Restriction map for
pFN21ASDB0068
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# NO CUT ENZYMES ( 86 enzymes) #
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AccI AccIII AclI AflII AflIII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp700I AvrII BamHI BbrPI
BciVI BclI BfrI BglI BlnI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I EcoO65I EcoRI FbaI FseI
HpaI KspI MfeI MluI MroI
MunI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StuI SwaI XbaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 2410 GACGTC GACGT^C
Acc65I 2675 GGTACC G^GTACC
AccII 1227 CGCG CG^CG
AhdI 1495 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 266 CCTCAGG CC^T[ACGT]AGG
Asp718I 2675 GGTACC G^GTACC
AspEI 1495 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1220 TGCGCA TGC^GCA
BbeI 2653 GGCGCC GGCGC^C
BcgI 2393 GCAAGCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2224 CTAG C^TAG
BglII 2556 AGATCT A^GATCT
BsaAI 2543 TACGTG [CT]AC^GT[GA]
BsaBI 315 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstAPI 1338 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1227 CGCG CG^CG
Bsu36I 266 CCTCAGG CC^T[ACGT]AGG
Eam1105I 1495 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 266 CCTCAGG CC^T[ACGT]AGG
EcoNI 54 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3004 GATATC GAT^ATC
EcoT22I 191 ATGCAT ATGCA^T
FspI 1220 TGCGCA TGC^GCA
HgaI 116 GCGTC GACGC(5/10)
HincII 1008 GTTGAC GT[CT]^[GA]AC
HindII 1008 GTTGAC GT[CT]^[GA]AC
KasI 2653 GGCGCC G^GCGCC
KpnI 2675 GGTACC GGTAC^C
MaeI 2224 CTAG C^TAG
MamI 315 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1227 CGCG CG^CG
NaeI 1814 GCCGGC GCC^GGC
NarI 2653 GGCGCC GG^CGCC
NdeI 137 CATATG CA^TATG
NgoMIV 1814 GCCGGC G^CCGGC
NsiI 191 ATGCAT ATGCA^T
PaeR7I 2420 CTCGAG C^TCGAG
PshAI 2418 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2874 CCTGCAGG CCTGCA^GG
SfoI 2653 GGCGCC GGC^GCC
SmaI 2363 CCCGGG CCC^GGG
Sse8387I 2874 CCTGCAGG CCTGCA^GG
XcmI 1574 CCATGCTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2420 CTCGAG C^TCGAG
XmaCI 2363 CCCGGG C^CCGGG
XmaI 2363 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 2410 GACGTC G[GA]^CG[CT]C
2653 GGCGCC
Alw44I 466 GTGCAC G^TGCAC
2630 GTGCAC
ApaLI 466 GTGCAC G^TGCAC
2630 GTGCAC
AspI 112 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
939 GACCAAGTC
BsaHI 2410 GACGTC G[GA]^CG[CT]C
2653 GGCGCC
BsgI 298 CTGCAC GTGCAG(16/14)
2435 GTGCAG
BsmBI 1267 CGTCTC CGTCTC(1/5)
1072 GAGACG
BsrBI 1920 CCGCTC CCGCTC(-3/-3)
1237 GAGCGG
BsrDI 788 GCAATG GCAATG(2/0)
1112 GCAATG
BssSI 469 CACGAG CACGAG(-5/-1)
2269 CACGAG
DrdI 109 GACGACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
453 GACAAATCTGTC
DsaI 284 CCATGG C^C[GA][CT]GG
365 CCATGG
Hin1I 2410 GACGTC G[GA]^CG[CT]C
2653 GGCGCC
HindIII 2230 AAGCTT A^AGCTT
3061 AAGCTT
MaeII 2411 ACGT A^CGT
2544 ACGT
NcoI 284 CCATGG C^CATGG
365 CCATGG
NspI 193 GCATGT [GA]CATG^[CT]
1937 ACATGC
PflFI 112 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
939 GACCAAGTC
PflMI 1506 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1536 CCAGCTTCTGG
PpuMI 1466 AGGTCCT [GA]G^G[AT]CC[CT]
2333 AGGACCT
Tth111I 112 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
939 GACCAAGTC
Van91I 1506 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1536 CCAGCTTCTGG
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII