(Circular) (Six-base) MAPSORT of: pFN21ASDB1467.seq Check: 2489 from: 1 to: 9631 pFN21ASDB1467 9631 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3212 4096 3212 Size: 884 8747 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7441 278 Size: 53 83 186 936 5905 2468 Fragments arranged by size: 5905 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2217 3225 6974 1937 Size: 280 1008 3749 4594 Fragments arranged by size: 4594 3749 1008 280 Acc65I G'GTAC_C Cuts at: 6952 6952 Size: 9631 AclI AA'CG_TT Cuts at: 1563 7758 8131 1563 Size: 6195 373 3063 Fragments arranged by size: 6195 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2800 3176 3623 4233 4234 4667 4820 4916 Size: 1168 376 447 610 1 433 153 96 Cuts at: 4916 5651 7694 9078 9346 1632 Size: 735 2043 1384 268 1917 Fragments arranged by size: 2043 1917 1384 1168 735 610 447 433 376 268 153 96 1 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9434 AflIII A'CryG_T Cuts at: 1203 5646 8530 9507 1203 Size: 4443 2884 977 1327 Fragments arranged by size: 4443 2884 1327 977 AgeI A'CCGG_T Cuts at: 8462 8462 Size: 9631 AhdI GACnn_n'nnGTC Cuts at: 5640 8360 5640 Size: 2720 6911 AleI CACnn'nnGTG Cuts at: 1166 4135 1166 Size: 2969 6662 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2876 4043 5127 1184 Size: 1692 1167 1084 5688 Fragments arranged by size: 5688 1692 1167 1084 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4904 4936 4904 Size: 32 9599 AlwNI CAG_nnn'CTG Cuts at: 2787 3247 3532 4261 4760 4798 4942 5181 7374 Size: 460 285 729 499 38 144 239 2193 Cuts at: 7374 8946 2787 Size: 1572 3472 Fragments arranged by size: 3472 2193 1572 729 499 460 285 239 144 38 ApaI G_GGCC'C Cuts at: 6517 6517 Size: 9631 ApaLI G'TGCA_C Cuts at: 4329 7688 8844 4329 Size: 3359 1156 5116 Fragments arranged by size: 5116 3359 1156 ApoI r'AATT_y Cuts at: 1493 3726 6651 6940 7166 7318 9427 1493 Size: 2233 2925 289 226 152 2109 1697 Fragments arranged by size: 2925 2233 2109 1697 289 226 152 AseI AT'TA_AT Cuts at: 160 1032 8185 160 Size: 872 7153 1606 Fragments arranged by size: 7153 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9631 AvaI C'yCGr_G Cuts at: 1870 1943 2505 3137 3860 4122 6333 6456 6956 Size: 73 562 632 723 262 2211 123 500 Cuts at: 6956 1870 Size: 4545 Fragments arranged by size: 4545 2211 723 632 562 500 262 123 73 BamHI G'GATC_C Cuts at: 2640 3736 5236 5880 6753 6961 7328 2640 Size: 1096 1500 644 873 208 367 4943 Fragments arranged by size: 4943 1500 1096 873 644 367 208 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2682 2872 4207 6476 6917 Size: 325 217 627 895 190 1335 2269 441 Cuts at: 6917 6952 8407 618 Size: 35 1455 1842 Fragments arranged by size: 2269 1842 1455 1335 895 627 441 325 217 190 35 BanII G_rGCy'C Cuts at: 1391 2471 2963 3464 4045 4181 4577 4689 6059 Size: 1080 492 501 581 136 396 112 1370 Cuts at: 6059 6113 6517 6786 6950 1391 Size: 54 404 269 164 4072 Fragments arranged by size: 4072 1370 1080 581 501 492 404 396 269 164 136 112 54 BbeI G_GCGC'C Cuts at: 2686 2686 Size: 9631 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2019 3530 4470 5639 5719 6887 928 Size: 971 120 1511 940 1169 80 1168 3672 Fragments arranged by size: 3672 1511 1169 1168 971 940 120 80 BbvCI CC'TCA_GC Cuts at: 3995 3995 Size: 9631 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4539 4573 5297 5331 7822 7856 1903 Size: 34 2602 34 724 34 2491 34 3678 Fragments arranged by size: 3678 2602 2491 724 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2515 5935 7523 8733 2515 Size: 3420 1588 1210 3413 Fragments arranged by size: 3420 3413 1588 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9631 BfrBI ATG'CAT Cuts at: 6595 6595 Size: 9631 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3310 4005 5030 Size: 107 122 71 631 605 1638 695 1025 Cuts at: 5030 8242 136 Size: 3212 1525 Fragments arranged by size: 3212 1638 1525 1025 695 631 605 122 107 71 BglII A'GATC_T Cuts at: 2568 4089 5331 6153 9626 2568 Size: 1521 1242 822 3473 2573 Fragments arranged by size: 3473 2573 1521 1242 822 BlpI GC'TnA_GC Cuts at: 4761 6791 7048 4761 Size: 2030 257 7344 Fragments arranged by size: 7344 2030 257 Bme1580I G_kGCm'C Cuts at: 1790 2877 3861 4333 5175 5719 6479 6517 7692 Size: 1087 984 472 842 544 760 38 1175 Cuts at: 7692 8848 1790 Size: 1156 2573 Fragments arranged by size: 2573 1175 1156 1087 984 842 760 544 472 38 BmgBI CAC'GTC Cuts at: 1318 3283 1318 Size: 1965 7666 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2574 2699 3735 Size: 416 53 83 399 24 1157 125 1036 Cuts at: 3735 4094 5142 7362 8320 442 Size: 359 1048 2220 958 1753 Fragments arranged by size: 2220 1753 1157 1048 1036 958 416 399 359 125 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2096 1056 Size: 1040 8591 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2420 4591 4890 2420 Size: 2171 299 7161 Fragments arranged by size: 7161 2171 299 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2415 3127 3335 4437 5111 5921 6602 Size: 845 331 712 208 1102 674 810 681 Cuts at: 6602 6770 8291 1239 Size: 168 1521 2579 Fragments arranged by size: 2579 1521 1102 845 810 712 681 674 331 208 168 Bpu10I CC'TnA_GC Cuts at: 3995 6873 3995 Size: 2878 6753 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2240 4885 4892 5894 6089 7756 8621 8919 2240 Size: 2645 7 1002 195 1667 865 298 2952 Fragments arranged by size: 2952 2645 1667 1002 865 298 195 7 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2276 3010 5423 8294 882 Size: 617 777 734 2413 2871 2219 Fragments arranged by size: 2871 2413 2219 777 734 617 BsaAI yAC'GTr Cuts at: 493 1204 5586 6928 7422 493 Size: 711 4382 1342 494 2702 Fragments arranged by size: 4382 2702 1342 711 494 BsaBI GATnn'nnATC Cuts at: 5318 9625 5318 Size: 4307 5324 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2683 2921 7438 7820 Size: 53 83 186 936 1150 238 4517 382 Cuts at: 7820 8455 275 Size: 635 1451 Fragments arranged by size: 4517 1451 1150 936 635 382 238 186 83 53 BsaWI w'CCGG_w Cuts at: 8064 8462 8736 8883 9294 8064 Size: 398 274 147 411 8401 Fragments arranged by size: 8401 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2327 2357 3200 3230 5180 Size: 30 165 30 924 30 843 30 1950 Cuts at: 5180 5210 5513 5543 6330 6360 6528 6558 1178 Size: 30 303 30 787 30 168 30 4251 Fragments arranged by size: 4251 1950 924 843 787 303 168 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 2484 3038 3332 4171 5108 6257 6380 Size: 985 313 554 294 839 937 1149 123 Cuts at: 6380 6821 1186 Size: 441 3996 Fragments arranged by size: 3996 1149 985 937 839 554 441 313 294 123 BseYI C'CCAG_C Cuts at: 2199 2574 2662 4224 4313 4604 4608 4782 5034 Size: 375 88 1562 89 291 4 174 252 Cuts at: 5034 6299 6484 8834 9330 2199 Size: 1265 185 2350 496 2500 Fragments arranged by size: 2500 2350 1562 1265 496 375 291 252 185 174 89 88 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4039 4124 4754 1163 Size: 573 2303 85 630 6040 Fragments arranged by size: 6040 2303 630 573 85 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5392 6328 7081 7842 8870 1540 Size: 275 186 3391 936 753 761 1028 2301 Fragments arranged by size: 3391 2301 1028 936 761 753 275 186 BsiHKAI G_wGCw'C Cuts at: 3135 3685 4045 4181 4333 4577 4593 6113 6786 Size: 550 360 136 152 244 16 1520 673 Cuts at: 6786 7692 7777 8848 3135 Size: 906 85 1071 3918 Fragments arranged by size: 3918 1520 1071 906 673 550 360 244 152 136 85 16 BsiWI C'GTAC_G Cuts at: 5586 5586 Size: 9631 BsmI GAATG_Cn' Cuts at: 2920 4814 7142 7235 2920 Size: 1894 2328 93 5316 Fragments arranged by size: 5316 2328 1894 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2471 2877 2963 3135 3464 3685 3861 Size: 399 681 406 86 172 329 221 176 Cuts at: 3861 4045 4181 4333 4577 4593 4689 5175 5719 Size: 184 136 152 244 16 96 486 544 Cuts at: 5719 6059 6113 6479 6517 6786 6950 7692 7777 Size: 340 54 366 38 269 164 742 85 Cuts at: 7777 8848 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 681 544 486 406 399 366 340 329 269 244 221 184 176 172 164 152 136 96 86 85 54 38 16 BspEI T'CCGG_A Cuts at: 9294 9294 Size: 9631 BspHI T'CATG_A Cuts at: 3352 5322 7520 9250 3352 Size: 1970 2198 1730 3733 Fragments arranged by size: 3733 2198 1970 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3212 4096 4246 4785 5029 6722 6777 6986 Size: 2368 884 150 539 244 1693 55 209 Cuts at: 6986 844 Size: 3489 Fragments arranged by size: 3489 2368 1693 884 539 244 209 150 55 BsrBI CCG'CTC Cuts at: 1394 1823 6330 6355 7518 1394 Size: 429 4507 25 1163 3507 Fragments arranged by size: 4507 3507 1163 429 25 BsrDI GCAATG_nn' Cuts at: 65 1248 2047 8126 8300 65 Size: 1183 799 6079 174 1396 Fragments arranged by size: 6079 1396 1183 799 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4113 5387 5465 5481 6066 6884 8275 Size: 110 2303 1274 78 16 585 818 1391 Cuts at: 8275 8462 1700 Size: 187 2869 Fragments arranged by size: 2869 2303 1391 1274 818 585 187 110 78 16 BsrGI T'GTAC_A Cuts at: 96 6432 96 Size: 6336 3295 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9631 BssSI C'ACGA_G Cuts at: 5125 6212 7691 8703 5125 Size: 1087 1479 1012 6053 Fragments arranged by size: 6053 1479 1087 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9631 BstBI TT'CG_AA Cuts at: 2007 8435 2007 Size: 6428 3203 BstEII G'GTnAC_C Cuts at: 1184 5461 6764 1184 Size: 4277 1303 4051 Fragments arranged by size: 4277 4051 1303 BstXI CCAn_nnnn'nTGG Cuts at: 3748 3913 5155 5693 3748 Size: 165 1242 538 7686 Fragments arranged by size: 7686 1242 538 165 BstYI r'GATC_y Cuts at: 1972 2368 2568 2640 3736 4089 5236 5331 5442 Size: 396 200 72 1096 353 1147 95 111 Cuts at: 5442 5880 6153 6753 6902 6961 7328 7713 7730 Size: 438 273 600 149 59 367 385 17 Cuts at: 7730 9182 9268 9280 9626 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1147 1096 600 438 396 385 367 353 346 273 200 149 111 95 86 72 59 17 12 Bsu36I CC'TnA_GG Cuts at: 2868 3269 5930 5937 6408 2868 Size: 401 2661 7 471 6091 Fragments arranged by size: 6091 2661 471 401 7 BtgI C'CryG_G Cuts at: 513 1065 2841 3657 4000 513 Size: 552 1776 816 343 6144 Fragments arranged by size: 6144 1776 816 552 343 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2111 487 Size: 683 56 885 8007 Fragments arranged by size: 8007 885 683 56 BtsI GCAGTG_nn' Cuts at: 3433 3861 4130 4658 4742 6004 6449 7147 7941 Size: 428 269 528 84 1262 445 698 794 Cuts at: 7941 7961 3433 Size: 20 5103 Fragments arranged by size: 5103 1262 794 698 528 445 428 269 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9631 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9596 DraI TTT'AAA Cuts at: 6935 6935 Size: 9631 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3617 8638 809 Size: 507 2301 5021 1802 Fragments arranged by size: 5021 2301 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2844 2986 Size: 54 1425 50 275 18 270 744 142 Cuts at: 2986 3651 4270 4292 5022 5389 5580 5665 6115 Size: 665 619 22 730 367 191 85 450 Cuts at: 6115 6325 6348 6414 7078 7967 9596 8 Size: 210 23 66 664 889 1629 43 Fragments arranged by size: 1629 1425 889 744 730 665 664 619 450 367 275 270 210 191 142 85 66 54 50 43 23 22 18 EagI C'GGCC_G Cuts at: 1537 1812 5389 6325 7078 1537 Size: 275 3577 936 753 4090 Fragments arranged by size: 4090 3577 936 753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2443 2643 2649 3006 4033 4067 4247 4980 Size: 1090 200 6 357 1027 34 180 733 Cuts at: 4980 5849 6284 6584 7561 1353 Size: 869 435 300 977 3423 Fragments arranged by size: 3423 1090 1027 977 869 733 435 357 300 200 180 34 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2260 6373 6839 8218 8592 8738 Size: 1064 138 671 4113 466 1379 374 146 Cuts at: 8738 387 Size: 1280 Fragments arranged by size: 4113 1379 1280 1064 671 466 374 146 138 EcoICRI GAG'CTC Cuts at: 4043 4179 4575 6111 6784 4043 Size: 136 396 1536 673 6890 Fragments arranged by size: 6890 1536 673 396 136 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2415 2800 3127 3176 3335 3623 Size: 393 452 331 385 327 49 159 288 Cuts at: 3623 4233 4234 4437 4667 4820 4916 5111 5651 Size: 610 1 203 230 153 96 195 540 Cuts at: 5651 5921 6602 6770 7694 8291 9078 9346 1239 Size: 270 681 168 924 597 787 268 1524 Fragments arranged by size: 1524 924 787 681 610 597 540 452 393 385 331 327 288 270 268 230 203 195 168 159 153 96 49 1 EcoNI CCTnn'n_nnAGG Cuts at: 2648 3884 5093 5288 6836 6872 2648 Size: 1236 1209 195 1548 36 5407 Fragments arranged by size: 5407 1548 1236 1209 195 36 EcoO109I rG'GnC_Cy Cuts at: 2550 3529 3909 4258 4795 5905 2550 Size: 979 380 349 537 1110 6276 Fragments arranged by size: 6276 1110 979 537 380 349 EcoRI G'AATT_C Cuts at: 6940 7318 6940 Size: 378 9253 EcoRV GAT'ATC Cuts at: 3055 3055 Size: 9631 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3358 4078 5125 3358 Size: 720 1047 7864 Fragments arranged by size: 7864 1047 720 FspI TGC'GCA Cuts at: 3425 5360 8137 3425 Size: 1935 2777 4919 Fragments arranged by size: 4919 2777 1935 FspAI rTGC'GCAy Cuts at: 5360 5360 Size: 9631 HaeII r_GCGC'y Cuts at: 1678 2686 4320 4537 4602 8778 1678 Size: 1008 1634 217 65 4176 2531 Fragments arranged by size: 4176 2531 1634 1008 217 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 2499 2531 3759 Size: 32 135 32 545 32 350 32 1228 Cuts at: 3759 3791 5626 5658 8272 8304 8346 8378 1373 Size: 32 1835 32 2614 32 42 32 2626 Fragments arranged by size: 2626 2614 1835 1228 545 350 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3151 5797 6975 7221 669 Size: 988 281 1213 2646 1178 246 3079 Fragments arranged by size: 3079 2646 1213 1178 988 281 246 HindIII A'AGCT_T Cuts at: 748 4277 4475 748 Size: 3529 198 5904 Fragments arranged by size: 5904 3529 198 HpaI GTT'AAC Cuts at: 7221 7221 Size: 9631 KasI G'GCGC_C Cuts at: 2682 2682 Size: 9631 KpnI G_GTAC'C Cuts at: 6956 6956 Size: 9631 MfeI C'AATT_G Cuts at: 2612 7230 2612 Size: 4618 5013 MluI A'CGCG_T Cuts at: 9507 9507 Size: 9631 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4304 6505 8745 8929 1305 Size: 2999 2201 2240 184 2007 Fragments arranged by size: 2999 2240 2201 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2846 2988 4294 5024 5582 Size: 54 1425 343 1014 142 1306 730 558 Cuts at: 5582 10 Size: 4059 Fragments arranged by size: 4059 1425 1306 1014 730 558 343 142 54 MslI CAynn'nnrTG Cuts at: 518 1166 4135 4674 5360 6587 6590 7589 7948 Size: 648 2969 539 686 1227 3 999 359 Cuts at: 7948 8107 518 Size: 159 2042 Fragments arranged by size: 2969 2042 1227 999 686 648 539 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2199 2809 3403 3694 3794 4201 4608 4786 Size: 593 610 594 291 100 407 407 178 Cuts at: 4786 5376 6232 6778 6790 7047 7724 8872 9117 Size: 590 856 546 12 257 677 1148 245 Cuts at: 9117 1606 Size: 2120 Fragments arranged by size: 2120 1148 856 677 610 594 593 590 546 407 407 291 257 245 178 100 12 NarI GG'CG_CC Cuts at: 2683 2683 Size: 9631 NcoI C'CATG_G Cuts at: 513 1065 4000 513 Size: 552 2935 6144 Fragments arranged by size: 6144 2935 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9631 NheI G'CTAG_C Cuts at: 1052 2092 1052 Size: 1040 8591 NotI GC'GGCC_GC Cuts at: 7078 7078 Size: 9631 NsiI A_TGCA'T Cuts at: 6597 6597 Size: 9631 NspI r_CATG'y Cuts at: 1132 1229 3049 3341 3718 4697 5299 5359 5650 Size: 97 1820 292 377 979 602 60 291 Cuts at: 5650 6989 8534 1132 Size: 1339 1545 2229 Fragments arranged by size: 2229 1820 1545 1339 979 602 377 292 291 97 60 PasI CC'CwG_GG Cuts at: 1348 4219 4681 5175 5242 5516 6898 1348 Size: 2871 462 494 67 274 1382 4081 Fragments arranged by size: 4081 2871 1382 494 462 274 67 PciI A'CATG_T Cuts at: 5646 8530 5646 Size: 2884 6747 PflMI CCAn_nnn'nTGG Cuts at: 1108 2360 2787 3049 3954 5233 6034 1108 Size: 1252 427 262 905 1279 801 4705 Fragments arranged by size: 4705 1279 1252 905 801 427 262 PfoI T'CCnGG_A Cuts at: 3885 4455 4704 5043 5340 6249 6618 3885 Size: 570 249 339 297 909 369 6898 Fragments arranged by size: 6898 909 570 369 339 297 249 PmeI GTTT'AAAC Cuts at: 6935 6935 Size: 9631 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6085 6117 7700 7732 9213 9245 6085 Size: 32 1583 32 1481 32 6471 Fragments arranged by size: 6471 1583 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2550 3909 4258 5905 2550 Size: 1359 349 1647 6276 Fragments arranged by size: 6276 1647 1359 349 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9631 PsiI TTA'TAA Cuts at: 7201 7201 Size: 9631 PspOMI G'GGCC_C Cuts at: 6513 6513 Size: 9631 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5540 5572 5540 Size: 32 9599 PstI C_TGCA'G Cuts at: 830 2115 2950 3330 4032 4368 5310 5505 6774 Size: 1285 835 380 702 336 942 195 1269 Cuts at: 6774 6983 830 Size: 209 3478 Fragments arranged by size: 3478 1285 1269 942 835 702 380 336 209 195 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9631 PvuII CAG'CTG Cuts at: 2199 3403 3694 4608 4786 6790 2199 Size: 1204 291 914 178 2004 5040 Fragments arranged by size: 5040 2004 1204 914 291 178 RsrII CG'GwC_CG Cuts at: 2247 2247 Size: 9631 SacI G_AGCT'C Cuts at: 4045 4181 4577 6113 6786 4045 Size: 136 396 1536 673 6890 Fragments arranged by size: 6890 1536 673 396 136 SalI G'TCGA_C Cuts at: 1936 6973 1936 Size: 5037 4594 SanDI GG'GwC_CC Cuts at: 2550 2550 Size: 9631 SapI GCTCTTCn'nnn_ Cuts at: 4033 6284 4033 Size: 2251 7380 SbfI CC_TGCA'GG Cuts at: 6983 6983 Size: 9631 ScaI AGT'ACT Cuts at: 7879 9591 7879 Size: 1712 7919 SexAI A'CCwGG_T Cuts at: 3807 3879 3807 Size: 72 9559 SfcI C'TryA_G Cuts at: 826 1045 2111 2946 3222 3326 3398 4028 4364 Size: 219 1066 835 276 104 72 630 336 Cuts at: 4364 5306 5501 6770 6979 8114 8795 8986 826 Size: 942 195 1269 209 1135 681 191 1471 Fragments arranged by size: 1471 1269 1135 1066 942 835 681 630 336 276 219 209 195 191 104 72 SfiI GGCCn_nnn'nGGCC Cuts at: 5030 5030 Size: 9631 SfoI GGC'GCC Cuts at: 2684 2684 Size: 9631 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9631 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9631 SmaI CCC'GGG Cuts at: 1872 3862 6335 6458 6958 1872 Size: 1990 2473 123 500 4545 Fragments arranged by size: 4545 2473 1990 500 123 SmlI C'TyrA_G Cuts at: 820 1017 1943 2255 4864 4907 5909 6104 7735 Size: 197 926 312 2609 43 1002 195 1631 Cuts at: 7735 8636 8898 820 Size: 901 262 1553 Fragments arranged by size: 2609 1631 1553 1002 926 901 312 262 197 195 43 SnaBI TAC'GTA Cuts at: 493 6928 7422 493 Size: 6435 494 2702 Fragments arranged by size: 6435 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9631 SphI G_CATG'C Cuts at: 1132 3341 3718 4697 6989 1132 Size: 2209 377 979 2292 3774 Fragments arranged by size: 3774 2292 2209 979 377 SspI AAT'ATT Cuts at: 5 52 7555 5 Size: 47 7503 2081 Fragments arranged by size: 7503 2081 47 StuI AGG'CCT Cuts at: 1517 3502 3893 5935 1517 Size: 1985 391 2042 5213 Fragments arranged by size: 5213 2042 1985 391 StyI C'CwwG_G Cuts at: 513 1065 2347 3342 4000 6759 7070 513 Size: 552 1282 995 658 2759 311 3074 Fragments arranged by size: 3074 2759 1282 995 658 552 311 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2264 3272 3275 3950 5234 5622 5852 Size: 1255 684 1008 3 675 1284 388 230 Cuts at: 5852 6462 7658 7675 7828 8013 8458 325 Size: 610 1196 17 153 185 445 1498 Fragments arranged by size: 1498 1284 1255 1196 1008 684 675 610 445 388 230 185 153 17 3 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3357 3819 4506 Size: 274 80 33 51 1443 1380 462 687 Cuts at: 4506 6432 7877 9589 96 Size: 1926 1445 1712 138 Fragments arranged by size: 1926 1712 1445 1443 1380 687 462 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 2327 2359 2826 2858 4323 4355 6330 Size: 32 253 32 467 32 1465 32 1975 Cuts at: 6330 6362 6528 6560 9591 9623 2042 Size: 32 166 32 3031 32 2050 Fragments arranged by size: 3031 2050 1975 1465 467 253 166 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3881 4355 1316 Size: 2565 474 6592 Fragments arranged by size: 6592 2565 474 XbaI T'CTAG_A Cuts at: 6967 6967 Size: 9631 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3805 5453 5590 5660 3805 Size: 1648 137 70 7776 Fragments arranged by size: 7776 1648 137 70 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9631 XmaI C'CCGG_G Cuts at: 1870 3860 6333 6456 6956 1870 Size: 1990 2473 123 500 4545 Fragments arranged by size: 4545 2473 1990 500 123 XmnI GAAnn'nnTTC Cuts at: 5105 6155 7760 5105 Size: 1050 1605 6976 Fragments arranged by size: 6976 1605 1050 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7439 276 Size: 53 83 186 936 5905 2468 Fragments arranged by size: 5905 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BsmBI BstZ17I DraIII FseI NaeI NgoMIV NruI PacI PmlI PspXI SacII SrfI SwaI