Restriction map for
pFN21ASDB1453
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccI AccII AclI AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BciVI BglI
BglII BlnI BlpI Bpu1102I BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRV EcoT22I FauI FseI FspI
HgaI HindIII HpaI KasI KpnI
KspI MfeI MluI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AatII 824 GACGTC GACGT^C
AccIII 191 TCCGGA T^CCGGA
AciI 406 GCGG CCGC(-3/-1)
AcyI 824 GACGTC G[GA]^CG[CT]C
AflII 1255 CTTAAG C^TTAAG
AlwI 1062 GGATC GGATC(4/5)
AlwNI 1241 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 871 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1337 AGCGCT AGC^GCT
Asp700I 450 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1106 GAGCTC G[AT]GC[AT]^C
AspI 1309 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1106 GAGCTC G[GA]GC[CT]^C
BbsI 1315 GTCTTC GAAGAC(2/6)
BcgI 95 GCAACCTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 929 TGATCA T^GATCA
BcnI 890 CCGGG CC^[GC]GG
BfrI 1255 CTTAAG C^TTAAG
BmyI 1106 GAGCTC G[AGT]GC[ACT]^C
BpuAI 1315 GTCTTC GAAGAC(2/6)
BsaAI 1329 CACGTA [CT]AC^GT[GA]
BsaBI 942 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 824 GACGTC G[GA]^CG[CT]C
BsaJI 1058 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 191 TCCGGA [AT]^CCGG[AT]
BseAI 191 TCCGGA T^CCGGA
BsiHKAI 1106 GAGCTC G[AT]GC[AT]^C
Bsp1286I 1106 GAGCTC G[AGT]GC[ACT]^C
BspEI 191 TCCGGA T^CCGGA
BspLU11I 336 ACATGT A^CATGT
BstYI 1062 GGATCT [GA]^GATC[CT]
Bsu36I 871 CCTGAGG CC^T[ACGT]AGG
Cac8I 831 GCTAGC GC[ACGT]^[ACGT]GC
CfoI 1338 GCGC GCG^C
DraII 1346 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eco47III 1337 AGCGCT AGC^GCT
Eco81I 871 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1346 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 786 GAATTC G^AATTC
EcoT14I 1058 CCAAGG C^C[AT][AT]GG
FbaI 929 TGATCA T^GATCA
HaeII 1337 AGCGCT [GA]GCGC^[CT]
HhaI 1338 GCGC GCG^C
Hin1I 824 GACGTC G[GA]^CG[CT]C
HinP1I 1338 GCGC G^CGC
HincII 1153 GTTGAC GT[CT]^[GA]AC
HindII 1153 GTTGAC GT[CT]^[GA]AC
MamI 942 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1062 GGATCT [GA]^GATC[CT]
MroI 191 TCCGGA T^CCGGA
NciI 890 CCGGG CC^[GC]GG
NheI 831 GCTAGC G^CTAGC
PflFI 1309 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 688 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1106 GAGCTC GAGCT^C
SfaNI 942 GATGC GCATC(5/9)
SfcI 459 CTATAG C^T[GA][CT]AG
StuI 165 AGGCCT AGG^CCT
StyI 1058 CCAAGG C^C[AT][AT]GG
TaqI 1229 TCGA T^CGA
Tth111I 1309 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1229 TCGA T^CGA
XcmI 68 CCAAAGTGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1062 GGATCT [GA]^GATC[CT]
XmnI 450 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 336 ACATGT A^C[GA][CT]GT
1328 ACACGT
BalI 393 TGGCCA TGG^CCA
1268 TGGCCA
BbvI 1387 GCAGC GCAGC(8/12)
1183 GCTGC
BfaI 421 CTAG C^TAG
832 CTAG
BsaI 973 GAGACC GGTCTC(1/5)
1176 GAGACC
BsmFI 333 GGGAC GGGAC(10/14)
515 GGGAC
BspMI 1179 ACCTGC ACCTGC(4/8)
387 GCAGGT
Cfr13I 669 GGGCC G^G[ACGT]CC
1347 GGGCC
EaeI 393 TGGCCA [CT]^GGCC[GA]
1268 TGGCCA
EarI 237 GAAGAG CTCTTC(1/4)
556 GAAGAG
Fnu4HI 1183 GCTGC GC^[ACGT]GC
1387 GCAGC
HapII 192 CCGG C^CGG
890 CCGG
HpaII 192 CCGG C^CGG
890 CCGG
ItaI 1183 GCTGC GC^[ACGT]GC
1387 GCAGC
Ksp632I 237 GAAGAG CTCTTC(1/4)
556 GAAGAG
MaeI 421 CTAG C^TAG
832 CTAG
MaeII 825 ACGT A^CGT
1330 ACGT
MluNI 393 TGGCCA TGG^CCA
1268 TGGCCA
MscI 393 TGGCCA TGG^CCA
1268 TGGCCA
MseI 274 TTAA T^TAA
1256 TTAA
MspI 192 CCGG C^CGG
890 CCGG
MwoI 1340 GCTTTGAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1357 GCAAGGGGGGC
NspI 204 ACATGC [GA]CATG^[CT]
336 ACATGT
Sau96I 669 GGGCC G^G[ACGT]CC
1347 GGGCC
SmlI 980 CTCAAG C^T[CT][GA]AG
1255 CTTAAG
Tru9I 274 TTAA T^TAA
1256 TTAA
TseI 1183 GCTGC G^C[AT]GC
1387 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AfaI AluI ApoI BpmI
BseRI BsiYI BslI BsmAI BsrDI
BsrI BssKI BstF5I BstNI DdeI
DpnII EcoRII FokI HaeIII HinfI
HphI MaeIII MboI MboII MnlI
MslI MvaI NdeII NlaIII PleI
RsaI Sau3AI ScrFI TfiI Tsp45I
Tsp509I TspRI