Restriction map for
pFN21ASDB0061
(Linear) (Six-base) MAPSORT of: KIBB0061.seq Check: 3602 from: 1 to: 3294
KIBB0061.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1973 3294
Size: 1973 1321
AatII G_ACGT'C
Cuts at: 0 1538 3294
Size: 1538 1756
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1849 2595 3294
Size: 1276 573 746 699
Fragments arranged by size:
1276 746 699 573
AfeI AGC'GCT
Cuts at: 0 16 118 2572 3294
Size: 16 102 2454 722
Fragments arranged by size:
2454 722 102 16
AflIII A'CryG_T
Cuts at: 0 275 1388 3294
Size: 275 1113 1906
Fragments arranged by size:
1906 1113 275
AhdI GACnn_n'nnGTC
Cuts at: 0 3125 3294
Size: 3125 169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1165 1916 2887 3294
Size: 1165 751 971 407
Fragments arranged by size:
1165 971 751 407
AlwNI CAG_nnn'CTG
Cuts at: 0 639 672 813 863 986 1434 1446 1965
Size: 639 33 141 50 123 448 12 519
Cuts at: 1965 2265 2454 3088 3294
Size: 300 189 634 206
Fragments arranged by size:
639 634 519 448 300 206 189 141
123 50 33 12
ApaI G_GGCC'C
Cuts at: 0 2896 2950 3144 3294
Size: 2896 54 194 150
Fragments arranged by size:
2896 194 150 54
ApaLI G'TGCA_C
Cuts at: 0 1728 3294
Size: 1728 1566
ApoI r'AATT_y
Cuts at: 0 1885 3016 3294
Size: 1885 1131 278
Fragments arranged by size:
1885 1131 278
AvaI C'yCGr_G
Cuts at: 0 451 884 1633 1778 1915 2248 2828 2942
Size: 451 433 749 145 137 333 580 114
Cuts at: 2942 2984 3225 3294
Size: 42 241 69
Fragments arranged by size:
749 580 451 433 333 241 145 137
114 69 42
BamHI G'GATC_C
Cuts at: 0 791 3294
Size: 791 2503
BanI G'GyrC_C
Cuts at: 0 1661 2114 2662 2871 2926 3134 3294
Size: 1661 453 548 209 55 208 160
Fragments arranged by size:
1661 548 453 209 208 160 55
BanII G_rGCy'C
Cuts at: 0 1070 1360 1991 2767 2896 2950 3144 3294
Size: 1070 290 631 776 129 54 194 150
Fragments arranged by size:
1070 776 631 290 194 150 129 54
BbeI G_GCGC'C
Cuts at: 0 1665 2118 3294
Size: 1665 453 1176
Fragments arranged by size:
1665 1176 453
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 352 875 2529 3294
Size: 95 257 523 1654 765
Fragments arranged by size:
1654 765 523 257 95
BbvCI CC'TCA_GC
Cuts at: 0 2470 3294
Size: 2470 824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1194 1228 1951 1985 2068 2102 2556 2590
Size: 1194 34 723 34 83 34 454 34
Cuts at: 2590 3294
Size: 704
Fragments arranged by size:
1194 723 704 454 83 34 34 34
34
BglI GCCn_nnn'nGGC
Cuts at: 0 503 989 1364 1932 3260 3294
Size: 503 486 375 568 1328 34
Fragments arranged by size:
1328 568 503 486 375 34
BglII A'GATC_T
Cuts at: 0 557 1172 3294
Size: 557 615 2122
Fragments arranged by size:
2122 615 557
BlpI GC'TnA_GC
Cuts at: 0 1354 3178 3294
Size: 1354 1824 116
Fragments arranged by size:
1824 1354 116
Bme1580I G_kGCm'C
Cuts at: 0 1732 2876 2896 2929 2950 2991 3144 3294
Size: 1732 1144 20 33 21 41 153 150
Fragments arranged by size:
1732 1144 153 150 41 33 21 20
BmgBI CAC'GTC
Cuts at: 0 417 1389 1922 2992 3294
Size: 417 972 533 1070 302
Fragments arranged by size:
1070 972 533 417 302
BmrI ACTGGGnnnn_n'
Cuts at: 0 228 1794 3294
Size: 228 1566 1500
Fragments arranged by size:
1566 1500 228
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1585 2485 3294
Size: 1585 900 809
Fragments arranged by size:
1585 900 809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1506 2074 2257 2548 2926 2939 3294
Size: 595 911 568 183 291 378 13 355
Fragments arranged by size:
911 595 568 378 355 291 183 13
Bpu10I CC'TnA_GC
Cuts at: 0 2470 3294
Size: 2470 824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2695 3294
Size: 2695 599
BsaI GGTCTCn'nnnn_
Cuts at: 0 1530 1759 3294
Size: 1530 229 1535
Fragments arranged by size:
1535 1530 229
BsaAI yAC'GTr
Cuts at: 0 2121 3156 3282 3294
Size: 2121 1035 126 12
Fragments arranged by size:
2121 1035 126 12
BsaHI Gr'CG_yC
Cuts at: 0 92 1535 1662 1955 2115 2353 3294
Size: 92 1443 127 293 160 238 941
Fragments arranged by size:
1443 941 293 238 160 127 92
BsaWI w'CCGG_w
Cuts at: 0 110 281 687 1619 2129 3294
Size: 110 171 406 932 510 1165
Fragments arranged by size:
1165 932 510 406 171 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2899 2929 3294
Size: 2899 30 365
Fragments arranged by size:
2899 365 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1285 1804 2071 2155 2254 2593 3294
Size: 1285 519 267 84 99 339 701
Fragments arranged by size:
1285 701 519 339 267 99 84
BseYI C'CCAG_C
Cuts at: 0 340 799 927 986 1666 2648 3110 3294
Size: 340 459 128 59 680 982 462 184
Fragments arranged by size:
982 680 462 459 340 184 128 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1300 3294
Size: 1300 1994
BsiEI CG_ry'CG
Cuts at: 0 204 2316 2724 3294
Size: 204 2112 408 570
Fragments arranged by size:
2112 570 408 204
BsiHKAI G_wGCw'C
Cuts at: 0 161 1732 2014 2711 2767 3008 3294
Size: 161 1571 282 697 56 241 286
Fragments arranged by size:
1571 697 286 282 241 161 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1556 1912 3294
Size: 1556 356 1382
Fragments arranged by size:
1556 1382 356
Bsp1286I G_dGCh'C
Cuts at: 0 161 1070 1360 1732 1991 2014 2711 2767
Size: 161 909 290 372 259 23 697 56
Cuts at: 2767 2876 2896 2929 2950 2991 3008 3144 3294
Size: 109 20 33 21 41 17 136 150
Fragments arranged by size:
909 697 372 290 259 161 150 136
109 56 41 33 23 21 20 17
BspEI T'CCGG_A
Cuts at: 0 110 1619 2129 3294
Size: 110 1509 510 1165
Fragments arranged by size:
1509 1165 510 110
BspHI T'CATG_A
Cuts at: 0 680 1370 3294
Size: 680 690 1924
Fragments arranged by size:
1924 690 680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 771 932 1973 3294
Size: 771 161 1041 1321
Fragments arranged by size:
1321 1041 771 161
BsrBI CCG'CTC
Cuts at: 0 50 2187 2244 2280 3294
Size: 50 2137 57 36 1014
Fragments arranged by size:
2137 1014 57 50 36
BsrDI GCAATG_nn'
Cuts at: 0 95 2871 3294
Size: 95 2776 423
Fragments arranged by size:
2776 423 95
BsrFI r'CCGG_y
Cuts at: 0 1492 1675 1855 3046 3242 3294
Size: 1492 183 180 1191 196 52
Fragments arranged by size:
1492 1191 196 183 180 52
BssSI C'ACGA_G
Cuts at: 0 826 1093 1468 3294
Size: 826 267 375 1826
Fragments arranged by size:
1826 826 375 267
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1446 3294
Size: 1446 1848
BstEII G'GTnAC_C
Cuts at: 0 2301 3294
Size: 2301 993
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1186 1516 1707 2856 2964 3294
Size: 1186 330 191 1149 108 330
Fragments arranged by size:
1186 1149 330 330 191 108
BstYI r'GATC_y
Cuts at: 0 518 557 791 836 1172 3206 3219 3294
Size: 518 39 234 45 336 2034 13 75
Fragments arranged by size:
2034 518 336 234 75 45 39 13
BtgI C'CryG_G
Cuts at: 0 1409 1927 2685 3294
Size: 1409 518 758 609
Fragments arranged by size:
1409 758 609 518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2762 3294
Size: 2762 532
BtsI GCAGTG_nn'
Cuts at: 0 1432 2711 3294
Size: 1432 1279 583
Fragments arranged by size:
1432 1279 583
DraIII CAC_nnn'GTG
Cuts at: 0 39 1812 3294
Size: 39 1773 1482
Fragments arranged by size:
1773 1482 39
EaeI y'GGCC_r
Cuts at: 0 1058 1712 1924 2789 3044 3079 3252 3294
Size: 1058 654 212 865 255 35 173 42
Fragments arranged by size:
1058 865 654 255 212 173 42 35
EarI CTCTTCn'nnn_
Cuts at: 0 1087 1127 1576 1633 3294
Size: 1087 40 449 57 1661
Fragments arranged by size:
1661 1087 449 57 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 410 2984 3294
Size: 410 2574 310
Fragments arranged by size:
2574 410 310
EcoICRI GAG'CTC
Cuts at: 0 2765 3294
Size: 2765 529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1276 1506 1849 2074 2257 2548 2595
Size: 595 681 230 343 225 183 291 47
Cuts at: 2595 2926 2939 3294
Size: 331 13 355
Fragments arranged by size:
681 595 355 343 331 291 230 225
183 47 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 1060 1096 2053 2056 2503 3294
Size: 220 840 36 957 3 447 791
Fragments arranged by size:
957 840 791 447 220 36 3
EcoO109I rG'GnC_Cy
Cuts at: 0 647 1463 1775 2398 2594 2892 2893 2946
Size: 647 816 312 623 196 298 1 53
Cuts at: 2946 2947 3141 3261 3294
Size: 1 194 120 33
Fragments arranged by size:
816 647 623 312 298 196 194 120
53 33 1 1
EcoRI G'AATT_C
Cuts at: 0 3016 3294
Size: 3016 278
EcoRV GAT'ATC
Cuts at: 0 426 3294
Size: 426 2868
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 33 2358 3294
Size: 33 2325 936
Fragments arranged by size:
2325 936 33
FspI TGC'GCA
Cuts at: 0 481 1849 3294
Size: 481 1368 1445
Fragments arranged by size:
1445 1368 481
HaeII r_GCGC'y
Cuts at: 0 18 120 1493 1665 2118 2574 3294
Size: 18 102 1373 172 453 456 720
Fragments arranged by size:
1373 720 456 453 172 102 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2899 2931 3111 3143 3294
Size: 2899 32 180 32 151
Fragments arranged by size:
2899 180 151 32 32
HincII GTy'rAC
Cuts at: 0 1980 3294
Size: 1980 1314
HindIII A'AGCT_T
Cuts at: 0 2156 3294
Size: 2156 1138
KasI G'GCGC_C
Cuts at: 0 1661 2114 3294
Size: 1661 453 1180
Fragments arranged by size:
1661 1180 453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 587 1601 2186 2704 3294
Size: 464 123 1014 585 518 590
Fragments arranged by size:
1014 590 585 518 464 123
MscI TGG'CCA
Cuts at: 0 1060 1714 2791 3294
Size: 1060 654 1077 503
Fragments arranged by size:
1077 1060 654 503
MslI CAynn'nnrTG
Cuts at: 0 1012 1147 1720 1843 3294
Size: 1012 135 573 123 1451
Fragments arranged by size:
1451 1012 573 135 123
MspA1I CmG'CkG
Cuts at: 0 447 636 813 941 986 1295 1484 1541
Size: 447 189 177 128 45 309 189 57
Cuts at: 1541 1748 1929 2687 3294
Size: 207 181 758 607
Fragments arranged by size:
758 607 447 309 207 189 189 181
177 128 57 45
NaeI GCC'GGC
Cuts at: 0 1494 1677 1857 3048 3244 3294
Size: 1494 183 180 1191 196 50
Fragments arranged by size:
1494 1191 196 183 180 50
NarI GG'CG_CC
Cuts at: 0 1662 2115 3294
Size: 1662 453 1179
Fragments arranged by size:
1662 1179 453
NgoMIV G'CCGG_C
Cuts at: 0 1492 1675 1855 3046 3242 3294
Size: 1492 183 180 1191 196 52
Fragments arranged by size:
1492 1191 196 183 180 52
NspI r_CATG'y
Cuts at: 0 279 291 3054 3294
Size: 279 12 2763 240
Fragments arranged by size:
2763 279 240 12
PasI CC'CwG_GG
Cuts at: 0 2964 3294
Size: 2964 330
PciI A'CATG_T
Cuts at: 0 275 3294
Size: 275 3019
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 813 3042 3088 3156 3168 3294
Size: 639 174 2229 46 68 12 126
Fragments arranged by size:
2229 639 174 126 68 46 12
PfoI T'CCnGG_A
Cuts at: 0 2051 2525 3294
Size: 2051 474 769
Fragments arranged by size:
2051 769 474
PmlI CAC'GTG
Cuts at: 0 2121 3294
Size: 2121 1173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 508 540 2059 2091 3294
Size: 508 32 1519 32 1203
Fragments arranged by size:
1519 1203 508 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1463 2398 3294
Size: 1463 935 896
Fragments arranged by size:
1463 935 896
PspOMI G'GGCC_C
Cuts at: 0 2892 2946 3140 3294
Size: 2892 54 194 154
Fragments arranged by size:
2892 194 154 54
PspXI vC'TCGA_Gb
Cuts at: 0 451 3294
Size: 451 2843
PstI C_TGCA'G
Cuts at: 0 437 824 953 1232 1442 1451 1895 3294
Size: 437 387 129 279 210 9 444 1399
Fragments arranged by size:
1399 444 437 387 279 210 129 9
PvuII CAG'CTG
Cuts at: 0 636 813 1484 3294
Size: 636 177 671 1810
Fragments arranged by size:
1810 671 636 177
SacI G_AGCT'C
Cuts at: 0 2767 3294
Size: 2767 527
SacII CC_GC'GG
Cuts at: 0 1930 2688 3294
Size: 1930 758 606
Fragments arranged by size:
1930 758 606
SanDI GG'GwC_CC
Cuts at: 0 1463 3294
Size: 1463 1831
SapI GCTCTTCn'nnn_
Cuts at: 0 1633 3294
Size: 1633 1661
ScaI AGT'ACT
Cuts at: 0 511 1312 3294
Size: 511 801 1982
Fragments arranged by size:
1982 801 511
SexAI A'CCwGG_T
Cuts at: 0 725 1706 1808 3294
Size: 725 981 102 1486
Fragments arranged by size:
1486 981 725 102
SfcI C'TryA_G
Cuts at: 0 433 820 949 1228 1438 1447 1891 2623
Size: 433 387 129 279 210 9 444 732
Cuts at: 2623 3294
Size: 671
Fragments arranged by size:
732 671 444 433 387 279 210 129
9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3260 3294
Size: 3260 34
SfoI GGC'GCC
Cuts at: 0 1663 2116 3294
Size: 1663 453 1178
Fragments arranged by size:
1663 1178 453
SmaI CCC'GGG
Cuts at: 0 2830 2944 2986 3294
Size: 2830 114 42 308
Fragments arranged by size:
2830 308 114 42
SmlI C'TyrA_G
Cuts at: 0 451 2710 3294
Size: 451 2259 584
Fragments arranged by size:
2259 584 451
SphI G_CATG'C
Cuts at: 0 3054 3294
Size: 3054 240
StuI AGG'CCT
Cuts at: 0 457 1612 2107 2937 3294
Size: 457 1155 495 830 357
Fragments arranged by size:
1155 830 495 457 357
StyI C'CwwG_G
Cuts at: 0 383 1061 2015 2792 3294
Size: 383 678 954 777 502
Fragments arranged by size:
954 777 678 502 383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2289 2560 2944 3294
Size: 2289 271 384 350
Fragments arranged by size:
2289 384 350 271
TatI w'GTAC_w
Cuts at: 0 509 1136 1310 3294
Size: 509 627 174 1984
Fragments arranged by size:
1984 627 509 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1375 1407 3266 3294
Size: 1375 32 1859 28
Fragments arranged by size:
1859 1375 32 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 570 972 1513 1707 3294
Size: 366 204 402 541 194 1587
Fragments arranged by size:
1587 541 402 366 204 194
XhoI C'TCGA_G
Cuts at: 0 451 3294
Size: 451 2843
XmaI C'CCGG_G
Cuts at: 0 2828 2942 2984 3294
Size: 2828 114 42 310
Fragments arranged by size:
2828 310 114 42
XmnI GAAnn'nnTTC
Cuts at: 0 118 3294
Size: 118 3176
ZraI GAC'GTC
Cuts at: 0 1536 3294
Size: 1536 1758
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuII SacI SacII SanDI SapI ScaI
SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AleI AloI AscI
AseI AsiSI AvrII BaeI BciVI BclI BfrBI BmtI
BsaBI BsiWI BsmI BsrGI BssHII BstBI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI FseI FspAI HpaI
KpnI MfeI MluI NcoI NdeI NheI NotI NruI
NsiI PacI PmeI PshAI PsiI PsrI PvuI RsrII
SalI SbfI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI