Restriction map for
pFN21ASDF0041
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# NO CUT ENZYMES ( 128 enzymes) #
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AccII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI BalI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglII BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tru9I Tsp509I TspRI XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 877 GACGTC GACGT^C
Acc65I 215 GGTACC G^GTACC
AccI 643 GTCTAC GT^[AC][GT]AC
AlwNI 682 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 171 GGGCCC GGGCC^C
Asp718I 215 GGTACC G^GTACC
AspHI 469 GAGCAC G[AT]GC[AT]^C
AspI 898 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 398 TGCGCA TGC^GCA
AvrII 78 CCTAGG C^CTAGG
BamHI 43 GGATCC G^GATCC
BbsI 880 GTCTTC GAAGAC(2/6)
BciVI 758 GGATAC GTATCC(6/5)
BfaI 79 CTAG C^TAG
BglI 16 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 78 CCTAGG C^CTAGG
BlpI 340 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 340 GCTCAGC GC^T[ACGT]AGC
BpuAI 880 GTCTTC GAAGAC(2/6)
BsaWI 84 TCCGGT [AT]^CCGG[AT]
BsiHKAI 469 GAGCAC G[AT]GC[AT]^C
BsmBI 875 GAGACG CGTCTC(1/5)
BstYI 43 GGATCC [GA]^GATC[CT]
CelII 340 GCTCAGC GC^T[ACGT]AGC
DraIII 190 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 398 TGCGCA TGC^GCA
KpnI 215 GGTACC GGTAC^C
MaeI 79 CTAG C^TAG
MflI 43 GGATCC [GA]^GATC[CT]
MslI 390 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 225 CTCGAG C^TCGAG
PflFI 898 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 284 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 30 CAGCTG CAG^CTG
SapI 51 GCTCTTC GCTCTTC(1/4)
SbfI 158 CCTGCAGG CCTGCA^GG
SfiI 15 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 225 CTCGAG C^T[CT][GA]AG
Sse8387I 158 CCTGCAGG CCTGCA^GG
TaqI 226 TCGA T^CGA
TfiI 611 GAATC G^A[AT]TC
Tth111I 898 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 226 TCGA T^CGA
XcmI 326 CCAGCCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 225 CTCGAG C^TCGAG
XhoII 43 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 4 GGCGTC G[GA]^CG[CT]C
877 GACGTC
AfaI 216 GTAC GT^AC
540 GTAC
AlwI 44 GATCC GGATC(4/5)
43 GGATC
BanII 171 GGGCCC G[GA]GC[CT]^C
841 GAGCCC
BcnI 727 CCCGG CC^[GC]GG
871 CCCGG
BsaHI 4 GGCGTC G[GA]^CG[CT]C
877 GACGTC
BseRI 549 CTCCTC GAGGAG(10/8)
860 CTCCTC
BsmAI 875 GAGAC GTCTC(1/5)
858 GTCTC
BsrFI 25 GCCGGC [GA]^CCGG[CT]
257 GCCGGC
BstXI 11 CCACGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
527 CCAACTGTGTGG
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
257 GCCGGC
DpnII 44 GATC ^GATC
450 GATC
EarI 52 CTCTTC CTCTTC(1/4)
61 CTCTTC
HgaI 5 GCGTC GACGC(5/10)
73 GCGTC
Hin1I 4 GGCGTC G[GA]^CG[CT]C
877 GACGTC
Ksp632I 52 CTCTTC CTCTTC(1/4)
61 CTCTTC
MaeIII 148 GTCAC ^GT[ACGT]AC
196 GTGAC
MboI 44 GATC ^GATC
450 GATC
MspA1I 30 CAGCTG C[AC]G^C[GT]G
802 CAGCGG
NaeI 25 GCCGGC GCC^GGC
257 GCCGGC
NciI 727 CCCGG CC^[GC]GG
871 CCCGG
NdeII 44 GATC ^GATC
450 GATC
NgoMIV 25 GCCGGC G^CCGGC
257 GCCGGC
RsaI 216 GTAC GT^AC
540 GTAC
Sau3AI 44 GATC ^GATC
450 GATC
Tsp45I 148 GTCAC ^GT[GC]AC
196 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AvaI AvaII BanI
BbvI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MboII MnlI MspI MvaI MwoI
NcoI NlaIII NlaIV PflMI PleI
PstI Sau96I ScrFI SfaNI SfcI
StyI TseI Van91I