(Circular) (Six-base) MAPSORT of: pFN21ASDB0185.seq Check: 2082 from: 1 to: 9667 pFN21ASDB0185 9667 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3190 6810 6939 3190 Size: 3620 129 5918 Fragments arranged by size: 5918 3620 129 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7477 278 Size: 53 83 186 936 5941 2468 Fragments arranged by size: 5941 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7010 1937 Size: 5073 4594 Acc65I G'GTAC_C Cuts at: 6988 6988 Size: 9667 AclI AA'CG_TT Cuts at: 1563 7794 8167 1563 Size: 6231 373 3063 Fragments arranged by size: 6231 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2912 3085 3109 3767 3795 3953 4607 4861 Size: 1280 173 24 658 28 158 654 254 Cuts at: 4861 4918 5819 7730 9114 9382 1632 Size: 57 901 1911 1384 268 1917 Fragments arranged by size: 1917 1911 1384 1280 901 658 654 268 254 173 158 57 28 24 AfeI AGC'GCT Cuts at: 4216 4216 Size: 9667 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9470 AflIII A'CryG_T Cuts at: 1203 2511 5589 8566 9543 1203 Size: 1308 3078 2977 977 1327 Fragments arranged by size: 3078 2977 1327 1308 977 AgeI A'CCGG_T Cuts at: 8498 8498 Size: 9667 AhdI GACnn_n'nnGTC Cuts at: 6469 8396 6469 Size: 1927 7740 AleI CACnn'nnGTG Cuts at: 1166 2937 3552 3995 1166 Size: 1771 615 443 6838 Fragments arranged by size: 6838 1771 615 443 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2945 2955 4013 6258 1184 Size: 1761 10 1058 2245 4593 Fragments arranged by size: 4593 2245 1761 1058 10 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5576 5608 6897 6929 5576 Size: 32 1289 32 8314 Fragments arranged by size: 8314 1289 32 32 AlwNI CAG_nnn'CTG Cuts at: 2215 2734 2925 3205 3793 4449 4639 4792 5003 Size: 519 191 280 588 656 190 153 211 Cuts at: 5003 5271 7410 8982 2215 Size: 268 2139 1572 2900 Fragments arranged by size: 2900 2139 1572 656 588 519 280 268 211 191 190 153 ApaLI G'TGCA_C Cuts at: 6589 7724 8880 6589 Size: 1135 1156 7376 Fragments arranged by size: 7376 1156 1135 ApoI r'AATT_y Cuts at: 1493 3636 6976 7202 7354 9463 1493 Size: 2143 3340 226 152 2109 1697 Fragments arranged by size: 3340 2143 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 4438 4438 Size: 9667 AseI AT'TA_AT Cuts at: 160 1032 8221 160 Size: 872 7189 1606 Fragments arranged by size: 7189 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9667 AvaI C'yCGr_G Cuts at: 1870 1943 2194 3377 3925 4766 5749 6528 6992 Size: 73 251 1183 548 841 983 779 464 Cuts at: 6992 1870 Size: 4545 Fragments arranged by size: 4545 1183 983 841 779 548 464 251 73 BamHI G'GATC_C Cuts at: 2133 6997 7364 2133 Size: 4864 367 4436 Fragments arranged by size: 4864 4436 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3554 4112 4642 4914 6028 Size: 325 217 627 1767 558 530 272 1114 Cuts at: 6028 6498 6988 8443 618 Size: 470 490 1455 1842 Fragments arranged by size: 1842 1767 1455 1114 627 558 530 490 470 325 272 217 BanII G_rGCy'C Cuts at: 1391 2457 2919 3921 4507 4551 4698 4839 6006 Size: 1066 462 1002 586 44 147 141 1167 Cuts at: 6006 6529 6986 1391 Size: 523 457 4072 Fragments arranged by size: 4072 1167 1066 1002 586 523 462 457 147 141 44 BbeI G_GCGC'C Cuts at: 6502 6502 Size: 9667 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2416 3259 3372 3569 4167 4345 6119 Size: 971 517 843 113 197 598 178 1774 Cuts at: 6119 6904 928 Size: 785 3691 Fragments arranged by size: 3691 1774 971 843 785 598 517 197 178 113 BbvCI CC'TCA_GC Cuts at: 3291 4715 4824 6818 3291 Size: 1424 109 1994 6140 Fragments arranged by size: 6140 1994 1424 109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4729 4763 5339 5373 6409 6443 7858 Size: 34 2792 34 576 34 1036 34 1415 Cuts at: 7858 7892 1903 Size: 34 3678 Fragments arranged by size: 3678 2792 1415 1036 576 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7559 8769 7559 Size: 1210 8457 BclI T'GATC_A Cuts at: 1548 2328 1548 Size: 780 8887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4055 4673 5102 Size: 107 122 71 631 605 2383 618 429 Cuts at: 5102 5225 5777 6093 8278 136 Size: 123 552 316 2185 1525 Fragments arranged by size: 2383 2185 1525 631 618 605 552 429 316 123 122 107 71 BglII A'GATC_T Cuts at: 6894 9662 6894 Size: 2768 6899 BlpI GC'TnA_GC Cuts at: 2828 2918 6416 7084 2828 Size: 90 3498 668 5411 Fragments arranged by size: 5411 3498 668 90 Bme1580I G_kGCm'C Cuts at: 1790 2178 2323 3683 3773 4194 4755 6033 6593 Size: 388 145 1360 90 421 561 1278 560 Cuts at: 6593 7728 8884 1790 Size: 1135 1156 2573 Fragments arranged by size: 2573 1360 1278 1156 1135 561 560 421 388 145 90 BmgBI CAC'GTC Cuts at: 1318 3761 5353 1318 Size: 2443 1592 5632 Fragments arranged by size: 5632 2443 1592 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4459 4554 5310 Size: 416 53 83 399 24 3042 95 756 Cuts at: 5310 7398 8356 442 Size: 2088 958 1753 Fragments arranged by size: 3042 2088 1753 958 756 416 399 95 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9667 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3940 3940 Size: 9667 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2956 3830 3928 4226 4307 4880 4980 5761 Size: 1717 874 98 298 81 573 100 781 Cuts at: 5761 6380 6740 6866 8327 1239 Size: 619 360 126 1461 2579 Fragments arranged by size: 2579 1717 1461 874 781 619 573 360 298 126 100 98 81 Bpu10I CC'TnA_GC Cuts at: 2033 3291 3842 4715 4824 6309 6818 6917 2033 Size: 1258 551 873 109 1485 509 99 4783 Fragments arranged by size: 4783 1485 1258 873 551 509 109 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4082 6635 7792 8657 8955 4082 Size: 2553 1157 865 298 4794 Fragments arranged by size: 4794 2553 1157 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3147 3492 3596 4032 4277 5074 5816 Size: 617 1648 345 104 436 245 797 742 Cuts at: 5816 8330 882 Size: 2514 2219 Fragments arranged by size: 2514 2219 1648 797 742 617 436 345 245 104 BsaAI yAC'GTr Cuts at: 493 1204 4189 5260 5590 6964 7458 493 Size: 711 2985 1071 330 1374 494 2702 Fragments arranged by size: 2985 2702 1374 1071 711 494 330 BsaBI GATnn'nnATC Cuts at: 2138 3440 9661 2138 Size: 1302 6221 2144 Fragments arranged by size: 6221 2144 1302 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3936 6499 7474 7856 Size: 53 83 186 936 2403 2563 975 382 Cuts at: 7856 8491 275 Size: 635 1451 Fragments arranged by size: 2563 2403 1451 975 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3660 5865 8100 8498 8772 8919 9330 3660 Size: 2205 2235 398 274 147 411 3997 Fragments arranged by size: 3997 2235 2205 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2221 2251 3803 3833 3926 Size: 30 165 30 818 30 1552 30 93 Cuts at: 3926 3956 4058 4088 5244 5274 5285 5315 5657 Size: 30 102 30 1156 30 11 30 342 Cuts at: 5657 5687 6038 6068 6866 6896 1178 Size: 30 351 30 798 30 3949 Fragments arranged by size: 3949 1552 1156 818 798 351 342 165 102 93 30 30 30 30 30 30 30 30 30 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2042 2960 4529 4532 4678 4877 5665 Size: 853 3 918 1569 3 146 199 788 Cuts at: 5665 5884 6158 6587 6737 6965 1186 Size: 219 274 429 150 228 3888 Fragments arranged by size: 3888 1569 918 853 788 429 274 228 219 199 150 146 3 3 BseYI C'CCAG_C Cuts at: 3205 3541 3741 4033 4170 4217 4306 4776 4780 Size: 336 200 292 137 47 89 470 4 Cuts at: 4780 5082 5415 5548 5960 6091 6642 6822 8870 Size: 302 333 133 412 131 551 180 2048 Cuts at: 8870 9366 3205 Size: 496 3506 Fragments arranged by size: 3506 2048 551 496 470 412 336 333 302 292 200 180 137 133 131 89 47 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2684 2891 2915 3779 4541 5243 5258 Size: 573 948 207 24 864 762 702 15 Cuts at: 5258 5653 6086 6632 6713 1163 Size: 395 433 546 81 4117 Fragments arranged by size: 4117 948 864 762 702 573 546 433 395 207 81 24 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3492 4959 5761 7117 7878 8906 Size: 275 186 1491 1467 802 1356 761 1028 Cuts at: 8906 1540 Size: 2301 Fragments arranged by size: 2301 1491 1467 1356 1028 802 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2433 3063 3234 3726 4256 4698 4851 6006 Size: 399 630 171 492 530 442 153 1155 Cuts at: 6006 6422 6593 6780 7728 7813 8884 2034 Size: 416 171 187 948 85 1071 2817 Fragments arranged by size: 2817 1155 1071 948 630 530 492 442 416 399 187 171 171 153 85 BsmI GAATG_Cn' Cuts at: 5174 7178 7271 5174 Size: 2004 93 7570 Fragments arranged by size: 7570 2004 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5648 5648 Size: 9667 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2178 2323 2433 2457 2919 3063 Size: 399 244 144 145 110 24 462 144 Cuts at: 3063 3234 3683 3726 3773 3921 4194 4256 4507 Size: 171 449 43 47 148 273 62 251 Cuts at: 4507 4551 4698 4755 4839 4851 6006 6033 6422 Size: 44 147 57 84 12 1155 27 389 Cuts at: 6422 6529 6593 6780 6986 7728 7813 8884 1391 Size: 107 64 187 206 742 85 1071 2174 Fragments arranged by size: 2174 1155 1071 742 462 449 399 389 273 251 244 206 187 171 148 147 145 144 144 110 107 85 84 64 62 57 47 44 43 27 24 12 BspEI T'CCGG_A Cuts at: 3660 5865 9330 3660 Size: 2205 3465 3997 Fragments arranged by size: 3997 3465 2205 BspHI T'CATG_A Cuts at: 2988 7556 9286 2988 Size: 4568 1730 3369 Fragments arranged by size: 4568 3369 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3190 4038 4661 5166 5838 6810 6939 7022 Size: 2346 848 623 505 672 972 129 83 Cuts at: 7022 844 Size: 3489 Fragments arranged by size: 3489 2346 972 848 672 623 505 129 83 BsrBI CCG'CTC Cuts at: 1394 1823 3846 4912 7554 1394 Size: 429 2023 1066 2642 3507 Fragments arranged by size: 3507 2642 2023 1066 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2117 2853 4311 5145 8162 8336 65 Size: 1183 869 736 1458 834 3017 174 1396 Fragments arranged by size: 3017 1458 1396 1183 869 834 736 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4115 5760 6422 8311 8498 1700 Size: 110 2305 1645 662 1889 187 2869 Fragments arranged by size: 2869 2305 1889 1645 662 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9667 BssHII G'CGCG_C Cuts at: 1925 4438 1925 Size: 2513 7154 BssSI C'ACGA_G Cuts at: 2789 4430 5479 6522 7727 8739 2789 Size: 1641 1049 1043 1205 1012 3717 Fragments arranged by size: 3717 1641 1205 1049 1043 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3793 5856 6028 794 Size: 2999 2063 172 4433 Fragments arranged by size: 4433 2999 2063 172 BstBI TT'CG_AA Cuts at: 2007 8471 2007 Size: 6464 3203 BstEII G'GTnAC_C Cuts at: 1184 2740 3244 4853 5530 1184 Size: 1556 504 1609 677 5321 Fragments arranged by size: 5321 1609 1556 677 504 BstXI CCAn_nnnn'nTGG Cuts at: 2410 5008 5581 6379 6481 2410 Size: 2598 573 798 102 5596 Fragments arranged by size: 5596 2598 798 573 102 BstYI r'GATC_y Cuts at: 1972 2133 3657 3961 6894 6997 7364 7749 7766 Size: 161 1524 304 2933 103 367 385 17 Cuts at: 7766 9218 9304 9316 9662 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2933 1977 1524 1452 385 367 346 304 161 103 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5785 5916 5785 Size: 131 9536 BtgI C'CryG_G Cuts at: 513 1065 2022 2418 4341 4479 5169 5775 6042 Size: 552 957 396 1923 138 690 606 267 Cuts at: 6042 6489 6516 513 Size: 447 27 3664 Fragments arranged by size: 3664 1923 957 690 606 552 447 396 267 138 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2758 2959 3862 3871 4030 5497 Size: 683 56 1532 201 903 9 159 1467 Cuts at: 5497 6412 487 Size: 915 3742 Fragments arranged by size: 3742 1532 1467 915 903 683 201 159 56 9 BtsI GCAGTG_nn' Cuts at: 4079 4147 4558 4814 5001 5469 7183 7977 7997 Size: 68 411 256 187 468 1714 794 20 Cuts at: 7997 4079 Size: 5749 Fragments arranged by size: 5749 1714 794 468 411 256 187 68 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9667 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4453 4488 529 Size: 35 3889 35 5708 Fragments arranged by size: 5708 3889 35 35 DraI TTT'AAA Cuts at: 6971 6971 Size: 9667 DraIII CAC_nnn'GTG Cuts at: 4189 4189 Size: 9667 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8674 809 Size: 507 7358 1802 Fragments arranged by size: 7358 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2412 2658 2793 Size: 54 1425 50 275 18 582 246 135 Cuts at: 2793 3489 4056 4674 5010 5181 5262 5374 5466 Size: 696 567 618 336 171 81 112 92 Cuts at: 5466 5758 5778 6039 6600 7114 8003 9632 8 Size: 292 20 261 561 514 889 1629 43 Fragments arranged by size: 1629 1425 889 696 618 582 567 561 514 336 292 275 261 246 171 135 112 92 81 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 3489 5758 7114 1537 Size: 275 1677 2269 1356 4090 Fragments arranged by size: 4090 2269 1677 1356 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 2969 3005 3659 4703 5168 5816 7597 Size: 761 855 36 654 1044 465 648 1781 Cuts at: 7597 1353 Size: 3423 Fragments arranged by size: 3423 1781 1044 855 761 654 648 465 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2229 2640 5890 8254 8628 8774 Size: 1064 138 640 411 3250 2364 374 146 Cuts at: 8774 387 Size: 1280 Fragments arranged by size: 3250 2364 1280 1064 640 411 374 146 138 EcoICRI GAG'CTC Cuts at: 4696 6004 4696 Size: 1308 8359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2912 2956 3085 3109 3767 3795 3830 Size: 393 1280 44 129 24 658 28 35 Cuts at: 3830 3928 3953 4226 4307 4607 4861 4880 4918 Size: 98 25 273 81 300 254 19 38 Cuts at: 4918 4980 5761 5819 6380 6740 6866 7730 8327 Size: 62 781 58 561 360 126 864 597 Cuts at: 8327 9114 9382 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1280 864 787 781 658 597 561 393 360 300 273 268 254 129 126 98 81 62 58 44 38 35 28 25 24 19 EcoNI CCTnn'n_nnAGG Cuts at: 2241 3455 3518 4511 4589 5479 2241 Size: 1214 63 993 78 890 6429 Fragments arranged by size: 6429 1214 993 890 78 63 EcoO109I rG'GnC_Cy Cuts at: 2086 3421 3751 4083 4636 4770 5293 5943 6324 Size: 1335 330 332 553 134 523 650 381 Cuts at: 6324 6349 6583 2086 Size: 25 234 5170 Fragments arranged by size: 5170 1335 650 553 523 381 332 330 234 134 25 EcoRI G'AATT_C Cuts at: 6976 7354 6976 Size: 378 9289 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4373 4373 Size: 9667 FspI TGC'GCA Cuts at: 2315 2906 5543 8173 2315 Size: 591 2637 2630 3809 Fragments arranged by size: 3809 2637 2630 591 FspAI rTGC'GCAy Cuts at: 2906 5543 2906 Size: 2637 7030 HaeII r_GCGC'y Cuts at: 1678 2048 4218 5097 5748 6316 6502 6719 8814 Size: 370 2170 879 651 568 186 217 2095 Cuts at: 8814 1678 Size: 2531 Fragments arranged by size: 2531 2170 2095 879 651 568 370 217 186 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 3926 3958 4029 Size: 32 135 32 809 32 1513 32 71 Cuts at: 4029 4061 4494 4526 5285 5317 6038 6070 6455 Size: 32 433 32 759 32 721 32 385 Cuts at: 6455 6487 6866 6898 8308 8340 8382 8414 1373 Size: 32 379 32 1410 32 42 32 2626 Fragments arranged by size: 2626 1513 1410 809 759 721 433 385 379 135 71 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3172 4963 7011 7257 669 Size: 988 281 1234 1791 2048 246 3079 Fragments arranged by size: 3079 2048 1791 1234 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9667 HpaI GTT'AAC Cuts at: 7257 7257 Size: 9667 KasI G'GCGC_C Cuts at: 6498 6498 Size: 9667 KpnI G_GTAC'C Cuts at: 6992 6992 Size: 9667 MfeI C'AATT_G Cuts at: 7266 7266 Size: 9667 MluI A'CGCG_T Cuts at: 9543 9543 Size: 9667 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 4515 5097 6181 8781 8965 1305 Size: 840 2370 582 1084 2600 184 2007 Fragments arranged by size: 2600 2370 2007 1084 840 582 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2795 4058 4676 5183 5264 Size: 54 1425 343 963 1263 618 507 81 Cuts at: 5264 6602 10 Size: 1338 3075 Fragments arranged by size: 3075 1425 1338 1263 963 618 507 343 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2339 2703 2906 2937 3552 3995 Size: 648 861 312 364 203 31 615 443 Cuts at: 3995 4340 5537 5900 6479 6608 7625 7984 8143 Size: 345 1197 363 579 129 1017 359 159 Cuts at: 8143 518 Size: 2042 Fragments arranged by size: 2042 1197 1017 861 648 615 579 443 364 363 359 345 312 203 159 129 31 MspA1I CmG'CkG Cuts at: 1606 2041 2464 2722 2809 2925 3627 3745 3973 Size: 435 423 258 87 116 702 118 228 Cuts at: 3973 4174 4529 4605 4780 4792 5552 6025 6217 Size: 201 355 76 175 12 760 473 192 Cuts at: 6217 6669 6822 7083 7760 8908 9153 1606 Size: 452 153 261 677 1148 245 2120 Fragments arranged by size: 2120 1148 760 702 677 473 452 435 423 355 261 258 245 228 201 192 175 153 118 116 87 76 12 NaeI GCC'GGC Cuts at: 4117 4117 Size: 9667 NarI GG'CG_CC Cuts at: 6499 6499 Size: 9667 NcoI C'CATG_G Cuts at: 513 1065 2022 4341 4479 5169 5775 513 Size: 552 957 2319 138 690 606 4405 Fragments arranged by size: 4405 2319 957 690 606 552 138 NdeI CA'TA_TG Cuts at: 387 387 Size: 9667 NgoMIV G'CCGG_C Cuts at: 4115 4115 Size: 9667 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9667 NotI GC'GGCC_GC Cuts at: 7114 7114 Size: 9667 NspI r_CATG'y Cuts at: 1132 1229 2338 2515 2672 2905 5237 6371 7025 Size: 97 1109 177 157 233 2332 1134 654 Cuts at: 7025 8570 1132 Size: 1545 2229 Fragments arranged by size: 2332 2229 1545 1134 1109 654 233 177 157 97 PasI CC'CwG_GG Cuts at: 1348 3272 1348 Size: 1924 7743 PciI A'CATG_T Cuts at: 2511 8566 2511 Size: 6055 3612 PflMI CCAn_nnn'nTGG Cuts at: 1108 3649 4015 4449 5271 5617 6568 6787 1108 Size: 2541 366 434 822 346 951 219 3988 Fragments arranged by size: 3988 2541 951 822 434 366 346 219 PfoI T'CCnGG_A Cuts at: 2100 2208 2643 3944 6459 6765 2100 Size: 108 435 1301 2515 306 5002 Fragments arranged by size: 5002 2515 1301 435 306 108 PmeI GTTT'AAAC Cuts at: 6971 6971 Size: 9667 PmlI CAC'GTG Cuts at: 4189 5260 5590 4189 Size: 1071 330 8266 Fragments arranged by size: 8266 1071 330 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7736 7768 9249 9281 7736 Size: 32 1481 32 8122 Fragments arranged by size: 8122 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2086 3421 4083 4636 4770 5293 5943 2086 Size: 1335 662 553 134 523 650 5810 Fragments arranged by size: 5810 1335 662 650 553 523 134 PshAI GACnn'nnGTC Cuts at: 1613 4655 1613 Size: 3042 6625 PsiI TTA'TAA Cuts at: 7237 7237 Size: 9667 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2753 2785 2753 Size: 32 9635 PstI C_TGCA'G Cuts at: 830 2076 4074 4422 4821 5103 6936 7019 830 Size: 1246 1998 348 399 282 1833 83 3478 Fragments arranged by size: 3478 1998 1833 1246 399 348 282 83 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9667 PvuII CAG'CTG Cuts at: 2041 2722 2809 2925 3745 4174 4605 4780 4792 Size: 681 87 116 820 429 431 175 12 Cuts at: 4792 5552 6822 2041 Size: 760 1270 4886 Fragments arranged by size: 4886 1270 820 760 681 431 429 175 116 87 12 SacI G_AGCT'C Cuts at: 4698 6006 4698 Size: 1308 8359 SalI G'TCGA_C Cuts at: 1936 7009 1936 Size: 5073 4594 SapI GCTCTTCn'nnn_ Cuts at: 3005 3659 4703 5168 5816 3005 Size: 654 1044 465 648 6856 Fragments arranged by size: 6856 1044 654 648 465 SbfI CC_TGCA'GG Cuts at: 5103 7019 5103 Size: 1916 7751 ScaI AGT'ACT Cuts at: 3401 7915 9627 3401 Size: 4514 1712 3441 Fragments arranged by size: 4514 3441 1712 SexAI A'CCwGG_T Cuts at: 3248 3423 3248 Size: 175 9492 SfcI C'TryA_G Cuts at: 826 1045 2072 4070 4418 4817 5099 6932 7015 Size: 219 1027 1998 348 399 282 1833 83 Cuts at: 7015 8150 8831 9022 826 Size: 1135 681 191 1471 Fragments arranged by size: 1998 1833 1471 1135 1027 681 399 348 282 219 191 83 SfiI GGCCn_nnn'nGGCC Cuts at: 4673 5225 5777 4673 Size: 552 552 8563 Fragments arranged by size: 8563 552 552 SfoI GGC'GCC Cuts at: 6500 6500 Size: 9667 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9667 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9667 SmaI CCC'GGG Cuts at: 1872 2196 4768 5751 6994 1872 Size: 324 2572 983 1243 4545 Fragments arranged by size: 4545 2572 1243 983 324 SmlI C'TyrA_G Cuts at: 820 1017 1943 4097 6650 7771 8672 8934 820 Size: 197 926 2154 2553 1121 901 262 1553 Fragments arranged by size: 2553 2154 1553 1121 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6964 7458 493 Size: 6471 494 2702 Fragments arranged by size: 6471 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9667 SphI G_CATG'C Cuts at: 1132 2672 7025 1132 Size: 1540 4353 3774 Fragments arranged by size: 4353 3774 1540 SspI AAT'ATT Cuts at: 5 52 7591 5 Size: 47 7539 2081 Fragments arranged by size: 7539 2081 47 StuI AGG'CCT Cuts at: 1517 5345 6734 1517 Size: 3828 1389 4450 Fragments arranged by size: 4450 3828 1389 StyI C'CwwG_G Cuts at: 513 1065 2022 2600 3338 3456 3897 4059 4341 Size: 552 957 578 738 118 441 162 282 Cuts at: 4341 4479 4725 5109 5169 5775 6009 6687 6781 Size: 138 246 384 60 606 234 678 94 Cuts at: 6781 6855 7106 513 Size: 74 251 3074 Fragments arranged by size: 3074 957 738 678 606 578 552 441 384 282 251 246 234 162 138 118 94 74 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4022 7694 7711 7864 8049 8494 325 Size: 1255 2442 3672 17 153 185 445 1498 Fragments arranged by size: 3672 2442 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2898 3399 7913 Size: 274 80 33 51 1443 921 501 4514 Cuts at: 7913 9625 96 Size: 1712 138 Fragments arranged by size: 4514 1712 1443 921 501 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2221 2253 2552 2584 3803 3835 4058 4090 5244 Size: 32 299 32 1219 32 223 32 1154 Cuts at: 5244 5276 6379 6411 9627 9659 2221 Size: 32 1103 32 3216 32 2229 Fragments arranged by size: 3216 2229 1219 1154 1103 299 223 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9667 XbaI T'CTAG_A Cuts at: 7003 7003 Size: 9667 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2250 3928 4012 4201 4339 5176 5524 5614 2250 Size: 1678 84 189 138 837 348 90 6303 Fragments arranged by size: 6303 1678 837 348 189 138 90 84 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9667 XmaI C'CCGG_G Cuts at: 1870 2194 4766 5749 6992 1870 Size: 324 2572 983 1243 4545 Fragments arranged by size: 4545 2572 1243 983 324 XmnI GAAnn'nnTTC Cuts at: 5138 5450 6833 7796 5138 Size: 312 1383 963 7009 Fragments arranged by size: 7009 1383 963 312 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7475 276 Size: 53 83 186 936 5941 2468 Fragments arranged by size: 5941 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI NruI NsiI PacI PspOMI PspXI RsrII SacII SanDI SrfI SwaI