(Circular) (Six-base) MAPSORT of: pFN21ASDB0234.seq Check: 4988 from: 1 to: 8338 pFN21ASDB0234 8338 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6148 278 Size: 53 83 186 936 4612 2468 Fragments arranged by size: 4612 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2211 4166 5681 1937 Size: 274 1955 1515 4594 Fragments arranged by size: 4594 1955 1515 274 Acc65I G'GTAC_C Cuts at: 2825 4299 5659 2825 Size: 1474 1360 5504 Fragments arranged by size: 5504 1474 1360 AclI AA'CG_TT Cuts at: 1563 6465 6838 1563 Size: 4902 373 3063 Fragments arranged by size: 4902 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2374 4978 5250 5310 5597 6401 7785 8053 Size: 742 2604 272 60 287 804 1384 268 Cuts at: 8053 1632 Size: 1917 Fragments arranged by size: 2604 1917 1384 804 742 287 272 268 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8141 AflIII A'CryG_T Cuts at: 1203 7237 8214 1203 Size: 6034 977 1327 Fragments arranged by size: 6034 1327 977 AgeI A'CCGG_T Cuts at: 7169 7169 Size: 8338 AhdI GACnn_n'nnGTC Cuts at: 7067 7067 Size: 8338 AleI CACnn'nnGTG Cuts at: 1166 3203 4322 4995 1166 Size: 2037 1119 673 4509 Fragments arranged by size: 4509 2037 1119 673 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8338 AlwNI CAG_nnn'CTG Cuts at: 2528 3458 3650 3824 5466 6081 7653 2528 Size: 930 192 174 1642 615 1572 3213 Fragments arranged by size: 3213 1642 1572 930 615 192 174 ApaI G_GGCC'C Cuts at: 4787 5042 4787 Size: 255 8083 ApaLI G'TGCA_C Cuts at: 5305 6395 7551 5305 Size: 1090 1156 6092 Fragments arranged by size: 6092 1156 1090 ApoI r'AATT_y Cuts at: 1493 2274 3323 5195 5647 5873 6025 8134 1493 Size: 781 1049 1872 452 226 152 2109 1697 Fragments arranged by size: 2109 1872 1697 1049 781 452 226 152 AseI AT'TA_AT Cuts at: 160 1032 6892 160 Size: 872 5860 1606 Fragments arranged by size: 5860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8338 AvaI C'yCGr_G Cuts at: 1870 1943 3042 5277 5663 1870 Size: 73 1099 2235 386 4545 Fragments arranged by size: 4545 2235 1099 386 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2661 2694 2817 2850 2661 Size: 33 123 33 8149 Fragments arranged by size: 8149 123 33 33 BamHI G'GATC_C Cuts at: 3883 5668 6035 3883 Size: 1785 367 6186 Fragments arranged by size: 6186 1785 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2321 2825 2897 3128 3314 Size: 325 217 627 534 504 72 231 186 Cuts at: 3314 4081 4299 4487 4580 4789 5659 7114 618 Size: 767 218 188 93 209 870 1455 1842 Fragments arranged by size: 1842 1455 870 767 627 534 504 325 231 218 217 209 188 186 93 72 BanII G_rGCy'C Cuts at: 1391 4787 5042 5657 1391 Size: 3396 255 615 4072 Fragments arranged by size: 4072 3396 615 255 BbeI G_GCGC'C Cuts at: 2325 2325 Size: 8338 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3516 5481 928 Size: 971 1617 1965 3785 Fragments arranged by size: 3785 1965 1617 971 BbvCI CC'TCA_GC Cuts at: 2334 2334 Size: 8338 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6529 6563 1903 Size: 34 4592 34 3678 Fragments arranged by size: 4592 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2266 2416 6230 7440 2266 Size: 150 3814 1210 3164 Fragments arranged by size: 3814 3164 1210 150 BclI T'GATC_A Cuts at: 1548 2397 2490 1548 Size: 849 93 7396 Fragments arranged by size: 7396 849 93 BfrBI ATG'CAT Cuts at: 4544 4544 Size: 8338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6949 136 Size: 107 122 71 631 605 5277 1525 Fragments arranged by size: 5277 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8333 8333 Size: 8338 BlpI GC'TnA_GC Cuts at: 5755 5755 Size: 8338 Bme1580I G_kGCm'C Cuts at: 1790 2139 2902 3317 4086 4787 5042 5309 5451 Size: 349 763 415 769 701 255 267 142 Cuts at: 5451 6399 7555 1790 Size: 948 1156 2573 Fragments arranged by size: 2573 1156 948 769 763 701 415 349 267 255 142 BmgBI CAC'GTC Cuts at: 1318 3473 1318 Size: 2155 6183 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2277 2958 3357 Size: 416 53 83 399 24 860 681 399 Cuts at: 3357 4034 4789 6069 7027 442 Size: 677 755 1280 958 1753 Fragments arranged by size: 1753 1280 958 860 755 681 677 416 399 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8338 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2327 2327 Size: 8338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2592 3586 3846 3915 4772 4869 6998 1239 Size: 1353 994 260 69 857 97 2129 2579 Fragments arranged by size: 2579 2129 1353 994 857 260 97 69 Bpu10I CC'TnA_GC Cuts at: 2334 2334 Size: 8338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2076 2381 3419 5295 6463 7328 7626 2076 Size: 305 1038 1876 1168 865 298 2788 Fragments arranged by size: 2788 1876 1168 1038 865 305 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3281 4394 5121 7001 882 Size: 617 1782 1113 727 1880 2219 Fragments arranged by size: 2219 1880 1782 1113 727 617 BsaAI yAC'GTr Cuts at: 493 1204 4909 5635 6129 493 Size: 711 3705 726 494 2702 Fragments arranged by size: 3705 2702 726 711 494 BsaBI GATnn'nnATC Cuts at: 2684 4475 8332 2684 Size: 1791 3857 2690 Fragments arranged by size: 3857 2690 1791 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2322 6145 6527 7162 Size: 53 83 186 936 789 3823 382 635 Cuts at: 7162 275 Size: 1451 Fragments arranged by size: 3823 1451 936 789 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2883 4200 5215 6771 7169 7443 7590 8001 2883 Size: 1317 1015 1556 398 274 147 411 3220 Fragments arranged by size: 3220 1556 1317 1015 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2330 2360 3014 3044 4391 Size: 30 165 30 927 30 654 30 1347 Cuts at: 4391 4421 1178 Size: 30 5095 Fragments arranged by size: 5095 1347 927 654 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 2999 4582 4769 5379 1186 Size: 1165 648 1583 187 610 4145 Fragments arranged by size: 4145 1583 1165 648 610 187 BseYI C'CCAG_C Cuts at: 2231 7541 8037 2231 Size: 5310 496 2532 Fragments arranged by size: 5310 2532 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5029 1163 Size: 573 3293 4472 Fragments arranged by size: 4472 3293 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5788 6549 7577 1540 Size: 275 186 3787 761 1028 2301 Fragments arranged by size: 3787 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5309 5625 6399 6484 7555 5309 Size: 316 774 85 1071 6092 Fragments arranged by size: 6092 1071 774 316 85 BsmI GAATG_Cn' Cuts at: 2818 3559 5849 5942 2818 Size: 741 2290 93 5214 Fragments arranged by size: 5214 2290 741 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2902 3317 4086 4787 5042 5309 Size: 399 349 763 415 769 701 255 267 Cuts at: 5309 5451 5625 5657 6399 6484 7555 1391 Size: 142 174 32 742 85 1071 2174 Fragments arranged by size: 2174 1071 769 763 742 701 415 399 349 267 255 174 142 85 32 BspEI T'CCGG_A Cuts at: 4200 5215 8001 4200 Size: 1015 2786 4537 Fragments arranged by size: 4537 2786 1015 BspHI T'CATG_A Cuts at: 4467 4711 6227 7957 4467 Size: 244 1516 1730 4848 Fragments arranged by size: 4848 1730 1516 244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2728 5693 844 Size: 1884 2965 3489 Fragments arranged by size: 3489 2965 1884 BsrBI CCG'CTC Cuts at: 1394 1823 6225 1394 Size: 429 4402 3507 Fragments arranged by size: 4402 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6833 7007 65 Size: 1183 5585 174 1396 Fragments arranged by size: 5585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4484 4741 5137 6982 7169 1700 Size: 110 2674 257 396 1845 187 2869 Fragments arranged by size: 2869 2674 1845 396 257 187 110 BsrGI T'GTAC_A Cuts at: 96 2548 96 Size: 2452 5886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8338 BssSI C'ACGA_G Cuts at: 6398 7410 6398 Size: 1012 7326 BstAPI GCAn_nnn'nTGC Cuts at: 794 5600 794 Size: 4806 3532 BstBI TT'CG_AA Cuts at: 2007 7142 2007 Size: 5135 3203 BstEII G'GTnAC_C Cuts at: 1184 3052 1184 Size: 1868 6470 BstYI r'GATC_y Cuts at: 1972 2158 2524 3151 3883 5668 6035 6420 6437 Size: 186 366 627 732 1785 367 385 17 Cuts at: 6437 7889 7975 7987 8333 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1785 1452 732 627 385 367 366 346 186 86 17 12 BstZ17I GTA'TAC Cuts at: 4167 4167 Size: 8338 Bsu36I CC'TnA_GG Cuts at: 2030 3101 5616 2030 Size: 1071 2515 4752 Fragments arranged by size: 4752 2515 1071 BtgI C'CryG_G Cuts at: 513 1065 2022 4107 4491 513 Size: 552 957 2085 384 4360 Fragments arranged by size: 4360 2085 957 552 384 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4465 487 Size: 683 56 3239 4360 Fragments arranged by size: 4360 3239 683 56 BtsI GCAGTG_nn' Cuts at: 4134 4350 5116 5854 6648 6668 4134 Size: 216 766 738 794 20 5804 Fragments arranged by size: 5804 794 766 738 216 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3454 3489 529 Size: 35 2890 35 5378 Fragments arranged by size: 5378 2890 35 35 DraI TTT'AAA Cuts at: 2779 2937 3334 3369 5642 2779 Size: 158 397 35 2273 5475 Fragments arranged by size: 5475 2273 397 158 35 DraIII CAC_nnn'GTG Cuts at: 3004 4909 3004 Size: 1905 6433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7345 809 Size: 507 6029 1802 Fragments arranged by size: 6029 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2624 2727 4827 Size: 54 1425 50 275 18 794 103 2100 Cuts at: 4827 5785 6674 8303 8 Size: 958 889 1629 43 Fragments arranged by size: 2100 1629 1425 958 889 794 275 103 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5785 1537 Size: 275 3973 4090 Fragments arranged by size: 4090 3973 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3298 4680 4978 5175 6268 1353 Size: 1945 1382 298 197 1093 3423 Fragments arranged by size: 3423 1945 1382 1093 298 197 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6925 7299 7445 387 Size: 1064 138 5336 374 146 1280 Fragments arranged by size: 5336 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2374 2592 3586 3846 3915 4772 4869 Size: 393 742 218 994 260 69 857 97 Cuts at: 4869 4978 5250 5310 5597 6401 6998 7785 8053 Size: 109 272 60 287 804 597 787 268 Cuts at: 8053 1239 Size: 1524 Fragments arranged by size: 1524 994 857 804 787 742 597 393 287 272 268 260 218 109 97 69 60 EcoNI CCTnn'n_nnAGG Cuts at: 2068 2532 2068 Size: 464 7874 EcoO109I rG'GnC_Cy Cuts at: 2970 3241 3666 4783 5038 5039 5273 2970 Size: 271 425 1117 255 1 234 6035 Fragments arranged by size: 6035 1117 425 271 255 234 1 EcoRI G'AATT_C Cuts at: 3323 5195 5647 6025 3323 Size: 1872 452 378 5636 Fragments arranged by size: 5636 1872 452 378 EcoRV GAT'ATC Cuts at: 2596 2596 Size: 8338 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5059 5059 Size: 8338 FspI TGC'GCA Cuts at: 6844 6844 Size: 8338 HaeII r_GCGC'y Cuts at: 1678 2202 2325 5068 7485 1678 Size: 524 123 2743 2417 2531 Fragments arranged by size: 2743 2531 2417 524 123 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2580 2612 3014 3046 5195 Size: 32 135 32 1008 32 402 32 2149 Cuts at: 5195 5227 5228 5240 5260 5272 5293 5325 6979 Size: 32 1 12 20 12 21 32 1654 Cuts at: 6979 7011 7053 7085 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2149 1654 1008 402 135 42 32 32 32 32 32 32 32 32 21 20 12 12 1 HincII GTy'rAC Cuts at: 669 1657 1938 4138 4282 4345 5682 5928 669 Size: 988 281 2200 144 63 1337 246 3079 Fragments arranged by size: 3079 2200 1337 988 281 246 144 63 HindIII A'AGCT_T Cuts at: 748 3395 5435 748 Size: 2647 2040 3651 Fragments arranged by size: 3651 2647 2040 HpaI GTT'AAC Cuts at: 4345 5928 4345 Size: 1583 6755 KasI G'GCGC_C Cuts at: 2321 2321 Size: 8338 KpnI G_GTAC'C Cuts at: 2829 4303 5663 2829 Size: 1474 1360 5504 Fragments arranged by size: 5504 1474 1360 MfeI C'AATT_G Cuts at: 5937 5937 Size: 8338 MluI A'CGCG_T Cuts at: 8214 8214 Size: 8338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 3282 3861 4389 4820 7452 7636 1305 Size: 1008 969 579 528 431 2632 184 2007 Fragments arranged by size: 2632 2007 1008 969 579 528 431 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2626 4829 10 Size: 54 1425 343 794 2203 3519 Fragments arranged by size: 3519 2203 1425 794 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2178 3203 4145 4322 4505 4995 6296 Size: 648 1012 1025 942 177 183 490 1301 Cuts at: 6296 6655 6814 518 Size: 359 159 2042 Fragments arranged by size: 2042 1301 1025 1012 942 648 490 359 183 177 159 MspA1I CmG'CkG Cuts at: 1606 5754 6431 7579 7824 1606 Size: 4148 677 1148 245 2120 Fragments arranged by size: 4148 2120 1148 677 245 NaeI GCC'GGC Cuts at: 4743 4743 Size: 8338 NarI GG'CG_CC Cuts at: 2322 2322 Size: 8338 NcoI C'CATG_G Cuts at: 513 1065 2022 4491 513 Size: 552 957 2469 4360 Fragments arranged by size: 4360 2469 957 552 NdeI CA'TA_TG Cuts at: 387 2329 387 Size: 1942 6396 NgoMIV G'CCGG_C Cuts at: 4741 4741 Size: 8338 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8338 NotI GC'GGCC_GC Cuts at: 5785 5785 Size: 8338 NsiI A_TGCA'T Cuts at: 4546 4546 Size: 8338 NspI r_CATG'y Cuts at: 1132 1229 5696 7241 1132 Size: 97 4467 1545 2229 Fragments arranged by size: 4467 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8338 PciI A'CATG_T Cuts at: 7237 7237 Size: 8338 PflMI CCAn_nnn'nTGG Cuts at: 1108 5466 1108 Size: 4358 3980 PmeI GTTT'AAAC Cuts at: 5642 5642 Size: 8338 PmlI CAC'GTG Cuts at: 4909 4909 Size: 8338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6407 6439 7920 7952 6407 Size: 32 1481 32 6793 Fragments arranged by size: 6793 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2970 3241 3666 5273 2970 Size: 271 425 1607 6035 Fragments arranged by size: 6035 1607 425 271 PshAI GACnn'nnGTC Cuts at: 1613 5118 1613 Size: 3505 4833 PsiI TTA'TAA Cuts at: 3113 5586 5908 3113 Size: 2473 322 5543 Fragments arranged by size: 5543 2473 322 PspOMI G'GGCC_C Cuts at: 4783 5038 4783 Size: 255 8083 PspXI vC'TCGA_Gb Cuts at: 3042 3042 Size: 8338 PstI C_TGCA'G Cuts at: 830 2739 5111 5690 830 Size: 1909 2372 579 3478 Fragments arranged by size: 3478 2372 1909 579 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8338 SalI G'TCGA_C Cuts at: 1936 5680 1936 Size: 3744 4594 SanDI GG'GwC_CC Cuts at: 3666 5273 3666 Size: 1607 6731 SapI GCTCTTCn'nnn_ Cuts at: 4978 4978 Size: 8338 SbfI CC_TGCA'GG Cuts at: 5690 5690 Size: 8338 ScaI AGT'ACT Cuts at: 3790 6586 8298 3790 Size: 2796 1712 3830 Fragments arranged by size: 3830 2796 1712 SfcI C'TryA_G Cuts at: 826 1045 2212 2735 4170 5107 5295 5686 6821 Size: 219 1167 523 1435 937 188 391 1135 Cuts at: 6821 7502 7693 826 Size: 681 191 1471 Fragments arranged by size: 1471 1435 1167 1135 937 681 523 391 219 191 188 SfoI GGC'GCC Cuts at: 2323 2323 Size: 8338 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8338 SgrAI Cr'CCGG_yG Cuts at: 1700 4484 1700 Size: 2784 5554 SmaI CCC'GGG Cuts at: 1872 5665 1872 Size: 3793 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2091 2360 3042 3398 5310 6442 Size: 197 926 148 269 682 356 1912 1132 Cuts at: 6442 7343 7605 820 Size: 901 262 1553 Fragments arranged by size: 1912 1553 1132 926 901 682 356 269 262 197 148 SnaBI TAC'GTA Cuts at: 493 5635 6129 493 Size: 5142 494 2702 Fragments arranged by size: 5142 2702 494 SpeI A'CTAG_T Cuts at: 152 4979 152 Size: 4827 3511 SphI G_CATG'C Cuts at: 1132 5696 1132 Size: 4564 3774 SspI AAT'ATT Cuts at: 5 52 2467 3278 6262 5 Size: 47 2415 811 2984 2081 Fragments arranged by size: 2984 2415 2081 811 47 StuI AGG'CCT Cuts at: 1517 5210 1517 Size: 3693 4645 StyI C'CwwG_G Cuts at: 513 1065 2022 4491 5017 5777 513 Size: 552 957 2469 526 760 3074 Fragments arranged by size: 3074 2469 957 760 552 526 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3565 3591 5410 6365 6382 6535 6720 Size: 1255 1985 26 1819 955 17 153 185 Cuts at: 6720 7165 325 Size: 445 1498 Fragments arranged by size: 1985 1819 1498 1255 955 445 185 153 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2548 3788 6584 Size: 274 80 33 51 1443 571 1240 2796 Cuts at: 6584 8296 96 Size: 1712 138 Fragments arranged by size: 2796 1712 1443 1240 571 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2330 2362 8298 8330 2330 Size: 32 5936 32 2338 Fragments arranged by size: 5936 2338 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8338 XbaI T'CTAG_A Cuts at: 5674 5674 Size: 8338 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5537 5537 Size: 8338 XhoI C'TCGA_G Cuts at: 1943 3042 1943 Size: 1099 7239 XmaI C'CCGG_G Cuts at: 1870 5663 1870 Size: 3793 4545 XmnI GAAnn'nnTTC Cuts at: 5492 6467 5492 Size: 975 7363 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6146 276 Size: 53 83 186 936 4612 2468 Fragments arranged by size: 4612 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BsiWI BsmBI BstXI EcoICRI FseI FspAI NruI PacI PfoI PsrI PvuII RsrII SacI SacII SexAI SfiI SrfI SwaI