Restriction map for
pFN21ASDB0061
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# NO CUT ENZYMES ( 76 enzymes) #
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Acc65I AccI AclI AflII AgeI
AocI AscI AseI AsnI Asp718I
AspI AvrII BciVI BclI BfaI
BfrI BlnI BsaBI BsiWI BsmI
Bsp1407I BspDI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
Eco81I EcoT22I FbaI FseI HpaI
KpnI MaeI MamI MfeI MluI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 1534 GACGTC GACGT^C
AhdI 3120 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1728 GTGCAC G^TGCAC
ApaLI 1728 GTGCAC G^TGCAC
Asp700I 114 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3120 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 791 GGATCC G^GATCC
BbrPI 2119 CACGTG CAC^GTG
BbvCI 2469 CCTCAGC CCTCAGC(-5/-2)
BsgI 1279 GTGCAG GTGCAG(16/14)
BspLU11I 275 ACATGT A^CATGT
BstAPI 1440 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2301 GGTCACC G^GT[ACGT]ACC
BstPI 2301 GGTCACC G^GT[ACGT]ACC
Eam1105I 3120 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2301 GGTCACC G^GT[ACGT]ACC
EcoRI 3016 GAATTC G^AATTC
EcoRV 424 GATATC GAT^ATC
HincII 1978 GTCAAC GT[CT]^[GA]AC
HindII 1978 GTCAAC GT[CT]^[GA]AC
HindIII 2156 AAGCTT A^AGCTT
PaeR7I 451 CTCGAG C^TCGAG
PmaCI 2119 CACGTG CAC^GTG
PmlI 2119 CACGTG CAC^GTG
SacI 2763 GAGCTC GAGCT^C
SapI 1626 GCTCTTC GCTCTTC(1/4)
SfiI 3253 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3050 GCATGC GCATG^C
XhoI 451 CTCGAG C^TCGAG
XmnI 114 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 1885 AAATTC [GA]^AATT[CT]
3016 GAATTC
AflIII 275 ACATGT A^C[GA][CT]GT
1388 ACGTGT
ApoI 1885 AAATTC [GA]^AATT[CT]
3016 GAATTC
AviII 479 TGCGCA TGC^GCA
1847 TGCGCA
BbeI 1661 GGCGCC GGCGC^C
2114 GGCGCC
BglII 557 AGATCT A^GATCT
1172 AGATCT
BlpI 1353 GCTGAGC GC^T[ACGT]AGC
3177 GCTGAGC
Bpu1102I 1353 GCTGAGC GC^T[ACGT]AGC
3177 GCTGAGC
BsaI 1765 GAGACC GGTCTC(1/5)
1524 GGTCTC
BsmBI 1562 GAGACG CGTCTC(1/5)
1918 GAGACG
BspHI 680 TCATGA T^CATGA
1370 TCATGA
BsrDI 96 CATTGC GCAATG(2/0)
2864 GCAATG
CelII 1353 GCTGAGC GC^T[ACGT]AGC
3177 GCTGAGC
DraIII 34 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
1807 CACCAGGTG
FspI 479 TGCGCA TGC^GCA
1847 TGCGCA
KasI 1661 GGCGCC G^GCGCC
2114 GGCGCC
KspI 1927 CCGCGG CCGC^GG
2685 CCGCGG
NarI 1661 GGCGCC GG^CGCC
2114 GGCGCC
PpuMI 1462 GGGACCC [GA]G^G[AT]CC[CT]
2397 GGGACCT
RcaI 680 TCATGA T^CATGA
1370 TCATGA
SacII 1927 CCGCGG CCGC^GG
2685 CCGCGG
ScaI 509 AGTACT AGT^ACT
1310 AGTACT
SfoI 1661 GGCGCC GGC^GCC
2114 GGCGCC
SmlI 451 CTCGAG C^T[CT][GA]AG
2710 CTCAAG
TfiI 243 GAATC G^A[AT]TC
2296 GATTC
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# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BcnI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmaI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI