(Circular) (Six-base) MAPSORT of: pFN21ASDB0061.seq Check: 6156 from: 1 to: 8023 pFN21ASDB0061 8023 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3996 3996 Size: 8023 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3561 5833 278 Size: 53 83 186 936 2025 2272 2468 Fragments arranged by size: 2468 2272 2025 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5366 1937 Size: 3429 4594 Acc65I G'GTAC_C Cuts at: 5344 5344 Size: 8023 AclI AA'CG_TT Cuts at: 1563 6150 6523 1563 Size: 4587 373 3063 Fragments arranged by size: 4587 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3299 3872 4618 6086 7470 7738 1632 Size: 1667 573 746 1468 1384 268 1917 Fragments arranged by size: 1917 1667 1468 1384 746 573 268 AfeI AGC'GCT Cuts at: 2039 2141 4595 2039 Size: 102 2454 5467 Fragments arranged by size: 5467 2454 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7826 AflIII A'CryG_T Cuts at: 1203 2298 3411 6922 7899 1203 Size: 1095 1113 3511 977 1327 Fragments arranged by size: 3511 1327 1113 1095 977 AgeI A'CCGG_T Cuts at: 6854 6854 Size: 8023 AhdI GACnn_n'nnGTC Cuts at: 5148 6752 5148 Size: 1604 6419 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8023 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3188 3939 4910 1184 Size: 2004 751 971 4297 Fragments arranged by size: 4297 2004 971 751 AlwNI CAG_nnn'CTG Cuts at: 2662 2695 2836 2886 3009 3457 3469 3988 4288 Size: 33 141 50 123 448 12 519 300 Cuts at: 4288 4477 5111 5766 7338 2662 Size: 189 634 655 1572 3347 Fragments arranged by size: 3347 1572 655 634 519 448 300 189 141 123 50 33 12 ApaI G_GGCC'C Cuts at: 4919 4973 5167 4919 Size: 54 194 7775 Fragments arranged by size: 7775 194 54 ApaLI G'TGCA_C Cuts at: 3751 6080 7236 3751 Size: 2329 1156 4538 Fragments arranged by size: 4538 2329 1156 ApoI r'AATT_y Cuts at: 1493 3908 5039 5332 5558 5710 7819 1493 Size: 2415 1131 293 226 152 2109 1697 Fragments arranged by size: 2415 2109 1697 1131 293 226 152 AseI AT'TA_AT Cuts at: 160 1032 6577 160 Size: 872 5545 1606 Fragments arranged by size: 5545 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8023 AvaI C'yCGr_G Cuts at: 1870 1943 2474 2907 3656 3801 3938 4271 4851 Size: 73 531 433 749 145 137 333 580 Cuts at: 4851 4965 5007 5248 5348 1870 Size: 114 42 241 100 4545 Fragments arranged by size: 4545 749 580 531 433 333 241 145 137 114 100 73 42 BamHI G'GATC_C Cuts at: 2814 5353 5720 2814 Size: 2539 367 5117 Fragments arranged by size: 5117 2539 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3684 4137 4685 4894 4949 Size: 325 217 627 1897 453 548 209 55 Cuts at: 4949 5157 5344 6799 618 Size: 208 187 1455 1842 Fragments arranged by size: 1897 1842 1455 627 548 453 325 217 209 208 187 55 BanII G_rGCy'C Cuts at: 1391 3093 3383 4014 4790 4919 4973 5167 5342 Size: 1702 290 631 776 129 54 194 175 Cuts at: 5342 1391 Size: 4072 Fragments arranged by size: 4072 1702 776 631 290 194 175 129 54 BbeI G_GCGC'C Cuts at: 3688 4141 3688 Size: 453 7570 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2118 2375 2898 4552 928 Size: 971 219 257 523 1654 4399 Fragments arranged by size: 4399 1654 971 523 257 219 BbvCI CC'TCA_GC Cuts at: 4493 4493 Size: 8023 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3217 3251 3974 4008 4091 4125 4579 Size: 34 1280 34 723 34 83 34 454 Cuts at: 4579 4613 6214 6248 1903 Size: 34 1601 34 3678 Fragments arranged by size: 3678 1601 1280 723 454 83 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5915 7125 5915 Size: 1210 6813 BclI T'GATC_A Cuts at: 1548 1548 Size: 8023 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2526 3012 3387 Size: 107 122 71 631 605 854 486 375 Cuts at: 3387 3955 5283 6634 136 Size: 568 1328 1351 1525 Fragments arranged by size: 1525 1351 1328 854 631 605 568 486 375 122 107 71 BglII A'GATC_T Cuts at: 2580 3195 5313 8018 2580 Size: 615 2118 2705 2585 Fragments arranged by size: 2705 2585 2118 615 BlpI GC'TnA_GC Cuts at: 3377 5201 5440 3377 Size: 1824 239 5960 Fragments arranged by size: 5960 1824 239 Bme1580I G_kGCm'C Cuts at: 1790 3755 4899 4919 4952 4973 5014 5167 6084 Size: 1965 1144 20 33 21 41 153 917 Cuts at: 6084 7240 1790 Size: 1156 2573 Fragments arranged by size: 2573 1965 1156 1144 917 153 41 33 21 20 BmgBI CAC'GTC Cuts at: 1318 2440 3412 3945 5015 1318 Size: 1122 972 533 1070 4326 Fragments arranged by size: 4326 1122 1070 972 533 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2251 3817 5754 Size: 416 53 83 399 24 834 1566 1937 Cuts at: 5754 6712 442 Size: 958 1753 Fragments arranged by size: 1937 1753 1566 958 834 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8023 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3608 4508 3608 Size: 900 7123 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2618 3529 4097 4280 4571 4949 4962 6683 Size: 1379 911 568 183 291 378 13 1721 Cuts at: 6683 1239 Size: 2579 Fragments arranged by size: 2579 1721 1379 911 568 378 291 183 13 Bpu10I CC'TnA_GC Cuts at: 4493 4493 Size: 8023 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4718 6148 7013 7311 4718 Size: 1430 865 298 5430 Fragments arranged by size: 5430 1430 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3553 3782 6686 882 Size: 617 2054 229 2904 2219 Fragments arranged by size: 2904 2219 2054 617 229 BsaAI yAC'GTr Cuts at: 493 1204 4144 5179 5305 5320 5814 493 Size: 711 2940 1035 126 15 494 2702 Fragments arranged by size: 2940 2702 1035 711 494 126 15 BsaBI GATnn'nnATC Cuts at: 8017 8017 Size: 8023 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 3558 3685 3978 Size: 53 83 186 936 582 1443 127 293 Cuts at: 3978 4138 4376 5830 6212 6847 275 Size: 160 238 1454 382 635 1451 Fragments arranged by size: 1454 1451 1443 936 635 582 382 293 238 186 160 127 83 53 BsaWI w'CCGG_w Cuts at: 2133 2304 2710 3642 4152 6456 6854 7128 7275 Size: 171 406 932 510 2304 398 274 147 Cuts at: 7275 7686 2133 Size: 411 2470 Fragments arranged by size: 2470 2304 932 510 411 406 398 274 171 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4922 4952 1178 Size: 30 165 30 3519 30 4249 Fragments arranged by size: 4249 3519 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3308 3827 4094 4178 4277 4616 1186 Size: 2122 519 267 84 99 339 4593 Fragments arranged by size: 4593 2122 519 339 267 99 84 BseYI C'CCAG_C Cuts at: 2363 2822 2950 3009 3689 4671 5133 7226 7722 Size: 459 128 59 680 982 462 2093 496 Cuts at: 7722 2363 Size: 2664 Fragments arranged by size: 2664 2093 982 680 496 462 459 128 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3323 1163 Size: 573 1587 5863 Fragments arranged by size: 5863 1587 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2227 4339 4747 5473 6234 7262 Size: 275 186 226 2112 408 726 761 1028 Cuts at: 7262 1540 Size: 2301 Fragments arranged by size: 2301 2112 1028 761 726 408 275 226 186 BsiHKAI G_wGCw'C Cuts at: 2184 3755 4037 4734 4790 5031 6084 6169 7240 Size: 1571 282 697 56 241 1053 85 1071 Cuts at: 7240 2184 Size: 2967 Fragments arranged by size: 2967 1571 1071 1053 697 282 241 85 56 BsmI GAATG_Cn' Cuts at: 5534 5627 5534 Size: 93 7930 BsmBI CGTCTCn'nnnn_ Cuts at: 3579 3935 3579 Size: 356 7667 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 3093 3383 3755 4014 4037 4734 Size: 399 394 909 290 372 259 23 697 Cuts at: 4734 4790 4899 4919 4952 4973 5014 5031 5167 Size: 56 109 20 33 21 41 17 136 Cuts at: 5167 5342 6084 6169 7240 1391 Size: 175 742 85 1071 2174 Fragments arranged by size: 2174 1071 909 742 697 399 394 372 290 259 175 136 109 85 56 41 33 23 21 20 17 BspEI T'CCGG_A Cuts at: 2133 3642 4152 7686 2133 Size: 1509 510 3534 2470 Fragments arranged by size: 3534 2470 1509 510 BspHI T'CATG_A Cuts at: 2703 3393 5912 7642 2703 Size: 690 2519 1730 3084 Fragments arranged by size: 3084 2519 1730 690 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2794 2955 3996 5378 844 Size: 1950 161 1041 1382 3489 Fragments arranged by size: 3489 1950 1382 1041 161 BsrBI CCG'CTC Cuts at: 1394 1823 2073 4210 4267 4303 5910 1394 Size: 429 250 2137 57 36 1607 3507 Fragments arranged by size: 3507 2137 1607 429 250 57 36 BsrDI GCAATG_nn' Cuts at: 65 1248 2118 4894 6518 6692 65 Size: 1183 870 2776 1624 174 1396 Fragments arranged by size: 2776 1624 1396 1183 870 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3515 3698 3878 5069 5265 6667 6854 Size: 110 1705 183 180 1191 196 1402 187 Cuts at: 6854 1700 Size: 2869 Fragments arranged by size: 2869 1705 1402 1191 196 187 183 180 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8023 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8023 BssSI C'ACGA_G Cuts at: 2849 3116 3491 6083 7095 2849 Size: 267 375 2592 1012 3777 Fragments arranged by size: 3777 2592 1012 375 267 BstAPI GCAn_nnn'nTGC Cuts at: 794 3469 794 Size: 2675 5348 BstBI TT'CG_AA Cuts at: 2007 6827 2007 Size: 4820 3203 BstEII G'GTnAC_C Cuts at: 1184 4324 1184 Size: 3140 4883 BstXI CCAn_nnnn'nTGG Cuts at: 3209 3539 3730 4879 4987 3209 Size: 330 191 1149 108 6245 Fragments arranged by size: 6245 1149 330 191 108 BstYI r'GATC_y Cuts at: 1972 2541 2580 2814 2859 3195 5229 5242 5313 Size: 569 39 234 45 336 2034 13 71 Cuts at: 5313 5353 5720 6105 6122 7574 7660 7672 8018 Size: 40 367 385 17 1452 86 12 346 Cuts at: 8018 1972 Size: 1977 Fragments arranged by size: 2034 1977 1452 569 385 367 346 336 234 86 71 45 40 39 17 13 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3432 3950 4708 513 Size: 552 957 1410 518 758 3828 Fragments arranged by size: 3828 1410 957 758 552 518 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4785 487 Size: 683 56 3559 3725 Fragments arranged by size: 3725 3559 683 56 BtsI GCAGTG_nn' Cuts at: 3455 4734 5539 6333 6353 3455 Size: 1279 805 794 20 5125 Fragments arranged by size: 5125 1279 805 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8023 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7988 DraI TTT'AAA Cuts at: 5327 5327 Size: 8023 DraIII CAC_nnn'GTG Cuts at: 2062 3835 2062 Size: 1773 6250 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7030 809 Size: 507 5714 1802 Fragments arranged by size: 5714 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3081 3735 3947 Size: 54 1425 50 275 18 1251 654 212 Cuts at: 3947 4812 5067 5102 5275 5470 6359 7988 8 Size: 865 255 35 173 195 889 1629 43 Fragments arranged by size: 1629 1425 1251 889 865 654 275 255 212 195 173 54 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 5470 1537 Size: 275 3658 4090 Fragments arranged by size: 4090 3658 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3110 3150 3599 3656 5953 1353 Size: 1757 40 449 57 2297 3423 Fragments arranged by size: 3423 2297 1757 449 57 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2433 5007 6610 6984 7130 387 Size: 1064 138 844 2574 1603 374 146 1280 Fragments arranged by size: 2574 1603 1280 1064 844 374 146 138 EcoICRI GAG'CTC Cuts at: 4788 4788 Size: 8023 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2618 3299 3529 3872 4097 4280 4571 Size: 393 986 681 230 343 225 183 291 Cuts at: 4571 4618 4949 4962 6086 6683 7470 7738 1239 Size: 47 331 13 1124 597 787 268 1524 Fragments arranged by size: 1524 1124 986 787 681 597 393 343 331 291 268 230 225 183 47 13 EcoNI CCTnn'n_nnAGG Cuts at: 2243 3083 3119 4076 4079 4526 2243 Size: 840 36 957 3 447 5740 Fragments arranged by size: 5740 957 840 447 36 3 EcoO109I rG'GnC_Cy Cuts at: 2670 3486 3798 4421 4617 4915 4916 4969 4970 Size: 816 312 623 196 298 1 53 1 Cuts at: 4970 5164 5284 2670 Size: 194 120 5409 Fragments arranged by size: 5409 816 623 312 298 196 194 120 53 1 1 EcoRI G'AATT_C Cuts at: 5039 5332 5710 5039 Size: 293 378 7352 Fragments arranged by size: 7352 378 293 EcoRV GAT'ATC Cuts at: 2449 2449 Size: 8023 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2056 4381 2056 Size: 2325 5698 FspI TGC'GCA Cuts at: 2504 3872 6529 2504 Size: 1368 2657 3998 Fragments arranged by size: 3998 2657 1368 HaeII r_GCGC'y Cuts at: 1678 2041 2143 3516 3688 4141 4597 7170 1678 Size: 363 102 1373 172 453 456 2573 2531 Fragments arranged by size: 2573 2531 1373 456 453 363 172 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4922 4954 5134 5166 6664 Size: 32 135 32 3350 32 180 32 1498 Cuts at: 6664 6696 6738 6770 1373 Size: 32 42 32 2626 Fragments arranged by size: 3350 2626 1498 180 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4003 5367 5613 669 Size: 988 281 2065 1364 246 3079 Fragments arranged by size: 3079 2065 1364 988 281 246 HindIII A'AGCT_T Cuts at: 748 4179 748 Size: 3431 4592 HpaI GTT'AAC Cuts at: 5613 5613 Size: 8023 KasI G'GCGC_C Cuts at: 3684 4137 3684 Size: 453 7570 KpnI G_GTAC'C Cuts at: 5348 5348 Size: 8023 MfeI C'AATT_G Cuts at: 5622 5622 Size: 8023 MluI A'CGCG_T Cuts at: 7899 7899 Size: 8023 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2487 2610 3624 4209 4727 7137 7321 1305 Size: 1182 123 1014 585 518 2410 184 2007 Fragments arranged by size: 2410 2007 1182 1014 585 518 184 123 MscI TGG'CCA Cuts at: 10 64 1489 1832 3083 3737 4814 10 Size: 54 1425 343 1251 654 1077 3219 Fragments arranged by size: 3219 1425 1251 1077 654 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3035 3170 3743 3866 5981 6340 6499 Size: 648 1869 135 573 123 2115 359 159 Cuts at: 6499 518 Size: 2042 Fragments arranged by size: 2115 2042 1869 648 573 359 159 135 123 MspA1I CmG'CkG Cuts at: 1606 2470 2659 2836 2964 3009 3318 3507 3564 Size: 864 189 177 128 45 309 189 57 Cuts at: 3564 3771 3952 4710 5439 6116 7264 7509 1606 Size: 207 181 758 729 677 1148 245 2120 Fragments arranged by size: 2120 1148 864 758 729 677 309 245 207 189 189 181 177 128 57 45 NaeI GCC'GGC Cuts at: 3517 3700 3880 5071 5267 3517 Size: 183 180 1191 196 6273 Fragments arranged by size: 6273 1191 196 183 180 NarI GG'CG_CC Cuts at: 3685 4138 3685 Size: 453 7570 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6514 Fragments arranged by size: 6514 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8023 NgoMIV G'CCGG_C Cuts at: 3515 3698 3878 5069 5265 3515 Size: 183 180 1191 196 6273 Fragments arranged by size: 6273 1191 196 183 180 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8023 NotI GC'GGCC_GC Cuts at: 5470 5470 Size: 8023 NspI r_CATG'y Cuts at: 1132 1229 2302 2314 5077 5381 6926 1132 Size: 97 1073 12 2763 304 1545 2229 Fragments arranged by size: 2763 2229 1545 1073 304 97 12 PasI CC'CwG_GG Cuts at: 1348 4987 1348 Size: 3639 4384 PciI A'CATG_T Cuts at: 2298 6922 2298 Size: 4624 3399 PflMI CCAn_nnn'nTGG Cuts at: 1108 2662 2836 5065 5111 5179 5191 1108 Size: 1554 174 2229 46 68 12 3940 Fragments arranged by size: 3940 2229 1554 174 68 46 12 PfoI T'CCnGG_A Cuts at: 4074 4548 4074 Size: 474 7549 PmeI GTTT'AAAC Cuts at: 5327 5327 Size: 8023 PmlI CAC'GTG Cuts at: 4144 4144 Size: 8023 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2531 2563 4082 4114 6092 6124 7605 7637 2531 Size: 32 1519 32 1978 32 1481 32 2917 Fragments arranged by size: 2917 1978 1519 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3486 4421 3486 Size: 935 7088 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8023 PsiI TTA'TAA Cuts at: 5593 5593 Size: 8023 PspOMI G'GGCC_C Cuts at: 4915 4969 5163 4915 Size: 54 194 7775 Fragments arranged by size: 7775 194 54 PspXI vC'TCGA_Gb Cuts at: 2474 2474 Size: 8023 PstI C_TGCA'G Cuts at: 830 2460 2847 2976 3255 3465 3474 3918 5375 Size: 1630 387 129 279 210 9 444 1457 Cuts at: 5375 830 Size: 3478 Fragments arranged by size: 3478 1630 1457 444 387 279 210 129 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8023 PvuII CAG'CTG Cuts at: 2659 2836 3507 2659 Size: 177 671 7175 Fragments arranged by size: 7175 671 177 SacI G_AGCT'C Cuts at: 4790 4790 Size: 8023 SacII CC_GC'GG Cuts at: 3953 4711 3953 Size: 758 7265 SalI G'TCGA_C Cuts at: 1936 5365 1936 Size: 3429 4594 SanDI GG'GwC_CC Cuts at: 3486 3486 Size: 8023 SapI GCTCTTCn'nnn_ Cuts at: 3656 3656 Size: 8023 SbfI CC_TGCA'GG Cuts at: 5375 5375 Size: 8023 ScaI AGT'ACT Cuts at: 2534 3335 6271 7983 2534 Size: 801 2936 1712 2574 Fragments arranged by size: 2936 2574 1712 801 SexAI A'CCwGG_T Cuts at: 2748 3729 3831 2748 Size: 981 102 6940 Fragments arranged by size: 6940 981 102 SfcI C'TryA_G Cuts at: 826 1045 2456 2843 2972 3251 3461 3470 3914 Size: 219 1411 387 129 279 210 9 444 Cuts at: 3914 4646 5371 6506 7187 7378 826 Size: 732 725 1135 681 191 1471 Fragments arranged by size: 1471 1411 1135 732 725 681 444 387 279 219 210 191 129 9 SfiI GGCCn_nnn'nGGCC Cuts at: 5283 5283 Size: 8023 SfoI GGC'GCC Cuts at: 3686 4139 3686 Size: 453 7570 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8023 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8023 SmaI CCC'GGG Cuts at: 1872 4853 4967 5009 5350 1872 Size: 2981 114 42 341 4545 Fragments arranged by size: 4545 2981 341 114 42 SmlI C'TyrA_G Cuts at: 820 1017 1943 2474 4733 6127 7028 7290 820 Size: 197 926 531 2259 1394 901 262 1553 Fragments arranged by size: 2259 1553 1394 926 901 531 262 197 SnaBI TAC'GTA Cuts at: 493 5320 5814 493 Size: 4827 494 2702 Fragments arranged by size: 4827 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8023 SphI G_CATG'C Cuts at: 1132 5077 5381 1132 Size: 3945 304 3774 Fragments arranged by size: 3945 3774 304 SspI AAT'ATT Cuts at: 5 52 5947 5 Size: 47 5895 2081 Fragments arranged by size: 5895 2081 47 StuI AGG'CCT Cuts at: 1517 2480 3635 4130 4960 1517 Size: 963 1155 495 830 4580 Fragments arranged by size: 4580 1155 963 830 495 StyI C'CwwG_G Cuts at: 513 1065 2022 2406 3084 4038 4815 5462 513 Size: 552 957 384 678 954 777 647 3074 Fragments arranged by size: 3074 957 954 777 678 647 552 384 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4312 4583 4967 6050 6067 6220 6405 Size: 1255 2732 271 384 1083 17 153 185 Cuts at: 6405 6850 325 Size: 445 1498 Fragments arranged by size: 2732 1498 1255 1083 445 384 271 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 3159 3333 Size: 274 80 33 51 1443 555 627 174 Cuts at: 3333 6269 7981 96 Size: 2936 1712 138 Fragments arranged by size: 2936 1712 1443 627 555 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3398 3430 5289 5321 7983 8015 3398 Size: 32 1859 32 2662 32 3406 Fragments arranged by size: 3406 2662 1859 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8023 XbaI T'CTAG_A Cuts at: 5359 5359 Size: 8023 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2593 2995 3536 3730 2389 Size: 204 402 541 194 6682 Fragments arranged by size: 6682 541 402 204 194 XhoI C'TCGA_G Cuts at: 1943 2474 1943 Size: 531 7492 XmaI C'CCGG_G Cuts at: 1870 4851 4965 5007 5348 1870 Size: 2981 114 42 341 4545 Fragments arranged by size: 4545 2981 341 114 42 XmnI GAAnn'nnTTC Cuts at: 2141 6152 2141 Size: 4011 4012 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3559 5831 276 Size: 53 83 186 936 2025 2272 2468 Fragments arranged by size: 2468 2272 2025 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I FseI FspAI NruI NsiI PacI PsrI RsrII SrfI SwaI