(Circular) (Six-base) MAPSORT of: pFN21ASDB0068.seq Check: 8132 from: 1 to: 7825 pFN21ASDB0068 7825 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3447 3447 Size: 7825 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4437 5635 278 Size: 53 83 186 936 2901 1198 2468 Fragments arranged by size: 2901 2468 1198 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5168 1937 Size: 3231 4594 Acc65I G'GTAC_C Cuts at: 4698 5146 4698 Size: 448 7377 AclI AA'CG_TT Cuts at: 1563 5952 6325 1563 Size: 4389 373 3063 Fragments arranged by size: 4389 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4088 4241 4298 4679 5888 7272 7540 1632 Size: 2456 153 57 381 1209 1384 268 1917 Fragments arranged by size: 2456 1917 1384 1209 381 268 153 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7628 AflIII A'CryG_T Cuts at: 1203 6724 7701 1203 Size: 5521 977 1327 Fragments arranged by size: 5521 1327 977 AgeI A'CCGG_T Cuts at: 6656 6656 Size: 7825 AhdI GACnn_n'nnGTC Cuts at: 3523 6554 3523 Size: 3031 4794 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7825 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2072 4325 1184 Size: 888 2253 4684 Fragments arranged by size: 4684 2253 888 AlwNI CAG_nnn'CTG Cuts at: 3493 3565 4318 5568 7140 3493 Size: 72 753 1250 1572 4178 Fragments arranged by size: 4178 1572 1250 753 72 ApaLI G'TGCA_C Cuts at: 2489 4653 5882 7038 2489 Size: 2164 1229 1156 3276 Fragments arranged by size: 3276 2164 1229 1156 ApoI r'AATT_y Cuts at: 1493 2433 2448 3022 5134 5360 5512 7621 1493 Size: 940 15 574 2112 226 152 2109 1697 Fragments arranged by size: 2112 2109 1697 940 574 226 152 15 AseI AT'TA_AT Cuts at: 160 1032 6379 160 Size: 872 5347 1606 Fragments arranged by size: 5347 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7825 AvaI C'yCGr_G Cuts at: 1870 1943 2361 2897 3254 3399 4386 4443 4747 Size: 73 418 536 357 145 987 57 304 Cuts at: 4747 5150 1870 Size: 403 4545 Fragments arranged by size: 4545 987 536 418 403 357 304 145 73 57 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4930 4963 4930 Size: 33 7792 BamHI G'GATC_C Cuts at: 5155 5522 5155 Size: 367 7458 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2261 2509 3834 3987 4676 Size: 325 217 627 474 248 1325 153 689 Cuts at: 4676 4698 4832 5146 6601 618 Size: 22 134 314 1455 1842 Fragments arranged by size: 1842 1455 1325 689 627 474 325 314 248 217 153 134 22 BanII G_rGCy'C Cuts at: 1391 3201 3900 4215 4473 4748 4844 5144 1391 Size: 1810 699 315 258 275 96 300 4072 Fragments arranged by size: 4072 1810 699 315 300 275 258 96 BbeI G_GCGC'C Cuts at: 4680 4680 Size: 7825 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3083 4205 4482 928 Size: 971 1184 1122 277 4271 Fragments arranged by size: 4271 1184 1122 971 277 BbvCI CC'TCA_GC Cuts at: 2331 2832 3666 5070 2331 Size: 501 834 1404 5086 Fragments arranged by size: 5086 1404 834 501 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4405 4439 6016 6050 1903 Size: 34 2468 34 1577 34 3678 Fragments arranged by size: 3678 2468 1577 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5717 6927 5717 Size: 1210 6615 BclI T'GATC_A Cuts at: 1548 1548 Size: 7825 BfrBI ATG'CAT Cuts at: 2216 2216 Size: 7825 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6436 136 Size: 107 122 71 631 605 4764 1525 Fragments arranged by size: 4764 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4579 7820 4579 Size: 3241 4584 BlpI GC'TnA_GC Cuts at: 3039 3117 3122 3431 5242 3039 Size: 78 5 309 1811 5622 Fragments arranged by size: 5622 1811 309 78 5 Bme1580I G_kGCm'C Cuts at: 1790 2493 2840 4657 5886 7042 1790 Size: 703 347 1817 1229 1156 2573 Fragments arranged by size: 2573 1817 1229 1156 703 347 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7825 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5556 6514 442 Size: 416 53 83 399 24 4139 958 1753 Fragments arranged by size: 4139 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7825 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3215 3215 Size: 7825 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2769 2870 3131 3515 3629 3839 3866 4019 Size: 1530 101 261 384 114 210 27 153 Cuts at: 4019 4139 4220 4379 4391 4667 6485 1239 Size: 120 81 159 12 276 1818 2579 Fragments arranged by size: 2579 1818 1530 384 276 261 210 159 153 120 114 101 81 27 12 Bpu10I CC'TnA_GC Cuts at: 2331 2832 3462 3666 5070 2331 Size: 501 630 204 1404 5086 Fragments arranged by size: 5086 1404 630 501 204 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2181 2423 3341 4033 4346 4541 5950 6815 7113 Size: 242 918 692 313 195 1409 865 298 Cuts at: 7113 2181 Size: 2893 Fragments arranged by size: 2893 1409 918 865 692 313 298 242 195 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2024 2954 3203 6488 882 Size: 617 525 930 249 3285 2219 Fragments arranged by size: 3285 2219 930 617 525 249 BsaAI yAC'GTr Cuts at: 493 1204 4568 5122 5616 493 Size: 711 3364 554 494 2702 Fragments arranged by size: 3364 2702 711 554 494 BsaBI GATnn'nnATC Cuts at: 2342 7819 2342 Size: 5477 2348 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4434 4677 5632 6014 Size: 53 83 186 936 2901 243 955 382 Cuts at: 6014 6649 275 Size: 635 1451 Fragments arranged by size: 2901 1451 955 936 635 382 243 186 83 53 BsaWI w'CCGG_w Cuts at: 6258 6656 6930 7077 7488 6258 Size: 398 274 147 411 6595 Fragments arranged by size: 6595 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2369 2399 3132 3162 3602 Size: 30 165 30 966 30 733 30 440 Cuts at: 3602 3632 1178 Size: 30 5371 Fragments arranged by size: 5371 966 733 440 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2570 3191 3221 3272 3290 3293 3417 3512 Size: 1384 621 30 51 18 3 124 95 Cuts at: 3512 3554 3779 3782 3905 4388 1186 Size: 42 225 3 123 483 4623 Fragments arranged by size: 4623 1384 621 483 225 124 123 95 51 42 30 18 3 3 BseYI C'CCAG_C Cuts at: 3170 4804 4910 7028 7524 3170 Size: 1634 106 2118 496 3471 Fragments arranged by size: 3471 2118 1634 496 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 4479 1163 Size: 573 570 2173 4509 Fragments arranged by size: 4509 2173 573 570 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5275 6036 7064 1540 Size: 275 186 3274 761 1028 2301 Fragments arranged by size: 3274 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 2493 3201 3900 4215 4304 4657 4748 5886 Size: 432 708 699 315 89 353 91 1138 Cuts at: 5886 5971 7042 2061 Size: 85 1071 2844 Fragments arranged by size: 2844 1138 1071 708 699 432 353 315 91 89 85 BsmI GAATG_Cn' Cuts at: 5336 5429 5336 Size: 93 7732 BsmBI CGTCTCn'nnnn_ Cuts at: 3089 3296 3089 Size: 207 7618 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2493 2840 3201 3900 4215 4304 Size: 399 271 432 347 361 699 315 89 Cuts at: 4304 4473 4657 4748 4844 5144 5886 5971 7042 Size: 169 184 91 96 300 742 85 1071 Cuts at: 7042 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 699 432 399 361 347 315 300 271 184 169 96 91 89 85 BspEI T'CCGG_A Cuts at: 7488 7488 Size: 7825 BspHI T'CATG_A Cuts at: 5714 7444 5714 Size: 1730 6095 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3447 3478 4905 5058 5180 844 Size: 2603 31 1427 153 122 3489 Fragments arranged by size: 3489 2603 1427 153 122 31 BsrBI CCG'CTC Cuts at: 1394 1823 3262 3945 5712 1394 Size: 429 1439 683 1767 3507 Fragments arranged by size: 3507 1767 1439 683 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2818 3142 6320 6494 65 Size: 1183 1570 324 3178 174 1396 Fragments arranged by size: 3178 1570 1396 1183 324 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3126 3837 4155 4695 6469 6656 1700 Size: 110 1316 711 318 540 1774 187 2869 Fragments arranged by size: 2869 1774 1316 711 540 318 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7825 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7825 BssSI C'ACGA_G Cuts at: 2492 4292 5885 6897 2492 Size: 1800 1593 1012 3420 Fragments arranged by size: 3420 1800 1593 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3367 794 Size: 2573 5252 BstBI TT'CG_AA Cuts at: 2007 6629 2007 Size: 4622 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7825 BstYI r'GATC_y Cuts at: 1972 3282 3691 3939 4579 5155 5522 5907 5924 Size: 1310 409 248 640 576 367 385 17 Cuts at: 5924 7376 7462 7474 7820 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1310 640 576 409 385 367 346 248 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2290 2290 Size: 7825 BtgI C'CryG_G Cuts at: 513 1065 2022 2307 2388 513 Size: 552 957 285 81 5950 Fragments arranged by size: 5950 957 552 285 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3232 4032 487 Size: 683 56 2006 800 4280 Fragments arranged by size: 4280 2006 800 683 56 BtsI GCAGTG_nn' Cuts at: 3009 3050 4960 5341 6135 6155 3009 Size: 41 1910 381 794 20 4679 Fragments arranged by size: 4679 1910 794 381 41 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7825 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7790 DraI TTT'AAA Cuts at: 5129 5129 Size: 7825 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2138 2482 6832 809 Size: 507 822 344 4350 1802 Fragments arranged by size: 4350 1802 822 507 344 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3591 4026 4795 Size: 54 1425 50 275 18 1761 435 769 Cuts at: 4795 5272 6161 7790 8 Size: 477 889 1629 43 Fragments arranged by size: 1761 1629 1425 889 769 477 435 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5272 1537 Size: 275 3460 4090 Fragments arranged by size: 4090 3460 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3375 3539 3990 4274 4292 4877 5755 1353 Size: 2022 164 451 284 18 585 878 3423 Fragments arranged by size: 3423 2022 878 585 451 284 164 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 3275 6412 6786 6932 387 Size: 1064 138 453 1233 3137 374 146 1280 Fragments arranged by size: 3137 1280 1233 1064 453 374 146 138 EcoICRI GAG'CTC Cuts at: 3199 3898 4213 4746 3199 Size: 699 315 533 6278 Fragments arranged by size: 6278 699 533 315 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2769 2870 3131 3515 3629 3839 3866 Size: 393 1137 101 261 384 114 210 27 Cuts at: 3866 4019 4088 4139 4220 4241 4298 4379 4391 Size: 153 69 51 81 21 57 81 12 Cuts at: 4391 4667 4679 5888 6485 7272 7540 1239 Size: 276 12 1209 597 787 268 1524 Fragments arranged by size: 1524 1209 1137 787 597 393 384 276 268 261 210 153 114 101 81 81 69 57 51 27 21 12 12 EcoNI CCTnn'n_nnAGG Cuts at: 2081 2081 Size: 7825 EcoO109I rG'GnC_Cy Cuts at: 3490 4357 4642 3490 Size: 867 285 6673 Fragments arranged by size: 6673 867 285 EcoRI G'AATT_C Cuts at: 5134 5512 5134 Size: 378 7447 EcoRV GAT'ATC Cuts at: 5029 5029 Size: 7825 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2261 4531 2261 Size: 2270 5555 FspI TGC'GCA Cuts at: 3245 6331 3245 Size: 3086 4739 FspAI rTGC'GCAy Cuts at: 3245 3245 Size: 7825 HaeII r_GCGC'y Cuts at: 1678 3166 4680 4696 6972 1678 Size: 1488 1514 16 2276 2531 Fragments arranged by size: 2531 2276 1514 1488 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2951 2983 3215 3247 3274 Size: 32 135 32 1379 32 232 32 27 Cuts at: 3274 3306 3509 3541 3563 3595 3676 3708 3777 Size: 32 203 32 22 32 81 32 69 Cuts at: 3777 3809 3887 3919 4120 4152 6466 6498 6540 Size: 32 78 32 201 32 2314 32 42 Cuts at: 6540 6572 1373 Size: 32 2626 Fragments arranged by size: 2626 2314 1379 232 203 201 135 81 78 69 42 32 32 32 32 32 32 32 32 32 32 32 32 32 27 22 HincII GTy'rAC Cuts at: 669 1657 1938 3033 5169 5415 669 Size: 988 281 1095 2136 246 3079 Fragments arranged by size: 3079 2136 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 4253 5084 748 Size: 3505 831 3489 Fragments arranged by size: 3505 3489 831 HpaI GTT'AAC Cuts at: 5415 5415 Size: 7825 KasI G'GCGC_C Cuts at: 4676 4676 Size: 7825 KpnI G_GTAC'C Cuts at: 4702 5150 4702 Size: 448 7377 MfeI C'AATT_G Cuts at: 5424 5424 Size: 7825 MluI A'CGCG_T Cuts at: 7701 7701 Size: 7825 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6939 7123 1305 Size: 5634 184 2007 Fragments arranged by size: 5634 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3593 4028 4797 10 Size: 54 1425 343 1761 435 769 3038 Fragments arranged by size: 3038 1761 1425 769 435 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 2217 2612 2975 3239 5783 6142 Size: 648 888 163 395 363 264 2544 359 Cuts at: 6142 6301 518 Size: 159 2042 Fragments arranged by size: 2544 2042 888 648 395 363 359 264 163 159 MspA1I CmG'CkG Cuts at: 1606 2335 2989 3424 3625 3844 4162 4561 5241 Size: 729 654 435 201 219 318 399 680 Cuts at: 5241 5918 7066 7311 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 729 680 677 654 435 399 318 245 219 201 NaeI GCC'GGC Cuts at: 3839 3839 Size: 7825 NarI GG'CG_CC Cuts at: 4677 4677 Size: 7825 NcoI C'CATG_G Cuts at: 513 1065 2022 2307 2388 513 Size: 552 957 285 81 5950 Fragments arranged by size: 5950 957 552 285 81 NdeI CA'TA_TG Cuts at: 387 2161 387 Size: 1774 6051 NgoMIV G'CCGG_C Cuts at: 3837 3837 Size: 7825 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7825 NotI GC'GGCC_GC Cuts at: 5272 5272 Size: 7825 NsiI A_TGCA'T Cuts at: 2218 2218 Size: 7825 NspI r_CATG'y Cuts at: 1132 1229 2220 3964 5183 6728 1132 Size: 97 991 1744 1219 1545 2229 Fragments arranged by size: 2229 1744 1545 1219 991 97 PasI CC'CwG_GG Cuts at: 1348 3187 1348 Size: 1839 5986 PciI A'CATG_T Cuts at: 6724 6724 Size: 7825 PflMI CCAn_nnn'nTGG Cuts at: 1108 3535 3565 1108 Size: 2427 30 5368 Fragments arranged by size: 5368 2427 30 PfoI T'CCnGG_A Cuts at: 2886 3492 4173 2886 Size: 606 681 6538 Fragments arranged by size: 6538 681 606 PmeI GTTT'AAAC Cuts at: 5129 5129 Size: 7825 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2999 3031 3755 3787 5894 5926 7407 7439 2999 Size: 32 724 32 2107 32 1481 32 3385 Fragments arranged by size: 3385 2107 1481 724 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3490 4357 3490 Size: 867 6958 PshAI GACnn'nnGTC Cuts at: 1613 4445 1613 Size: 2832 4993 PsiI TTA'TAA Cuts at: 2949 5395 2949 Size: 2446 5379 PspXI vC'TCGA_Gb Cuts at: 4443 4443 Size: 7825 PstI C_TGCA'G Cuts at: 830 3489 4128 4314 4902 5177 830 Size: 2659 639 186 588 275 3478 Fragments arranged by size: 3478 2659 639 588 275 186 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7825 PvuII CAG'CTG Cuts at: 2335 3844 4162 4561 2335 Size: 1509 318 399 5599 Fragments arranged by size: 5599 1509 399 318 SacI G_AGCT'C Cuts at: 3201 3900 4215 4748 3201 Size: 699 315 533 6278 Fragments arranged by size: 6278 699 533 315 SalI G'TCGA_C Cuts at: 1936 5167 1936 Size: 3231 4594 SapI GCTCTTCn'nnn_ Cuts at: 3375 3539 4274 4292 3375 Size: 164 735 18 6908 Fragments arranged by size: 6908 735 164 18 SbfI CC_TGCA'GG Cuts at: 4902 5177 4902 Size: 275 7550 ScaI AGT'ACT Cuts at: 6073 7785 6073 Size: 1712 6113 SfcI C'TryA_G Cuts at: 826 1045 3485 3629 4124 4310 4898 5173 6308 Size: 219 2440 144 495 186 588 275 1135 Cuts at: 6308 6989 7180 826 Size: 681 191 1471 Fragments arranged by size: 2440 1471 1135 681 588 495 275 219 191 186 144 SfoI GGC'GCC Cuts at: 4678 4678 Size: 7825 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7825 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7825 SmaI CCC'GGG Cuts at: 1872 4388 5152 1872 Size: 2516 764 4545 Fragments arranged by size: 4545 2516 764 SmlI C'TyrA_G Cuts at: 820 1017 1943 2196 2402 3356 4012 4361 4443 Size: 197 926 253 206 954 656 349 82 Cuts at: 4443 4520 5929 6830 7092 820 Size: 77 1409 901 262 1553 Fragments arranged by size: 1553 1409 954 926 901 656 349 262 253 206 197 82 77 SnaBI TAC'GTA Cuts at: 493 5122 5616 493 Size: 4629 494 2702 Fragments arranged by size: 4629 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7825 SphI G_CATG'C Cuts at: 1132 5183 1132 Size: 4051 3774 SspI AAT'ATT Cuts at: 5 52 5749 5 Size: 47 5697 2081 Fragments arranged by size: 5697 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7825 StyI C'CwwG_G Cuts at: 513 1065 2022 2125 2307 2388 4668 4980 5264 Size: 552 957 103 182 81 2280 312 284 Cuts at: 5264 513 Size: 3074 Fragments arranged by size: 3074 2280 957 552 312 284 182 103 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4831 4924 5852 5869 6022 6207 6652 Size: 1255 3251 93 928 17 153 185 445 Cuts at: 6652 325 Size: 1498 Fragments arranged by size: 3251 1498 1255 928 445 185 153 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2528 4929 6071 Size: 274 80 33 51 1443 551 2401 1142 Cuts at: 6071 7783 96 Size: 1712 138 Fragments arranged by size: 2401 1712 1443 1142 551 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2369 2401 3132 3164 7785 7817 2369 Size: 32 731 32 4621 32 2377 Fragments arranged by size: 4621 2377 731 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2138 2965 1316 Size: 822 827 6176 Fragments arranged by size: 6176 827 822 XbaI T'CTAG_A Cuts at: 5161 5161 Size: 7825 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3604 3604 Size: 7825 XhoI C'TCGA_G Cuts at: 1943 4443 1943 Size: 2500 5325 XmaI C'CCGG_G Cuts at: 1870 4386 5150 1870 Size: 2516 764 4545 Fragments arranged by size: 4545 2516 764 XmnI GAAnn'nnTTC Cuts at: 5954 5954 Size: 7825 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4435 5633 276 Size: 53 83 186 936 2901 1198 2468 Fragments arranged by size: 2901 2468 1198 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BsiWI BstXI BstZ17I DraIII FseI NruI PacI PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI