(Circular) (Six-base) MAPSORT of: pFN21ASDB0052.seq Check: 9016 from: 1 to: 6592 pFN21ASDB0052 6592 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2985 2985 Size: 6592 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3870 4402 278 Size: 53 83 186 936 2334 532 2468 Fragments arranged by size: 2468 2334 936 532 186 83 53 AccI GT'mk_AC Cuts at: 1937 3935 1937 Size: 1998 4594 Acc65I G'GTAC_C Cuts at: 3913 3913 Size: 6592 AclI AA'CG_TT Cuts at: 1563 4719 5092 1563 Size: 3156 373 3063 Fragments arranged by size: 3156 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2384 2825 3020 3245 4655 6039 6307 1632 Size: 752 441 195 225 1410 1384 268 1917 Fragments arranged by size: 1917 1410 1384 752 441 268 225 195 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6395 AflIII A'CryG_T Cuts at: 1203 5491 6468 1203 Size: 4288 977 1327 Fragments arranged by size: 4288 1327 977 AgeI A'CCGG_T Cuts at: 5423 5423 Size: 6592 AhdI GACnn_n'nnGTC Cuts at: 5321 5321 Size: 6592 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3304 3779 1184 Size: 2120 475 3997 Fragments arranged by size: 3997 2120 475 AlwNI CAG_nnn'CTG Cuts at: 2748 2906 3107 3664 3859 4335 5907 2748 Size: 158 201 557 195 476 1572 3433 Fragments arranged by size: 3433 1572 557 476 201 195 158 ApaLI G'TGCA_C Cuts at: 4649 5805 4649 Size: 1156 5436 ApoI r'AATT_y Cuts at: 1493 2847 3901 4127 4279 6388 1493 Size: 1354 1054 226 152 2109 1697 Fragments arranged by size: 2109 1697 1354 1054 226 152 AseI AT'TA_AT Cuts at: 160 1032 5146 160 Size: 872 4114 1606 Fragments arranged by size: 4114 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 AvaI C'yCGr_G Cuts at: 1870 1943 3790 3917 1870 Size: 73 1847 127 4545 Fragments arranged by size: 4545 1847 127 73 BamHI G'GATC_C Cuts at: 3922 4289 3922 Size: 367 6225 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2315 2973 3691 3913 5368 Size: 325 217 627 528 658 718 222 1455 Cuts at: 5368 618 Size: 1842 Fragments arranged by size: 1842 1455 718 658 627 528 325 222 217 BanII G_rGCy'C Cuts at: 1391 2130 2433 3911 1391 Size: 739 303 1478 4072 Fragments arranged by size: 4072 1478 739 303 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3233 928 Size: 971 1334 4287 Fragments arranged by size: 4287 1334 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2297 2331 4783 4817 1903 Size: 34 360 34 2452 34 3678 Fragments arranged by size: 3678 2452 360 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4484 5694 4484 Size: 1210 5382 BclI T'GATC_A Cuts at: 1548 1548 Size: 6592 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5203 136 Size: 107 122 71 631 605 3531 1525 Fragments arranged by size: 3531 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6587 6587 Size: 6592 BlpI GC'TnA_GC Cuts at: 4009 4009 Size: 6592 Bme1580I G_kGCm'C Cuts at: 1790 2976 3694 4653 5809 1790 Size: 1186 718 959 1156 2573 Fragments arranged by size: 2573 1186 1156 959 718 BmgBI CAC'GTC Cuts at: 1318 3559 1318 Size: 2241 4351 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2287 3057 3607 Size: 416 53 83 399 24 870 770 550 Cuts at: 3607 4323 5281 442 Size: 716 958 1753 Fragments arranged by size: 1753 958 870 770 716 550 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6592 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 6592 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2154 2363 2426 3063 3486 3670 3717 Size: 903 12 209 63 637 423 184 47 Cuts at: 3717 5252 1239 Size: 1535 2579 Fragments arranged by size: 2579 1535 903 637 423 209 184 63 47 12 Bpu10I CC'TnA_GC Cuts at: 3276 3276 Size: 6592 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2241 4717 5582 5880 2241 Size: 2476 865 298 2953 Fragments arranged by size: 2953 2476 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2267 2436 2747 5255 882 Size: 617 768 169 311 2508 2219 Fragments arranged by size: 2508 2219 768 617 311 169 BsaAI yAC'GTr Cuts at: 493 1204 3889 4383 493 Size: 711 2685 494 2702 Fragments arranged by size: 2702 2685 711 494 BsaBI GATnn'nnATC Cuts at: 3392 6586 3392 Size: 3194 3398 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3786 3867 4399 4781 Size: 53 83 186 936 2253 81 532 382 Cuts at: 4781 5416 275 Size: 635 1451 Fragments arranged by size: 2253 1451 936 635 532 382 186 83 81 53 BsaWI w'CCGG_w Cuts at: 3777 5025 5423 5697 5844 6255 3777 Size: 1248 398 274 147 411 4114 Fragments arranged by size: 4114 1248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6367 Fragments arranged by size: 6367 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2423 3535 1186 Size: 1237 1112 4243 Fragments arranged by size: 4243 1237 1112 BseYI C'CCAG_C Cuts at: 2218 2446 3751 5795 6291 2218 Size: 228 1305 2044 496 2519 Fragments arranged by size: 2519 2044 1305 496 228 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2305 2770 2867 3785 1163 Size: 573 569 465 97 918 3970 Fragments arranged by size: 3970 918 573 569 465 97 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4042 4803 5831 1540 Size: 275 186 2041 761 1028 2301 Fragments arranged by size: 2301 2041 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2130 3039 4653 4738 5809 2130 Size: 909 1614 85 1071 2913 Fragments arranged by size: 2913 1614 1071 909 85 BsmI GAATG_Cn' Cuts at: 3281 4103 4196 3281 Size: 822 93 5677 Fragments arranged by size: 5677 822 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3793 3793 Size: 6592 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2433 2976 3039 3694 3911 4653 Size: 399 340 303 543 63 655 217 742 Cuts at: 4653 4738 5809 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 655 543 399 340 303 217 85 63 BspEI T'CCGG_A Cuts at: 3777 6255 3777 Size: 2478 4114 BspHI T'CATG_A Cuts at: 3070 4481 6211 3070 Size: 1411 1730 3451 Fragments arranged by size: 3451 1730 1411 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2441 2668 2985 3947 844 Size: 1597 227 317 962 3489 Fragments arranged by size: 3489 1597 962 317 227 BsrBI CCG'CTC Cuts at: 1394 1823 3030 4479 1394 Size: 429 1207 1449 3507 Fragments arranged by size: 3507 1449 1207 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3579 5087 5261 65 Size: 1183 2331 1508 174 1396 Fragments arranged by size: 2331 1508 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2324 5236 5423 1700 Size: 110 514 2912 187 2869 Fragments arranged by size: 2912 2869 514 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6592 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6592 BssSI C'ACGA_G Cuts at: 4652 5664 4652 Size: 1012 5580 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6592 BstBI TT'CG_AA Cuts at: 2007 5396 2007 Size: 3389 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6592 BstXI CCAn_nnnn'nTGG Cuts at: 2146 2241 2709 2146 Size: 95 468 6029 Fragments arranged by size: 6029 468 95 BstYI r'GATC_y Cuts at: 1972 2875 3234 3922 4289 4674 4691 6143 6229 Size: 903 359 688 367 385 17 1452 86 Cuts at: 6229 6241 6587 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 903 688 385 367 359 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2042 3665 2042 Size: 1623 4969 BtgI C'CryG_G Cuts at: 513 1065 2676 3873 513 Size: 552 1611 1197 3232 Fragments arranged by size: 3232 1611 1197 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2878 3532 487 Size: 683 56 1652 654 3547 Fragments arranged by size: 3547 1652 683 654 56 BtsI GCAGTG_nn' Cuts at: 4108 4902 4922 4108 Size: 794 20 5778 Fragments arranged by size: 5778 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6592 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6557 DraI TTT'AAA Cuts at: 3896 3896 Size: 6592 DraIII CAC_nnn'GTG Cuts at: 3331 3331 Size: 6592 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3575 5599 809 Size: 507 2259 2024 1802 Fragments arranged by size: 2259 2024 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3333 4039 4928 Size: 54 1425 50 275 18 1503 706 889 Cuts at: 4928 6557 8 Size: 1629 43 Fragments arranged by size: 1629 1503 1425 889 706 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4039 1537 Size: 275 2227 4090 Fragments arranged by size: 4090 2227 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2434 3078 3346 4522 1353 Size: 1081 644 268 1176 3423 Fragments arranged by size: 3423 1176 1081 644 268 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5179 5553 5699 387 Size: 1064 138 3590 374 146 1280 Fragments arranged by size: 3590 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2128 2128 Size: 6592 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2154 2363 2384 2426 2825 3020 Size: 393 510 12 209 21 42 399 195 Cuts at: 3020 3063 3245 3486 3670 3717 4655 5252 6039 Size: 43 182 241 184 47 938 597 787 Cuts at: 6039 6307 1239 Size: 268 1524 Fragments arranged by size: 1524 938 787 597 510 399 393 268 241 209 195 184 182 47 43 42 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 2408 2408 Size: 6592 EcoO109I rG'GnC_Cy Cuts at: 2046 2160 3301 3718 2046 Size: 114 1141 417 4920 Fragments arranged by size: 4920 1141 417 114 EcoRI G'AATT_C Cuts at: 2847 3901 4279 2847 Size: 1054 378 5160 Fragments arranged by size: 5160 1054 378 FspI TGC'GCA Cuts at: 3622 5098 3622 Size: 1476 5116 HaeII r_GCGC'y Cuts at: 1678 5739 1678 Size: 4061 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2272 2304 5233 5265 5307 Size: 32 135 32 700 32 2929 32 42 Cuts at: 5307 5339 1373 Size: 32 2626 Fragments arranged by size: 2929 2626 700 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2197 3163 3409 3936 4182 669 Size: 988 281 259 966 246 527 246 3079 Fragments arranged by size: 3079 988 966 527 281 259 246 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6592 HpaI GTT'AAC Cuts at: 4182 4182 Size: 6592 KpnI G_GTAC'C Cuts at: 3917 3917 Size: 6592 MfeI C'AATT_G Cuts at: 4191 4191 Size: 6592 MluI A'CGCG_T Cuts at: 6468 6468 Size: 6592 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2155 3658 5706 5890 1305 Size: 850 1503 2048 184 2007 Fragments arranged by size: 2048 2007 1503 850 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3335 10 Size: 54 1425 343 1503 3267 Fragments arranged by size: 3267 1503 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2792 3286 3540 3761 3809 4550 4909 Size: 648 1626 494 254 221 48 741 359 Cuts at: 4909 5068 518 Size: 159 2042 Fragments arranged by size: 2042 1626 741 648 494 359 254 221 159 48 MspA1I CmG'CkG Cuts at: 1606 2362 2722 4008 4685 5833 6078 1606 Size: 756 360 1286 677 1148 245 2120 Fragments arranged by size: 2120 1286 1148 756 677 360 245 NcoI C'CATG_G Cuts at: 513 1065 2676 513 Size: 552 1611 4429 Fragments arranged by size: 4429 1611 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6592 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6592 NotI GC'GGCC_GC Cuts at: 4039 4039 Size: 6592 NspI r_CATG'y Cuts at: 1132 1229 3950 5495 1132 Size: 97 2721 1545 2229 Fragments arranged by size: 2721 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2690 2954 1348 Size: 1342 264 4986 Fragments arranged by size: 4986 1342 264 PciI A'CATG_T Cuts at: 5491 5491 Size: 6592 PflMI CCAn_nnn'nTGG Cuts at: 1108 3331 3859 1108 Size: 2223 528 3841 Fragments arranged by size: 3841 2223 528 PfoI T'CCnGG_A Cuts at: 3448 3448 Size: 6592 PmeI GTTT'AAAC Cuts at: 3896 3896 Size: 6592 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4661 4693 6174 6206 4661 Size: 32 1481 32 5047 Fragments arranged by size: 5047 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2046 2160 3301 2046 Size: 114 1141 5337 Fragments arranged by size: 5337 1141 114 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6592 PsiI TTA'TAA Cuts at: 4162 4162 Size: 6592 PstI C_TGCA'G Cuts at: 830 2396 3132 3944 830 Size: 1566 736 812 3478 Fragments arranged by size: 3478 1566 812 736 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6592 PvuII CAG'CTG Cuts at: 2362 2362 Size: 6592 SacI G_AGCT'C Cuts at: 2130 2130 Size: 6592 SalI G'TCGA_C Cuts at: 1936 3934 1936 Size: 1998 4594 SanDI GG'GwC_CC Cuts at: 3301 3301 Size: 6592 SapI GCTCTTCn'nnn_ Cuts at: 3346 3346 Size: 6592 SbfI CC_TGCA'GG Cuts at: 3132 3944 3132 Size: 812 5780 ScaI AGT'ACT Cuts at: 2351 4840 6552 2351 Size: 2489 1712 2391 Fragments arranged by size: 2489 2391 1712 SexAI A'CCwGG_T Cuts at: 2824 3327 2824 Size: 503 6089 SfcI C'TryA_G Cuts at: 826 1045 2392 2887 3128 3940 5075 5756 5947 Size: 219 1347 495 241 812 1135 681 191 Cuts at: 5947 826 Size: 1471 Fragments arranged by size: 1471 1347 1135 812 681 495 241 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6592 SmaI CCC'GGG Cuts at: 1872 3919 1872 Size: 2047 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2256 4696 5597 5859 820 Size: 197 926 313 2440 901 262 1553 Fragments arranged by size: 2440 1553 926 901 313 262 197 SnaBI TAC'GTA Cuts at: 493 3889 4383 493 Size: 3396 494 2702 Fragments arranged by size: 3396 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6592 SphI G_CATG'C Cuts at: 1132 3950 1132 Size: 2818 3774 SspI AAT'ATT Cuts at: 5 52 4516 5 Size: 47 4464 2081 Fragments arranged by size: 4464 2081 47 StuI AGG'CCT Cuts at: 1517 2414 3005 3134 3707 1517 Size: 897 591 129 573 4402 Fragments arranged by size: 4402 897 591 573 129 StyI C'CwwG_G Cuts at: 513 1065 2415 2547 2676 3135 3253 4031 513 Size: 552 1350 132 129 459 118 778 3074 Fragments arranged by size: 3074 1350 778 552 459 132 129 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3718 3851 4619 4636 4789 4974 5419 Size: 1255 2138 133 768 17 153 185 445 Cuts at: 5419 325 Size: 1498 Fragments arranged by size: 2138 1498 1255 768 445 185 153 133 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2349 3636 4838 Size: 274 80 33 51 1443 372 1287 1202 Cuts at: 4838 6550 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1287 1202 372 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2030 2062 3615 3647 6552 6584 2030 Size: 32 1553 32 2905 32 2038 Fragments arranged by size: 2905 2038 1553 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6592 XbaI T'CTAG_A Cuts at: 3928 3928 Size: 6592 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2198 2554 2198 Size: 356 6236 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6592 XmaI C'CCGG_G Cuts at: 1870 3917 1870 Size: 2047 4545 XmnI GAAnn'nnTTC Cuts at: 4721 4721 Size: 6592 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3868 4400 276 Size: 53 83 186 936 2334 532 2468 Fragments arranged by size: 2468 2334 936 532 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI