Restriction map for
pFN21AB9404
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspHI
AspI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HpaI KasI KpnI KspI MluI
MluNI MroI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 974 GTAGAC GT^[AC][GT]AC
AccII 218 CGCG CG^CG
AcsI 402 GAATTT [GA]^AATT[CT]
AcyI 1751 GACGCC G[GA]^CG[CT]C
AflIII 1878 ACATGT A^C[GA][CT]GT
AhdI 2120 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 402 GAATTT [GA]^AATT[CT]
AspEI 2120 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 896 CTCGGG C^[CT]CG[GA]G
BclI 966 TGATCA T^GATCA
BpmI 2024 CTGGAG CTGGAG(16/14)
BsaHI 1751 GACGCC G[GA]^CG[CT]C
BsaWI 1611 ACCGGA [AT]^CCGG[AT]
BsgI 992 GTGCAG GTGCAG(16/14)
BsoBI 896 CTCGGG C^[CT]CG[GA]G
BspHI 1968 TCATGA T^CATGA
BspLU11I 1878 ACATGT A^CATGT
BspMI 246 GCAGGT ACCTGC(4/8)
BstAPI 285 GCAGAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1289 TTCGAA TT^CGAA
BstUI 218 CGCG CG^CG
BstXI 1130 CCATTGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 219 GCGC GCG^C
DraII 2141 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 2120 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2141 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 966 TGATCA T^GATCA
HaeIII 710 GGCC GG^CC
HgaI 1751 GACGC GACGC(5/10)
HhaI 219 GCGC GCG^C
Hin1I 1751 GACGCC G[GA]^CG[CT]C
HinP1I 219 GCGC G^CGC
HincII 436 GTCAAC GT[CT]^[GA]AC
HindII 436 GTCAAC GT[CT]^[GA]AC
HindIII 1669 AAGCTT A^AGCTT
MaeII 2379 ACGT A^CGT
MvnI 218 CGCG CG^CG
NspV 1289 TTCGAA TT^CGAA
PflMI 772 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2141 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1968 TCATGA T^CATGA
SapI 763 GCTCTTC GCTCTTC(1/4)
SfcI 987 CTACAG C^T[GA][CT]AG
SfuI 1289 TTCGAA TT^CGAA
SspI 980 AATATT AAT^ATT
Tsp45I 214 GTCAC ^GT[GC]AC
Van91I 772 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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Asp700I 2005 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2344 GAATTAATTC
BamHI 1659 GGATCC G^GATCC
1807 GGATCC
BanII 783 GAGCCC G[GA]GC[CT]^C
2027 GAGCCC
BbsI 1197 GTCTTC GAAGAC(2/6)
1305 GTCTTC
BcgI 275 CGAAAATAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1764 GCAAGCTGTTCG
BcnI 541 CCCGG CC^[GC]GG
786 CCCGG
BfaI 572 CTAG C^TAG
1691 CTAG
BmyI 783 GAGCCC G[AGT]GC[ACT]^C
2027 GAGCCC
BpuAI 1197 GTCTTC GAAGAC(2/6)
1305 GTCTTC
BsaBI 391 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1225 GATACAGATC
BsmAI 1119 GAGAC GTCTC(1/5)
2296 GAGAC
BsmFI 859 GGGAC GGGAC(10/14)
2270 GTCCC
Bsp1286I 783 GAGCCC G[AGT]GC[ACT]^C
2027 GAGCCC
DraI 1543 TTTAAA TTT^AAA
1681 TTTAAA
MaeI 572 CTAG C^TAG
1691 CTAG
MaeIII 214 GTCAC ^GT[ACGT]AC
1258 GTAAC
MamI 391 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1225 GATACAGATC
MfeI 1427 CAATTG C^AATTG
2258 CAATTG
MunI 1427 CAATTG C^AATTG
2258 CAATTG
MwoI 153 GCAAGTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
285 GCAGAGTTTGC
NciI 541 CCCGG CC^[GC]GG
786 CCCGG
NspI 957 GCATGT [GA]CATG^[CT]
1878 ACATGT
XmnI 2005 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2344 GAATTAATTC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AseI
AsnI AvaII BbvI BsaJI BseRI
BsiYI BslI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HapII HinfI HpaII HphI ItaI
Ksp632I MboI MboII MflI MnlI
MseI MslI MspI MvaI NdeII
NlaIII NlaIV PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII