Restriction map for
pFN21ASDB0057
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BciVI BclI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 1259 GGTACC G^GTACC
AccI 523 GTCTAC GT^[AC][GT]AC
ApaI 1269 GGGCCC GGGCC^C
Asp700I 451 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1259 GGTACC G^GTACC
BcgI 1644 GCAGAAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 493 CTAG C^TAG
BglII 440 AGATCT A^GATCT
BsaI 461 GAGACC GGTCTC(1/5)
BsgI 675 GTGCAG GTGCAG(16/14)
BspHI 1937 TCATGA T^CATGA
BsrDI 2089 GCAATG GCAATG(2/0)
BstAPI 1458 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 123 GGTGACC G^GT[ACGT]ACC
BstPI 123 GGTGACC G^GT[ACGT]ACC
BstXI 2084 CCATGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 123 GGTGACC G^GT[ACGT]ACC
KpnI 1259 GGTACC GGTAC^C
MaeI 493 CTAG C^TAG
NaeI 1999 GCCGGC GCC^GGC
NgoMIV 1999 GCCGGC G^CCGGC
PshAI 516 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1937 TCATGA T^CATGA
SacI 1705 GAGCTC GAGCT^C
SapI 1218 GAAGAGC GCTCTTC(1/4)
SbfI 1380 CCTGCAGG CCTGCA^GG
SexAI 416 ACCTGGT A^CC[AT]GGT
SfiI 18 GGCCAGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1380 CCTGCAGG CCTGCA^GG
TfiI 456 GATTC G^A[AT]TC
XmnI 451 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 1144 GAATTC [GA]^AATT[CT]
2173 GAATTC
AocI 859 CCTAAGG CC^T[ACGT]AGG
1264 CCTGAGG
ApoI 1144 GAATTC [GA]^AATT[CT]
2173 GAATTC
BbvCI 57 CCTCAGC CCTCAGC(-5/-2)
2299 CCTCAGC
BsmI 363 GAATGC GAATGC(1/-1)
996 GAATGC
BsrFI 1943 ACCGGC [GA]^CCGG[CT]
1999 GCCGGC
Bsu36I 859 CCTAAGG CC^T[ACGT]AGG
1264 CCTGAGG
Cfr10I 1943 ACCGGC [GA]^CCGG[CT]
1999 GCCGGC
Eco81I 859 CCTAAGG CC^T[ACGT]AGG
1264 CCTGAGG
EcoNI 1728 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1845 CCTGGAGGAGG
EcoRI 1144 GAATTC G^AATTC
2173 GAATTC
NspI 576 GCATGT [GA]CATG^[CT]
2325 ACATGC
PflMI 1983 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2279 CCAGATCCTGG
PvuII 1177 CAGCTG CAG^CTG
2156 CAGCTG
TaqI 459 TCGA T^CGA
1901 TCGA
TthHB8I 459 TCGA T^CGA
1901 TCGA
Van91I 1983 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2279 CCAGATCCTGG
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TseI Tsp45I Tsp509I TspRI XcmI
XhoII XmaCI XmaI