(Circular) (Six-base) MAPSORT of: pFN21ASDB0057.seq Check: 2776 from: 1 to: 7108 pFN21ASDB0057 7108 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4918 278 Size: 53 83 186 936 3382 2468 Fragments arranged by size: 3382 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2547 4451 1937 Size: 610 1904 4594 Fragments arranged by size: 4594 1904 610 Acc65I G'GTAC_C Cuts at: 3282 4429 3282 Size: 1147 5961 AclI AA'CG_TT Cuts at: 1563 5235 5608 1563 Size: 3672 373 3063 Fragments arranged by size: 3672 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3212 3665 5171 6555 6823 1632 Size: 1580 453 1506 1384 268 1917 Fragments arranged by size: 1917 1580 1506 1384 453 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6911 AflIII A'CryG_T Cuts at: 1203 6007 6984 1203 Size: 4804 977 1327 Fragments arranged by size: 4804 1327 977 AgeI A'CCGG_T Cuts at: 5939 5939 Size: 7108 AhdI GACnn_n'nnGTC Cuts at: 5837 5837 Size: 7108 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7108 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3675 1184 Size: 2491 4617 AlwNI CAG_nnn'CTG Cuts at: 2119 2440 3487 3868 4012 4308 4851 6423 2119 Size: 321 1047 381 144 296 543 1572 2804 Fragments arranged by size: 2804 1572 1047 543 381 321 296 144 ApaI G_GGCC'C Cuts at: 3296 3296 Size: 7108 ApaLI G'TGCA_C Cuts at: 5165 6321 5165 Size: 1156 5952 ApoI r'AATT_y Cuts at: 1493 3167 4196 4417 4643 4795 6904 1493 Size: 1674 1029 221 226 152 2109 1697 Fragments arranged by size: 2109 1697 1674 1029 226 221 152 AseI AT'TA_AT Cuts at: 160 1032 5662 160 Size: 872 4630 1606 Fragments arranged by size: 4630 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7108 AvaI C'yCGr_G Cuts at: 1870 1943 2173 2363 2534 2625 2827 3129 4098 Size: 73 230 190 171 91 202 302 969 Cuts at: 4098 4433 1870 Size: 335 4545 Fragments arranged by size: 4545 969 335 302 230 202 190 171 91 73 BamHI G'GATC_C Cuts at: 4438 4805 4438 Size: 367 6741 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 3282 3737 4015 4311 Size: 325 217 627 283 1212 455 278 296 Cuts at: 4311 4429 5884 618 Size: 118 1455 1842 Fragments arranged by size: 1842 1455 1212 627 455 325 296 283 278 217 118 BanII G_rGCy'C Cuts at: 1391 2364 3296 3732 4427 1391 Size: 973 932 436 695 4072 Fragments arranged by size: 4072 973 932 695 436 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6137 BbvCI CC'TCA_GC Cuts at: 2081 4323 2081 Size: 2242 4866 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3656 3690 5299 5333 1903 Size: 34 1719 34 1609 34 3678 Fragments arranged by size: 3678 1719 1609 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5000 6210 5000 Size: 1210 5898 BclI T'GATC_A Cuts at: 1548 1548 Size: 7108 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 2319 3314 Size: 107 122 71 631 605 376 271 995 Cuts at: 3314 4358 5719 136 Size: 1044 1361 1525 Fragments arranged by size: 1525 1361 1044 995 631 605 376 271 122 107 71 BglII A'GATC_T Cuts at: 2463 7103 2463 Size: 4640 2468 BlpI GC'TnA_GC Cuts at: 4525 4525 Size: 7108 Bme1580I G_kGCm'C Cuts at: 1790 2324 3296 3740 5169 6325 1790 Size: 534 972 444 1429 1156 2573 Fragments arranged by size: 2573 1429 1156 972 534 444 BmgBI CAC'GTC Cuts at: 1318 2131 4284 1318 Size: 813 2153 4142 Fragments arranged by size: 4142 2153 813 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2147 3056 3290 Size: 416 53 83 399 24 730 909 234 Cuts at: 3290 4839 5797 442 Size: 1549 958 1753 Fragments arranged by size: 1753 1549 958 909 730 416 399 234 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7108 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2507 2677 3097 2507 Size: 170 420 6518 Fragments arranged by size: 6518 420 170 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2264 2984 3224 3389 3446 3512 3533 3890 Size: 1025 720 240 165 57 66 21 357 Cuts at: 3890 4161 4178 5768 1239 Size: 271 17 1590 2579 Fragments arranged by size: 2579 1590 1025 720 357 271 240 165 66 57 21 17 Bpu10I CC'TnA_GC Cuts at: 2081 4323 2081 Size: 2242 4866 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2039 3770 3887 3893 5233 6098 6396 2039 Size: 1731 117 6 1340 865 298 2751 Fragments arranged by size: 2751 1731 1340 865 298 117 6 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2478 5771 882 Size: 617 979 3293 2219 Fragments arranged by size: 3293 2219 979 617 BsaAI yAC'GTr Cuts at: 493 1204 4405 4899 493 Size: 711 3201 494 2702 Fragments arranged by size: 3201 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7102 7102 Size: 7108 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2205 2544 4026 4915 Size: 53 83 186 936 672 339 1482 889 Cuts at: 4915 5297 5932 275 Size: 382 635 1451 Fragments arranged by size: 1482 1451 936 889 672 635 382 339 186 83 53 BsaWI w'CCGG_w Cuts at: 5541 5939 6213 6360 6771 5541 Size: 398 274 147 411 5878 Fragments arranged by size: 5878 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2477 2507 2549 2579 2963 Size: 30 165 30 1074 30 42 30 384 Cuts at: 2963 2993 3980 4010 1178 Size: 30 987 30 4276 Fragments arranged by size: 4276 1074 987 384 165 42 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2554 3191 3338 3443 3446 3488 3509 Size: 922 446 637 147 105 3 42 21 Cuts at: 3509 3575 3731 3887 3959 4001 4004 1186 Size: 66 156 156 72 42 3 4290 Fragments arranged by size: 4290 922 637 446 156 156 147 105 72 66 42 42 21 3 3 BseYI C'CCAG_C Cuts at: 3198 3220 3357 6311 6807 3198 Size: 22 137 2954 496 3499 Fragments arranged by size: 3499 2954 496 137 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2719 1163 Size: 573 983 5552 Fragments arranged by size: 5552 983 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4558 5319 6347 1540 Size: 275 186 2557 761 1028 2301 Fragments arranged by size: 2557 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2280 2400 2960 3732 5169 5254 6325 2280 Size: 120 560 772 1437 85 1071 3063 Fragments arranged by size: 3063 1437 1071 772 560 120 85 BsmI GAATG_Cn' Cuts at: 2392 3025 4619 4712 2392 Size: 633 1594 93 4788 Fragments arranged by size: 4788 1594 633 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2280 2324 2364 2400 2960 3296 3732 Size: 399 490 44 40 36 560 336 436 Cuts at: 3732 3740 4427 5169 5254 6325 1391 Size: 8 687 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 687 560 490 436 399 336 85 44 40 36 8 BspEI T'CCGG_A Cuts at: 6771 6771 Size: 7108 BspHI T'CATG_A Cuts at: 3960 4997 6727 3960 Size: 1037 1730 4341 Fragments arranged by size: 4341 1730 1037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2496 3411 3528 4463 844 Size: 1652 915 117 935 3489 Fragments arranged by size: 3489 1652 935 915 117 BsrBI CCG'CTC Cuts at: 1394 1823 2421 2667 3421 3682 4995 1394 Size: 429 598 246 754 261 1313 3507 Fragments arranged by size: 3507 1313 754 598 429 261 246 BsrDI GCAATG_nn' Cuts at: 65 1248 4119 5603 5777 65 Size: 1183 2871 1484 174 1396 Fragments arranged by size: 2871 1484 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3966 4022 5752 5939 1700 Size: 110 2156 56 1730 187 2869 Fragments arranged by size: 2869 2156 1730 187 110 56 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7108 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7108 BssSI C'ACGA_G Cuts at: 2108 2753 4224 5168 6180 2108 Size: 645 1471 944 1012 3036 Fragments arranged by size: 3036 1471 1012 944 645 BstAPI GCAn_nnn'nTGC Cuts at: 794 3487 794 Size: 2693 4415 BstBI TT'CG_AA Cuts at: 2007 5912 2007 Size: 3905 3203 BstEII G'GTnAC_C Cuts at: 1184 2146 1184 Size: 962 6146 BstXI CCAn_nnnn'nTGG Cuts at: 4114 4114 Size: 7108 BstYI r'GATC_y Cuts at: 1972 2115 2463 3825 4304 4438 4805 5190 5207 Size: 143 348 1362 479 134 367 385 17 Cuts at: 5207 6659 6745 6757 7103 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1362 479 385 367 348 346 143 134 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2883 3288 2883 Size: 405 6703 BtgI C'CryG_G Cuts at: 513 1065 2022 2124 3570 4107 513 Size: 552 957 102 1446 537 3514 Fragments arranged by size: 3514 1446 957 552 537 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6369 Fragments arranged by size: 6369 683 56 BtsI GCAGTG_nn' Cuts at: 2860 3155 4001 4071 4624 5418 5438 2860 Size: 295 846 70 553 794 20 4530 Fragments arranged by size: 4530 846 794 553 295 70 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7108 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7073 DraI TTT'AAA Cuts at: 4412 4412 Size: 7108 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6115 809 Size: 507 4799 1802 Fragments arranged by size: 4799 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2311 2719 Size: 54 1425 50 275 18 195 286 408 Cuts at: 2719 3555 4052 4555 5444 7073 8 Size: 836 497 503 889 1629 43 Fragments arranged by size: 1629 1425 889 836 503 497 408 286 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4555 1537 Size: 275 2743 4090 Fragments arranged by size: 4090 2743 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 3203 3236 3315 3641 5038 1353 Size: 1116 734 33 79 326 1397 3423 Fragments arranged by size: 3423 1397 1116 734 326 79 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3259 3836 4406 5695 6069 6215 Size: 1064 138 1670 577 570 1289 374 146 Cuts at: 6215 387 Size: 1280 Fragments arranged by size: 1670 1289 1280 1064 577 570 374 146 138 EcoICRI GAG'CTC Cuts at: 3730 3730 Size: 7108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 2984 3212 3224 3389 3446 3512 Size: 393 632 720 228 12 165 57 66 Cuts at: 3512 3533 3665 3890 4161 4178 5171 5768 6555 Size: 21 132 225 271 17 993 597 787 Cuts at: 6555 6823 1239 Size: 268 1524 Fragments arranged by size: 1524 993 787 720 632 597 393 271 268 228 225 165 132 66 57 21 17 12 EcoNI CCTnn'n_nnAGG Cuts at: 3755 3872 3755 Size: 117 6991 EcoO109I rG'GnC_Cy Cuts at: 2050 2259 2349 2557 2792 3142 3292 3517 3865 Size: 209 90 208 235 350 150 225 348 Cuts at: 3865 4119 2050 Size: 254 5039 Fragments arranged by size: 5039 350 348 254 235 225 209 208 150 90 EcoRI G'AATT_C Cuts at: 3167 4196 4417 4795 3167 Size: 1029 221 378 5480 Fragments arranged by size: 5480 1029 378 221 FspI TGC'GCA Cuts at: 5614 5614 Size: 7108 HaeII r_GCGC'y Cuts at: 1678 6255 1678 Size: 4577 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2207 2239 2477 2509 2549 Size: 32 135 32 635 32 238 32 40 Cuts at: 2549 2581 5749 5781 5823 5855 1373 Size: 32 3168 32 42 32 2626 Fragments arranged by size: 3168 2626 635 238 135 42 40 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4452 4698 669 Size: 988 281 2514 246 3079 Fragments arranged by size: 3079 2514 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7108 HpaI GTT'AAC Cuts at: 4698 4698 Size: 7108 KpnI G_GTAC'C Cuts at: 3286 4433 3286 Size: 1147 5961 MfeI C'AATT_G Cuts at: 4707 4707 Size: 7108 MluI A'CGCG_T Cuts at: 6984 6984 Size: 7108 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3618 4143 6222 6406 1305 Size: 2313 525 2079 184 2007 Fragments arranged by size: 2313 2079 2007 525 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2313 2721 10 Size: 54 1425 343 195 286 408 4397 Fragments arranged by size: 4397 1425 408 343 286 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2129 2645 2649 2690 2735 4112 5066 Size: 648 963 516 4 41 45 1377 954 Cuts at: 5066 5425 5584 518 Size: 359 159 2042 Fragments arranged by size: 2042 1377 963 954 648 516 359 159 45 41 4 MspA1I CmG'CkG Cuts at: 1606 2804 3202 4181 4327 4524 5201 6349 6594 Size: 1198 398 979 146 197 677 1148 245 Cuts at: 6594 1606 Size: 2120 Fragments arranged by size: 2120 1198 1148 979 677 398 245 197 146 NaeI GCC'GGC Cuts at: 4024 4024 Size: 7108 NcoI C'CATG_G Cuts at: 513 1065 2022 2124 3570 4107 513 Size: 552 957 102 1446 537 3514 Fragments arranged by size: 3514 1446 957 552 537 102 NdeI CA'TA_TG Cuts at: 387 387 Size: 7108 NgoMIV G'CCGG_C Cuts at: 4022 4022 Size: 7108 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7108 NotI GC'GGCC_GC Cuts at: 4555 4555 Size: 7108 NspI r_CATG'y Cuts at: 1132 1229 2603 4352 4466 6011 1132 Size: 97 1374 1749 114 1545 2229 Fragments arranged by size: 2229 1749 1545 1374 114 97 PasI CC'CwG_GG Cuts at: 1348 2920 3010 1348 Size: 1572 90 5446 Fragments arranged by size: 5446 1572 90 PciI A'CATG_T Cuts at: 6007 6007 Size: 7108 PflMI CCAn_nnn'nTGG Cuts at: 1108 4012 4308 1108 Size: 2904 296 3908 Fragments arranged by size: 3908 2904 296 PfoI T'CCnGG_A Cuts at: 3000 3366 3447 3708 3828 3975 3000 Size: 366 81 261 120 147 6133 Fragments arranged by size: 6133 366 261 147 120 81 PmeI GTTT'AAAC Cuts at: 4412 4412 Size: 7108 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3891 3923 5177 5209 6690 6722 3891 Size: 32 1254 32 1481 32 4277 Fragments arranged by size: 4277 1481 1254 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2349 2557 3142 3517 3865 4119 2349 Size: 208 585 375 348 254 5338 Fragments arranged by size: 5338 585 375 348 254 208 PshAI GACnn'nnGTC Cuts at: 1613 2543 1613 Size: 930 6178 PsiI TTA'TAA Cuts at: 4678 4678 Size: 7108 PspOMI G'GGCC_C Cuts at: 3292 3292 Size: 7108 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2420 2452 2420 Size: 32 7076 PstI C_TGCA'G Cuts at: 830 3408 3756 3810 4066 4460 830 Size: 2578 348 54 256 394 3478 Fragments arranged by size: 3478 2578 394 348 256 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7108 PvuII CAG'CTG Cuts at: 3202 4181 3202 Size: 979 6129 SacI G_AGCT'C Cuts at: 3732 3732 Size: 7108 SalI G'TCGA_C Cuts at: 1936 4450 1936 Size: 2514 4594 SanDI GG'GwC_CC Cuts at: 4119 4119 Size: 7108 SapI GCTCTTCn'nnn_ Cuts at: 3236 3236 Size: 7108 SbfI CC_TGCA'GG Cuts at: 3408 4460 3408 Size: 1052 6056 ScaI AGT'ACT Cuts at: 5356 7068 5356 Size: 1712 5396 SexAI A'CCwGG_T Cuts at: 2439 2439 Size: 7108 SfcI C'TryA_G Cuts at: 826 1045 3404 3752 3806 4062 4456 5591 6272 Size: 219 2359 348 54 256 394 1135 681 Cuts at: 6272 6463 826 Size: 191 1471 Fragments arranged by size: 2359 1471 1135 681 394 348 256 219 191 54 SfiI GGCCn_nnn'nGGCC Cuts at: 2048 2048 Size: 7108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7108 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7108 SmaI CCC'GGG Cuts at: 1872 2175 2627 3131 4435 1872 Size: 303 452 504 1304 4545 Fragments arranged by size: 4545 1304 504 452 303 SmlI C'TyrA_G Cuts at: 820 1017 1943 2054 3785 3902 3908 5212 6113 Size: 197 926 111 1731 117 6 1304 901 Cuts at: 6113 6375 820 Size: 262 1553 Fragments arranged by size: 1731 1553 1304 926 901 262 197 117 111 6 SnaBI TAC'GTA Cuts at: 493 4405 4899 493 Size: 3912 494 2702 Fragments arranged by size: 3912 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7108 SphI G_CATG'C Cuts at: 1132 4466 1132 Size: 3334 3774 SspI AAT'ATT Cuts at: 5 52 5032 5 Size: 47 4980 2081 Fragments arranged by size: 4980 2081 47 StuI AGG'CCT Cuts at: 1517 2881 3308 4081 1517 Size: 1364 427 773 4544 Fragments arranged by size: 4544 1364 773 427 StyI C'CwwG_G Cuts at: 513 1065 2022 2037 2124 2496 3147 3378 3570 Size: 552 957 15 87 372 651 231 192 Cuts at: 3570 4107 4547 513 Size: 537 440 3074 Fragments arranged by size: 3074 957 651 552 537 440 372 231 192 87 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2344 2423 2657 2975 3301 5135 5152 Size: 1255 764 79 234 318 326 1834 17 Cuts at: 5152 5305 5490 5935 325 Size: 153 185 445 1498 Fragments arranged by size: 1834 1498 1255 764 445 326 318 234 185 153 79 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3561 5354 7066 Size: 274 80 33 51 1443 1584 1793 1712 Cuts at: 7066 96 Size: 138 Fragments arranged by size: 1793 1712 1584 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2950 2982 3980 4012 7068 7100 2950 Size: 32 998 32 3056 32 2958 Fragments arranged by size: 3056 2958 998 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7108 XbaI T'CTAG_A Cuts at: 4444 4444 Size: 7108 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2288 2321 3031 2288 Size: 33 710 6365 Fragments arranged by size: 6365 710 33 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7108 XmaI C'CCGG_G Cuts at: 1870 2173 2625 3129 4433 1870 Size: 303 452 504 1304 4545 Fragments arranged by size: 4545 1304 504 452 303 XmnI GAAnn'nnTTC Cuts at: 2478 5237 2478 Size: 2759 4349 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4916 276 Size: 53 83 186 936 3382 2468 Fragments arranged by size: 3382 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SacII SfoI SrfI SwaI