Restriction map for
pFN21ASDB0057
(Linear) (Six-base) MAPSORT of: KIBB0057.seq Check: 8870 from: 1 to: 2379
KIBB0057.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AccI GT'mk_AC
Cuts at: 0 524 2379
Size: 524 1855
Acc65I G'GTAC_C
Cuts at: 0 1259 2379
Size: 1259 1120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1189 1642 2379
Size: 1189 453 737
Fragments arranged by size:
1189 737 453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1652 2379
Size: 1652 727
AlwNI CAG_nnn'CTG
Cuts at: 0 96 417 1464 1845 1989 2285 2379
Size: 96 321 1047 381 144 296 94
Fragments arranged by size:
1047 381 321 296 144 96 94
ApaI G_GGCC'C
Cuts at: 0 1273 2379
Size: 1273 1106
ApoI r'AATT_y
Cuts at: 0 1144 2173 2379
Size: 1144 1029 206
Fragments arranged by size:
1144 1029 206
AvaI C'yCGr_G
Cuts at: 0 150 340 511 602 804 1106 2075 2379
Size: 150 190 171 91 202 302 969 304
Fragments arranged by size:
969 304 302 202 190 171 150 91
BanI G'GyrC_C
Cuts at: 0 47 1259 1714 1992 2288 2379
Size: 47 1212 455 278 296 91
Fragments arranged by size:
1212 455 296 278 91 47
BanII G_rGCy'C
Cuts at: 0 341 1273 1709 2379
Size: 341 932 436 670
Fragments arranged by size:
932 670 436 341
BbvCI CC'TCA_GC
Cuts at: 0 58 2300 2379
Size: 58 2242 79
Fragments arranged by size:
2242 79 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1633 1667 2379
Size: 1633 34 712
Fragments arranged by size:
1633 712 34
BglI GCCn_nnn'nGGC
Cuts at: 0 25 296 1291 2335 2379
Size: 25 271 995 1044 44
Fragments arranged by size:
1044 995 271 44 25
BglII A'GATC_T
Cuts at: 0 440 2379
Size: 440 1939
Bme1580I G_kGCm'C
Cuts at: 0 301 1273 1717 2379
Size: 301 972 444 662
Fragments arranged by size:
972 662 444 301
BmgBI CAC'GTC
Cuts at: 0 108 2261 2379
Size: 108 2153 118
Fragments arranged by size:
2153 118 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 124 1033 1267 2379
Size: 124 909 234 1112
Fragments arranged by size:
1112 909 234 124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 484 654 1074 2379
Size: 484 170 420 1305
Fragments arranged by size:
1305 484 420 170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 961 1201 1366 1423 1489 1510 1867
Size: 241 720 240 165 57 66 21 357
Cuts at: 1867 2138 2155 2379
Size: 271 17 224
Fragments arranged by size:
720 357 271 241 240 224 165 66
57 21 17
Bpu10I CC'TnA_GC
Cuts at: 0 58 2300 2379
Size: 58 2242 79
Fragments arranged by size:
2242 79 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 1747 1864 1870 2379
Size: 16 1731 117 6 509
Fragments arranged by size:
1731 509 117 16 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 455 2379
Size: 455 1924
BsaHI Gr'CG_yC
Cuts at: 0 182 521 2003 2379
Size: 182 339 1482 376
Fragments arranged by size:
1482 376 339 182
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 454 484 526 556 940 970 1957 1987
Size: 454 30 42 30 384 30 987 30
Cuts at: 1987 2379
Size: 392
Fragments arranged by size:
987 454 392 384 42 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 531 1168 1315 1420 1423 1465 1486
Size: 85 446 637 147 105 3 42 21
Cuts at: 1486 1552 1708 1864 1936 1978 1981 2379
Size: 66 156 156 72 42 3 398
Fragments arranged by size:
637 446 398 156 156 147 105 85
72 66 42 42 21 3 3
BseYI C'CCAG_C
Cuts at: 0 1175 1197 1334 2379
Size: 1175 22 137 1045
Fragments arranged by size:
1175 1045 137 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 2379
Size: 696 1683
BsiHKAI G_wGCw'C
Cuts at: 0 257 377 937 1709 2379
Size: 257 120 560 772 670
Fragments arranged by size:
772 670 560 257 120
BsmI GAATG_Cn'
Cuts at: 0 369 1002 2379
Size: 369 633 1377
Fragments arranged by size:
1377 633 369
Bsp1286I G_dGCh'C
Cuts at: 0 257 301 341 377 937 1273 1709 1717
Size: 257 44 40 36 560 336 436 8
Cuts at: 1717 2379
Size: 662
Fragments arranged by size:
662 560 436 336 257 44 40 36
8
BspHI T'CATG_A
Cuts at: 0 1937 2379
Size: 1937 442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 473 1388 1505 2379
Size: 473 915 117 874
Fragments arranged by size:
915 874 473 117
BsrBI CCG'CTC
Cuts at: 0 398 644 1398 1659 2379
Size: 398 246 754 261 720
Fragments arranged by size:
754 720 398 261 246
BsrDI GCAATG_nn'
Cuts at: 0 2096 2379
Size: 2096 283
BsrFI r'CCGG_y
Cuts at: 0 1943 1999 2379
Size: 1943 56 380
Fragments arranged by size:
1943 380 56
BssSI C'ACGA_G
Cuts at: 0 85 730 2201 2379
Size: 85 645 1471 178
Fragments arranged by size:
1471 645 178 85
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1464 2379
Size: 1464 915
BstEII G'GTnAC_C
Cuts at: 0 123 2379
Size: 123 2256
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2091 2379
Size: 2091 288
BstYI r'GATC_y
Cuts at: 0 92 440 1802 2281 2379
Size: 92 348 1362 479 98
Fragments arranged by size:
1362 479 348 98 92
Bsu36I CC'TnA_GG
Cuts at: 0 860 1265 2379
Size: 860 405 1114
Fragments arranged by size:
1114 860 405
BtgI C'CryG_G
Cuts at: 0 101 1547 2084 2379
Size: 101 1446 537 295
Fragments arranged by size:
1446 537 295 101
BtsI GCAGTG_nn'
Cuts at: 0 837 1132 1978 2048 2379
Size: 837 295 846 70 331
Fragments arranged by size:
846 837 331 295 70
EaeI y'GGCC_r
Cuts at: 0 2 288 696 1532 2029 2379
Size: 2 286 408 836 497 350
Fragments arranged by size:
836 497 408 350 286 2
EarI CTCTTCn'nnn_
Cuts at: 0 446 1180 1213 1292 1618 2379
Size: 446 734 33 79 326 761
Fragments arranged by size:
761 734 446 326 79 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1236 1813 2379
Size: 1236 577 566
Fragments arranged by size:
1236 577 566
EcoICRI GAG'CTC
Cuts at: 0 1707 2379
Size: 1707 672
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 961 1189 1201 1366 1423 1489 1510
Size: 241 720 228 12 165 57 66 21
Cuts at: 1510 1642 1867 2138 2155 2379
Size: 132 225 271 17 224
Fragments arranged by size:
720 271 241 228 225 224 165 132
66 57 21 17 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1732 1849 2379
Size: 1732 117 530
Fragments arranged by size:
1732 530 117
EcoO109I rG'GnC_Cy
Cuts at: 0 27 236 326 534 769 1119 1269 1494
Size: 27 209 90 208 235 350 150 225
Cuts at: 1494 1842 2096 2379
Size: 348 254 283
Fragments arranged by size:
350 348 283 254 235 225 209 208
150 90 27
EcoRI G'AATT_C
Cuts at: 0 1144 2173 2379
Size: 1144 1029 206
Fragments arranged by size:
1144 1029 206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 454 486 526 558 2379
Size: 184 32 238 32 40 32 1821
Fragments arranged by size:
1821 238 184 40 32 32 32
KpnI G_GTAC'C
Cuts at: 0 1263 2379
Size: 1263 1116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1595 2120 2379
Size: 1595 525 259
Fragments arranged by size:
1595 525 259
MscI TGG'CCA
Cuts at: 0 4 290 698 2379
Size: 4 286 408 1681
Fragments arranged by size:
1681 408 286 4
MslI CAynn'nnrTG
Cuts at: 0 106 622 626 667 712 2089 2379
Size: 106 516 4 41 45 1377 290
Fragments arranged by size:
1377 516 290 106 45 41 4
MspA1I CmG'CkG
Cuts at: 0 781 1179 2158 2304 2379
Size: 781 398 979 146 75
Fragments arranged by size:
979 781 398 146 75
NaeI GCC'GGC
Cuts at: 0 2001 2379
Size: 2001 378
NcoI C'CATG_G
Cuts at: 0 101 1547 2084 2379
Size: 101 1446 537 295
Fragments arranged by size:
1446 537 295 101
NgoMIV G'CCGG_C
Cuts at: 0 1999 2379
Size: 1999 380
NspI r_CATG'y
Cuts at: 0 580 2329 2379
Size: 580 1749 50
Fragments arranged by size:
1749 580 50
PasI CC'CwG_GG
Cuts at: 0 897 987 2379
Size: 897 90 1392
Fragments arranged by size:
1392 897 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 1989 2285 2379
Size: 1989 296 94
Fragments arranged by size:
1989 296 94
PfoI T'CCnGG_A
Cuts at: 0 977 1343 1424 1685 1805 1952 2379
Size: 977 366 81 261 120 147 427
Fragments arranged by size:
977 427 366 261 147 120 81
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1868 1900 2379
Size: 1868 32 479
Fragments arranged by size:
1868 479 32
PpuMI rG'GwC_Cy
Cuts at: 0 326 534 1119 1494 1842 2096 2379
Size: 326 208 585 375 348 254 283
Fragments arranged by size:
585 375 348 326 283 254 208
PshAI GACnn'nnGTC
Cuts at: 0 520 2379
Size: 520 1859
PspOMI G'GGCC_C
Cuts at: 0 1269 2379
Size: 1269 1110
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 397 429 2379
Size: 397 32 1950
Fragments arranged by size:
1950 397 32
PstI C_TGCA'G
Cuts at: 0 1385 1733 1787 2043 2379
Size: 1385 348 54 256 336
Fragments arranged by size:
1385 348 336 256 54
PvuII CAG'CTG
Cuts at: 0 1179 2158 2379
Size: 1179 979 221
Fragments arranged by size:
1179 979 221
SacI G_AGCT'C
Cuts at: 0 1709 2379
Size: 1709 670
SanDI GG'GwC_CC
Cuts at: 0 2096 2379
Size: 2096 283
SapI GCTCTTCn'nnn_
Cuts at: 0 1213 2379
Size: 1213 1166
SbfI CC_TGCA'GG
Cuts at: 0 1385 2379
Size: 1385 994
SexAI A'CCwGG_T
Cuts at: 0 416 2379
Size: 416 1963
SfcI C'TryA_G
Cuts at: 0 1381 1729 1783 2039 2379
Size: 1381 348 54 256 340
Fragments arranged by size:
1381 348 340 256 54
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 25 2379
Size: 25 2354
SmaI CCC'GGG
Cuts at: 0 152 604 1108 2379
Size: 152 452 504 1271
Fragments arranged by size:
1271 504 452 152
SmlI C'TyrA_G
Cuts at: 0 31 1762 1879 1885 2379
Size: 31 1731 117 6 494
Fragments arranged by size:
1731 494 117 31 6
StuI AGG'CCT
Cuts at: 0 858 1285 2058 2379
Size: 858 427 773 321
Fragments arranged by size:
858 773 427 321
StyI C'CwwG_G
Cuts at: 0 14 101 473 1124 1355 1547 2084 2379
Size: 14 87 372 651 231 192 537 295
Fragments arranged by size:
651 537 372 295 231 192 87 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 321 400 634 952 1278 2379
Size: 321 79 234 318 326 1101
Fragments arranged by size:
1101 326 321 318 234 79
TatI w'GTAC_w
Cuts at: 0 1538 2379
Size: 1538 841
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 927 959 1957 1989 2379
Size: 927 32 998 32 390
Fragments arranged by size:
998 927 390 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 265 298 1008 2379
Size: 265 33 710 1371
Fragments arranged by size:
1371 710 265 33
XmaI C'CCGG_G
Cuts at: 0 150 602 1106 2379
Size: 150 452 504 1273
Fragments arranged by size:
1273 504 452 150
XmnI GAAnn'nnTTC
Cuts at: 0 455 2379
Size: 455 1924
Enzymes that do cut:
AccI Acc65I AcuI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbvCI BcgI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI Hin4I KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SacI
SanDI SapI SbfI SexAI SfcI SfiI SmaI SmlI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BciVI BclI BfrBI BlpI BmtI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BsrGI
BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PsiI PspXI PvuI RsrII SacII SalI ScaI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI ZraI