(Circular) (Six-base) MAPSORT of: pFN21ASDF0018.seq Check: 5592 from: 1 to: 8887 pFN21ASDF0018 8887 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2443 3198 2443 Size: 755 8132 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6697 278 Size: 53 83 186 936 5161 2468 Fragments arranged by size: 5161 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4771 6230 1937 Size: 2834 1459 4594 Fragments arranged by size: 4594 2834 1459 Acc65I G'GTAC_C Cuts at: 5345 6208 5345 Size: 863 8024 AclI AA'CG_TT Cuts at: 1563 7014 7387 1563 Size: 5451 373 3063 Fragments arranged by size: 5451 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3672 3780 4182 5318 5766 6950 8334 8602 Size: 2040 108 402 1136 448 1184 1384 268 Cuts at: 8602 1632 Size: 1917 Fragments arranged by size: 2040 1917 1384 1184 1136 448 402 268 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8690 AflIII A'CryG_T Cuts at: 1203 4642 4676 4774 6177 7786 8763 1203 Size: 3439 34 98 1403 1609 977 1327 Fragments arranged by size: 3439 1609 1403 1327 977 98 34 AgeI A'CCGG_T Cuts at: 7718 7718 Size: 8887 AhdI GACnn_n'nnGTC Cuts at: 7616 7616 Size: 8887 AleI CACnn'nnGTG Cuts at: 1166 5132 1166 Size: 3966 4921 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2904 1184 Size: 1720 7167 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2293 2325 2963 2995 2293 Size: 32 638 32 8185 Fragments arranged by size: 8185 638 32 32 AlwNI CAG_nnn'CTG Cuts at: 2135 2219 2801 3584 3963 5228 5552 5830 6630 Size: 84 582 783 379 1265 324 278 800 Cuts at: 6630 8202 2135 Size: 1572 2820 Fragments arranged by size: 2820 1572 1265 800 783 582 379 324 278 84 ApaI G_GGCC'C Cuts at: 2226 2414 2795 3438 5222 5291 5837 5923 6151 Size: 188 381 643 1784 69 546 86 228 Cuts at: 6151 2226 Size: 4962 Fragments arranged by size: 4962 1784 643 546 381 228 188 86 69 ApaLI G'TGCA_C Cuts at: 6944 8100 6944 Size: 1156 7731 ApoI r'AATT_y Cuts at: 1493 3924 3951 3969 6196 6422 6574 8683 1493 Size: 2431 27 18 2227 226 152 2109 1697 Fragments arranged by size: 2431 2227 2109 1697 226 152 27 18 AseI AT'TA_AT Cuts at: 160 1032 7441 160 Size: 872 6409 1606 Fragments arranged by size: 6409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8887 AvaI C'yCGr_G Cuts at: 1870 1943 2072 2160 2343 2611 3135 3381 4444 Size: 73 129 88 183 268 524 246 1063 Cuts at: 4444 4467 4530 4563 5934 6212 1870 Size: 23 63 33 1371 278 4545 Fragments arranged by size: 4545 1371 1063 524 278 268 246 183 129 88 73 63 33 23 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5577 5610 5577 Size: 33 8854 BamHI G'GATC_C Cuts at: 2209 2301 4503 5278 5388 6217 6584 2209 Size: 92 2202 775 110 829 367 4512 Fragments arranged by size: 4512 2202 829 775 367 110 92 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2108 3742 4918 5345 6208 Size: 325 217 627 321 1634 1176 427 863 Cuts at: 6208 7663 618 Size: 1455 1842 Fragments arranged by size: 1842 1634 1455 1176 863 627 427 325 321 217 BanII G_rGCy'C Cuts at: 1391 2038 2177 2201 2226 2259 2414 2508 2618 Size: 647 139 24 25 33 155 94 110 Cuts at: 2618 2795 2946 3351 3438 3876 4444 4626 5171 Size: 177 151 405 87 438 568 182 545 Cuts at: 5171 5222 5291 5427 5837 5923 5942 6151 6160 Size: 51 69 136 410 86 19 209 9 Cuts at: 6160 6206 1391 Size: 46 4072 Fragments arranged by size: 4072 647 568 545 438 410 405 209 182 177 155 151 139 136 110 94 87 86 69 51 46 33 25 24 19 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5772 928 Size: 971 3873 4043 Fragments arranged by size: 4043 3873 971 BbvCI CC'TCA_GC Cuts at: 4131 4131 Size: 8887 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7078 7112 1903 Size: 34 5141 34 3678 Fragments arranged by size: 5141 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6779 7989 6779 Size: 1210 7677 BclI T'GATC_A Cuts at: 1548 4942 1548 Size: 3394 5493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4337 4761 6155 Size: 107 122 71 631 605 2665 424 1394 Cuts at: 6155 7498 136 Size: 1343 1525 Fragments arranged by size: 2665 1525 1394 1343 631 605 424 122 107 71 BglII A'GATC_T Cuts at: 8882 8882 Size: 8887 BlpI GC'TnA_GC Cuts at: 2239 2441 3510 6020 6304 2239 Size: 202 1069 2510 284 4822 Fragments arranged by size: 4822 2510 1069 284 202 Bme1580I G_kGCm'C Cuts at: 1790 2226 2414 2462 2795 3325 3438 3939 4684 Size: 436 188 48 333 530 113 501 745 Cuts at: 4684 5139 5222 5291 5837 5923 6151 6948 8104 Size: 455 83 69 546 86 228 797 1156 Cuts at: 8104 1790 Size: 2573 Fragments arranged by size: 2573 1156 797 745 546 530 501 455 436 333 228 188 113 86 83 69 48 BmgBI CAC'GTC Cuts at: 1318 3862 3934 5961 1318 Size: 2544 72 2027 4244 Fragments arranged by size: 4244 2544 2027 72 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2791 2811 4059 Size: 416 53 83 399 24 1374 20 1248 Cuts at: 4059 4193 5705 6618 7576 442 Size: 134 1512 913 958 1753 Fragments arranged by size: 1753 1512 1374 1248 958 913 416 399 134 83 53 24 20 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2214 2230 2351 2531 2885 3098 3179 3224 Size: 975 16 121 180 354 213 81 45 Cuts at: 3224 3540 3623 3848 4122 4415 4854 4898 5064 Size: 316 83 225 274 293 439 44 166 Cuts at: 5064 5839 7547 1239 Size: 775 1708 2579 Fragments arranged by size: 2579 1708 975 775 439 354 316 293 274 225 213 180 166 121 83 81 45 44 16 Bpu10I CC'TnA_GC Cuts at: 2186 3116 4131 4791 4839 4989 5040 5752 2186 Size: 930 1015 660 48 150 51 712 5321 Fragments arranged by size: 5321 1015 930 712 660 150 51 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2308 2729 7012 7877 8175 2308 Size: 421 4283 865 298 3020 Fragments arranged by size: 4283 3020 865 421 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3132 3375 7550 882 Size: 617 1633 243 4175 2219 Fragments arranged by size: 4175 2219 1633 617 243 BsaAI yAC'GTr Cuts at: 493 1204 4645 6049 6184 6678 493 Size: 711 3441 1404 135 494 2702 Fragments arranged by size: 3441 2702 1404 711 494 135 BsaBI GATnn'nnATC Cuts at: 4404 8881 4404 Size: 4477 4410 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4463 5792 6694 7076 Size: 53 83 186 936 2930 1329 902 382 Cuts at: 7076 7711 275 Size: 635 1451 Fragments arranged by size: 2930 1451 1329 936 902 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2293 2933 4323 5566 6090 7320 7718 7992 8139 Size: 640 1390 1243 524 1230 398 274 147 Cuts at: 8139 8550 2293 Size: 411 2630 Fragments arranged by size: 2630 1390 1243 1230 640 524 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2600 2627 2630 2657 2963 Size: 30 165 30 1197 27 3 27 306 Cuts at: 2963 2993 3177 3207 3254 3284 1178 Size: 30 184 30 47 30 6781 Fragments arranged by size: 6781 1197 306 184 165 47 30 30 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2581 3000 3363 3818 3821 3824 4235 5688 Size: 1395 419 363 455 3 3 411 1453 Cuts at: 5688 1186 Size: 4385 Fragments arranged by size: 4385 1453 1395 455 419 411 363 3 3 BseYI C'CCAG_C Cuts at: 2685 2920 3782 3902 3956 4581 5392 5985 5991 Size: 235 862 120 54 625 811 593 6 Cuts at: 5991 8090 8586 2685 Size: 2099 496 2986 Fragments arranged by size: 2986 2099 862 811 625 593 496 235 120 54 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2582 2930 3570 4892 5346 5538 1163 Size: 573 846 348 640 1322 454 192 4512 Fragments arranged by size: 4512 1322 846 640 573 454 348 192 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6337 7098 8126 1540 Size: 275 186 4336 761 1028 2301 Fragments arranged by size: 4336 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2177 2192 2508 2618 2946 5427 6948 7033 8104 Size: 15 316 110 328 2481 1521 85 1071 Cuts at: 8104 2177 Size: 2960 Fragments arranged by size: 2960 2481 1521 1071 328 316 110 85 15 BsmI GAATG_Cn' Cuts at: 2739 3906 4301 6398 6491 2739 Size: 1167 395 2097 93 5135 Fragments arranged by size: 5135 2097 1167 395 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2177 2192 2201 2226 2259 2414 Size: 399 248 139 15 9 25 33 155 Cuts at: 2414 2462 2508 2618 2795 2946 3325 3351 3438 Size: 48 46 110 177 151 379 26 87 Cuts at: 3438 3876 3939 4444 4626 4684 5139 5171 5222 Size: 438 63 505 182 58 455 32 51 Cuts at: 5222 5291 5427 5837 5923 5942 6151 6160 6206 Size: 69 136 410 86 19 209 9 46 Cuts at: 6206 6948 7033 8104 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 505 455 438 410 399 379 248 209 182 177 155 151 139 136 110 87 86 85 69 63 58 51 48 46 46 33 32 26 25 19 15 9 9 BspEI T'CCGG_A Cuts at: 4323 5566 8550 4323 Size: 1243 2984 4660 Fragments arranged by size: 4660 2984 1243 BspHI T'CATG_A Cuts at: 6776 8506 6776 Size: 1730 7157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2443 3198 6242 844 Size: 1599 755 3044 3489 Fragments arranged by size: 3489 3044 1599 755 BsrBI CCG'CTC Cuts at: 1394 1823 3025 4308 4428 4501 6774 1394 Size: 429 1202 1283 120 73 2273 3507 Fragments arranged by size: 3507 2273 1283 1202 429 120 73 BsrDI GCAATG_nn' Cuts at: 65 1248 3885 7382 7556 65 Size: 1183 2637 3497 174 1396 Fragments arranged by size: 3497 2637 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3472 4557 4921 7531 7718 1700 Size: 110 1662 1085 364 2610 187 2869 Fragments arranged by size: 2869 2610 1662 1085 364 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8887 BssHII G'CGCG_C Cuts at: 1925 2894 1925 Size: 969 7918 BssSI C'ACGA_G Cuts at: 6947 7959 6947 Size: 1012 7875 BstAPI GCAn_nnn'nTGC Cuts at: 794 3912 794 Size: 3118 5769 BstBI TT'CG_AA Cuts at: 2007 7691 2007 Size: 5684 3203 BstEII G'GTnAC_C Cuts at: 1184 5150 1184 Size: 3966 4921 BstXI CCAn_nnnn'nTGG Cuts at: 3746 5863 3746 Size: 2117 6770 BstYI r'GATC_y Cuts at: 1972 2209 2301 3691 4405 4503 5278 5388 6217 Size: 237 92 1390 714 98 775 110 829 Cuts at: 6217 6584 6969 6986 8438 8524 8536 8882 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1390 829 775 714 385 367 346 237 110 98 92 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2028 3594 2028 Size: 1566 7321 BtgI C'CryG_G Cuts at: 513 1065 2148 5724 513 Size: 552 1083 3576 3676 Fragments arranged by size: 3676 3576 1083 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8148 Fragments arranged by size: 8148 683 56 BtsI GCAGTG_nn' Cuts at: 4382 6403 7197 7217 4382 Size: 2021 794 20 6052 Fragments arranged by size: 6052 2021 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5394 5429 529 Size: 35 4830 35 3987 Fragments arranged by size: 4830 3987 35 35 DraI TTT'AAA Cuts at: 6191 6191 Size: 8887 DraIII CAC_nnn'GTG Cuts at: 2115 2115 Size: 8887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2429 3488 5489 7894 809 Size: 507 1113 1059 2001 2405 1802 Fragments arranged by size: 2405 2001 1802 1113 1059 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 3060 4364 Size: 54 1425 50 275 18 250 980 1304 Cuts at: 4364 4762 6071 6334 7223 8852 8 Size: 398 1309 263 889 1629 43 Fragments arranged by size: 1629 1425 1309 1304 980 889 398 275 263 250 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6334 1537 Size: 275 4522 4090 Fragments arranged by size: 4522 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2080 3038 3207 3212 3254 3795 3799 3810 Size: 727 958 169 5 42 541 4 11 Cuts at: 3810 5618 6817 1353 Size: 1808 1199 3423 Fragments arranged by size: 3423 1808 1199 958 727 541 169 42 11 5 4 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2267 2640 2801 3006 4538 7474 Size: 1064 138 678 373 161 205 1532 2936 Cuts at: 7474 7848 7994 387 Size: 374 146 1280 Fragments arranged by size: 2936 1532 1280 1064 678 374 373 205 161 146 138 EcoICRI GAG'CTC Cuts at: 2175 2506 2616 2944 5425 2175 Size: 331 110 328 2481 5637 Fragments arranged by size: 5637 2481 331 328 110 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2214 2230 2351 2531 2885 3098 3179 Size: 393 582 16 121 180 354 213 81 Cuts at: 3179 3224 3540 3623 3672 3780 3848 4122 4182 Size: 45 316 83 49 108 68 274 60 Cuts at: 4182 4415 4854 4898 5064 5318 5766 5839 6950 Size: 233 439 44 166 254 448 73 1111 Cuts at: 6950 7547 8334 8602 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1111 787 597 582 448 439 393 354 316 274 268 254 233 213 180 166 121 108 83 81 73 68 60 49 45 44 16 EcoNI CCTnn'n_nnAGG Cuts at: 2374 3194 4200 4842 5670 5913 2374 Size: 820 1006 642 828 243 5348 Fragments arranged by size: 5348 1006 828 820 642 243 EcoO109I rG'GnC_Cy Cuts at: 2267 2380 2411 2431 2791 3435 3481 3598 3639 Size: 113 31 20 360 644 46 117 41 Cuts at: 3639 3845 4112 4703 4901 5218 5287 5695 5834 Size: 206 267 591 198 317 69 408 139 Cuts at: 5834 5906 5919 5920 6147 2267 Size: 72 13 1 227 5007 Fragments arranged by size: 5007 644 591 408 360 317 267 227 206 198 139 117 113 72 69 46 41 31 20 13 1 EcoRI G'AATT_C Cuts at: 6196 6574 6196 Size: 378 8509 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3317 3317 Size: 8887 FspI TGC'GCA Cuts at: 7393 7393 Size: 8887 HaeII r_GCGC'y Cuts at: 1678 2538 2680 3247 4332 8034 1678 Size: 860 142 567 1085 3702 2531 Fragments arranged by size: 3702 2531 1085 860 567 142 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2034 2066 2928 2960 3115 Size: 32 135 32 462 32 862 32 155 Cuts at: 3115 3147 3177 3209 3220 3252 5782 5814 7528 Size: 32 30 32 11 32 2530 32 1714 Cuts at: 7528 7560 7602 7634 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2530 1714 862 462 155 135 42 32 32 32 32 32 32 32 32 32 32 30 11 HincII GTy'rAC Cuts at: 669 1657 1938 5240 6040 6231 6477 669 Size: 988 281 3302 800 191 246 3079 Fragments arranged by size: 3302 3079 988 800 281 246 191 HindIII A'AGCT_T Cuts at: 748 748 Size: 8887 HpaI GTT'AAC Cuts at: 6040 6477 6040 Size: 437 8450 KpnI G_GTAC'C Cuts at: 5349 6212 5349 Size: 863 8024 MfeI C'AATT_G Cuts at: 6486 6486 Size: 8887 MluI A'CGCG_T Cuts at: 8763 8763 Size: 8887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2949 5595 5754 8001 8185 1305 Size: 1644 2646 159 2247 184 2007 Fragments arranged by size: 2646 2247 2007 1644 184 159 MscI TGG'CCA Cuts at: 10 64 1489 1832 3062 4764 10 Size: 54 1425 343 1230 1702 4133 Fragments arranged by size: 4133 1702 1425 1230 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4675 5132 6047 6845 7204 7363 518 Size: 648 3509 457 915 798 359 159 2042 Fragments arranged by size: 3509 2042 915 798 648 457 359 159 MspA1I CmG'CkG Cuts at: 1606 2846 2914 3893 3963 4130 4357 4422 4519 Size: 1240 68 979 70 167 227 65 97 Cuts at: 4519 4823 5549 5651 5846 6303 6980 8128 8373 Size: 304 726 102 195 457 677 1148 245 Cuts at: 8373 1606 Size: 2120 Fragments arranged by size: 2120 1240 1148 979 726 677 457 304 245 227 195 167 102 97 70 68 65 NaeI GCC'GGC Cuts at: 4559 4923 4559 Size: 364 8523 NcoI C'CATG_G Cuts at: 513 1065 2148 5724 513 Size: 552 1083 3576 3676 Fragments arranged by size: 3676 3576 1083 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8887 NgoMIV G'CCGG_C Cuts at: 4557 4921 4557 Size: 364 8523 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8887 NotI GC'GGCC_GC Cuts at: 6334 6334 Size: 8887 NruI TCG'CGA Cuts at: 2811 2811 Size: 8887 NspI r_CATG'y Cuts at: 1132 1229 2891 4680 4778 4862 6181 6245 7790 Size: 97 1662 1789 98 84 1319 64 1545 Cuts at: 7790 1132 Size: 2229 Fragments arranged by size: 2229 1789 1662 1545 1319 98 97 84 64 PasI CC'CwG_GG Cuts at: 1348 2219 3404 4699 5284 6151 1348 Size: 871 1185 1295 585 867 4084 Fragments arranged by size: 4084 1295 1185 871 867 585 PciI A'CATG_T Cuts at: 4676 4774 6177 7786 4676 Size: 98 1403 1609 5777 Fragments arranged by size: 5777 1609 1403 98 PflMI CCAn_nnn'nTGG Cuts at: 1108 2219 2801 3431 3865 5890 1108 Size: 1111 582 630 434 2025 4105 Fragments arranged by size: 4105 2025 1111 630 582 434 PfoI T'CCnGG_A Cuts at: 2508 2631 2901 3660 3864 4392 2508 Size: 123 270 759 204 528 7003 Fragments arranged by size: 7003 759 528 270 204 123 PmeI GTTT'AAAC Cuts at: 6191 6191 Size: 8887 PmlI CAC'GTG Cuts at: 4645 6049 4645 Size: 1404 7483 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2963 2995 6956 6988 8469 8501 2963 Size: 32 3961 32 1481 32 3349 Fragments arranged by size: 3961 3349 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2380 2431 3481 3598 3639 4703 2380 Size: 51 1050 117 41 1064 6564 Fragments arranged by size: 6564 1064 1050 117 51 41 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8887 PsiI TTA'TAA Cuts at: 6457 6457 Size: 8887 PspOMI G'GGCC_C Cuts at: 2222 2410 2791 3434 5218 5287 5833 5919 6147 Size: 188 381 643 1784 69 546 86 228 Cuts at: 6147 2222 Size: 4962 Fragments arranged by size: 4962 1784 643 546 381 228 188 86 69 PstI C_TGCA'G Cuts at: 830 2140 2673 2712 2766 4122 5237 6239 830 Size: 1310 533 39 54 1356 1115 1002 3478 Fragments arranged by size: 3478 1356 1310 1115 1002 533 54 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8887 PvuII CAG'CTG Cuts at: 3893 4130 4357 4823 5651 5846 3893 Size: 237 227 466 828 195 6934 Fragments arranged by size: 6934 828 466 237 227 195 SacI G_AGCT'C Cuts at: 2177 2508 2618 2946 5427 2177 Size: 331 110 328 2481 5637 Fragments arranged by size: 5637 2481 331 328 110 SalI G'TCGA_C Cuts at: 1936 6229 1936 Size: 4293 4594 SapI GCTCTTCn'nnn_ Cuts at: 3038 5618 3038 Size: 2580 6307 SbfI CC_TGCA'GG Cuts at: 6239 6239 Size: 8887 ScaI AGT'ACT Cuts at: 7135 8847 7135 Size: 1712 7175 SfcI C'TryA_G Cuts at: 826 1045 2136 2669 2708 2762 4118 5233 6235 Size: 219 1091 533 39 54 1356 1115 1002 Cuts at: 6235 7370 8051 8242 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1356 1135 1115 1091 1002 681 533 219 191 54 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8887 SmaI CCC'GGG Cuts at: 1872 2345 5936 6214 1872 Size: 473 3591 278 4545 Fragments arranged by size: 4545 3591 473 278 SmlI C'TyrA_G Cuts at: 820 1017 1943 2323 2744 3381 6991 7892 8154 Size: 197 926 380 421 637 3610 901 262 Cuts at: 8154 820 Size: 1553 Fragments arranged by size: 3610 1553 926 901 637 421 380 262 197 SnaBI TAC'GTA Cuts at: 493 6184 6678 493 Size: 5691 494 2702 Fragments arranged by size: 5691 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8887 SphI G_CATG'C Cuts at: 1132 2891 6245 1132 Size: 1759 3354 3774 Fragments arranged by size: 3774 3354 1759 SspI AAT'ATT Cuts at: 5 52 6811 5 Size: 47 6759 2081 Fragments arranged by size: 6759 2081 47 StuI AGG'CCT Cuts at: 1517 3684 1517 Size: 2167 6720 StyI C'CwwG_G Cuts at: 513 1065 2038 2148 3282 4108 5091 5724 6078 Size: 552 973 110 1134 826 983 633 354 Cuts at: 6078 6326 513 Size: 248 3074 Fragments arranged by size: 3074 1134 983 973 826 633 552 354 248 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2912 3722 4286 4400 4968 5527 5719 Size: 1255 1332 810 564 114 568 559 192 Cuts at: 5719 6914 6931 7084 7269 7714 325 Size: 1195 17 153 185 445 1498 Fragments arranged by size: 1498 1332 1255 1195 810 568 564 559 445 192 185 153 114 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 3081 7133 Size: 274 80 33 51 1443 618 486 4052 Cuts at: 7133 8845 96 Size: 1712 138 Fragments arranged by size: 4052 1712 1443 618 486 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3254 3286 3846 3878 4374 4406 5425 5457 8847 Size: 32 560 32 496 32 1019 32 3390 Cuts at: 8847 8879 3254 Size: 32 3262 Fragments arranged by size: 3390 3262 1019 560 496 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4462 5228 1316 Size: 3146 766 4975 Fragments arranged by size: 4975 3146 766 XbaI T'CTAG_A Cuts at: 5683 6223 5683 Size: 540 8347 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2661 2661 Size: 8887 XhoI C'TCGA_G Cuts at: 1943 3381 1943 Size: 1438 7449 XmaI C'CCGG_G Cuts at: 1870 2343 5934 6212 1870 Size: 473 3591 278 4545 Fragments arranged by size: 4545 3591 473 278 XmnI GAAnn'nnTTC Cuts at: 7016 7016 Size: 8887 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6695 276 Size: 53 83 186 936 5161 2468 Fragments arranged by size: 5161 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI