Restriction map for
pFN21ASDB1453
(Linear) (Six-base) MAPSORT of: KIBB1453.seq Check: 5959 from: 1 to: 1395
KIBB1453.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AatII G_ACGT'C
Cuts at: 0 828 1395
Size: 828 567
AfeI AGC'GCT
Cuts at: 0 1339 1395
Size: 1339 56
AflII C'TTAA_G
Cuts at: 0 1255 1395
Size: 1255 140
AflIII A'CryG_T
Cuts at: 0 336 1328 1395
Size: 336 992 67
Fragments arranged by size:
992 336 67
AleI CACnn'nnGTG
Cuts at: 0 401 1395
Size: 401 994
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 772 804 1395
Size: 772 32 591
Fragments arranged by size:
772 591 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1246 1395
Size: 1246 149
ApoI r'AATT_y
Cuts at: 0 84 730 786 1395
Size: 84 646 56 609
Fragments arranged by size:
646 609 84 56
BanII G_rGCy'C
Cuts at: 0 1110 1395
Size: 1110 285
BbsI GAAGACnn'nnnn_
Cuts at: 0 1308 1395
Size: 1308 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 84 118 1395
Size: 84 34 1277
Fragments arranged by size:
1277 84 34
BclI T'GATC_A
Cuts at: 0 929 1395
Size: 929 466
BmrI ACTGGGnnnn_n'
Cuts at: 0 755 1167 1320 1395
Size: 755 412 153 75
Fragments arranged by size:
755 412 153 75
BmtI G_CTAG'C
Cuts at: 0 835 1395
Size: 835 560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 530 1016 1133 1395
Size: 100 430 486 117 262
Fragments arranged by size:
486 430 262 117 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 965 1395
Size: 965 430
BsaI GGTCTCn'nnnn_
Cuts at: 0 967 1170 1395
Size: 967 203 225
Fragments arranged by size:
967 225 203
BsaAI yAC'GTr
Cuts at: 0 1331 1395
Size: 1331 64
BsaBI GATnn'nnATC
Cuts at: 0 946 1395
Size: 946 449
BsaHI Gr'CG_yC
Cuts at: 0 825 1395
Size: 825 570
BsaWI w'CCGG_w
Cuts at: 0 191 1395
Size: 191 1204
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 553 583 1014 1044 1395
Size: 553 30 431 30 351
Fragments arranged by size:
553 431 351 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 305 574 1395
Size: 193 112 269 821
Fragments arranged by size:
821 269 193 112
BsiHKAI G_wGCw'C
Cuts at: 0 1110 1395
Size: 1110 285
Bsp1286I G_dGCh'C
Cuts at: 0 1110 1395
Size: 1110 285
BspEI T'CCGG_A
Cuts at: 0 191 1395
Size: 191 1204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 378 1188 1395
Size: 378 810 207
Fragments arranged by size:
810 378 207
BsrDI GCAATG_nn'
Cuts at: 0 11 353 863 1395
Size: 11 342 510 532
Fragments arranged by size:
532 510 342 11
BstYI r'GATC_y
Cuts at: 0 1062 1395
Size: 1062 333
Bsu36I CC'TnA_GG
Cuts at: 0 872 1395
Size: 872 523
EaeI y'GGCC_r
Cuts at: 0 393 1268 1395
Size: 393 875 127
Fragments arranged by size:
875 393 127
EarI CTCTTCn'nnn_
Cuts at: 0 232 551 1395
Size: 232 319 844
Fragments arranged by size:
844 319 232
EcoICRI GAG'CTC
Cuts at: 0 1108 1395
Size: 1108 287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 530 1016 1133 1395
Size: 100 430 486 117 262
Fragments arranged by size:
486 430 262 117 100
EcoO109I rG'GnC_Cy
Cuts at: 0 1347 1395
Size: 1347 48
EcoRI G'AATT_C
Cuts at: 0 786 1395
Size: 786 609
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1154 1395
Size: 1154 241
HaeII r_GCGC'y
Cuts at: 0 1341 1395
Size: 1341 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 253 285 901 933 1014 1046 1204 1236
Size: 253 32 616 32 81 32 158 32
Cuts at: 1236 1395
Size: 159
Fragments arranged by size:
616 253 159 158 81 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1155 1395
Size: 1155 240
MscI TGG'CCA
Cuts at: 0 395 1270 1395
Size: 395 875 125
Fragments arranged by size:
875 395 125
MslI CAynn'nnrTG
Cuts at: 0 341 401 421 1395
Size: 341 60 20 974
Fragments arranged by size:
974 341 60 20
NheI G'CTAG_C
Cuts at: 0 831 1395
Size: 831 564
NspI r_CATG'y
Cuts at: 0 208 340 1395
Size: 208 132 1055
Fragments arranged by size:
1055 208 132
PciI A'CATG_T
Cuts at: 0 336 1395
Size: 336 1059
PfoI T'CCnGG_A
Cuts at: 0 1110 1395
Size: 1110 285
PshAI GACnn'nnGTC
Cuts at: 0 692 1395
Size: 692 703
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 299 331 1395
Size: 299 32 1064
Fragments arranged by size:
1064 299 32
SacI G_AGCT'C
Cuts at: 0 1110 1395
Size: 1110 285
SfcI C'TryA_G
Cuts at: 0 459 1395
Size: 459 936
SmlI C'TyrA_G
Cuts at: 0 980 1255 1395
Size: 980 275 140
Fragments arranged by size:
980 275 140
StuI AGG'CCT
Cuts at: 0 167 1395
Size: 167 1228
StyI C'CwwG_G
Cuts at: 0 1058 1395
Size: 1058 337
Tth111I GACn'n_nGTC
Cuts at: 0 1312 1395
Size: 1312 83
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 1395
Size: 75 1320
XmnI GAAnn'nnTTC
Cuts at: 0 454 1395
Size: 454 941
ZraI GAC'GTC
Cuts at: 0 826 1395
Size: 826 569
Enzymes that do cut:
AatII AfeI AflII AflIII AleI AloI AlwNI ApoI
BanII BbsI BcgI BclI BmrI BmtI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiHKAI
Bsp1286I BspEI BspMI BsrDI BstYI Bsu36I EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII
MscI MslI NheI NspI PciI PfoI PshAI PsrI
SacI SfcI SmlI StuI StyI Tth111I XcmI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AgeI AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BciVI BfrBI BglI BglII
BlpI Bme1580I BmgBI BplI Bpu10I BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI TstI
XbaI XhoI XmaI