Restriction map for
pFN21ASDF0041
(Linear) (Six-base) MAPSORT of: FLJ00041.seq Check: 9364 from: 1 to: 933
FLJ00041.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 166 302 489 756 933
Size: 166 136 187 267 177
Fragments arranged by size:
267 187 177 166 136
AatII G_ACGT'C
Cuts at: 0 881 933
Size: 881 52
AccI GT'mk_AC
Cuts at: 0 644 933
Size: 644 289
Acc65I G'GTAC_C
Cuts at: 0 215 933
Size: 215 718
AlwNI CAG_nnn'CTG
Cuts at: 0 687 933
Size: 687 246
ApaI G_GGCC'C
Cuts at: 0 175 933
Size: 175 758
AvaI C'yCGr_G
Cuts at: 0 133 225 763 933
Size: 133 92 538 170
Fragments arranged by size:
538 170 133 92
AvrII C'CTAG_G
Cuts at: 0 78 933
Size: 78 855
BamHI G'GATC_C
Cuts at: 0 43 933
Size: 43 890
BanI G'GyrC_C
Cuts at: 0 215 455 484 595 779 933
Size: 215 240 29 111 184 154
Fragments arranged by size:
240 215 184 154 111 29
BanII G_rGCy'C
Cuts at: 0 175 845 933
Size: 175 670 88
Fragments arranged by size:
670 175 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 873 933
Size: 873 60
BciVI GTATCCnnnnn_n'
Cuts at: 0 752 933
Size: 752 181
BglI GCCn_nnn'nGGC
Cuts at: 0 22 933
Size: 22 911
BlpI GC'TnA_GC
Cuts at: 0 341 933
Size: 341 592
Bme1580I G_kGCm'C
Cuts at: 0 175 191 681 933
Size: 175 16 490 252
Fragments arranged by size:
490 252 175 16
BmgBI CAC'GTC
Cuts at: 0 212 933
Size: 212 721
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 381 933
Size: 381 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 376 848 933
Size: 40 336 472 85
Fragments arranged by size:
472 336 85 40
BsaHI Gr'CG_yC
Cuts at: 0 5 878 933
Size: 5 873 55
Fragments arranged by size:
873 55 5
BsaWI w'CCGG_w
Cuts at: 0 84 933
Size: 84 849
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 933
Size: 532 30 371
Fragments arranged by size:
532 371 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 540 851 933
Size: 540 311 82
Fragments arranged by size:
540 311 82
BseYI C'CCAG_C
Cuts at: 0 38 800 844 933
Size: 38 762 44 89
Fragments arranged by size:
762 89 44 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 280 523 933
Size: 259 21 243 410
Fragments arranged by size:
410 259 243 21
BsiHKAI G_wGCw'C
Cuts at: 0 473 933
Size: 473 460
BsmBI CGTCTCn'nnnn_
Cuts at: 0 869 933
Size: 869 64
Bsp1286I G_dGCh'C
Cuts at: 0 175 191 473 681 845 933
Size: 175 16 282 208 164 88
Fragments arranged by size:
282 208 175 164 88 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 166 253 302 489 756 933
Size: 166 87 49 187 267 177
Fragments arranged by size:
267 187 177 166 87 49
BsrFI r'CCGG_y
Cuts at: 0 25 257 933
Size: 25 232 676
Fragments arranged by size:
676 232 25
BstXI CCAn_nnnn'nTGG
Cuts at: 0 18 534 933
Size: 18 516 399
Fragments arranged by size:
516 399 18
BstYI r'GATC_y
Cuts at: 0 43 933
Size: 43 890
BtgI C'CryG_G
Cuts at: 0 11 300 348 375 480 488 933
Size: 11 289 48 27 105 8 445
Fragments arranged by size:
445 289 105 48 27 11 8
DraIII CAC_nnn'GTG
Cuts at: 0 195 933
Size: 195 738
EarI CTCTTCn'nnn_
Cuts at: 0 58 67 933
Size: 58 9 866
Fragments arranged by size:
866 58 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 376 848 933
Size: 40 336 472 85
Fragments arranged by size:
472 336 85 40
EcoO109I rG'GnC_Cy
Cuts at: 0 172 285 868 933
Size: 172 113 583 65
Fragments arranged by size:
583 172 113 65
FspI TGC'GCA
Cuts at: 0 400 933
Size: 400 533
HaeII r_GCGC'y
Cuts at: 0 39 258 348 933
Size: 39 219 90 585
Fragments arranged by size:
585 219 90 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 508 540 933
Size: 35 32 441 32 393
Fragments arranged by size:
441 393 35 32 32
KpnI G_GTAC'C
Cuts at: 0 219 933
Size: 219 714
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 551 692 933
Size: 392 159 141 241
Fragments arranged by size:
392 241 159 141
MslI CAynn'nnrTG
Cuts at: 0 394 933
Size: 394 539
MspA1I CmG'CkG
Cuts at: 0 32 804 933
Size: 32 772 129
Fragments arranged by size:
772 129 32
NaeI GCC'GGC
Cuts at: 0 27 259 933
Size: 27 232 674
Fragments arranged by size:
674 232 27
NcoI C'CATG_G
Cuts at: 0 300 375 480 488 933
Size: 300 75 105 8 445
Fragments arranged by size:
445 300 105 75 8
NgoMIV G'CCGG_C
Cuts at: 0 25 257 933
Size: 25 232 676
Fragments arranged by size:
676 232 25
PasI CC'CwG_GG
Cuts at: 0 753 933
Size: 753 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 336 354 687 933
Size: 336 18 333 246
Fragments arranged by size:
336 333 246 18
PfoI T'CCnGG_A
Cuts at: 0 281 933
Size: 281 652
PpuMI rG'GwC_Cy
Cuts at: 0 285 933
Size: 285 648
PspOMI G'GGCC_C
Cuts at: 0 171 933
Size: 171 762
PspXI vC'TCGA_Gb
Cuts at: 0 225 933
Size: 225 708
PstI C_TGCA'G
Cuts at: 0 163 500 582 933
Size: 163 337 82 351
Fragments arranged by size:
351 337 163 82
PvuII CAG'CTG
Cuts at: 0 32 933
Size: 32 901
SapI GCTCTTCn'nnn_
Cuts at: 0 58 933
Size: 58 875
SbfI CC_TGCA'GG
Cuts at: 0 163 933
Size: 163 770
SfcI C'TryA_G
Cuts at: 0 159 496 578 933
Size: 159 337 82 355
Fragments arranged by size:
355 337 159 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 22 933
Size: 22 911
SmlI C'TyrA_G
Cuts at: 0 225 933
Size: 225 708
StyI C'CwwG_G
Cuts at: 0 78 300 375 480 488 933
Size: 78 222 75 105 8 445
Fragments arranged by size:
445 222 105 78 75 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 772 804 933
Size: 772 32 129
Fragments arranged by size:
772 129 32
Tth111I GACn'n_nGTC
Cuts at: 0 901 933
Size: 901 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 933
Size: 333 600
XhoI C'TCGA_G
Cuts at: 0 225 933
Size: 225 708
ZraI GAC'GTC
Cuts at: 0 879 933
Size: 879 54
Enzymes that do cut:
AarI AatII AccI Acc65I AlwNI ApaI AvaI AvrII
BamHI BanI BanII BbsI BciVI BglI BlpI Bme1580I
BmgBI BplI BpmI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstXI BstYI
BtgI DraIII EarI Eco57MI EcoO109I FspI HaeII Hin4I
KpnI MmeI MslI MspA1I NaeI NcoI NgoMIV PasI
PflMI PfoI PpuMI PspOMI PspXI PstI PvuII SapI
SbfI SfcI SfiI SmlI StyI TstI Tth111I XcmI
XhoI ZraI
Enzymes that do not cut:
AclI AcuI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI BaeI
BbeI BbvCI BcgI BclI BfrBI BglII BmrI BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
MfeI MluI MscI NarI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PsrI PvuI RsrII SacI SacII SalI SanDI
ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI XbaI
XmaI XmnI