(Circular) (Six-base) MAPSORT of: pFN21AB5479.seq Check: 1930 from: 1 to: 6187 pFN21AB5479 6187 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 2407 2966 3508 2407 Size: 559 542 5086 Fragments arranged by size: 5086 559 542 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2433 2564 2856 3243 4250 5634 5902 1632 Size: 801 131 292 387 1007 1384 268 1917 Fragments arranged by size: 1917 1384 1007 801 387 292 268 131 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 5086 6063 1203 Size: 3883 977 1327 Fragments arranged by size: 3883 1327 977 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 2616 1166 Size: 1450 4737 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2963 1184 Size: 1779 4408 AlwNI CAG_nnn'CTG Cuts at: 2470 2605 3930 5502 2470 Size: 135 1325 1572 3155 Fragments arranged by size: 3155 1572 1325 135 ApaLI G'TGCA_C Cuts at: 2609 4244 5400 2609 Size: 1635 1156 3396 Fragments arranged by size: 3396 1635 1156 ApoI r'AATT_y Cuts at: 1493 3496 3722 3874 5983 1493 Size: 2003 226 152 2109 1697 Fragments arranged by size: 2109 2003 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 3282 3512 1870 Size: 73 1339 230 4545 Fragments arranged by size: 4545 1339 230 73 BamHI G'GATC_C Cuts at: 3517 3884 3517 Size: 367 5820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2407 2425 2507 2966 3195 Size: 325 217 627 620 18 82 459 229 Cuts at: 3195 3299 3508 4963 618 Size: 104 209 1455 1842 Fragments arranged by size: 1842 1455 627 620 459 325 229 217 209 104 82 18 BanII G_rGCy'C Cuts at: 1391 2639 3506 1391 Size: 1248 867 4072 Fragments arranged by size: 4072 1248 867 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2844 3421 928 Size: 971 945 577 3694 Fragments arranged by size: 3694 971 945 577 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4378 4412 1903 Size: 34 2441 34 3678 Fragments arranged by size: 3678 2441 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2471 4079 5289 2471 Size: 1608 1210 3369 Fragments arranged by size: 3369 1608 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3085 4798 136 Size: 107 122 71 631 605 1413 1713 1525 Fragments arranged by size: 1713 1525 1413 631 605 122 107 71 BglII A'GATC_T Cuts at: 2769 6182 2769 Size: 3413 2774 BlpI GC'TnA_GC Cuts at: 3604 3604 Size: 6187 Bme1580I G_kGCm'C Cuts at: 1790 2613 2749 2767 3095 3304 3316 4248 5404 Size: 823 136 18 328 209 12 932 1156 Cuts at: 5404 1790 Size: 2573 Fragments arranged by size: 2573 1156 932 823 328 209 136 18 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 3918 4876 Size: 416 53 83 399 24 762 1739 958 Cuts at: 4876 442 Size: 1753 Fragments arranged by size: 1753 1739 958 762 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2334 2514 2334 Size: 180 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2146 2269 2312 2484 4847 1239 Size: 878 29 123 43 172 2363 2579 Fragments arranged by size: 2579 2363 878 172 123 43 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2681 3311 4312 5177 5475 2681 Size: 630 1001 865 298 3393 Fragments arranged by size: 3393 1001 865 630 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4850 882 Size: 617 3351 2219 Fragments arranged by size: 3351 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3484 3978 493 Size: 711 2280 494 2702 Fragments arranged by size: 2702 2280 711 494 BsaBI GATnn'nnATC Cuts at: 6181 6181 Size: 6187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3994 4376 5011 275 Size: 53 83 186 936 2461 382 635 1451 Fragments arranged by size: 2461 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2307 2988 4620 5018 5292 5439 5850 2307 Size: 681 1632 398 274 147 411 2644 Fragments arranged by size: 2644 1632 681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3068 3098 1178 Size: 30 165 30 1665 30 4267 Fragments arranged by size: 4267 1665 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3086 3089 1186 Size: 1900 3 4284 Fragments arranged by size: 4284 1900 3 BseYI C'CCAG_C Cuts at: 2026 2171 2397 5390 5886 2026 Size: 145 226 2993 496 2327 Fragments arranged by size: 2993 2327 496 226 145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3024 3251 3420 1163 Size: 573 1288 227 169 3930 Fragments arranged by size: 3930 1288 573 227 169 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3637 4398 5426 1540 Size: 275 186 1636 761 1028 2301 Fragments arranged by size: 2301 1636 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2613 2639 4248 4333 5404 2613 Size: 26 1609 85 1071 3396 Fragments arranged by size: 3396 1609 1071 85 26 BsmI GAATG_Cn' Cuts at: 2998 3698 3791 2998 Size: 700 93 5394 Fragments arranged by size: 5394 700 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2613 2639 2749 2767 3095 3304 3316 Size: 399 823 26 110 18 328 209 12 Cuts at: 3316 3506 4248 4333 5404 1391 Size: 190 742 85 1071 2174 Fragments arranged by size: 2174 1071 823 742 399 328 209 190 110 85 26 18 12 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 3033 4076 5806 3033 Size: 1043 1730 3414 Fragments arranged by size: 3414 1730 1043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2138 2850 3542 844 Size: 1294 712 692 3489 Fragments arranged by size: 3489 1294 712 692 BsrBI CCG'CTC Cuts at: 1394 1823 2388 4074 1394 Size: 429 565 1686 3507 Fragments arranged by size: 3507 1686 565 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2736 4682 4856 65 Size: 1183 1488 1946 174 1396 Fragments arranged by size: 1946 1488 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4831 5018 1700 Size: 110 3021 187 2869 Fragments arranged by size: 3021 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6187 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 4247 5259 4247 Size: 1012 5175 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6187 BstBI TT'CG_AA Cuts at: 2007 4991 2007 Size: 2984 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstXI CCAn_nnnn'nTGG Cuts at: 2056 2935 2941 3343 2056 Size: 879 6 402 4900 Fragments arranged by size: 4900 879 402 6 BstYI r'GATC_y Cuts at: 1972 2133 2247 2276 2769 3517 3884 4269 4286 Size: 161 114 29 493 748 367 385 17 Cuts at: 4286 5738 5824 5836 6182 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 748 493 385 367 346 161 114 86 29 17 12 BtgI C'CryG_G Cuts at: 513 1065 2846 2934 3342 513 Size: 552 1781 88 408 3358 Fragments arranged by size: 3358 1781 552 408 88 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2082 3136 487 Size: 683 56 856 1054 3538 Fragments arranged by size: 3538 1054 856 683 56 BtsI GCAGTG_nn' Cuts at: 2593 2915 3095 3703 4497 4517 2593 Size: 322 180 608 794 20 4263 Fragments arranged by size: 4263 794 608 322 180 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DraIII CAC_nnn'GTG Cuts at: 2187 2187 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 3124 3634 Size: 54 1425 50 275 18 216 1078 510 Cuts at: 3634 4523 6152 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1078 889 510 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3634 1537 Size: 275 1822 4090 Fragments arranged by size: 4090 1822 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2409 2429 4117 1353 Size: 1056 20 1688 3423 Fragments arranged by size: 3423 1688 1056 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4774 5148 5294 387 Size: 1064 138 3185 374 146 1280 Fragments arranged by size: 3185 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2637 2637 Size: 6187 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2146 2269 2312 2433 2484 2564 Size: 393 485 29 123 43 121 51 80 Cuts at: 2564 2856 3243 4250 4847 5634 5902 1239 Size: 292 387 1007 597 787 268 1524 Fragments arranged by size: 1524 1007 787 597 485 393 387 292 268 123 121 80 51 43 29 EcoNI CCTnn'n_nnAGG Cuts at: 2546 2546 Size: 6187 EcoO109I rG'GnC_Cy Cuts at: 2224 2419 3001 3359 2224 Size: 195 582 358 5052 Fragments arranged by size: 5052 582 358 195 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 FspI TGC'GCA Cuts at: 3184 4693 3184 Size: 1509 4678 HaeII r_GCGC'y Cuts at: 1678 2197 5334 1678 Size: 519 3137 2531 Fragments arranged by size: 3137 2531 519 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2775 2807 2813 2845 4828 Size: 32 135 32 1203 32 6 32 1983 Cuts at: 4828 4860 4902 4934 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1983 1203 135 42 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 2062 3531 3777 669 Size: 988 281 124 1469 246 3079 Fragments arranged by size: 3079 1469 988 281 246 124 HindIII A'AGCT_T Cuts at: 748 748 Size: 6187 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KpnI G_GTAC'C Cuts at: 2411 2970 3512 2411 Size: 559 542 5086 Fragments arranged by size: 5086 559 542 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5301 5485 1305 Size: 3996 184 2007 Fragments arranged by size: 3996 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 10 Size: 54 1425 343 216 4149 Fragments arranged by size: 4149 1425 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 2616 2618 2939 2945 4145 4504 Size: 648 1368 82 2 321 6 1200 359 Cuts at: 4504 4663 518 Size: 159 2042 Fragments arranged by size: 2042 1368 1200 648 359 321 159 82 6 2 MspA1I CmG'CkG Cuts at: 1606 2175 2470 3603 4280 5428 5673 1606 Size: 569 295 1133 677 1148 245 2120 Fragments arranged by size: 2120 1148 1133 677 569 295 245 NcoI C'CATG_G Cuts at: 513 1065 2934 3342 513 Size: 552 1869 408 3358 Fragments arranged by size: 3358 1869 552 408 NdeI CA'TA_TG Cuts at: 387 3474 387 Size: 3087 3100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 2044 2168 2533 3545 5090 1132 Size: 97 815 124 365 1012 1545 2229 Fragments arranged by size: 2229 1545 1012 815 365 124 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 2095 2356 2470 2605 1108 Size: 987 261 114 135 4690 Fragments arranged by size: 4690 987 261 135 114 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2224 2419 3001 3359 2224 Size: 195 582 358 5052 Fragments arranged by size: 5052 582 358 195 PshAI GACnn'nnGTC Cuts at: 1613 2091 2583 1613 Size: 478 492 5217 Fragments arranged by size: 5217 492 478 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PspXI vC'TCGA_Gb Cuts at: 3282 3282 Size: 6187 PstI C_TGCA'G Cuts at: 830 2149 3280 3539 830 Size: 1319 1131 259 3478 Fragments arranged by size: 3478 1319 1131 259 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 PvuII CAG'CTG Cuts at: 2470 2470 Size: 6187 SacI G_AGCT'C Cuts at: 2639 2639 Size: 6187 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SanDI GG'GwC_CC Cuts at: 3001 3001 Size: 6187 SapI GCTCTTCn'nnn_ Cuts at: 2429 2429 Size: 6187 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 2145 3276 3535 4670 5351 5542 826 Size: 219 1100 1131 259 1135 681 191 1471 Fragments arranged by size: 1471 1135 1131 1100 681 259 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3514 1872 Size: 1642 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2696 3282 3326 4291 5192 5454 Size: 197 926 753 586 44 965 901 262 Cuts at: 5454 820 Size: 1553 Fragments arranged by size: 1553 965 926 901 753 586 262 197 44 SnaBI TAC'GTA Cuts at: 493 3484 3978 493 Size: 2991 494 2702 Fragments arranged by size: 2991 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 3293 1517 Size: 1776 4411 StyI C'CwwG_G Cuts at: 513 1065 2640 2730 2928 2934 3342 3626 513 Size: 552 1575 90 198 6 408 284 3074 Fragments arranged by size: 3074 1575 552 408 284 198 90 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2198 4214 4231 4384 4569 5014 325 Size: 1255 618 2016 17 153 185 445 1498 Fragments arranged by size: 2016 1498 1255 618 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3065 4433 6145 Size: 274 80 33 51 1443 1088 1368 1712 Cuts at: 6145 96 Size: 138 Fragments arranged by size: 1712 1443 1368 1088 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3068 3100 6147 6179 3068 Size: 32 3047 32 3076 Fragments arranged by size: 3076 3047 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2501 2508 2501 Size: 7 6180 XhoI C'TCGA_G Cuts at: 1943 3282 1943 Size: 1339 4848 XmaI C'CCGG_G Cuts at: 1870 3512 1870 Size: 1642 4545 XmnI GAAnn'nnTTC Cuts at: 4316 4316 Size: 6187 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI