(Circular) (Six-base) MAPSORT of: pFN21ASDB0060.seq Check: 3949 from: 1 to: 7450 pFN21ASDB0060 7450 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5260 278 Size: 53 83 186 936 3724 2468 Fragments arranged by size: 3724 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3413 4793 1937 Size: 1476 1380 4594 Fragments arranged by size: 4594 1476 1380 Acc65I G'GTAC_C Cuts at: 3714 4715 4771 3714 Size: 1001 56 6393 Fragments arranged by size: 6393 1001 56 AclI AA'CG_TT Cuts at: 1563 2052 5577 5950 1563 Size: 489 3525 373 3063 Fragments arranged by size: 3525 3063 489 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3176 3919 4361 4639 5513 6897 7165 1632 Size: 1544 743 442 278 874 1384 268 1917 Fragments arranged by size: 1917 1544 1384 874 743 442 278 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7253 AflIII A'CryG_T Cuts at: 1203 4074 4740 6349 7326 1203 Size: 2871 666 1609 977 1327 Fragments arranged by size: 2871 1609 1327 977 666 AgeI A'CCGG_T Cuts at: 6281 6281 Size: 7450 AhdI GACnn_n'nnGTC Cuts at: 2041 6179 2041 Size: 4138 3312 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7450 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7450 AlwNI CAG_nnn'CTG Cuts at: 2068 2234 5193 6765 2068 Size: 166 2959 1572 2753 Fragments arranged by size: 2959 2753 1572 166 ApaLI G'TGCA_C Cuts at: 5507 6663 5507 Size: 1156 6294 ApoI r'AATT_y Cuts at: 1493 2174 3085 3356 3493 3819 4526 4759 4985 Size: 681 911 271 137 326 707 233 226 Cuts at: 4985 5137 7246 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 911 707 681 326 271 233 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 6004 160 Size: 872 4972 1606 Fragments arranged by size: 4972 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7450 AvaI C'yCGr_G Cuts at: 1870 1943 3981 4775 1870 Size: 73 2038 794 4545 Fragments arranged by size: 4545 2038 794 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4573 4606 4573 Size: 33 7417 BamHI G'GATC_C Cuts at: 2079 4780 5147 2079 Size: 2701 367 4382 Fragments arranged by size: 4382 2701 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3117 3714 4715 4771 6226 Size: 325 217 627 1330 597 1001 56 1455 Cuts at: 6226 618 Size: 1842 Fragments arranged by size: 1842 1455 1330 1001 627 597 325 217 56 BanII G_rGCy'C Cuts at: 1391 2483 4695 4769 1391 Size: 1092 2212 74 4072 Fragments arranged by size: 4072 2212 1092 74 BbeI G_GCGC'C Cuts at: 3121 3121 Size: 7450 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3164 3925 4631 928 Size: 971 1265 761 706 3747 Fragments arranged by size: 3747 1265 971 761 706 BbvCI CC'TCA_GC Cuts at: 3214 3278 3214 Size: 64 7386 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2038 2072 2131 2165 5641 5675 1903 Size: 34 101 34 59 34 3476 34 3678 Fragments arranged by size: 3678 3476 101 59 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2728 5342 6552 2728 Size: 2614 1210 3626 Fragments arranged by size: 3626 2614 1210 BclI T'GATC_A Cuts at: 1548 3517 4360 1548 Size: 1969 843 4638 Fragments arranged by size: 4638 1969 843 BfrBI ATG'CAT Cuts at: 3608 3608 Size: 7450 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6061 136 Size: 107 122 71 631 605 4389 1525 Fragments arranged by size: 4389 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7445 7445 Size: 7450 BlpI GC'TnA_GC Cuts at: 2315 4867 2315 Size: 2552 4898 Bme1580I G_kGCm'C Cuts at: 1790 2467 2530 4333 5511 6667 1790 Size: 677 63 1803 1178 1156 2573 Fragments arranged by size: 2573 1803 1178 1156 677 63 BmgBI CAC'GTC Cuts at: 1318 3889 1318 Size: 2571 4879 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2461 2685 5181 Size: 416 53 83 399 24 1044 224 2496 Cuts at: 5181 6139 442 Size: 958 1753 Fragments arranged by size: 2496 1753 1044 958 416 399 224 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7450 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3229 3229 Size: 7450 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2571 3894 3920 4045 4309 6110 1239 Size: 1332 1323 26 125 264 1801 2579 Fragments arranged by size: 2579 1801 1332 1323 264 125 26 Bpu10I CC'TnA_GC Cuts at: 2379 2444 3214 3278 2379 Size: 65 770 64 6551 Fragments arranged by size: 6551 770 65 64 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3116 3359 4371 5575 6440 6738 3116 Size: 243 1012 1204 865 298 3828 Fragments arranged by size: 3828 1204 1012 865 298 243 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3237 3264 3635 3910 6113 882 Size: 617 1738 27 371 275 2203 2219 Fragments arranged by size: 2219 2203 1738 617 371 275 27 BsaAI yAC'GTr Cuts at: 493 1204 4743 4747 5241 493 Size: 711 3539 4 494 2702 Fragments arranged by size: 3539 2702 711 494 4 BsaBI GATnn'nnATC Cuts at: 4254 7444 4254 Size: 3190 4260 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3118 5257 5639 6274 Size: 53 83 186 936 1585 2139 382 635 Cuts at: 6274 275 Size: 1451 Fragments arranged by size: 2139 1585 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5883 6281 6555 6702 7113 5883 Size: 398 274 147 411 6220 Fragments arranged by size: 6220 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2544 2574 4307 4337 1178 Size: 30 165 30 1141 30 1733 30 4291 Fragments arranged by size: 4291 1733 1141 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2552 2968 4517 1186 Size: 1366 416 1549 4119 Fragments arranged by size: 4119 1549 1366 416 BseYI C'CCAG_C Cuts at: 2109 6653 7149 2109 Size: 4544 496 2410 Fragments arranged by size: 4544 2410 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3287 4109 4254 4616 1163 Size: 573 1551 822 145 362 3997 Fragments arranged by size: 3997 1551 822 573 362 145 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4900 5661 6689 1540 Size: 275 186 2899 761 1028 2301 Fragments arranged by size: 2899 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2483 4556 5511 5596 6667 2483 Size: 2073 955 85 1071 3266 Fragments arranged by size: 3266 2073 1071 955 85 BsiWI C'GTAC_G Cuts at: 4743 4743 Size: 7450 BsmI GAATG_Cn' Cuts at: 4387 4961 5054 4387 Size: 574 93 6783 Fragments arranged by size: 6783 574 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4420 4420 Size: 7450 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2467 2483 2530 4333 4556 4695 4769 Size: 399 677 16 47 1803 223 139 74 Cuts at: 4769 5511 5596 6667 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1803 1071 742 677 399 223 139 85 74 47 16 BspEI T'CCGG_A Cuts at: 7113 7113 Size: 7450 BspHI T'CATG_A Cuts at: 3514 5339 7069 3514 Size: 1825 1730 3895 Fragments arranged by size: 3895 1825 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3726 4805 844 Size: 2882 1079 3489 Fragments arranged by size: 3489 2882 1079 BsrBI CCG'CTC Cuts at: 1394 1823 2967 5337 1394 Size: 429 1144 2370 3507 Fragments arranged by size: 3507 2370 1144 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3711 4087 4263 5945 6119 65 Size: 1183 2463 376 176 1682 174 1396 Fragments arranged by size: 2463 1682 1396 1183 376 176 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6094 6281 1700 Size: 110 4284 187 2869 Fragments arranged by size: 4284 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7450 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7450 BssSI C'ACGA_G Cuts at: 3284 4274 5510 6522 3284 Size: 990 1236 1012 4212 Fragments arranged by size: 4212 1236 1012 990 BstAPI GCAn_nnn'nTGC Cuts at: 794 2234 794 Size: 1440 6010 BstBI TT'CG_AA Cuts at: 2007 6254 2007 Size: 4247 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7450 BstYI r'GATC_y Cuts at: 1972 2079 3373 3870 4780 5147 5532 5549 7001 Size: 107 1294 497 910 367 385 17 1452 Cuts at: 7001 7087 7099 7445 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1294 910 497 385 367 346 107 86 17 12 BstZ17I GTA'TAC Cuts at: 3414 3414 Size: 7450 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5941 Fragments arranged by size: 5941 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6711 Fragments arranged by size: 6711 683 56 BtsI GCAGTG_nn' Cuts at: 2506 3181 4966 5760 5780 2506 Size: 675 1785 794 20 4176 Fragments arranged by size: 4176 1785 794 675 20 ClaI AT'CG_AT Cuts at: 1554 3031 3428 1554 Size: 1477 397 5576 Fragments arranged by size: 5576 1477 397 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7415 DraI TTT'AAA Cuts at: 4338 4531 4754 4338 Size: 193 223 7034 Fragments arranged by size: 7034 223 193 DraIII CAC_nnn'GTG Cuts at: 4279 4279 Size: 7450 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2894 6457 809 Size: 507 1578 3563 1802 Fragments arranged by size: 3563 1802 1578 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4897 5786 7415 Size: 54 1425 50 275 18 3067 889 1629 Cuts at: 7415 8 Size: 43 Fragments arranged by size: 3067 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4897 1537 Size: 275 3085 4090 Fragments arranged by size: 4090 3085 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2215 4437 4494 5380 1353 Size: 862 2222 57 886 3423 Fragments arranged by size: 3423 2222 886 862 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6037 6411 6557 387 Size: 1064 138 4448 374 146 1280 Fragments arranged by size: 4448 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2481 2481 Size: 7450 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2571 3176 3894 3919 3920 4045 4309 Size: 393 939 605 718 25 1 125 264 Cuts at: 4309 4361 4639 5513 6110 6897 7165 1239 Size: 52 278 874 597 787 268 1524 Fragments arranged by size: 1524 939 874 787 718 605 597 393 278 268 264 125 52 25 1 EcoNI CCTnn'n_nnAGG Cuts at: 2324 2324 Size: 7450 EcoO109I rG'GnC_Cy Cuts at: 2329 3469 2329 Size: 1140 6310 EcoRI G'AATT_C Cuts at: 3819 4759 5137 3819 Size: 940 378 6132 Fragments arranged by size: 6132 940 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2895 3700 2895 Size: 805 6645 FspI TGC'GCA Cuts at: 5956 5956 Size: 7450 HaeII r_GCGC'y Cuts at: 1678 3121 6597 1678 Size: 1443 3476 2531 Fragments arranged by size: 3476 2531 1443 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2387 2419 3124 Size: 32 135 32 455 32 328 32 705 Cuts at: 3124 3156 3439 3471 3784 3816 3915 3947 4307 Size: 32 283 32 313 32 99 32 360 Cuts at: 4307 4339 6091 6123 6165 6197 1373 Size: 32 1752 32 42 32 2626 Fragments arranged by size: 2626 1752 705 455 360 328 313 283 135 99 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3955 4794 5040 669 Size: 988 281 2017 839 246 3079 Fragments arranged by size: 3079 2017 988 839 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7450 HpaI GTT'AAC Cuts at: 5040 5040 Size: 7450 KasI G'GCGC_C Cuts at: 3117 3117 Size: 7450 KpnI G_GTAC'C Cuts at: 3718 4719 4775 3718 Size: 1001 56 6393 Fragments arranged by size: 6393 1001 56 MfeI C'AATT_G Cuts at: 3963 5049 3963 Size: 1086 6364 MluI A'CGCG_T Cuts at: 7326 7326 Size: 7450 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3401 3639 6564 6748 1305 Size: 2096 238 2925 184 2007 Fragments arranged by size: 2925 2096 2007 238 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5628 Fragments arranged by size: 5628 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2822 5408 5767 5926 518 Size: 648 1656 2586 359 159 2042 Fragments arranged by size: 2586 2042 1656 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3218 3318 3833 4305 4866 5543 6691 6936 Size: 1612 100 515 472 561 677 1148 245 Cuts at: 6936 1606 Size: 2120 Fragments arranged by size: 2120 1612 1148 677 561 515 472 245 100 NarI GG'CG_CC Cuts at: 3118 3118 Size: 7450 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5941 Fragments arranged by size: 5941 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7450 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7450 NotI GC'GGCC_GC Cuts at: 4897 4897 Size: 7450 NsiI A_TGCA'T Cuts at: 3610 3610 Size: 7450 NspI r_CATG'y Cuts at: 1132 1229 3256 3700 4078 4105 4808 6353 1132 Size: 97 2027 444 378 27 703 1545 2229 Fragments arranged by size: 2229 2027 1545 703 444 378 97 27 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7450 PciI A'CATG_T Cuts at: 4074 6349 4074 Size: 2275 5175 PflMI CCAn_nnn'nTGG Cuts at: 1108 4252 1108 Size: 3144 4306 PfoI T'CCnGG_A Cuts at: 2587 2720 2587 Size: 133 7317 PmeI GTTT'AAAC Cuts at: 4754 4754 Size: 7450 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5519 5551 7032 7064 5519 Size: 32 1481 32 5905 Fragments arranged by size: 5905 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2329 3469 2329 Size: 1140 6310 PshAI GACnn'nnGTC Cuts at: 1613 4635 1613 Size: 3022 4428 PsiI TTA'TAA Cuts at: 3743 5020 3743 Size: 1277 6173 PstI C_TGCA'G Cuts at: 830 2064 2078 3562 3583 4802 830 Size: 1234 14 1484 21 1219 3478 Fragments arranged by size: 3478 1484 1234 1219 21 14 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7450 PvuII CAG'CTG Cuts at: 3218 3218 Size: 7450 SacI G_AGCT'C Cuts at: 2483 2483 Size: 7450 SalI G'TCGA_C Cuts at: 1936 4792 1936 Size: 2856 4594 SbfI CC_TGCA'GG Cuts at: 4802 4802 Size: 7450 ScaI AGT'ACT Cuts at: 5698 7410 5698 Size: 1712 5738 SfcI C'TryA_G Cuts at: 826 1045 2060 2074 3558 3579 4798 5933 6614 Size: 219 1015 14 1484 21 1219 1135 681 Cuts at: 6614 6805 826 Size: 191 1471 Fragments arranged by size: 1484 1471 1219 1135 1015 681 219 191 21 14 SfoI GGC'GCC Cuts at: 3119 3119 Size: 7450 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7450 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7450 SmaI CCC'GGG Cuts at: 1872 4777 1872 Size: 2905 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3095 3338 3981 4386 5554 6455 Size: 197 926 1152 243 643 405 1168 901 Cuts at: 6455 6717 820 Size: 262 1553 Fragments arranged by size: 1553 1168 1152 926 901 643 405 262 243 197 SnaBI TAC'GTA Cuts at: 493 4747 5241 493 Size: 4254 494 2702 Fragments arranged by size: 4254 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7450 SphI G_CATG'C Cuts at: 1132 4808 1132 Size: 3676 3774 SspI AAT'ATT Cuts at: 5 52 4569 5374 5 Size: 47 4517 805 2081 Fragments arranged by size: 4517 2081 805 47 StuI AGG'CCT Cuts at: 1517 2384 2428 1517 Size: 867 44 6539 Fragments arranged by size: 6539 867 44 StyI C'CwwG_G Cuts at: 513 1065 2022 3187 4889 513 Size: 552 957 1165 1702 3074 Fragments arranged by size: 3074 1702 1165 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5477 5494 5647 5832 6277 325 Size: 1255 3897 17 153 185 445 1498 Fragments arranged by size: 3897 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3678 5696 7408 Size: 274 80 33 51 1443 1701 2018 1712 Cuts at: 7408 96 Size: 138 Fragments arranged by size: 2018 1712 1701 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2544 2576 3187 3219 7410 7442 2544 Size: 32 611 32 4191 32 2552 Fragments arranged by size: 4191 2552 611 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2716 3553 1316 Size: 1400 837 5213 Fragments arranged by size: 5213 1400 837 XbaI T'CTAG_A Cuts at: 3823 4786 3823 Size: 963 6487 XhoI C'TCGA_G Cuts at: 1943 3981 1943 Size: 2038 5412 XmaI C'CCGG_G Cuts at: 1870 4775 1870 Size: 2905 4545 XmnI GAAnn'nnTTC Cuts at: 5579 5579 Size: 7450 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5258 276 Size: 53 83 186 936 3724 2468 Fragments arranged by size: 3724 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BstXI Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI