(Circular) (Six-base) MAPSORT of: pFN21ASDB1453.seq Check: 8292 from: 1 to: 6124 pFN21ASDB1453 6124 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2851 3934 278 Size: 53 83 186 936 1315 1083 2468 Fragments arranged by size: 2468 1315 1083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3467 1937 Size: 1530 4594 Acc65I G'GTAC_C Cuts at: 3445 3445 Size: 6124 AclI AA'CG_TT Cuts at: 1563 4251 4624 1563 Size: 2688 373 3063 Fragments arranged by size: 3063 2688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4187 5571 5839 1632 Size: 2555 1384 268 1917 Fragments arranged by size: 2555 1917 1384 268 AfeI AGC'GCT Cuts at: 3362 3362 Size: 6124 AflII C'TTAA_G Cuts at: 820 1017 3278 820 Size: 197 2261 3666 Fragments arranged by size: 3666 2261 197 AflIII A'CryG_T Cuts at: 1203 2359 3351 5023 6000 1203 Size: 1156 992 1672 977 1327 Fragments arranged by size: 1672 1327 1156 992 977 AgeI A'CCGG_T Cuts at: 4955 4955 Size: 6124 AhdI GACnn_n'nnGTC Cuts at: 4853 4853 Size: 6124 AleI CACnn'nnGTG Cuts at: 1166 2424 1166 Size: 1258 4866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6124 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2795 2827 2795 Size: 32 6092 AlwNI CAG_nnn'CTG Cuts at: 3269 3867 5439 3269 Size: 598 1572 3954 Fragments arranged by size: 3954 1572 598 ApaLI G'TGCA_C Cuts at: 4181 5337 4181 Size: 1156 4968 ApoI r'AATT_y Cuts at: 1493 2107 2753 2809 3433 3659 3811 5920 1493 Size: 614 646 56 624 226 152 2109 1697 Fragments arranged by size: 2109 1697 646 624 614 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 4678 160 Size: 872 3646 1606 Fragments arranged by size: 3646 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 3449 1870 Size: 73 1506 4545 Fragments arranged by size: 4545 1506 73 BamHI G'GATC_C Cuts at: 3454 3821 3454 Size: 367 5757 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3445 4900 618 Size: 325 217 627 1658 1455 1842 Fragments arranged by size: 1842 1658 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3133 3443 1391 Size: 1742 310 4072 Fragments arranged by size: 4072 1742 310 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3331 928 Size: 971 1432 3721 Fragments arranged by size: 3721 1432 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2107 2141 4315 4349 1903 Size: 34 170 34 2174 34 3678 Fragments arranged by size: 3678 2174 170 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4016 5226 4016 Size: 1210 4914 BclI T'GATC_A Cuts at: 1548 2952 1548 Size: 1404 4720 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4735 136 Size: 107 122 71 631 605 3063 1525 Fragments arranged by size: 3063 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6119 6119 Size: 6124 BlpI GC'TnA_GC Cuts at: 3541 3541 Size: 6124 Bme1580I G_kGCm'C Cuts at: 1790 4185 5341 1790 Size: 2395 1156 2573 Fragments arranged by size: 2573 2395 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2778 3190 3343 Size: 416 53 83 399 24 1361 412 153 Cuts at: 3343 3855 4813 442 Size: 512 958 1753 Fragments arranged by size: 1753 1361 958 512 416 412 399 153 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2858 1056 Size: 1802 4322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2553 3039 3156 4784 1239 Size: 884 430 486 117 1628 2579 Fragments arranged by size: 2579 1628 884 486 430 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2988 4249 5114 5412 2988 Size: 1261 865 298 3700 Fragments arranged by size: 3700 1261 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2990 3193 4787 882 Size: 617 1491 203 1594 2219 Fragments arranged by size: 2219 1594 1491 617 203 BsaAI yAC'GTr Cuts at: 493 1204 3354 3421 3915 493 Size: 711 2150 67 494 2702 Fragments arranged by size: 2702 2150 711 494 67 BsaBI GATnn'nnATC Cuts at: 2969 6118 2969 Size: 3149 2975 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2848 3931 4313 4948 Size: 53 83 186 936 1315 1083 382 635 Cuts at: 4948 275 Size: 1451 Fragments arranged by size: 1451 1315 1083 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2214 4557 4955 5229 5376 5787 2214 Size: 2343 398 274 147 411 2551 Fragments arranged by size: 2551 2343 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2576 2606 3037 3067 1178 Size: 30 165 30 1173 30 431 30 4235 Fragments arranged by size: 4235 1173 431 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2216 2328 2597 1186 Size: 1030 112 269 4713 Fragments arranged by size: 4713 1030 269 112 BseYI C'CCAG_C Cuts at: 5327 5823 5327 Size: 496 5628 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5551 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3574 4335 5363 1540 Size: 275 186 1573 761 1028 2301 Fragments arranged by size: 2301 1573 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3133 4185 4270 5341 3133 Size: 1052 85 1071 3916 Fragments arranged by size: 3916 1071 1052 85 BsmI GAATG_Cn' Cuts at: 3635 3728 3635 Size: 93 6031 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3133 3443 4185 4270 5341 1391 Size: 399 1343 310 742 85 1071 2174 Fragments arranged by size: 2174 1343 1071 742 399 310 85 BspEI T'CCGG_A Cuts at: 2214 5787 2214 Size: 3573 2551 BspHI T'CATG_A Cuts at: 4013 5743 4013 Size: 1730 4394 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2401 3211 3479 844 Size: 1557 810 268 3489 Fragments arranged by size: 3489 1557 810 268 BsrBI CCG'CTC Cuts at: 1394 1823 4011 1394 Size: 429 2188 3507 Fragments arranged by size: 3507 2188 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2034 2376 2886 4619 4793 65 Size: 1183 786 342 510 1733 174 1396 Fragments arranged by size: 1733 1396 1183 786 510 342 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4768 4955 1700 Size: 110 2958 187 2869 Fragments arranged by size: 2958 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 4184 5196 4184 Size: 1012 5112 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6124 BstBI TT'CG_AA Cuts at: 2007 4928 2007 Size: 2921 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6124 BstYI r'GATC_y Cuts at: 1972 3085 3454 3821 4206 4223 5675 5761 5773 Size: 1113 369 367 385 17 1452 86 12 Cuts at: 5773 6119 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1113 385 369 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2895 2895 Size: 6124 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4615 Fragments arranged by size: 4615 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5385 Fragments arranged by size: 5385 683 56 BtsI GCAGTG_nn' Cuts at: 3640 4434 4454 3640 Size: 794 20 5310 Fragments arranged by size: 5310 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6089 DraI TTT'AAA Cuts at: 3428 3428 Size: 6124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2416 3291 3571 Size: 54 1425 50 275 18 586 875 280 Cuts at: 3571 4460 6089 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 875 586 280 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2255 2574 4054 1353 Size: 902 319 1480 3423 Fragments arranged by size: 3423 1480 902 319 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4711 5085 5231 387 Size: 1064 138 3122 374 146 1280 Fragments arranged by size: 3122 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3131 3131 Size: 6124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2553 3039 3156 4187 4784 5571 Size: 393 491 430 486 117 1031 597 787 Cuts at: 5571 5839 1239 Size: 268 1524 Fragments arranged by size: 1524 1031 787 597 491 486 430 393 268 117 EcoO109I rG'GnC_Cy Cuts at: 3370 3370 Size: 6124 EcoRI G'AATT_C Cuts at: 2809 3433 3811 2809 Size: 624 378 5122 Fragments arranged by size: 5122 624 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3177 3177 Size: 6124 FspI TGC'GCA Cuts at: 4630 4630 Size: 6124 HaeII r_GCGC'y Cuts at: 1678 3364 5271 1678 Size: 1686 1907 2531 Fragments arranged by size: 2531 1907 1686 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 2924 2956 3037 Size: 32 135 32 704 32 616 32 81 Cuts at: 3037 3069 3227 3259 4765 4797 4839 4871 1373 Size: 32 158 32 1506 32 42 32 2626 Fragments arranged by size: 2626 1506 704 616 158 135 81 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3178 3468 3714 669 Size: 988 281 1240 290 246 3079 Fragments arranged by size: 3079 1240 988 290 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6124 HpaI GTT'AAC Cuts at: 3714 3714 Size: 6124 KpnI G_GTAC'C Cuts at: 3449 3449 Size: 6124 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5238 5422 1305 Size: 3933 184 2007 Fragments arranged by size: 3933 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2418 3293 10 Size: 54 1425 343 586 875 2841 Fragments arranged by size: 2841 1425 875 586 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2364 2424 2444 4082 4441 4600 Size: 648 861 337 60 20 1638 359 159 Cuts at: 4600 518 Size: 2042 Fragments arranged by size: 2042 1638 861 648 359 337 159 60 20 MspA1I CmG'CkG Cuts at: 1606 3540 4217 5365 5610 1606 Size: 1934 677 1148 245 2120 Fragments arranged by size: 2120 1934 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4615 Fragments arranged by size: 4615 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6124 NheI G'CTAG_C Cuts at: 1052 2854 1052 Size: 1802 4322 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 2231 2363 3482 5027 1132 Size: 97 1002 132 1119 1545 2229 Fragments arranged by size: 2229 1545 1119 1002 132 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6124 PciI A'CATG_T Cuts at: 2359 5023 2359 Size: 2664 3460 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6124 PfoI T'CCnGG_A Cuts at: 3133 3133 Size: 6124 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4193 4225 5706 5738 4193 Size: 32 1481 32 4579 Fragments arranged by size: 4579 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2715 1613 Size: 1102 5022 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2322 2354 2322 Size: 32 6092 PstI C_TGCA'G Cuts at: 830 3476 830 Size: 2646 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 SacI G_AGCT'C Cuts at: 3133 3133 Size: 6124 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 4372 6084 4372 Size: 1712 4412 SfcI C'TryA_G Cuts at: 826 1045 2482 3472 4607 5288 5479 826 Size: 219 1437 990 1135 681 191 1471 Fragments arranged by size: 1471 1437 1135 990 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 3451 1872 Size: 1579 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3003 3278 4228 5129 5391 820 Size: 197 926 1060 275 950 901 262 1553 Fragments arranged by size: 1553 1060 950 926 901 275 262 197 SnaBI TAC'GTA Cuts at: 493 3421 3915 493 Size: 2928 494 2702 Fragments arranged by size: 2928 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 3482 1132 Size: 2350 3774 SspI AAT'ATT Cuts at: 5 52 4048 5 Size: 47 3996 2081 Fragments arranged by size: 3996 2081 47 StuI AGG'CCT Cuts at: 1517 2190 1517 Size: 673 5451 StyI C'CwwG_G Cuts at: 513 1065 2022 3081 3563 513 Size: 552 957 1059 482 3074 Fragments arranged by size: 3074 1059 957 552 482 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4151 4168 4321 4506 4951 325 Size: 1255 2571 17 153 185 445 1498 Fragments arranged by size: 2571 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4370 6082 96 Size: 274 80 33 51 1443 2393 1712 138 Fragments arranged by size: 2393 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6084 6116 6084 Size: 32 6092 Tth111I GACn'n_nGTC Cuts at: 1316 3335 1316 Size: 2019 4105 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2098 2098 Size: 6124 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 3449 1870 Size: 1579 4545 XmnI GAAnn'nnTTC Cuts at: 2477 4253 2477 Size: 1776 4348 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2849 3932 276 Size: 53 83 186 936 1315 1083 2468 Fragments arranged by size: 2468 1315 1083 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI