Restriction map for
pFN21ASDB1316
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
ApaI AscI AseI AsnI AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BclI BglII BlnI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstYI BstZ17I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI MfeI MflI
MluI MluNI MroI MscI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XhoII
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1083 GGTACC G^GTACC
AccI 691 GTCTAC GT^[AC][GT]AC
AflII 2182 CTTAAG C^TTAAG
AflIII 371 ACGTGT A^C[GA][CT]GT
AocI 789 CCTGAGG CC^T[ACGT]AGG
Asp700I 569 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1083 GGTACC G^GTACC
AspI 685 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 2272 GGATAC GTATCC(6/5)
BfaI 626 CTAG C^TAG
BfrI 2182 CTTAAG C^TTAAG
BlpI 162 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 162 GCTGAGC GC^T[ACGT]AGC
BsaAI 370 TACGTG [CT]AC^GT[GA]
BsaBI 2133 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1277 ACCGGA [AT]^CCGG[AT]
BsmBI 2371 GAGACG CGTCTC(1/5)
BsmI 388 GCATTC GAATGC(1/-1)
Bsp1407I 185 TGTACA T^GTACA
BspHI 126 TCATGA T^CATGA
BsrGI 185 TGTACA T^GTACA
BstEII 1209 GGTAACC G^GT[ACGT]ACC
BstPI 1209 GGTAACC G^GT[ACGT]ACC
Bsu36I 789 CCTGAGG CC^T[ACGT]AGG
CelII 162 GCTGAGC GC^T[ACGT]AGC
EaeI 847 CGGCCA [CT]^GGCC[GA]
Eco81I 789 CCTGAGG CC^T[ACGT]AGG
EcoNI 1872 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1209 GGTAACC G^GT[ACGT]ACC
HaeII 355 AGCGCC [GA]GCGC^[CT]
KpnI 1083 GGTACC GGTAC^C
KspI 1093 CCGCGG CCGC^GG
MaeI 626 CTAG C^TAG
MamI 2133 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 396 GCCGGC GCC^GGC
NgoMIV 396 GCCGGC G^CCGGC
NspI 1025 ACATGC [GA]CATG^[CT]
PaeR7I 1360 CTCGAG C^TCGAG
PflFI 685 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 946 CAGCTG CAG^CTG
RcaI 126 TCATGA T^CATGA
SacI 1337 GAGCTC GAGCT^C
SacII 1093 CCGCGG CCGC^GG
SapI 1395 GCTCTTC GCTCTTC(1/4)
SexAI 113 ACCTGGT A^CC[AT]GGT
SfiI 1491 GGCCCCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 185 TGTACA T^GTACA
TfiI 1626 GATTC G^A[AT]TC
Tth111I 685 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1360 CTCGAG C^TCGAG
XmnI 569 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 528 GACGTC GACGT^C
2539 GACGTC
AccII 1094 CGCG CG^CG
1544 CGCG
AcyI 528 GACGTC G[GA]^CG[CT]C
2539 GACGTC
BcgI 832 CGACTCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2511 CGACCTTTCTGC
BpmI 1100 CTGGAG CTGGAG(16/14)
1563 CTGGAG
BsaHI 528 GACGTC G[GA]^CG[CT]C
2539 GACGTC
BsrBI 2301 CCGCTC CCGCTC(-3/-3)
1638 GAGCGG
BsrI 1414 CCAGT ACTGG(1/-1)
1866 CCAGT
BstUI 1094 CGCG CG^CG
1544 CGCG
BstXI 2009 CCAAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2400 CCAGCAGAATGG
DraIII 112 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1056 CACCAAGTG
EcoT14I 610 CCTTGG C^C[AT][AT]GG
2031 CCAAGG
HgaI 1167 GACGC GACGC(5/10)
2572 GACGC
Hin1I 528 GACGTC G[GA]^CG[CT]C
2539 GACGTC
MvnI 1094 CGCG CG^CG
1544 CGCG
PflMI 1263 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1284 CCAGGCCCTGG
PstI 343 CTGCAG CTGCA^G
1171 CTGCAG
SfcI 343 CTGCAG C^T[GA][CT]AG
1171 CTGCAG
SmlI 1360 CTCGAG C^T[CT][GA]AG
2182 CTTAAG
StyI 610 CCTTGG C^C[AT][AT]GG
2031 CCAAGG
Tsp45I 404 GTGAC ^GT[GC]AC
2348 GTGAC
Van91I 1263 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1284 CCAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BglI BmyI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmaI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I XcmI
XmaCI XmaI