(Circular) (Six-base) MAPSORT of: pFN21ASDB0064.seq Check: 6055 from: 1 to: 7225 pFN21ASDB0064 7225 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5035 278 Size: 53 83 186 936 3499 2468 Fragments arranged by size: 3499 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4568 1937 Size: 2631 4594 Acc65I G'GTAC_C Cuts at: 4546 4546 Size: 7225 AclI AA'CG_TT Cuts at: 1563 4044 5352 5725 1563 Size: 2481 1308 373 3063 Fragments arranged by size: 3063 2481 1308 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2979 4389 5288 6672 6940 1632 Size: 1347 1410 899 1384 268 1917 Fragments arranged by size: 1917 1410 1384 1347 899 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7028 AflIII A'CryG_T Cuts at: 1203 2998 3901 6124 7101 1203 Size: 1795 903 2223 977 1327 Fragments arranged by size: 2223 1795 1327 977 903 AgeI A'CCGG_T Cuts at: 6056 6056 Size: 7225 AhdI GACnn_n'nnGTC Cuts at: 5954 5954 Size: 7225 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4157 1184 Size: 2973 4252 AlwNI CAG_nnn'CTG Cuts at: 4210 4968 6540 4210 Size: 758 1572 4895 Fragments arranged by size: 4895 1572 758 ApaI G_GGCC'C Cuts at: 3329 3329 Size: 7225 ApaLI G'TGCA_C Cuts at: 5282 6438 5282 Size: 1156 6069 ApoI r'AATT_y Cuts at: 1493 2118 2381 3883 3965 3985 4534 4760 4912 Size: 625 263 1502 82 20 549 226 152 Cuts at: 4912 7021 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1502 625 549 263 226 152 82 20 AseI AT'TA_AT Cuts at: 160 1032 2724 5779 160 Size: 872 1692 3055 1606 Fragments arranged by size: 3055 1692 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 AvaI C'yCGr_G Cuts at: 1870 1943 2303 2515 3322 4550 1870 Size: 73 360 212 807 1228 4545 Fragments arranged by size: 4545 1228 807 360 212 73 BamHI G'GATC_C Cuts at: 2825 4555 4922 2825 Size: 1730 367 5128 Fragments arranged by size: 5128 1730 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2221 4546 6001 618 Size: 325 217 627 434 2325 1455 1842 Fragments arranged by size: 2325 1842 1455 627 434 325 217 BanII G_rGCy'C Cuts at: 1391 2185 3329 3703 4544 1391 Size: 794 1144 374 841 4072 Fragments arranged by size: 4072 1144 841 794 374 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2150 2678 928 Size: 971 251 528 5475 Fragments arranged by size: 5475 971 528 251 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3763 3797 5416 5450 1903 Size: 34 1826 34 1619 34 3678 Fragments arranged by size: 3678 1826 1619 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5117 6327 5117 Size: 1210 6015 BclI T'GATC_A Cuts at: 1548 1548 Size: 7225 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3090 3127 5836 Size: 107 122 71 631 605 1418 37 2709 Cuts at: 5836 136 Size: 1525 Fragments arranged by size: 2709 1525 1418 631 605 122 107 71 37 BglII A'GATC_T Cuts at: 7220 7220 Size: 7225 BlpI GC'TnA_GC Cuts at: 3186 4642 3186 Size: 1456 5769 Bme1580I G_kGCm'C Cuts at: 1790 2224 3329 5286 6442 1790 Size: 434 1105 1957 1156 2573 Fragments arranged by size: 2573 1957 1156 1105 434 BmgBI CAC'GTC Cuts at: 1318 3904 1318 Size: 2586 4639 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4956 5914 442 Size: 416 53 83 399 24 3539 958 1753 Fragments arranged by size: 3539 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7225 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2565 2589 3063 3435 3483 4226 5885 1239 Size: 1326 24 474 372 48 743 1659 2579 Fragments arranged by size: 2579 1659 1326 743 474 372 48 24 Bpu10I CC'TnA_GC Cuts at: 2271 3532 3702 2271 Size: 1261 170 5794 Fragments arranged by size: 5794 1261 170 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3598 5350 6215 6513 3598 Size: 1752 865 298 4310 Fragments arranged by size: 4310 1752 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5888 882 Size: 617 4389 2219 Fragments arranged by size: 4389 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4522 5016 493 Size: 711 3318 494 2702 Fragments arranged by size: 3318 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7219 7219 Size: 7225 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5032 5414 6049 275 Size: 53 83 186 936 3499 382 635 1451 Fragments arranged by size: 3499 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3007 3794 5658 6056 6330 6477 6888 3007 Size: 787 1864 398 274 147 411 3344 Fragments arranged by size: 3344 1864 787 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2563 2593 3253 3283 3394 Size: 30 165 30 1160 30 660 30 111 Cuts at: 3394 3424 3789 3819 1178 Size: 30 365 30 4584 Fragments arranged by size: 4584 1160 660 365 165 111 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 2619 3123 3408 3507 4242 1186 Size: 961 472 504 285 99 735 4169 Fragments arranged by size: 4169 961 735 504 472 285 99 BseYI C'CCAG_C Cuts at: 3061 3073 3680 3856 6428 6924 3061 Size: 12 607 176 2572 496 3362 Fragments arranged by size: 3362 2572 607 496 176 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4223 4421 1163 Size: 573 2487 198 3967 Fragments arranged by size: 3967 2487 573 198 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4675 5436 6464 1540 Size: 275 186 2674 761 1028 2301 Fragments arranged by size: 2674 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3703 4254 5286 5371 6442 3703 Size: 551 1032 85 1071 4486 Fragments arranged by size: 4486 1071 1032 551 85 BsmI GAATG_Cn' Cuts at: 2434 4080 4276 4736 4829 2434 Size: 1646 196 460 93 4830 Fragments arranged by size: 4830 1646 460 196 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2185 2224 3329 3703 4254 4544 5286 Size: 399 395 39 1105 374 551 290 742 Cuts at: 5286 5371 6442 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1105 1071 742 551 399 395 374 290 85 39 BspEI T'CCGG_A Cuts at: 3007 6888 3007 Size: 3881 3344 BspHI T'CATG_A Cuts at: 5114 6844 5114 Size: 1730 5495 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3882 4149 4580 844 Size: 3038 267 431 3489 Fragments arranged by size: 3489 3038 431 267 BsrBI CCG'CTC Cuts at: 1394 1823 3154 5112 1394 Size: 429 1331 1958 3507 Fragments arranged by size: 3507 1958 1331 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2221 2402 3145 4179 5720 5894 65 Size: 1183 973 181 743 1034 1541 174 1396 Fragments arranged by size: 1541 1396 1183 1034 973 743 181 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5869 6056 1700 Size: 110 4059 187 2869 Fragments arranged by size: 4059 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7225 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7225 BssSI C'ACGA_G Cuts at: 5285 6297 5285 Size: 1012 6213 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7225 BstBI TT'CG_AA Cuts at: 2007 6029 2007 Size: 4022 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7225 BstXI CCAn_nnnn'nTGG Cuts at: 3382 4266 3382 Size: 884 6341 BstYI r'GATC_y Cuts at: 1972 2825 4555 4922 5307 5324 6776 6862 6874 Size: 853 1730 367 385 17 1452 86 12 Cuts at: 6874 7220 1972 Size: 346 1977 Fragments arranged by size: 1977 1730 1452 853 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3693 513 Size: 552 2628 4045 Fragments arranged by size: 4045 2628 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2455 4132 487 Size: 683 56 1229 1677 3580 Fragments arranged by size: 3580 1677 1229 683 56 BtsI GCAGTG_nn' Cuts at: 2783 4113 4254 4741 5535 5555 2783 Size: 1330 141 487 794 20 4453 Fragments arranged by size: 4453 1330 794 487 141 20 ClaI AT'CG_AT Cuts at: 1554 4465 1554 Size: 2911 4314 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7190 DraI TTT'AAA Cuts at: 4529 4529 Size: 7225 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4229 6232 809 Size: 507 2913 2003 1802 Fragments arranged by size: 2913 2003 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3437 4672 5561 Size: 54 1425 50 275 18 1607 1235 889 Cuts at: 5561 7190 8 Size: 1629 43 Fragments arranged by size: 1629 1607 1425 1235 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4672 1537 Size: 275 2860 4090 Fragments arranged by size: 4090 2860 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5155 1353 Size: 3802 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5812 6186 6332 387 Size: 1064 138 4223 374 146 1280 Fragments arranged by size: 4223 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3701 3701 Size: 7225 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2565 2589 2979 3063 3435 3483 4226 Size: 393 933 24 390 84 372 48 743 Cuts at: 4226 4389 5288 5885 6672 6940 1239 Size: 163 899 597 787 268 1524 Fragments arranged by size: 1524 933 899 787 743 597 393 390 372 268 163 84 48 24 EcoO109I rG'GnC_Cy Cuts at: 3083 3101 3110 3119 3326 3677 3083 Size: 18 9 9 207 351 6631 Fragments arranged by size: 6631 351 207 18 9 9 EcoRI G'AATT_C Cuts at: 2118 3965 4534 4912 2118 Size: 1847 569 378 4431 Fragments arranged by size: 4431 1847 569 378 EcoRV GAT'ATC Cuts at: 4309 4309 Size: 7225 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2438 3570 4401 2438 Size: 1132 831 5262 Fragments arranged by size: 5262 1132 831 FspI TGC'GCA Cuts at: 4145 5731 4145 Size: 1586 5639 HaeII r_GCGC'y Cuts at: 1678 6372 1678 Size: 4694 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2485 2517 4458 4490 5866 Size: 32 135 32 913 32 1941 32 1376 Cuts at: 5866 5898 5940 5972 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1941 1376 913 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2193 2751 3015 3567 4048 4569 Size: 988 281 255 558 264 552 481 521 Cuts at: 4569 4815 669 Size: 246 3079 Fragments arranged by size: 3079 988 558 552 521 481 281 264 255 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7225 HpaI GTT'AAC Cuts at: 2193 2751 4815 2193 Size: 558 2064 4603 Fragments arranged by size: 4603 2064 558 KpnI G_GTAC'C Cuts at: 4550 4550 Size: 7225 MfeI C'AATT_G Cuts at: 2838 4824 2838 Size: 1986 5239 MluI A'CGCG_T Cuts at: 7101 7101 Size: 7225 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3448 4432 6339 6523 1305 Size: 2143 984 1907 184 2007 Fragments arranged by size: 2143 2007 1907 984 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5403 Fragments arranged by size: 5403 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5183 5542 5701 518 Size: 648 4017 359 159 2042 Fragments arranged by size: 4017 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3065 3860 4641 5318 6466 6711 1606 Size: 1459 795 781 677 1148 245 2120 Fragments arranged by size: 2120 1459 1148 795 781 677 245 NcoI C'CATG_G Cuts at: 513 1065 3693 513 Size: 552 2628 4045 Fragments arranged by size: 4045 2628 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7225 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7225 NotI GC'GGCC_GC Cuts at: 4672 4672 Size: 7225 NspI r_CATG'y Cuts at: 1132 1229 3002 3982 4054 4583 6128 1132 Size: 97 1773 980 72 529 1545 2229 Fragments arranged by size: 2229 1773 1545 980 529 97 72 PasI CC'CwG_GG Cuts at: 1348 3116 4075 1348 Size: 1768 959 4498 Fragments arranged by size: 4498 1768 959 PciI A'CATG_T Cuts at: 2998 6124 2998 Size: 3126 4099 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7225 PfoI T'CCnGG_A Cuts at: 3451 3451 Size: 7225 PmeI GTTT'AAAC Cuts at: 4529 4529 Size: 7225 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5294 5326 6807 6839 5294 Size: 32 1481 32 5680 Fragments arranged by size: 5680 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3101 3677 3101 Size: 576 6649 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7225 PsiI TTA'TAA Cuts at: 2591 4041 4795 2591 Size: 1450 754 5021 Fragments arranged by size: 5021 1450 754 PspOMI G'GGCC_C Cuts at: 3325 3325 Size: 7225 PstI C_TGCA'G Cuts at: 830 4498 4577 830 Size: 3668 79 3478 Fragments arranged by size: 3668 3478 79 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7225 PvuII CAG'CTG Cuts at: 3065 3860 3065 Size: 795 6430 SacI G_AGCT'C Cuts at: 3703 3703 Size: 7225 SalI G'TCGA_C Cuts at: 1936 4567 1936 Size: 2631 4594 SanDI GG'GwC_CC Cuts at: 3677 3677 Size: 7225 SbfI CC_TGCA'GG Cuts at: 4577 4577 Size: 7225 ScaI AGT'ACT Cuts at: 5473 7185 5473 Size: 1712 5513 SfcI C'TryA_G Cuts at: 826 1045 2094 2290 2439 3289 4494 4573 5708 Size: 219 1049 196 149 850 1205 79 1135 Cuts at: 5708 6389 6580 826 Size: 681 191 1471 Fragments arranged by size: 1471 1205 1135 1049 850 681 219 196 191 149 79 SfiI GGCCn_nnn'nGGCC Cuts at: 3090 3127 3090 Size: 37 7188 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7225 SmaI CCC'GGG Cuts at: 1872 2517 4552 1872 Size: 645 2035 4545 Fragments arranged by size: 4545 2035 645 SmlI C'TyrA_G Cuts at: 820 1017 1943 3577 5329 6230 6492 820 Size: 197 926 1634 1752 901 262 1553 Fragments arranged by size: 1752 1634 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4522 5016 493 Size: 4029 494 2702 Fragments arranged by size: 4029 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7225 SphI G_CATG'C Cuts at: 1132 4583 1132 Size: 3451 3774 SspI AAT'ATT Cuts at: 5 52 4290 5149 5 Size: 47 4238 859 2081 Fragments arranged by size: 4238 2081 859 47 StuI AGG'CCT Cuts at: 1517 2057 2758 1517 Size: 540 701 5984 Fragments arranged by size: 5984 701 540 StyI C'CwwG_G Cuts at: 513 1065 3020 3693 3960 4664 513 Size: 552 1955 673 267 704 3074 Fragments arranged by size: 3074 1955 704 673 552 267 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3298 5252 5269 5422 5607 6052 325 Size: 1255 1718 1954 17 153 185 445 1498 Fragments arranged by size: 1954 1718 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3142 5471 7183 Size: 274 80 33 51 1443 1165 2329 1712 Cuts at: 7183 96 Size: 138 Fragments arranged by size: 2329 1712 1443 1165 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2563 2595 3331 3363 3394 3426 3789 3821 7185 Size: 32 736 32 31 32 363 32 3364 Cuts at: 7185 7217 2563 Size: 32 2571 Fragments arranged by size: 3364 2571 736 363 32 32 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7225 XbaI T'CTAG_A Cuts at: 4561 4561 Size: 7225 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2548 2902 4263 2548 Size: 354 1361 5510 Fragments arranged by size: 5510 1361 354 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7225 XmaI C'CCGG_G Cuts at: 1870 2515 4550 1870 Size: 645 2035 4545 Fragments arranged by size: 4545 2035 645 XmnI GAAnn'nnTTC Cuts at: 5354 5354 Size: 7225 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5033 276 Size: 53 83 186 936 3499 2468 Fragments arranged by size: 3499 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI