(Circular) (Six-base) MAPSORT of: pFN21ASDB1316.seq Check: 7421 from: 1 to: 7315 pFN21ASDB1316 7315 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3554 3554 Size: 7315 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2555 4566 5125 278 Size: 53 83 186 936 1019 2011 559 2468 Fragments arranged by size: 2468 2011 1019 936 559 186 83 53 AccI GT'mk_AC Cuts at: 1937 2715 4658 1937 Size: 778 1943 4594 Fragments arranged by size: 4594 1943 778 Acc65I G'GTAC_C Cuts at: 3106 4636 3106 Size: 1530 5785 AclI AA'CG_TT Cuts at: 1563 5442 5815 1563 Size: 3879 373 3063 Fragments arranged by size: 3879 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2954 4409 4598 5378 6762 7030 1632 Size: 1322 1455 189 780 1384 268 1917 Fragments arranged by size: 1917 1455 1384 1322 780 268 189 AflII C'TTAA_G Cuts at: 820 1017 4205 820 Size: 197 3188 3930 Fragments arranged by size: 3930 3188 197 AflIII A'CryG_T Cuts at: 1203 2394 6214 7191 1203 Size: 1191 3820 977 1327 Fragments arranged by size: 3820 1327 1191 977 AgeI A'CCGG_T Cuts at: 6146 6146 Size: 7315 AhdI GACnn_n'nnGTC Cuts at: 6044 6044 Size: 7315 AleI CACnn'nnGTG Cuts at: 1166 2732 3062 4034 1166 Size: 1566 330 972 4447 Fragments arranged by size: 4447 1566 972 330 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2806 1184 Size: 1622 5693 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2429 2461 2429 Size: 32 7283 AlwNI CAG_nnn'CTG Cuts at: 3313 3448 3895 4387 5058 6630 3313 Size: 135 447 492 671 1572 3998 Fragments arranged by size: 3998 1572 671 492 447 135 ApaLI G'TGCA_C Cuts at: 2032 2132 2998 5372 6528 2032 Size: 100 866 2374 1156 2819 Fragments arranged by size: 2819 2374 1156 866 100 ApoI r'AATT_y Cuts at: 1493 2593 3175 4319 4624 4850 5002 7111 1493 Size: 1100 582 1144 305 226 152 2109 1697 Fragments arranged by size: 2109 1697 1144 1100 582 305 226 152 AseI AT'TA_AT Cuts at: 160 1032 5869 160 Size: 872 4837 1606 Fragments arranged by size: 4837 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7315 AvaI C'yCGr_G Cuts at: 1870 1943 2220 2926 3383 3499 3771 4640 1870 Size: 73 277 706 457 116 272 869 4545 Fragments arranged by size: 4545 869 706 457 277 272 116 73 BamHI G'GATC_C Cuts at: 4645 5012 4645 Size: 367 6948 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2216 2938 3106 3542 4636 Size: 325 217 627 429 722 168 436 1094 Cuts at: 4636 6091 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1094 722 627 436 429 325 217 168 BanII G_rGCy'C Cuts at: 1391 3364 3593 3635 3818 4634 1391 Size: 1973 229 42 183 816 4072 Fragments arranged by size: 4072 1973 816 229 183 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2224 2301 2881 2902 3022 3450 3919 Size: 971 325 77 580 21 120 428 469 Cuts at: 3919 4053 928 Size: 134 4190 Fragments arranged by size: 4190 971 580 469 428 325 134 120 77 21 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2844 2878 4523 4557 5506 5540 1903 Size: 34 907 34 1645 34 949 34 3678 Fragments arranged by size: 3678 1645 949 907 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4289 5207 6417 4289 Size: 918 1210 5187 Fragments arranged by size: 5187 1210 918 BclI T'GATC_A Cuts at: 1548 1548 Size: 7315 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2225 2697 3521 Size: 107 122 71 631 605 553 472 824 Cuts at: 3521 4085 5926 136 Size: 564 1841 1525 Fragments arranged by size: 1841 1525 824 631 605 564 553 472 122 107 71 BglII A'GATC_T Cuts at: 7310 7310 Size: 7315 BlpI GC'TnA_GC Cuts at: 2186 4732 2186 Size: 2546 4769 Bme1580I G_kGCm'C Cuts at: 1790 2036 2136 2221 3002 3683 5376 6532 1790 Size: 246 100 85 781 681 1693 1156 2573 Fragments arranged by size: 2573 1693 1156 781 681 246 100 85 BmgBI CAC'GTC Cuts at: 1318 2668 3949 1318 Size: 1350 1281 4684 Fragments arranged by size: 4684 1350 1281 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5046 6004 442 Size: 416 53 83 399 24 3629 958 1753 Fragments arranged by size: 3629 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7315 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3049 3049 Size: 7315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3144 3607 5975 1239 Size: 1905 463 2368 2579 Fragments arranged by size: 2579 2368 1905 463 Bpu10I CC'TnA_GC Cuts at: 2918 2918 Size: 7315 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5440 6305 6603 5440 Size: 865 298 6152 Fragments arranged by size: 6152 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2293 3404 4463 5978 882 Size: 617 794 1111 1059 1515 2219 Fragments arranged by size: 2219 1515 1111 1059 794 617 BsaAI yAC'GTr Cuts at: 493 1204 2395 4612 5106 493 Size: 711 1191 2217 494 2702 Fragments arranged by size: 2702 2217 1191 711 494 BsaBI GATnn'nnATC Cuts at: 4160 7309 4160 Size: 3149 4166 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2552 4563 5122 5504 Size: 53 83 186 936 1019 2011 559 382 Cuts at: 5504 6139 275 Size: 635 1451 Fragments arranged by size: 2011 1451 1019 936 635 559 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3300 5748 6146 6420 6567 6978 3300 Size: 2448 398 274 147 411 3637 Fragments arranged by size: 3637 2448 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2735 2765 3152 3182 4099 Size: 30 165 30 1332 30 387 30 917 Cuts at: 4099 4129 4596 4626 1178 Size: 30 467 30 3867 Fragments arranged by size: 3867 1332 917 467 387 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3038 3422 3917 4084 4217 1186 Size: 1852 384 495 167 133 4284 Fragments arranged by size: 4284 1852 495 384 167 133 BseYI C'CCAG_C Cuts at: 2500 3414 3635 4075 4176 4422 6518 7014 2500 Size: 914 221 440 101 246 2096 496 2801 Fragments arranged by size: 2801 2096 914 496 440 246 221 101 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 2334 3350 3602 4103 1163 Size: 573 426 172 1016 252 501 4375 Fragments arranged by size: 4375 1016 573 501 426 252 172 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4765 5526 6554 1540 Size: 275 186 2764 761 1028 2301 Fragments arranged by size: 2764 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2036 2124 2136 2919 3002 3059 3364 3390 4440 Size: 88 12 783 83 57 305 26 1050 Cuts at: 4440 5376 5461 6532 2036 Size: 936 85 1071 2819 Fragments arranged by size: 2819 1071 1050 936 783 305 88 85 83 57 26 12 BsmI GAATG_Cn' Cuts at: 2411 4826 4919 2411 Size: 2415 93 4807 Fragments arranged by size: 4807 2415 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4388 4388 Size: 7315 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2124 2136 2221 2919 3002 3059 Size: 399 246 88 12 85 698 83 57 Cuts at: 3059 3364 3390 3593 3635 3683 3818 4440 4634 Size: 305 26 203 42 48 135 622 194 Cuts at: 4634 5376 5461 6532 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 698 622 399 305 246 203 194 135 88 85 85 83 57 48 42 26 12 BspEI T'CCGG_A Cuts at: 6978 6978 Size: 7315 BspHI T'CATG_A Cuts at: 2149 5204 6934 2149 Size: 3055 1730 2530 Fragments arranged by size: 3055 2530 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3554 3614 4308 4670 844 Size: 2710 60 694 362 3489 Fragments arranged by size: 3489 2710 694 362 60 BsrBI CCG'CTC Cuts at: 1394 1823 3663 4326 5202 1394 Size: 429 1840 663 876 3507 Fragments arranged by size: 3507 1840 876 663 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5810 5984 65 Size: 1183 4562 174 1396 Fragments arranged by size: 4562 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2419 3705 4133 4460 5959 6146 1700 Size: 110 609 1286 428 327 1499 187 2869 Fragments arranged by size: 2869 1499 1286 609 428 327 187 110 BsrGI T'GTAC_A Cuts at: 96 2208 96 Size: 2112 5203 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7315 BssSI C'ACGA_G Cuts at: 5375 6387 5375 Size: 1012 6303 BstAPI GCAn_nnn'nTGC Cuts at: 794 2140 2312 4143 794 Size: 1346 172 1831 3966 Fragments arranged by size: 3966 1831 1346 172 BstBI TT'CG_AA Cuts at: 2007 6119 2007 Size: 4112 3203 BstEII G'GTnAC_C Cuts at: 1184 3232 1184 Size: 2048 5267 BstXI CCAn_nnnn'nTGG Cuts at: 4039 4430 4039 Size: 391 6924 BstYI r'GATC_y Cuts at: 1972 4645 5012 5397 5414 6866 6952 6964 7310 Size: 2673 367 385 17 1452 86 12 346 Cuts at: 7310 1972 Size: 1977 Fragments arranged by size: 2673 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2813 2813 Size: 7315 BtgI C'CryG_G Cuts at: 513 1065 2774 3037 3116 4503 513 Size: 552 1709 263 79 1387 3325 Fragments arranged by size: 3325 1709 1387 552 263 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2099 3681 487 Size: 683 56 873 1582 4121 Fragments arranged by size: 4121 1582 873 683 56 BtsI GCAGTG_nn' Cuts at: 2073 3000 4831 5625 5645 2073 Size: 927 1831 794 20 3743 Fragments arranged by size: 3743 1831 927 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7315 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3709 3744 529 Size: 35 3145 35 4100 Fragments arranged by size: 4100 3145 35 35 DraI TTT'AAA Cuts at: 4619 4619 Size: 7315 DraIII CAC_nnn'GTG Cuts at: 2140 3084 2140 Size: 944 6371 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6322 809 Size: 507 5006 1802 Fragments arranged by size: 5006 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2870 4762 5651 Size: 54 1425 50 275 18 1040 1892 889 Cuts at: 5651 7280 8 Size: 1629 43 Fragments arranged by size: 1892 1629 1425 1040 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4762 1537 Size: 275 2950 4090 Fragments arranged by size: 4090 2950 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2963 3425 4244 4250 4265 5245 1353 Size: 1610 462 819 6 15 980 3423 Fragments arranged by size: 3423 1610 980 819 462 15 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5902 6276 6422 387 Size: 1064 138 4313 374 146 1280 Fragments arranged by size: 4313 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3362 3362 Size: 7315 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2954 3144 3607 4409 4598 5378 5975 Size: 393 1322 190 463 802 189 780 597 Cuts at: 5975 6762 7030 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1322 802 787 780 597 463 393 268 190 189 EcoNI CCTnn'n_nnAGG Cuts at: 3899 3899 Size: 7315 EcoO109I rG'GnC_Cy Cuts at: 2817 2922 3310 3514 3571 2817 Size: 105 388 204 57 6561 Fragments arranged by size: 6561 388 204 105 57 EcoRI G'AATT_C Cuts at: 4624 5002 4624 Size: 378 6937 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2221 2221 Size: 7315 FspI TGC'GCA Cuts at: 5821 5821 Size: 7315 HaeII r_GCGC'y Cuts at: 1678 2382 6462 1678 Size: 704 4080 2531 Fragments arranged by size: 4080 2531 704 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3117 3149 5956 5988 6030 Size: 32 135 32 1545 32 2807 32 42 Cuts at: 6030 6062 1373 Size: 32 2626 Fragments arranged by size: 2807 2626 1545 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4659 4905 669 Size: 988 281 2721 246 3079 Fragments arranged by size: 3079 2721 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7315 HpaI GTT'AAC Cuts at: 4905 4905 Size: 7315 KpnI G_GTAC'C Cuts at: 3110 4640 3110 Size: 1530 5785 MfeI C'AATT_G Cuts at: 4914 4914 Size: 7315 MluI A'CGCG_T Cuts at: 7191 7191 Size: 7315 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3786 6429 6613 1305 Size: 2481 2643 184 2007 Fragments arranged by size: 2643 2481 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5493 Fragments arranged by size: 5493 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2360 2732 3062 4034 5273 5632 Size: 648 861 333 372 330 972 1239 359 Cuts at: 5632 5791 518 Size: 159 2042 Fragments arranged by size: 2042 1239 972 861 648 372 359 333 330 159 MspA1I CmG'CkG Cuts at: 1606 2880 2971 3118 3520 3737 4731 5408 6556 Size: 1274 91 147 402 217 994 677 1148 Cuts at: 6556 6801 1606 Size: 245 2120 Fragments arranged by size: 2120 1274 1148 994 677 402 245 217 147 91 NaeI GCC'GGC Cuts at: 2421 2421 Size: 7315 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6763 NdeI CA'TA_TG Cuts at: 387 387 Size: 7315 NgoMIV G'CCGG_C Cuts at: 2419 2419 Size: 7315 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7315 NotI GC'GGCC_GC Cuts at: 4762 4762 Size: 7315 NspI r_CATG'y Cuts at: 1132 1229 3052 4673 6218 1132 Size: 97 1823 1621 1545 2229 Fragments arranged by size: 2229 1823 1621 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7315 PciI A'CATG_T Cuts at: 6214 6214 Size: 7315 PflMI CCAn_nnn'nTGG Cuts at: 1108 3292 3313 1108 Size: 2184 21 5110 Fragments arranged by size: 5110 2184 21 PfoI T'CCnGG_A Cuts at: 3423 3951 3423 Size: 528 6787 PmeI GTTT'AAAC Cuts at: 4619 4619 Size: 7315 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2741 2773 4565 4597 5384 5416 6897 6929 2741 Size: 32 1792 32 787 32 1481 32 3127 Fragments arranged by size: 3127 1792 1481 787 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2817 2922 3571 2817 Size: 105 649 6561 Fragments arranged by size: 6561 649 105 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7315 PsiI TTA'TAA Cuts at: 4885 4885 Size: 7315 PspXI vC'TCGA_Gb Cuts at: 3383 3383 Size: 7315 PstI C_TGCA'G Cuts at: 830 2370 3198 4667 830 Size: 1540 828 1469 3478 Fragments arranged by size: 3478 1540 1469 828 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7315 PvuII CAG'CTG Cuts at: 2971 2971 Size: 7315 SacI G_AGCT'C Cuts at: 3364 3364 Size: 7315 SacII CC_GC'GG Cuts at: 3119 3119 Size: 7315 SalI G'TCGA_C Cuts at: 1936 4657 1936 Size: 2721 4594 SanDI GG'GwC_CC Cuts at: 3571 3571 Size: 7315 SapI GCTCTTCn'nnn_ Cuts at: 3425 3425 Size: 7315 SbfI CC_TGCA'GG Cuts at: 4667 4667 Size: 7315 ScaI AGT'ACT Cuts at: 5563 7275 5563 Size: 1712 5603 SexAI A'CCwGG_T Cuts at: 2136 2136 Size: 7315 SfcI C'TryA_G Cuts at: 826 1045 2366 3194 4663 5798 6479 6670 826 Size: 219 1321 828 1469 1135 681 191 1471 Fragments arranged by size: 1471 1469 1321 1135 828 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3521 3521 Size: 7315 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7315 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7315 SmaI CCC'GGG Cuts at: 1872 2222 2928 3773 4642 1872 Size: 350 706 845 869 4545 Fragments arranged by size: 4545 869 845 706 350 SmlI C'TyrA_G Cuts at: 820 1017 1943 3383 4205 5419 6320 6582 820 Size: 197 926 1440 822 1214 901 262 1553 Fragments arranged by size: 1553 1440 1214 926 901 822 262 197 SnaBI TAC'GTA Cuts at: 493 4612 5106 493 Size: 4119 494 2702 Fragments arranged by size: 4119 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7315 SphI G_CATG'C Cuts at: 1132 4673 1132 Size: 3541 3774 SspI AAT'ATT Cuts at: 5 52 5239 5 Size: 47 5187 2081 Fragments arranged by size: 5187 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7315 StyI C'CwwG_G Cuts at: 513 1065 2633 4054 4754 513 Size: 552 1568 1421 700 3074 Fragments arranged by size: 3074 1568 1421 700 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2113 3076 3299 3641 4067 4487 5342 Size: 1255 533 963 223 342 426 420 855 Cuts at: 5342 5359 5512 5697 6142 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 963 855 533 445 426 420 342 223 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2208 3045 5561 Size: 274 80 33 51 1443 231 837 2516 Cuts at: 5561 7273 96 Size: 1712 138 Fragments arranged by size: 2516 1712 1443 837 274 231 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4099 4131 7275 7307 4099 Size: 32 3144 32 4107 Fragments arranged by size: 4107 3144 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2711 1316 Size: 1395 5920 XbaI T'CTAG_A Cuts at: 4651 4651 Size: 7315 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3314 3949 4036 4039 4450 3314 Size: 635 87 3 411 6179 Fragments arranged by size: 6179 635 411 87 3 XhoI C'TCGA_G Cuts at: 1943 3383 1943 Size: 1440 5875 XmaI C'CCGG_G Cuts at: 1870 2220 2926 3771 4640 1870 Size: 350 706 845 869 4545 Fragments arranged by size: 4545 869 845 706 350 XmnI GAAnn'nnTTC Cuts at: 2596 5444 2596 Size: 2848 4467 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2553 4564 5123 276 Size: 53 83 186 936 1019 2011 559 2468 Fragments arranged by size: 2468 2011 1019 936 559 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SfoI SrfI SwaI