(Circular) (Six-base) MAPSORT of: pFN21ASDB0042.seq Check: 3235 from: 1 to: 6742 pFN21ASDB0042 6742 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4552 278 Size: 53 83 186 936 3016 2468 Fragments arranged by size: 3016 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3771 4085 1937 Size: 1834 314 4594 Fragments arranged by size: 4594 1834 314 Acc65I G'GTAC_C Cuts at: 2315 4063 2315 Size: 1748 4994 AclI AA'CG_TT Cuts at: 1563 4869 5242 1563 Size: 3306 373 3063 Fragments arranged by size: 3306 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2811 3134 3645 4805 6189 6457 1632 Size: 1179 323 511 1160 1384 268 1917 Fragments arranged by size: 1917 1384 1179 1160 511 323 268 AfeI AGC'GCT Cuts at: 3197 3197 Size: 6742 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6545 AflIII A'CryG_T Cuts at: 1203 5641 6618 1203 Size: 4438 977 1327 Fragments arranged by size: 4438 1327 977 AgeI A'CCGG_T Cuts at: 5573 5573 Size: 6742 AhdI GACnn_n'nnGTC Cuts at: 5471 5471 Size: 6742 AleI CACnn'nnGTG Cuts at: 1166 2196 1166 Size: 1030 5712 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6742 AlwNI CAG_nnn'CTG Cuts at: 3139 4027 4485 6057 3139 Size: 888 458 1572 3824 Fragments arranged by size: 3824 1572 888 458 ApaLI G'TGCA_C Cuts at: 4799 5955 4799 Size: 1156 5586 ApoI r'AATT_y Cuts at: 1493 4051 4277 4429 6538 1493 Size: 2558 226 152 2109 1697 Fragments arranged by size: 2558 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5296 160 Size: 872 4264 1606 Fragments arranged by size: 4264 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6742 AvaI C'yCGr_G Cuts at: 1870 1943 4067 1870 Size: 73 2124 4545 Fragments arranged by size: 4545 2124 73 BamHI G'GATC_C Cuts at: 4072 4439 4072 Size: 367 6375 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2217 2315 4063 5518 618 Size: 325 217 627 430 98 1748 1455 1842 Fragments arranged by size: 1842 1748 1455 627 430 325 217 98 BanII G_rGCy'C Cuts at: 1391 2559 3606 3840 4061 1391 Size: 1168 1047 234 221 4072 Fragments arranged by size: 4072 1168 1047 234 221 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2961 3221 3743 928 Size: 971 1062 260 522 3927 Fragments arranged by size: 3927 1062 971 522 260 BbvCI CC'TCA_GC Cuts at: 3305 3305 Size: 6742 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3353 3387 3478 3512 4933 4967 1903 Size: 34 1416 34 91 34 1421 34 3678 Fragments arranged by size: 3678 1421 1416 91 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4634 5844 3674 Size: 960 1210 4572 Fragments arranged by size: 4572 1210 960 BclI T'GATC_A Cuts at: 1548 1548 Size: 6742 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5353 136 Size: 107 122 71 631 605 3681 1525 Fragments arranged by size: 3681 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2851 3793 6737 2851 Size: 942 2944 2856 Fragments arranged by size: 2944 2856 942 BlpI GC'TnA_GC Cuts at: 4159 4159 Size: 6742 Bme1580I G_kGCm'C Cuts at: 1790 2215 4803 5959 1790 Size: 425 2588 1156 2573 Fragments arranged by size: 2588 2573 1156 425 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6742 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2376 2553 3897 Size: 416 53 83 399 24 959 177 1344 Cuts at: 3897 3939 4473 5431 442 Size: 42 534 958 1753 Fragments arranged by size: 1753 1344 959 958 534 416 399 177 83 53 42 24 BmtI G_CTAG'C Cuts at: 1056 2996 1056 Size: 1940 4802 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2688 2688 Size: 6742 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2341 2644 2772 3590 5402 1239 Size: 935 167 303 128 818 1812 2579 Fragments arranged by size: 2579 1812 935 818 303 167 128 Bpu10I CC'TnA_GC Cuts at: 3305 3305 Size: 6742 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2712 4867 5732 6030 2712 Size: 2155 865 298 3424 Fragments arranged by size: 3424 2155 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5405 882 Size: 617 3906 2219 Fragments arranged by size: 3906 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4039 4533 493 Size: 711 2835 494 2702 Fragments arranged by size: 2835 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3050 6736 3050 Size: 3686 3056 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4549 4931 5566 275 Size: 53 83 186 936 3016 382 635 1451 Fragments arranged by size: 3016 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2106 3570 5175 5573 5847 5994 6405 2106 Size: 1464 1605 398 274 147 411 2443 Fragments arranged by size: 2443 1605 1464 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2472 2502 1178 Size: 30 165 30 1069 30 5418 Fragments arranged by size: 5418 1069 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 3010 1186 Size: 886 938 4918 Fragments arranged by size: 4918 938 886 BseYI C'CCAG_C Cuts at: 3541 3874 4027 5945 6441 3541 Size: 333 153 1918 496 3842 Fragments arranged by size: 3842 1918 496 333 153 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6169 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3036 4192 4953 5981 1540 Size: 275 186 1035 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1035 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3606 4803 4888 5959 3606 Size: 1197 85 1071 4389 Fragments arranged by size: 4389 1197 1071 85 BsmI GAATG_Cn' Cuts at: 4253 4346 4253 Size: 93 6649 BsmBI CGTCTCn'nnnn_ Cuts at: 3293 3293 Size: 6742 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2215 2559 3606 3840 4061 4803 4888 Size: 399 425 344 1047 234 221 742 85 Cuts at: 4888 5959 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1047 742 425 399 344 234 221 85 BspEI T'CCGG_A Cuts at: 2106 6405 2106 Size: 4299 2443 BspHI T'CATG_A Cuts at: 4631 6361 4631 Size: 1730 5012 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2461 4097 844 Size: 1617 1636 3489 Fragments arranged by size: 3489 1636 1617 BsrBI CCG'CTC Cuts at: 1394 1823 2545 4629 1394 Size: 429 722 2084 3507 Fragments arranged by size: 3507 2084 722 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2317 3448 3856 5237 5411 65 Size: 1183 1069 1131 408 1381 174 1396 Fragments arranged by size: 1396 1381 1183 1131 1069 408 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5386 5573 1700 Size: 110 3576 187 2869 Fragments arranged by size: 3576 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6742 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6742 BssSI C'ACGA_G Cuts at: 4802 5814 4802 Size: 1012 5730 BstAPI GCAn_nnn'nTGC Cuts at: 794 2334 3958 794 Size: 1540 1624 3578 Fragments arranged by size: 3578 1624 1540 BstBI TT'CG_AA Cuts at: 2007 5546 2007 Size: 3539 3203 BstEII G'GTnAC_C Cuts at: 1184 3566 1184 Size: 2382 4360 BstXI CCAn_nnnn'nTGG Cuts at: 3978 3978 Size: 6742 BstYI r'GATC_y Cuts at: 1972 2851 3315 3408 3793 4072 4439 4824 4841 Size: 879 464 93 385 279 367 385 17 Cuts at: 4841 6293 6379 6391 6737 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 879 464 385 385 367 346 279 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2819 2819 Size: 6742 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6190 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2966 487 Size: 683 56 1740 4263 Fragments arranged by size: 4263 1740 683 56 BtsI GCAGTG_nn' Cuts at: 2463 4258 5052 5072 2463 Size: 1795 794 20 4133 Fragments arranged by size: 4133 1795 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6742 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6707 DraI TTT'AAA Cuts at: 4046 4046 Size: 6742 DraIII CAC_nnn'GTG Cuts at: 2281 2971 2281 Size: 690 6052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3671 5749 809 Size: 507 2355 2078 1802 Fragments arranged by size: 2355 2078 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2519 3843 4014 Size: 54 1425 50 275 18 689 1324 171 Cuts at: 4014 4189 5078 6707 8 Size: 175 889 1629 43 Fragments arranged by size: 1629 1425 1324 889 689 275 175 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4189 1537 Size: 275 2377 4090 Fragments arranged by size: 4090 2377 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2931 4672 1353 Size: 741 837 1741 3423 Fragments arranged by size: 3423 1741 837 741 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5329 5703 5849 387 Size: 1064 138 3740 374 146 1280 Fragments arranged by size: 3740 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3604 3604 Size: 6742 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2341 2644 2772 2811 3134 3590 Size: 393 542 167 303 128 39 323 456 Cuts at: 3590 3645 4805 5402 6189 6457 1239 Size: 55 1160 597 787 268 1524 Fragments arranged by size: 1524 1160 787 597 542 456 393 323 303 268 167 128 55 39 EcoNI CCTnn'n_nnAGG Cuts at: 3398 3561 3398 Size: 163 6579 EcoO109I rG'GnC_Cy Cuts at: 3342 3342 Size: 6742 EcoRI G'AATT_C Cuts at: 4051 4429 4051 Size: 378 6364 FspI TGC'GCA Cuts at: 5248 5248 Size: 6742 HaeII r_GCGC'y Cuts at: 1678 3199 5889 1678 Size: 1521 2690 2531 Fragments arranged by size: 2690 2531 1521 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2472 2504 2608 2640 2642 Size: 32 135 32 900 32 104 32 2 Cuts at: 2642 2674 2701 2733 3730 3762 5383 5415 5457 Size: 32 27 32 997 32 1621 32 42 Cuts at: 5457 5489 1373 Size: 32 2626 Fragments arranged by size: 2626 1621 997 900 135 104 42 32 32 32 32 32 32 32 32 32 27 2 HincII GTy'rAC Cuts at: 669 1657 1938 4086 4332 669 Size: 988 281 2148 246 3079 Fragments arranged by size: 3079 2148 988 281 246 HindIII A'AGCT_T Cuts at: 748 3428 748 Size: 2680 4062 HpaI GTT'AAC Cuts at: 4332 4332 Size: 6742 KpnI G_GTAC'C Cuts at: 2319 4067 2319 Size: 1748 4994 MfeI C'AATT_G Cuts at: 4341 4341 Size: 6742 MluI A'CGCG_T Cuts at: 6618 6618 Size: 6742 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2007 3541 5856 6040 1305 Size: 702 1534 2315 184 2007 Fragments arranged by size: 2315 2007 1534 702 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2521 3845 4016 10 Size: 54 1425 343 689 1324 171 2736 Fragments arranged by size: 2736 1425 1324 689 343 171 54 MslI CAynn'nnrTG Cuts at: 518 1166 2196 2880 3086 4700 5059 5218 518 Size: 648 1030 684 206 1614 359 159 2042 Fragments arranged by size: 2042 1614 1030 684 648 359 206 159 MspA1I CmG'CkG Cuts at: 1606 2642 3169 4158 4835 5983 6228 1606 Size: 1036 527 989 677 1148 245 2120 Fragments arranged by size: 2120 1148 1036 989 677 527 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6190 NdeI CA'TA_TG Cuts at: 387 3208 3804 387 Size: 2821 596 3325 Fragments arranged by size: 3325 2821 596 NheI G'CTAG_C Cuts at: 1052 2992 1052 Size: 1940 4802 NotI GC'GGCC_GC Cuts at: 4189 4189 Size: 6742 NspI r_CATG'y Cuts at: 1132 1229 3010 4100 5645 1132 Size: 97 1781 1090 1545 2229 Fragments arranged by size: 2229 1781 1545 1090 97 PasI CC'CwG_GG Cuts at: 1348 3971 1348 Size: 2623 4119 PciI A'CATG_T Cuts at: 5641 5641 Size: 6742 PflMI CCAn_nnn'nTGG Cuts at: 1108 3139 3169 1108 Size: 2031 30 4681 Fragments arranged by size: 4681 2031 30 PfoI T'CCnGG_A Cuts at: 2660 3422 2660 Size: 762 5980 PmeI GTTT'AAAC Cuts at: 4046 4046 Size: 6742 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4811 4843 6324 6356 4811 Size: 32 1481 32 5197 Fragments arranged by size: 5197 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3342 3342 Size: 6742 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6742 PsiI TTA'TAA Cuts at: 4312 4312 Size: 6742 PstI C_TGCA'G Cuts at: 830 2458 3351 3999 4094 830 Size: 1628 893 648 95 3478 Fragments arranged by size: 3478 1628 893 648 95 PvuI CG_AT'CG Cuts at: 2001 3036 2001 Size: 1035 5707 PvuII CAG'CTG Cuts at: 2642 3169 2642 Size: 527 6215 SacI G_AGCT'C Cuts at: 3606 3606 Size: 6742 SalI G'TCGA_C Cuts at: 1936 4084 1936 Size: 2148 4594 SbfI CC_TGCA'GG Cuts at: 4094 4094 Size: 6742 ScaI AGT'ACT Cuts at: 4990 6702 4990 Size: 1712 5030 SexAI A'CCwGG_T Cuts at: 2277 2277 Size: 6742 SfcI C'TryA_G Cuts at: 826 1045 2087 2194 2454 3055 3347 3797 3995 Size: 219 1042 107 260 601 292 450 198 Cuts at: 3995 4090 5225 5906 6097 826 Size: 95 1135 681 191 1471 Fragments arranged by size: 1471 1135 1042 681 601 450 292 260 219 198 191 107 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6742 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6742 SmaI CCC'GGG Cuts at: 1872 4069 1872 Size: 2197 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2727 4846 5747 6009 820 Size: 197 926 784 2119 901 262 1553 Fragments arranged by size: 2119 1553 926 901 784 262 197 SnaBI TAC'GTA Cuts at: 493 4039 4533 493 Size: 3546 494 2702 Fragments arranged by size: 3546 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6742 SphI G_CATG'C Cuts at: 1132 3010 4100 1132 Size: 1878 1090 3774 Fragments arranged by size: 3774 1878 1090 SspI AAT'ATT Cuts at: 5 52 4666 5 Size: 47 4614 2081 Fragments arranged by size: 4614 2081 47 StuI AGG'CCT Cuts at: 1517 2824 2919 1517 Size: 1307 95 5340 Fragments arranged by size: 5340 1307 95 StyI C'CwwG_G Cuts at: 513 1065 2340 2889 3244 3394 4181 513 Size: 552 1275 549 355 150 787 3074 Fragments arranged by size: 3074 1275 787 552 549 355 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2498 3866 4769 4786 4939 5124 5569 Size: 1255 918 1368 903 17 153 185 445 Cuts at: 5569 325 Size: 1498 Fragments arranged by size: 1498 1368 1255 918 903 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 4988 6700 Size: 274 80 33 51 1443 855 2156 1712 Cuts at: 6700 96 Size: 138 Fragments arranged by size: 2156 1712 1443 855 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3268 3300 6702 6734 3268 Size: 32 3402 32 3276 Fragments arranged by size: 3402 3276 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6742 XbaI T'CTAG_A Cuts at: 4078 4078 Size: 6742 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4024 4024 Size: 6742 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6742 XmaI C'CCGG_G Cuts at: 1870 4067 1870 Size: 2197 4545 XmnI GAAnn'nnTTC Cuts at: 4871 4871 Size: 6742 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4550 276 Size: 53 83 186 936 3016 2468 Fragments arranged by size: 3016 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI