(Circular) (Six-base) MAPSORT of: pFN21AB9404.seq Check: 5304 from: 1 to: 7111 pFN21AB9404 7111 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4921 278 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2998 4454 1937 Size: 1061 1456 4594 Fragments arranged by size: 4594 1456 1061 Acc65I G'GTAC_C Cuts at: 4432 4432 Size: 7111 AclI AA'CG_TT Cuts at: 1563 5238 5611 1563 Size: 3675 373 3063 Fragments arranged by size: 3675 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2306 3711 5174 6558 6826 1632 Size: 674 1405 1463 1384 268 1917 Fragments arranged by size: 1917 1463 1405 1384 674 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6914 AflIII A'CryG_T Cuts at: 1203 3901 6010 6987 1203 Size: 2698 2109 977 1327 Fragments arranged by size: 2698 2109 1327 977 AgeI A'CCGG_T Cuts at: 5942 5942 Size: 7111 AhdI GACnn_n'nnGTC Cuts at: 4148 5840 4148 Size: 1692 5419 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7111 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7111 AlwNI CAG_nnn'CTG Cuts at: 4854 6426 4854 Size: 1572 5539 ApaLI G'TGCA_C Cuts at: 5168 6324 5168 Size: 1156 5955 ApoI r'AATT_y Cuts at: 1493 2425 4420 4646 4798 6907 1493 Size: 932 1995 226 152 2109 1697 Fragments arranged by size: 2109 1995 1697 932 226 152 AseI AT'TA_AT Cuts at: 160 1032 2823 3555 4370 5665 160 Size: 872 1791 732 815 1295 1606 Fragments arranged by size: 1791 1606 1295 872 815 732 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 AvaI C'yCGr_G Cuts at: 1870 1943 2919 4436 1870 Size: 73 976 1517 4545 Fragments arranged by size: 4545 1517 976 73 BamHI G'GATC_C Cuts at: 3682 3830 4441 4808 3682 Size: 148 611 367 5985 Fragments arranged by size: 5985 611 367 148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4432 5887 618 Size: 325 217 627 2645 1455 1842 Fragments arranged by size: 2645 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2810 4054 4430 1391 Size: 1419 1244 376 4072 Fragments arranged by size: 4072 1419 1244 376 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3213 3321 928 Size: 971 1314 108 4718 Fragments arranged by size: 4718 1314 971 108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2287 2321 3776 3810 5302 5336 1903 Size: 34 350 34 1455 34 1492 34 3678 Fragments arranged by size: 3678 1492 1455 350 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5003 6213 5003 Size: 1210 5901 BclI T'GATC_A Cuts at: 1548 2989 1548 Size: 1441 5670 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5722 136 Size: 107 122 71 631 605 4050 1525 Fragments arranged by size: 4050 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7106 7106 Size: 7111 BlpI GC'TnA_GC Cuts at: 4528 4528 Size: 7111 Bme1580I G_kGCm'C Cuts at: 1790 5172 6328 1790 Size: 3382 1156 2573 Fragments arranged by size: 3382 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7111 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2215 2430 2773 Size: 416 53 83 399 24 798 215 343 Cuts at: 2773 4842 5800 442 Size: 2069 958 1753 Fragments arranged by size: 2069 1753 958 798 416 399 343 215 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4068 5771 1239 Size: 2829 1703 2579 Fragments arranged by size: 2829 2579 1703 Bpu10I CC'TnA_GC Cuts at: 2355 2465 2854 2355 Size: 110 389 6612 Fragments arranged by size: 6612 389 110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2960 3360 3422 5236 6101 6399 2960 Size: 400 62 1814 865 298 3672 Fragments arranged by size: 3672 1814 865 400 298 62 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5774 882 Size: 617 4275 2219 Fragments arranged by size: 4275 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4408 4902 493 Size: 711 3204 494 2702 Fragments arranged by size: 3204 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2418 3252 7105 2418 Size: 834 3853 2424 Fragments arranged by size: 3853 2424 834 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3775 4918 5300 5935 Size: 53 83 186 936 2242 1143 382 635 Cuts at: 5935 275 Size: 1451 Fragments arranged by size: 2242 1451 1143 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3634 5544 5942 6216 6363 6774 3634 Size: 1910 398 274 147 411 3971 Fragments arranged by size: 3971 1910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2701 2731 1178 Size: 30 165 30 1298 30 5558 Fragments arranged by size: 5558 1298 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2898 3220 3641 1186 Size: 1712 322 421 4656 Fragments arranged by size: 4656 1712 421 322 BseYI C'CCAG_C Cuts at: 3066 6314 6810 3066 Size: 3248 496 3367 Fragments arranged by size: 3367 3248 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3036 1163 Size: 573 1300 5238 Fragments arranged by size: 5238 1300 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4561 5322 6350 1540 Size: 275 186 2560 761 1028 2301 Fragments arranged by size: 2560 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5172 5257 6328 5172 Size: 85 1071 5955 Fragments arranged by size: 5955 1071 85 BsmI GAATG_Cn' Cuts at: 4622 4715 4622 Size: 93 7018 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2810 4054 4430 5172 5257 6328 1391 Size: 399 1020 1244 376 742 85 1071 2174 Fragments arranged by size: 2174 1244 1071 1020 742 399 376 85 BspEI T'CCGG_A Cuts at: 6774 6774 Size: 7111 BspHI T'CATG_A Cuts at: 3991 5000 6730 3991 Size: 1009 1730 4372 Fragments arranged by size: 4372 1730 1009 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2260 4466 844 Size: 1416 2206 3489 Fragments arranged by size: 3489 2206 1416 BsrBI CCG'CTC Cuts at: 1394 1823 4998 1394 Size: 429 3175 3507 Fragments arranged by size: 3507 3175 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5606 5780 65 Size: 1183 4358 174 1396 Fragments arranged by size: 4358 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5755 5942 1700 Size: 110 3945 187 2869 Fragments arranged by size: 3945 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7111 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7111 BssSI C'ACGA_G Cuts at: 5171 6183 5171 Size: 1012 6099 BstAPI GCAn_nnn'nTGC Cuts at: 794 2314 794 Size: 1520 5591 BstBI TT'CG_AA Cuts at: 2007 3313 5915 2007 Size: 1306 2602 3203 Fragments arranged by size: 3203 2602 1306 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7111 BstXI CCAn_nnnn'nTGG Cuts at: 2029 3160 2029 Size: 1131 5980 BstYI r'GATC_y Cuts at: 1972 3023 3682 3830 4441 4808 5193 5210 6662 Size: 1051 659 148 611 367 385 17 1452 Cuts at: 6662 6748 6760 7106 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1051 659 611 385 367 346 148 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6559 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6372 Fragments arranged by size: 6372 683 56 BtsI GCAGTG_nn' Cuts at: 3966 4627 5421 5441 3966 Size: 661 794 20 5636 Fragments arranged by size: 5636 794 661 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7111 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7076 DraI TTT'AAA Cuts at: 3568 3706 4415 3568 Size: 138 709 6264 Fragments arranged by size: 6264 709 138 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6118 809 Size: 507 4802 1802 Fragments arranged by size: 4802 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4558 5447 7076 Size: 54 1425 50 275 18 2728 889 1629 Cuts at: 7076 8 Size: 43 Fragments arranged by size: 2728 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4558 1537 Size: 275 2746 4090 Fragments arranged by size: 4090 2746 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2793 3618 4225 5041 1353 Size: 1440 825 607 816 3423 Fragments arranged by size: 3423 1440 825 816 607 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5698 6072 6218 387 Size: 1064 138 4109 374 146 1280 Fragments arranged by size: 4109 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2306 3711 4068 5174 5771 6558 6826 Size: 393 674 1405 357 1106 597 787 268 Cuts at: 6826 1239 Size: 1524 Fragments arranged by size: 1524 1405 1106 787 674 597 393 357 268 EcoO109I rG'GnC_Cy Cuts at: 4165 4165 Size: 7111 EcoRI G'AATT_C Cuts at: 4420 4798 4420 Size: 378 6733 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3769 3769 Size: 7111 FspI TGC'GCA Cuts at: 5617 5617 Size: 7111 HaeII r_GCGC'y Cuts at: 1678 6258 1678 Size: 4580 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2180 2212 2302 2334 2648 Size: 32 135 32 608 32 90 32 314 Cuts at: 2648 2680 3164 3196 3822 3854 4134 4166 5752 Size: 32 484 32 626 32 280 32 1586 Cuts at: 5752 5784 5826 5858 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1586 626 608 484 314 280 135 90 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2461 4455 4701 669 Size: 988 281 523 1994 246 3079 Fragments arranged by size: 3079 1994 988 523 281 246 HindIII A'AGCT_T Cuts at: 748 3692 748 Size: 2944 4167 HpaI GTT'AAC Cuts at: 4701 4701 Size: 7111 KpnI G_GTAC'C Cuts at: 4436 4436 Size: 7111 MfeI C'AATT_G Cuts at: 3450 4281 4710 3450 Size: 831 429 5851 Fragments arranged by size: 5851 831 429 MluI A'CGCG_T Cuts at: 6987 6987 Size: 7111 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2894 3209 4243 6225 6409 1305 Size: 1589 315 1034 1982 184 2007 Fragments arranged by size: 2007 1982 1589 1034 315 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5289 Fragments arranged by size: 5289 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2147 2985 4107 5069 5428 5587 Size: 648 861 120 838 1122 962 359 159 Cuts at: 5587 518 Size: 2042 Fragments arranged by size: 2042 1122 962 861 838 648 359 159 120 MspA1I CmG'CkG Cuts at: 1606 4527 5204 6352 6597 1606 Size: 2921 677 1148 245 2120 Fragments arranged by size: 2921 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6559 NdeI CA'TA_TG Cuts at: 387 387 Size: 7111 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7111 NotI GC'GGCC_GC Cuts at: 4558 4558 Size: 7111 NspI r_CATG'y Cuts at: 1132 1229 2984 3905 4469 6014 1132 Size: 97 1755 921 564 1545 2229 Fragments arranged by size: 2229 1755 1545 921 564 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7111 PciI A'CATG_T Cuts at: 3901 6010 3901 Size: 2109 5002 PflMI CCAn_nnn'nTGG Cuts at: 1108 2801 1108 Size: 1693 5418 PfoI T'CCnGG_A Cuts at: 2056 2056 Size: 7111 PmeI GTTT'AAAC Cuts at: 4415 4415 Size: 7111 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5180 5212 6693 6725 5180 Size: 32 1481 32 5566 Fragments arranged by size: 5566 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4165 4165 Size: 7111 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7111 PsiI TTA'TAA Cuts at: 2442 4681 2442 Size: 2239 4872 PstI C_TGCA'G Cuts at: 830 4463 830 Size: 3633 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7111 SalI G'TCGA_C Cuts at: 1936 4453 1936 Size: 2517 4594 SapI GCTCTTCn'nnn_ Cuts at: 2793 2793 Size: 7111 SbfI CC_TGCA'GG Cuts at: 4463 4463 Size: 7111 ScaI AGT'ACT Cuts at: 5359 7071 5359 Size: 1712 5399 SfcI C'TryA_G Cuts at: 826 1045 3010 4459 5594 6275 6466 826 Size: 219 1965 1449 1135 681 191 1471 Fragments arranged by size: 1965 1471 1449 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7111 SmaI CCC'GGG Cuts at: 1872 4438 1872 Size: 2566 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2975 3375 3437 5215 6116 6378 Size: 197 926 1032 400 62 1778 901 262 Cuts at: 6378 820 Size: 1553 Fragments arranged by size: 1778 1553 1032 926 901 400 262 197 62 SnaBI TAC'GTA Cuts at: 493 4408 4902 493 Size: 3915 494 2702 Fragments arranged by size: 3915 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7111 SphI G_CATG'C Cuts at: 1132 4469 1132 Size: 3337 3774 SspI AAT'ATT Cuts at: 5 52 3005 5035 5 Size: 47 2953 2030 2081 Fragments arranged by size: 2953 2081 2030 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7111 StyI C'CwwG_G Cuts at: 513 1065 4550 513 Size: 552 3485 3074 Fragments arranged by size: 3485 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3018 5138 5155 5308 5493 5938 325 Size: 1255 1438 2120 17 153 185 445 1498 Fragments arranged by size: 2120 1498 1438 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3581 5357 7069 Size: 274 80 33 51 1443 1604 1776 1712 Cuts at: 7069 96 Size: 138 Fragments arranged by size: 1776 1712 1604 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2954 2986 7071 7103 2954 Size: 32 4085 32 2962 Fragments arranged by size: 4085 2962 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7111 XbaI T'CTAG_A Cuts at: 4447 4447 Size: 7111 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7111 XmaI C'CCGG_G Cuts at: 1870 4436 1870 Size: 2566 4545 XmnI GAAnn'nnTTC Cuts at: 4032 4371 5240 4032 Size: 339 869 5903 Fragments arranged by size: 5903 869 339 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4919 276 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI