Restriction map for
pFN21ASDB0068
(Linear) (Six-base) MAPSORT of: KIBB0068.seq Check: 2808 from: 1 to: 3096
KIBB0068.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1424 3096
Size: 1424 1672
AatII G_ACGT'C
Cuts at: 0 2414 3096
Size: 2414 682
Acc65I G'GTAC_C
Cuts at: 0 2675 3096
Size: 2675 421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2065 2218 2275 2656 3096
Size: 2065 153 57 381 440
Fragments arranged by size:
2065 440 381 153 57
AhdI GACnn_n'nnGTC
Cuts at: 0 1500 3096
Size: 1500 1596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 49 2302 3096
Size: 49 2253 794
Fragments arranged by size:
2253 794 49
AlwNI CAG_nnn'CTG
Cuts at: 0 1470 1542 2295 3096
Size: 1470 72 753 801
Fragments arranged by size:
1470 801 753 72
ApaLI G'TGCA_C
Cuts at: 0 466 2630 3096
Size: 466 2164 466
Fragments arranged by size:
2164 466 466
ApoI r'AATT_y
Cuts at: 0 410 425 999 3096
Size: 410 15 574 2097
Fragments arranged by size:
2097 574 410 15
AvaI C'yCGr_G
Cuts at: 0 338 874 1231 1376 2363 2420 2724 3096
Size: 338 536 357 145 987 57 304 372
Fragments arranged by size:
987 536 372 357 338 304 145 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2907 2940 3096
Size: 2907 33 156
Fragments arranged by size:
2907 156 33
BanI G'GyrC_C
Cuts at: 0 238 486 1811 1964 2653 2675 2809 3096
Size: 238 248 1325 153 689 22 134 287
Fragments arranged by size:
1325 689 287 248 238 153 134 22
BanII G_rGCy'C
Cuts at: 0 1178 1877 2192 2450 2725 2821 3096
Size: 1178 699 315 258 275 96 275
Fragments arranged by size:
1178 699 315 275 275 258 96
BbeI G_GCGC'C
Cuts at: 0 2657 3096
Size: 2657 439
BbsI GAAGACnn'nnnn_
Cuts at: 0 1060 2182 2459 3096
Size: 1060 1122 277 637
Fragments arranged by size:
1122 1060 637 277
BbvCI CC'TCA_GC
Cuts at: 0 308 809 1643 3047 3096
Size: 308 501 834 1404 49
Fragments arranged by size:
1404 834 501 308 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2382 2416 3096
Size: 2382 34 680
Fragments arranged by size:
2382 680 34
BfrBI ATG'CAT
Cuts at: 0 193 3096
Size: 193 2903
BglII A'GATC_T
Cuts at: 0 2556 3096
Size: 2556 540
BlpI GC'TnA_GC
Cuts at: 0 1016 1094 1099 1408 3096
Size: 1016 78 5 309 1688
Fragments arranged by size:
1688 1016 309 78 5
Bme1580I G_kGCm'C
Cuts at: 0 470 817 2634 3096
Size: 470 347 1817 462
Fragments arranged by size:
1817 470 462 347
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1192 3096
Size: 1192 1904
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 847 1108 1492 1606 1816 1843 1996
Size: 746 101 261 384 114 210 27 153
Cuts at: 1996 2116 2197 2356 2368 2644 3096
Size: 120 81 159 12 276 452
Fragments arranged by size:
746 452 384 276 261 210 159 153
120 114 101 81 27 12
Bpu10I CC'TnA_GC
Cuts at: 0 308 809 1439 1643 3047 3096
Size: 308 501 630 204 1404 49
Fragments arranged by size:
1404 630 501 308 204 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 400 1318 2010 2323 2518 3096
Size: 158 242 918 692 313 195 578
Fragments arranged by size:
918 692 578 313 242 195 158
BsaI GGTCTCn'nnnn_
Cuts at: 0 1 931 1180 3096
Size: 1 930 249 1916
Fragments arranged by size:
1916 930 249 1
BsaAI yAC'GTr
Cuts at: 0 2545 3096
Size: 2545 551
BsaBI GATnn'nnATC
Cuts at: 0 319 3096
Size: 319 2777
BsaHI Gr'CG_yC
Cuts at: 0 2411 2654 3096
Size: 2411 243 442
Fragments arranged by size:
2411 442 243
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 346 376 1109 1139 1579 1609 3096
Size: 346 30 733 30 440 30 1487
Fragments arranged by size:
1487 733 440 346 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 547 1168 1198 1249 1267 1270 1394 1489
Size: 547 621 30 51 18 3 124 95
Cuts at: 1489 1531 1756 1759 1882 2365 3096
Size: 42 225 3 123 483 731
Fragments arranged by size:
731 621 547 483 225 124 123 95
51 42 30 18 3 3
BseYI C'CCAG_C
Cuts at: 0 1147 2781 2887 3096
Size: 1147 1634 106 209
Fragments arranged by size:
1634 1147 209 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 2456 3096
Size: 283 2173 640
Fragments arranged by size:
2173 640 283
BsiHKAI G_wGCw'C
Cuts at: 0 38 470 1178 1877 2192 2281 2634 2725
Size: 38 432 708 699 315 89 353 91
Cuts at: 2725 3096
Size: 371
Fragments arranged by size:
708 699 432 371 353 315 91 89
38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1066 1273 3096
Size: 1066 207 1823
Fragments arranged by size:
1823 1066 207
Bsp1286I G_dGCh'C
Cuts at: 0 38 470 817 1178 1877 2192 2281 2450
Size: 38 432 347 361 699 315 89 169
Cuts at: 2450 2634 2725 2821 3096
Size: 184 91 96 275
Fragments arranged by size:
699 432 361 347 315 275 184 169
96 91 89 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1424 1455 2882 3035 3096
Size: 1424 31 1427 153 61
Fragments arranged by size:
1427 1424 153 61 31
BsrBI CCG'CTC
Cuts at: 0 1239 1922 3096
Size: 1239 683 1174
Fragments arranged by size:
1239 1174 683
BsrDI GCAATG_nn'
Cuts at: 0 795 1119 3096
Size: 795 324 1977
Fragments arranged by size:
1977 795 324
BsrFI r'CCGG_y
Cuts at: 0 1103 1814 2132 2672 3096
Size: 1103 711 318 540 424
Fragments arranged by size:
1103 711 540 424 318
BssSI C'ACGA_G
Cuts at: 0 469 2269 3096
Size: 469 1800 827
Fragments arranged by size:
1800 827 469
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1344 3096
Size: 1344 1752
BstYI r'GATC_y
Cuts at: 0 1259 1668 1916 2556 3096
Size: 1259 409 248 640 540
Fragments arranged by size:
1259 640 540 409 248
Bsu36I CC'TnA_GG
Cuts at: 0 267 3096
Size: 267 2829
BtgI C'CryG_G
Cuts at: 0 284 365 3096
Size: 284 81 2731
Fragments arranged by size:
2731 284 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1209 2009 3096
Size: 1209 800 1087
Fragments arranged by size:
1209 1087 800
BtsI GCAGTG_nn'
Cuts at: 0 986 1027 2937 3096
Size: 986 41 1910 159
Fragments arranged by size:
1910 986 159 41
DrdI GACnn_nn'nnGTC
Cuts at: 0 115 459 3096
Size: 115 344 2637
Fragments arranged by size:
2637 344 115
EaeI y'GGCC_r
Cuts at: 0 1568 2003 2772 3096
Size: 1568 435 769 324
Fragments arranged by size:
1568 769 435 324
EarI CTCTTCn'nnn_
Cuts at: 0 1352 1516 1967 2251 2269 2854 3096
Size: 1352 164 451 284 18 585 242
Fragments arranged by size:
1352 585 451 284 242 164 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1252 3096
Size: 19 1233 1844
Fragments arranged by size:
1844 1233 19
EcoICRI GAG'CTC
Cuts at: 0 1176 1875 2190 2723 3096
Size: 1176 699 315 533 373
Fragments arranged by size:
1176 699 533 373 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 847 1108 1492 1606 1816 1843 1996
Size: 746 101 261 384 114 210 27 153
Cuts at: 1996 2065 2116 2197 2218 2275 2356 2368 2644
Size: 69 51 81 21 57 81 12 276
Cuts at: 2644 2656 3096
Size: 12 440
Fragments arranged by size:
746 440 384 276 261 210 153 114
101 81 81 69 57 51 27 21
12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 58 3096
Size: 58 3038
EcoO109I rG'GnC_Cy
Cuts at: 0 1467 2334 2619 3096
Size: 1467 867 285 477
Fragments arranged by size:
1467 867 477 285
EcoRV GAT'ATC
Cuts at: 0 3006 3096
Size: 3006 90
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 238 2508 3096
Size: 238 2270 588
Fragments arranged by size:
2270 588 238
FspI TGC'GCA
Cuts at: 0 1222 3096
Size: 1222 1874
FspAI rTGC'GCAy
Cuts at: 0 1222 3096
Size: 1222 1874
HaeII r_GCGC'y
Cuts at: 0 1143 2657 2673 3096
Size: 1143 1514 16 423
Fragments arranged by size:
1514 1143 423 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 928 960 1192 1224 1251 1283 1486 1518
Size: 928 32 232 32 27 32 203 32
Cuts at: 1518 1540 1572 1653 1685 1754 1786 1864 1896
Size: 22 32 81 32 69 32 78 32
Cuts at: 1896 2097 2129 3096
Size: 201 32 967
Fragments arranged by size:
967 928 232 203 201 81 78 69
32 32 32 32 32 32 32 32
32 27 22
HincII GTy'rAC
Cuts at: 0 1010 3096
Size: 1010 2086
HindIII A'AGCT_T
Cuts at: 0 2230 3061 3096
Size: 2230 831 35
Fragments arranged by size:
2230 831 35
KasI G'GCGC_C
Cuts at: 0 2653 3096
Size: 2653 443
KpnI G_GTAC'C
Cuts at: 0 2679 3096
Size: 2679 417
MscI TGG'CCA
Cuts at: 0 1570 2005 2774 3096
Size: 1570 435 769 322
Fragments arranged by size:
1570 769 435 322
MslI CAynn'nnrTG
Cuts at: 0 31 194 589 952 1216 3096
Size: 31 163 395 363 264 1880
Fragments arranged by size:
1880 395 363 264 163 31
MspA1I CmG'CkG
Cuts at: 0 312 966 1401 1602 1821 2139 2538 3096
Size: 312 654 435 201 219 318 399 558
Fragments arranged by size:
654 558 435 399 318 312 219 201
NaeI GCC'GGC
Cuts at: 0 1816 3096
Size: 1816 1280
NarI GG'CG_CC
Cuts at: 0 2654 3096
Size: 2654 442
NcoI C'CATG_G
Cuts at: 0 284 365 3096
Size: 284 81 2731
Fragments arranged by size:
2731 284 81
NdeI CA'TA_TG
Cuts at: 0 138 3096
Size: 138 2958
NgoMIV G'CCGG_C
Cuts at: 0 1814 3096
Size: 1814 1282
NsiI A_TGCA'T
Cuts at: 0 195 3096
Size: 195 2901
NspI r_CATG'y
Cuts at: 0 197 1941 3096
Size: 197 1744 1155
Fragments arranged by size:
1744 1155 197
PasI CC'CwG_GG
Cuts at: 0 1164 3096
Size: 1164 1932
PflMI CCAn_nnn'nTGG
Cuts at: 0 1512 1542 3096
Size: 1512 30 1554
Fragments arranged by size:
1554 1512 30
PfoI T'CCnGG_A
Cuts at: 0 863 1469 2150 3096
Size: 863 606 681 946
Fragments arranged by size:
946 863 681 606
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 976 1008 1732 1764 3096
Size: 976 32 724 32 1332
Fragments arranged by size:
1332 976 724 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1467 2334 3096
Size: 1467 867 762
Fragments arranged by size:
1467 867 762
PshAI GACnn'nnGTC
Cuts at: 0 2422 3096
Size: 2422 674
PsiI TTA'TAA
Cuts at: 0 926 3096
Size: 926 2170
PspXI vC'TCGA_Gb
Cuts at: 0 2420 3096
Size: 2420 676
PstI C_TGCA'G
Cuts at: 0 1466 2105 2291 2879 3096
Size: 1466 639 186 588 217
Fragments arranged by size:
1466 639 588 217 186
PvuII CAG'CTG
Cuts at: 0 312 1821 2139 2538 3096
Size: 312 1509 318 399 558
Fragments arranged by size:
1509 558 399 318 312
SacI G_AGCT'C
Cuts at: 0 1178 1877 2192 2725 3096
Size: 1178 699 315 533 371
Fragments arranged by size:
1178 699 533 371 315
SapI GCTCTTCn'nnn_
Cuts at: 0 1352 1516 2251 2269 3096
Size: 1352 164 735 18 827
Fragments arranged by size:
1352 827 735 164 18
SbfI CC_TGCA'GG
Cuts at: 0 2879 3096
Size: 2879 217
SfcI C'TryA_G
Cuts at: 0 1462 1606 2101 2287 2875 3096
Size: 1462 144 495 186 588 221
Fragments arranged by size:
1462 588 495 221 186 144
SfoI GGC'GCC
Cuts at: 0 2655 3096
Size: 2655 441
SmaI CCC'GGG
Cuts at: 0 2365 3096
Size: 2365 731
SmlI C'TyrA_G
Cuts at: 0 173 379 1333 1989 2338 2420 2497 3096
Size: 173 206 954 656 349 82 77 599
Fragments arranged by size:
954 656 599 349 206 173 82 77
StyI C'CwwG_G
Cuts at: 0 102 284 365 2645 2957 3096
Size: 102 182 81 2280 312 139
Fragments arranged by size:
2280 312 182 139 102 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2808 2901 3096
Size: 2808 93 195
Fragments arranged by size:
2808 195 93
TatI w'GTAC_w
Cuts at: 0 505 2906 3096
Size: 505 2401 190
Fragments arranged by size:
2401 505 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 346 378 1109 1141 3096
Size: 346 32 731 32 1955
Fragments arranged by size:
1955 731 346 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 115 942 3096
Size: 115 827 2154
Fragments arranged by size:
2154 827 115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1581 3096
Size: 1581 1515
XhoI C'TCGA_G
Cuts at: 0 2420 3096
Size: 2420 676
XmaI C'CCGG_G
Cuts at: 0 2363 3096
Size: 2363 733
ZraI GAC'GTC
Cuts at: 0 2412 3096
Size: 2412 684
Enzymes that do cut:
AarI AatII Acc65I AcuI AhdI AlfI AlwNI ApaLI
ApoI AvaI BaeI BanI BanII BbeI BbsI BbvCI
BcgI BfrBI BglII BlpI Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI FspI FspAI HaeII Hin4I HincII HindIII KasI
KpnI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PflMI PfoI PpiI PpuMI
PshAI PsiI PspXI PstI PvuII SacI SapI SbfI
SfcI SfoI SmaI SmlI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AleI AloI
ApaI AscI AseI AsiSI AvrII BamHI BciVI BclI
BglI BmgBI BmrI BmtI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BsrGI BssHII BstBI BstEII BstXI BstZ17I
ClaI CspCI DraI DraIII EagI EcoRI FseI HpaI
MfeI MluI MmeI NheI NotI NruI PacI PciI
PmeI PmlI PspOMI PsrI PvuI RsrII SacII SalI
SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XmnI