BsaAI yAC'GTr Cuts at: 493 1204 2524 3379 4993 5487 493 Size: 711 1320 855 1614 494 2702 Fragments arranged by size: 2702 1614 1320 855 711 494 BsaBI GATnn'nnATC Cuts at: 4151 7690 4151 Size: 3539 4157 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2772 3129 3972 4691 Size: 53 83 186 936 1239 357 843 719 Cuts at: 4691 4751 4889 5503 5885 6520 275 Size: 60 138 614 382 635 1451 Fragments arranged by size: 1451 1239 936 843 719 635 614 382 357 186 138 83 60 53 BsaWI w'CCGG_w Cuts at: 6129 6527 6801 6948 7359 6129 Size: 398 274 147 411 6466 Fragments arranged by size: 6466 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2171 2201 2429 2459 3461 Size: 30 165 30 768 30 228 30 1002 Cuts at: 3461 3491 3524 3554 3611 3641 1178 Size: 30 33 30 57 30 5233 Fragments arranged by size: 5233 1002 768 228 165 57 33 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2195 2810 3329 3332 3521 3545 4283 Size: 901 108 615 519 3 189 24 738 Cuts at: 4283 1186 Size: 4599 Fragments arranged by size: 4599 901 738 615 519 189 108 24 3 BseYI C'CCAG_C Cuts at: 2160 2275 2707 3031 3170 3439 3445 3776 4021 Size: 115 432 324 139 269 6 331 245 Cuts at: 4021 4039 6899 7395 2160 Size: 18 2860 496 2461 Fragments arranged by size: 2860 2461 496 432 331 324 269 245 139 115 18 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 2654 3578 3887 1163 Size: 573 582 336 924 309 4972 Fragments arranged by size: 4972 924 582 573 336 309 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3879 4413 4747 5146 5907 6935 Size: 275 186 1878 534 334 399 761 1028 Cuts at: 6935 1540 Size: 2301 Fragments arranged by size: 2301 1878 1028 761 534 399 334 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 4275 4464 4608 5757 5842 6913 2304 Size: 1971 189 144 1149 85 1071 3087 Fragments arranged by size: 3087 1971 1149 1071 189 144 85 BsmI GAATG_Cn' Cuts at: 2547 2907 5207 5300 2547 Size: 360 2300 93 4943 Fragments arranged by size: 4943 2300 360 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2585 2585 Size: 7696 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2304 2319 2427 2521 2714 2740 Size: 399 418 96 15 108 94 193 26 Cuts at: 2740 3040 3065 3221 3258 3390 3398 3463 3645 Size: 300 25 156 37 132 8 65 182 Cuts at: 3645 3661 3920 4220 4275 4464 4608 4651 4706 Size: 16 259 300 55 189 144 43 55 Cuts at: 4706 4717 4902 5015 5757 5842 6913 1391 Size: 11 185 113 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 418 399 300 300 259 193 189 185 182 156 144 132 113 108 96 94 85 65 55 55 43 37 26 25 16 15 11 8 BspEI T'CCGG_A Cuts at: 7359 7359 Size: 7696 BspHI T'CATG_A Cuts at: 2937 3688 3795 5585 7315 2937 Size: 751 107 1790 1730 3318 Fragments arranged by size: 3318 1790 1730 751 107 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2164 2346 2803 3102 3233 3550 4039 5051 Size: 1320 182 457 299 131 317 489 1012 Cuts at: 5051 844 Size: 3489 Fragments arranged by size: 3489 1320 1012 489 457 317 299 182 131 BsrBI CCG'CTC Cuts at: 1394 1823 2185 2349 2391 3550 4477 4756 5583 Size: 429 362 164 42 1159 927 279 827 Cuts at: 5583 1394 Size: 3507 Fragments arranged by size: 3507 1159 927 827 429 362 279 164 42 BsrDI GCAATG_nn' Cuts at: 65 1248 6191 6365 65 Size: 1183 4943 174 1396 Fragments arranged by size: 4943 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2112 2753 3012 4412 4596 6340 6527 Size: 110 302 641 259 1400 184 1744 187 Cuts at: 6527 1700 Size: 2869 Fragments arranged by size: 2869 1744 1400 641 302 259 187 184 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7696 BssHII G'CGCG_C Cuts at: 1925 2921 1925 Size: 996 6700 BssSI C'ACGA_G Cuts at: 4601 5756 6768 4601 Size: 1155 1012 5529 Fragments arranged by size: 5529 1155 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2953 3565 3758 4054 794 Size: 2159 612 193 296 4436 Fragments arranged by size: 4436 2159 612 296 193 BstBI TT'CG_AA Cuts at: 2007 6500 2007 Size: 4493 3203 BstEII G'GTnAC_C Cuts at: 1184 2279 1184 Size: 1095 6601 BstXI CCAn_nnnn'nTGG Cuts at: 3784 4021 4735 3784 Size: 237 714 6745 Fragments arranged by size: 6745 714 237 BstYI r'GATC_y Cuts at: 1972 2697 2802 5026 5393 5778 5795 7247 7333 Size: 725 105 2224 367 385 17 1452 86 Cuts at: 7333 7345 7691 1972 Size: 12 346 1977 Fragments arranged by size: 2224 1977 1452 725 385 367 346 105 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4708 4708 Size: 7696 BtgI C'CryG_G Cuts at: 513 1065 2022 2034 2061 2326 2355 3186 4465 Size: 552 957 12 27 265 29 831 1279 Cuts at: 4465 4829 513 Size: 364 3380 Fragments arranged by size: 3380 1279 957 831 552 364 265 29 27 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2094 4012 4242 4325 4507 487 Size: 683 56 868 1918 230 83 182 3676 Fragments arranged by size: 3676 1918 868 683 230 182 83 56 BtsI GCAGTG_nn' Cuts at: 2251 2673 3067 3434 3677 4215 4310 5212 6006 Size: 422 394 367 243 538 95 902 794 Cuts at: 6006 6026 2251 Size: 20 3921 Fragments arranged by size: 3921 902 794 538 422 394 367 243 95 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7696 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4111 4146 529 Size: 35 3547 35 4079 Fragments arranged by size: 4079 3547 35 35 DraI TTT'AAA Cuts at: 5000 5000 Size: 7696 DraIII CAC_nnn'GTG Cuts at: 2246 2527 2566 4739 2246 Size: 281 39 2173 5203 Fragments arranged by size: 5203 2173 281 39 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4778 6703 809 Size: 507 3462 1925 1802 Fragments arranged by size: 3462 1925 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2104 3829 Size: 54 1425 50 275 18 207 67 1725 Cuts at: 3829 3876 4410 5143 6032 7661 8 Size: 47 534 733 889 1629 43 Fragments arranged by size: 1725 1629 1425 889 733 534 275 207 67 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3876 4410 5143 1537 Size: 275 2064 534 733 4090 Fragments arranged by size: 4090 2064 733 534 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2513 3138 4442 5626 1353 Size: 1160 625 1304 1184 3423 Fragments arranged by size: 3423 1304 1184 1160 625 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4102 6283 6657 6803 387 Size: 1064 138 2513 2181 374 146 1280 Fragments arranged by size: 2513 2181 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4273 4606 4273 Size: 333 7363 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2439 3083 3194 3317 3332 3446 Size: 393 695 112 644 111 123 15 114 Cuts at: 3446 3548 3589 3764 3783 3857 4055 4889 5759 Size: 102 41 175 19 74 198 834 870 Cuts at: 5759 6356 7143 7411 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 870 834 787 695 644 597 393 268 198 175 123 114 112 111 102 74 41 19 15 EcoNI CCTnn'n_nnAGG Cuts at: 2792 3467 3527 4862 2792 Size: 675 60 1335 5626 Fragments arranged by size: 5626 1335 675 60 EcoO109I rG'GnC_Cy Cuts at: 2066 3036 3037 3347 3460 3599 4318 4703 2066 Size: 970 1 310 113 139 719 385 5059 Fragments arranged by size: 5059 970 719 385 310 139 113 1 EcoRI G'AATT_C Cuts at: 4853 5005 5383 4853 Size: 152 378 7166 Fragments arranged by size: 7166 378 152 FseI GG_CCGG'CC Cuts at: 2116 2116 Size: 7696 FspI TGC'GCA Cuts at: 3500 3569 6202 3500 Size: 69 2633 4994 Fragments arranged by size: 4994 2633 69 HaeII r_GCGC'y Cuts at: 1678 2126 2142 2434 2652 2686 3293 3425 4002 Size: 448 16 292 218 34 607 132 577 Cuts at: 4002 4694 4754 4892 6843 1678 Size: 692 60 138 1951 2531 Fragments arranged by size: 2531 1951 692 607 577 448 292 218 138 132 60 34 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 6337 6369 6411 6443 1373 Size: 32 135 32 4765 32 42 32 2626 Fragments arranged by size: 4765 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3873 4555 5040 5286 669 Size: 988 281 1935 682 485 246 3079 Fragments arranged by size: 3079 1935 988 682 485 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7696 HpaI GTT'AAC Cuts at: 5286 5286 Size: 7696 KasI G'GCGC_C Cuts at: 4690 4750 4888 4690 Size: 60 138 7498 Fragments arranged by size: 7498 138 60 KpnI G_GTAC'C Cuts at: 5021 5021 Size: 7696 MfeI C'AATT_G Cuts at: 5295 5295 Size: 7696 MluI A'CGCG_T Cuts at: 7572 7572 Size: 7696 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2937 3522 6810 6994 1305 Size: 1632 585 3288 184 2007 Fragments arranged by size: 3288 2007 1632 585 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3831 10 Size: 54 1425 343 1999 3875 Fragments arranged by size: 3875 1999 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2477 2663 3654 4184 5654 6013 6172 Size: 648 1311 186 991 530 1470 359 159 Cuts at: 6172 518 Size: 2042 Fragments arranged by size: 2042 1470 1311 991 648 530 359 186 159 MspA1I CmG'CkG Cuts at: 1606 2036 2063 2328 2620 2863 3457 3514 3784 Size: 430 27 265 292 243 594 57 270 Cuts at: 3784 3816 4116 4249 4467 4677 4831 5112 5789 Size: 32 300 133 218 210 154 281 677 Cuts at: 5789 6937 7182 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 594 430 300 292 281 270 265 245 243 218 210 154 133 57 32 27 NaeI GCC'GGC Cuts at: 2114 2755 3014 4414 4598 2114 Size: 641 259 1400 184 5212 Fragments arranged by size: 5212 1400 641 259 184 NarI GG'CG_CC Cuts at: 4691 4751 4889 4691 Size: 60 138 7498 Fragments arranged by size: 7498 138 60 NcoI C'CATG_G Cuts at: 513 1065 2022 3186 513 Size: 552 957 1164 5023 Fragments arranged by size: 5023 1164 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7696 NgoMIV G'CCGG_C Cuts at: 2112 2753 3012 4412 4596 2112 Size: 641 259 1400 184 5212 Fragments arranged by size: 5212 1400 641 259 184 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7696 NotI GC'GGCC_GC Cuts at: 5143 5143 Size: 7696 NspI r_CATG'y Cuts at: 1132 1229 2929 5054 6599 1132 Size: 97 1700 2125 1545 2229 Fragments arranged by size: 2229 2125 1700 1545 97 PasI CC'CwG_GG Cuts at: 1348 3398 4591 1348 Size: 2050 1193 4453 Fragments arranged by size: 4453 2050 1193 PciI A'CATG_T Cuts at: 6595 6595 Size: 7696 PflMI CCAn_nnn'nTGG Cuts at: 1108 2527 2566 2781 4591 4768 1108 Size: 1419 39 215 1810 177 4036 Fragments arranged by size: 4036 1810 1419 215 177 39 PfoI T'CCnGG_A Cuts at: 2416 2805 3294 3630 4767 2416 Size: 389 489 336 1137 5345 Fragments arranged by size: 5345 1137 489 389 336 PmeI GTTT'AAAC Cuts at: 5000 5000 Size: 7696 PmlI CAC'GTG Cuts at: 2524 3379 2524 Size: 855 6841 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2171 2203 3611 3643 3923 3955 5765 5797 7278 Size: 32 1408 32 280 32 1810 32 1481 Cuts at: 7278 7310 2171 Size: 32 2557 Fragments arranged by size: 2557 1810 1481 1408 280 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2066 3599 4318 2066 Size: 1533 719 5444 Fragments arranged by size: 5444 1533 719 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7696 PsiI TTA'TAA Cuts at: 5266 5266 Size: 7696 PspOMI G'GGCC_C Cuts at: 3036 3459 4702 3036 Size: 423 1243 6030 Fragments arranged by size: 6030 1243 423 PstI C_TGCA'G Cuts at: 830 2625 2958 3180 3287 4050 4248 5048 830 Size: 1795 333 222 107 763 198 800 3478 Fragments arranged by size: 3478 1795 800 763 333 222 198 107 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7696 PvuII CAG'CTG Cuts at: 2620 2863 3514 4116 4249 2620 Size: 243 651 602 133 6067 Fragments arranged by size: 6067 651 602 243 133 SacI G_AGCT'C Cuts at: 4275 4608 4275 Size: 333 7363 SacII CC_GC'GG Cuts at: 2037 2064 2329 4468 4832 2037 Size: 27 265 2139 364 4901 Fragments arranged by size: 4901 2139 364 265 27 SalI G'TCGA_C Cuts at: 1936 5038 1936 Size: 3102 4594 SanDI GG'GwC_CC Cuts at: 2066 3599 2066 Size: 1533 6163 SbfI CC_TGCA'GG Cuts at: 2958 5048 2958 Size: 2090 5606 ScaI AGT'ACT Cuts at: 5944 7656 5944 Size: 1712 5984 SexAI A'CCwGG_T Cuts at: 2562 2562 Size: 7696 SfcI C'TryA_G Cuts at: 826 1045 2621 2954 3176 3283 4046 4244 5044 Size: 219 1576 333 222 107 763 198 800 Cuts at: 5044 6179 6860 7051 826 Size: 1135 681 191 1471 Fragments arranged by size: 1576 1471 1135 800 763 681 333 222 219 198 191 107 SfoI GGC'GCC Cuts at: 4692 4752 4890 4692 Size: 60 138 7498 Fragments arranged by size: 7498 138 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7696 SgrAI Cr'CCGG_yG Cuts at: 1700 2753 1700 Size: 1053 6643 SmaI CCC'GGG Cuts at: 1872 2195 2312 3391 4871 5023 1872 Size: 323 117 1079 1480 152 4545 Fragments arranged by size: 4545 1480 1079 323 152 117 SmlI C'TyrA_G Cuts at: 820 1017 1943 5800 6701 6963 820 Size: 197 926 3857 901 262 1553 Fragments arranged by size: 3857 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4993 5487 493 Size: 4500 494 2702 Fragments arranged by size: 4500 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7696 SphI G_CATG'C Cuts at: 1132 2929 5054 1132 Size: 1797 2125 3774 Fragments arranged by size: 3774 2125 1797 SspI AAT'ATT Cuts at: 5 52 5620 5 Size: 47 5568 2081 Fragments arranged by size: 5568 2081 47 StuI AGG'CCT Cuts at: 1517 3302 1517 Size: 1785 5911 StyI C'CwwG_G Cuts at: 513 1065 2022 3186 3826 3882 5135 513 Size: 552 957 1164 640 56 1253 3074 Fragments arranged by size: 3074 1253 1164 957 640 552 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2957 3238 4727 5723 5740 5893 6078 Size: 1255 1377 281 1489 996 17 153 185 Cuts at: 6078 6523 325 Size: 445 1498 Fragments arranged by size: 1498 1489 1377 1255 996 445 281 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2265 5942 7654 Size: 274 80 33 51 1443 288 3677 1712 Cuts at: 7654 96 Size: 138 Fragments arranged by size: 3677 1712 1443 288 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7656 7688 7656 Size: 32 7664 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7696 XbaI T'CTAG_A Cuts at: 5032 5032 Size: 7696 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7696 XmaI C'CCGG_G Cuts at: 1870 2193 2310 3389 4869 5021 1870 Size: 323 117 1079 1480 152 4545 Fragments arranged by size: 4545 1480 1079 323 152 117 XmnI GAAnn'nnTTC Cuts at: 5825 5825 Size: 7696 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3130 5504 276 Size: 53 83 186 936 1596 2374 2468 Fragments arranged by size: 2468 2374 1596 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI RsrII SapI SfiI SrfI SwaI XcmI