Restriction map for
pFN21ASDB2506
(Circular) (Six-base) MAPSORT of: pFN21ASDB2506.seq Check: 2560 from: 1 to: 5938
pFN21ASDB2506 5938 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3281 1937
Size: 1344 4594
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4001 5385 5653 1632
Size: 2369 1384 268 1917
Fragments arranged by size:
2369 1917 1384 268
AfeI AGC'GCT
Cuts at: 3127 3127
Size: 5938
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 2157 2949 3144 4837 5814 1203
Size: 954 792 195 1693 977 1327
Fragments arranged by size:
1693 1327 977 954 792 195
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 2096 1166
Size: 930 5008
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2832 2927 1184
Size: 1648 95 4195
Fragments arranged by size:
4195 1648 95
AlwNI CAG_nnn'CTG
Cuts at: 2862 3681 5253 2862
Size: 819 1572 3547
Fragments arranged by size:
3547 1572 819
ApaI G_GGCC'C
Cuts at: 2492 2492
Size: 5938
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 3247 3473 3625 5734 1493
Size: 1754 226 152 2109 1697
Fragments arranged by size:
2109 1754 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 2124 3263 1870
Size: 73 181 1139 4545
Fragments arranged by size:
4545 1139 181 73
BamHI G'GATC_C
Cuts at: 3268 3635 3268
Size: 367 5571
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2219 3259 4714 618
Size: 325 217 627 432 1040 1455 1842
Fragments arranged by size:
1842 1455 1040 627 432 325 217
BanII G_rGCy'C
Cuts at: 1391 2056 2297 2492 2613 3257 1391
Size: 665 241 195 121 644 4072
Fragments arranged by size:
4072 665 644 241 195 121
BbeI G_GCGC'C
Cuts at: 2223 2223
Size: 5938
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2860 3226 928
Size: 971 961 366 3640
Fragments arranged by size:
3640 971 961 366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3830 5040 3830
Size: 1210 4728
BclI T'GATC_A
Cuts at: 1548 2088 2226 1548
Size: 540 138 5260
Fragments arranged by size:
5260 540 138
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2301 2496 4549
Size: 107 122 71 631 605 629 195 2053
Cuts at: 4549 136
Size: 1525
Fragments arranged by size:
2053 1525 631 629 605 195 122 107
71
BglII A'GATC_T
Cuts at: 5933 5933
Size: 5938
BlpI GC'TnA_GC
Cuts at: 2444 3355 2444
Size: 911 5027
Bme1580I G_kGCm'C
Cuts at: 1790 2492 3999 5155 1790
Size: 702 1507 1156 2573
Fragments arranged by size:
2573 1507 1156 702
BmgBI CAC'GTC
Cuts at: 1318 2952 2992 1318
Size: 1634 40 4264
Fragments arranged by size:
4264 1634 40
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3093 3669 4627
Size: 416 53 83 399 24 1676 576 958
Cuts at: 4627 442
Size: 1753
Fragments arranged by size:
1753 1676 958 576 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4598 1239
Size: 3359 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4063 4928 5226 4063
Size: 865 298 4775
Fragments arranged by size:
4775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2372 4601 882
Size: 617 873 2229 2219
Fragments arranged by size:
2229 2219 873 617
BsaAI yAC'GTr
Cuts at: 493 1204 2158 3235 3729 493
Size: 711 954 1077 494 2702
Fragments arranged by size:
2702 1077 954 711 494
BsaBI GATnn'nnATC
Cuts at: 2246 5932 2246
Size: 3686 2252
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2178 2220 2412 3745
Size: 53 83 186 936 645 42 192 1333
Cuts at: 3745 4127 4762 275
Size: 382 635 1451
Fragments arranged by size:
1451 1333 936 645 635 382 192 186
83 53 42
BsaWI w'CCGG_w
Cuts at: 4371 4769 5043 5190 5601 4371
Size: 398 274 147 411 4708
Fragments arranged by size:
4708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5713
Fragments arranged by size:
5713 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2720 2999 1186
Size: 949 585 279 4125
Fragments arranged by size:
4125 949 585 279
BseYI C'CCAG_C
Cuts at: 2862 3122 5141 5637 2862
Size: 260 2019 496 3163
Fragments arranged by size:
3163 2019 496 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2887 3014 1163
Size: 573 1151 127 4087
Fragments arranged by size:
4087 1151 573 127
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3388 4149 5177 1540
Size: 275 186 1387 761 1028 2301
Fragments arranged by size:
2301 1387 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2056 2067 2571 2757 3177 3999 4084 5155 2056
Size: 11 504 186 420 822 85 1071 2839
Fragments arranged by size:
2839 1071 822 504 420 186 85 11
BsmI GAATG_Cn'
Cuts at: 3449 3542 3449
Size: 93 5845
BsmBI CGTCTCn'nnnn_
Cuts at: 2966 2966
Size: 5938
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2067 2297 2492 2571 2613 2757
Size: 399 266 11 230 195 79 42 144
Cuts at: 2757 3177 3257 3999 4084 5155 1391
Size: 420 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 420 399 266 230 195
144 85 80 79 42 11
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 3827 5557 3827
Size: 1730 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3293 844
Size: 2449 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3825 1394
Size: 429 2002 3507
Fragments arranged by size:
3507 2002 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2800 4433 4607 65
Size: 1183 1552 1633 174 1396
Fragments arranged by size:
1633 1552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4582 4769 1700
Size: 110 2772 187 2869
Fragments arranged by size:
2869 2772 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 2007 4742 2007
Size: 2735 3203
BstEII G'GTnAC_C
Cuts at: 1184 2659 1184
Size: 1475 4463
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2848 2029
Size: 819 5119
BstYI r'GATC_y
Cuts at: 1972 2895 3268 3635 4020 4037 5489 5575 5587
Size: 923 373 367 385 17 1452 86 12
Cuts at: 5587 5933 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 923 385 373 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2493 2613 3002 3033 513
Size: 552 1428 120 389 31 3418
Fragments arranged by size:
3418 1428 552 389 120 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2533 487
Size: 683 56 1307 3892
Fragments arranged by size:
3892 1307 683 56
BtsI GCAGTG_nn'
Cuts at: 2231 2899 3454 4248 4268 2231
Size: 668 555 794 20 3901
Fragments arranged by size:
3901 794 668 555 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DraIII CAC_nnn'GTG
Cuts at: 2062 2688 2062
Size: 626 5312
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2838 2853
Size: 54 1425 50 275 18 378 630 15
Cuts at: 2853 3165 3385 4274 5903 8
Size: 312 220 889 1629 43
Fragments arranged by size:
1629 1425 889 630 378 312 275 220
54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2285 2684 2792 3868 1353
Size: 734 198 399 108 1076 3423
Fragments arranged by size:
3423 1076 734 399 198 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2384 3192 4525 4899 5045 387
Size: 1064 138 795 808 1333 374 146 1280
Fragments arranged by size:
1333 1280 1064 808 795 374 146 138
EcoICRI GAG'CTC
Cuts at: 2054 2054
Size: 5938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4001 4598 5385 5653 1239
Size: 393 2369 597 787 268 1524
Fragments arranged by size:
2369 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2328 2489 2721 2328
Size: 161 232 5545
Fragments arranged by size:
5545 232 161
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 2223 2522 3129 5085 1678
Size: 545 299 607 1956 2531
Fragments arranged by size:
2531 1956 607 545 299
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2038 2070 4579 4611 4653
Size: 32 135 32 466 32 2509 32 42
Cuts at: 4653 4685 1373
Size: 32 2626
Fragments arranged by size:
2626 2509 466 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3282 3528 669
Size: 988 281 1344 246 3079
Fragments arranged by size:
3079 1344 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5938
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KasI G'GCGC_C
Cuts at: 2219 2219
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 3537 3537
Size: 5938
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5052 5236 1305
Size: 3747 184 2007
Fragments arranged by size:
3747 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2840 10
Size: 54 1425 343 1008 3108
Fragments arranged by size:
3108 1425 1008 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2096 2195 2552 2762 3896 4255
Size: 648 861 69 99 357 210 1134 359
Cuts at: 4255 4414 518
Size: 159 2042
Fragments arranged by size:
2042 1134 861 648 359 357 210 159
99 69
MspA1I CmG'CkG
Cuts at: 1606 2495 2506 2859 2998 3004 3354 4031 5179
Size: 889 11 353 139 6 350 677 1148
Cuts at: 5179 5424 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 889 677 353 350 245 139
11 6
NarI GG'CG_CC
Cuts at: 2220 2220
Size: 5938
NcoI C'CATG_G
Cuts at: 513 1065 2613 3033 513
Size: 552 1548 420 3418
Fragments arranged by size:
3418 1548 552 420
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 3296 4841 1132
Size: 97 2067 1545 2229
Fragments arranged by size:
2229 2067 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2332 2665 1348
Size: 984 333 4621
Fragments arranged by size:
4621 984 333
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2862 1108
Size: 1754 4184
PfoI T'CCnGG_A
Cuts at: 2367 2367
Size: 5938
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2328 2328
Size: 5938
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PspOMI G'GGCC_C
Cuts at: 2488 2488
Size: 5938
PstI C_TGCA'G
Cuts at: 830 2808 2925 3290 830
Size: 1978 117 365 3478
Fragments arranged by size:
3478 1978 365 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
PvuII CAG'CTG
Cuts at: 2506 2998 2506
Size: 492 5446
SacI G_AGCT'C
Cuts at: 2056 2056
Size: 5938
SacII CC_GC'GG
Cuts at: 2496 3005 2496
Size: 509 5429
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SanDI GG'GwC_CC
Cuts at: 2328 2328
Size: 5938
SapI GCTCTTCn'nnn_
Cuts at: 2087 2285 2087
Size: 198 5740
SbfI CC_TGCA'GG
Cuts at: 2925 3290 2925
Size: 365 5573
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SexAI A'CCwGG_T
Cuts at: 2058 2058
Size: 5938
SfcI C'TryA_G
Cuts at: 826 1045 2804 2921 3286 4421 5102 5293 826
Size: 219 1759 117 365 1135 681 191 1471
Fragments arranged by size:
1759 1471 1135 681 365 219 191 117
SfoI GGC'GCC
Cuts at: 2221 2221
Size: 5938
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4042 4943 5205 820
Size: 197 926 2099 901 262 1553
Fragments arranged by size:
2099 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3235 3729 493
Size: 2742 494 2702
Fragments arranged by size:
2742 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 3862 5
Size: 47 3810 2081
Fragments arranged by size:
3810 2081 47
StuI AGG'CCT
Cuts at: 1517 3026 1517
Size: 1509 4429
StyI C'CwwG_G
Cuts at: 513 1065 2613 2835 3033 3377 513
Size: 552 1548 222 198 344 3074
Fragments arranged by size:
3074 1548 552 344 222 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2752 3965 3982 4135 4320 4765 325
Size: 1255 1172 1213 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1213 1172 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4184 5896 96
Size: 274 80 33 51 1443 2207 1712 138
Fragments arranged by size:
2207 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5898 5930 5898
Size: 32 5906
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5938
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3082 3106 3082
Size: 24 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 4067 4067
Size: 5938
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SfiI SrfI SwaI