Restriction map for
pFN21ASDB0040
(Circular) (Six-base) MAPSORT of: pFN21ASDB0040.seq Check: 9579 from: 1 to: 6589
pFN21ASDB0040 6589 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2017 2670 3290 3310 3564 2017
Size: 653 620 20 254 5042
Fragments arranged by size:
5042 653 620 254 20
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2822 4399 278
Size: 53 83 186 936 1286 1577 2468
Fragments arranged by size:
2468 1577 1286 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2562 3932 1937
Size: 625 1370 4594
Fragments arranged by size:
4594 1370 625
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2435 2566 4652 6036 6304 1632
Size: 803 131 2086 1384 268 1917
Fragments arranged by size:
2086 1917 1384 803 268 131
AfeI AGC'GCT
Cuts at: 2204 2204
Size: 6589
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6392
AflIII A'CryG_T
Cuts at: 1203 2505 5488 6465 1203
Size: 1302 2983 977 1327
Fragments arranged by size:
2983 1327 1302 977
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 2394 2747 5318 2394
Size: 353 2571 3665
Fragments arranged by size:
3665 2571 353
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6589
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2057 1184
Size: 873 5716
AlwNI CAG_nnn'CTG
Cuts at: 2518 4332 5904 2518
Size: 1814 1572 3203
Fragments arranged by size:
3203 1814 1572
ApaLI G'TGCA_C
Cuts at: 4646 5802 4646
Size: 1156 5433
ApoI r'AATT_y
Cuts at: 1493 3898 4124 4276 6385 1493
Size: 2405 226 152 2109 1697
Fragments arranged by size:
2405 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5143 160
Size: 872 4111 1606
Fragments arranged by size:
4111 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 2219 3218 3914 1870
Size: 73 276 999 696 4545
Fragments arranged by size:
4545 999 696 276 73
AvrII C'CTAG_G
Cuts at: 2254 2254
Size: 6589
BamHI G'GATC_C
Cuts at: 3919 4286 3919
Size: 367 6222
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2360 2691 3168 3910 5365
Size: 325 217 627 573 331 477 742 1455
Cuts at: 5365 618
Size: 1842
Fragments arranged by size:
1842 1455 742 627 573 477 331 325
217
BanII G_rGCy'C
Cuts at: 1391 2120 2328 2358 2936 3006 3908 1391
Size: 729 208 30 578 70 902 4072
Fragments arranged by size:
4072 902 729 578 208 70 30
BbeI G_GCGC'C
Cuts at: 2364 3172 2364
Size: 808 5781
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2428 3076 3442 928
Size: 971 529 648 366 4075
Fragments arranged by size:
4075 971 648 529 366
BbvCI CC'TCA_GC
Cuts at: 2447 2447
Size: 6589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4780 4814 1903
Size: 34 2843 34 3678
Fragments arranged by size:
3678 2843 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4481 5691 4481
Size: 1210 5379
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2805 3583 5200
Size: 107 122 71 631 605 1133 778 1617
Cuts at: 5200 136
Size: 1525
Fragments arranged by size:
1617 1525 1133 778 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2757 6584 2757
Size: 3827 2762
BlpI GC'TnA_GC
Cuts at: 4006 4006
Size: 6589
Bme1580I G_kGCm'C
Cuts at: 1790 2859 3819 4650 5806 1790
Size: 1069 960 831 1156 2573
Fragments arranged by size:
2573 1156 1069 960 831
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6589
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4320 5278 442
Size: 416 53 83 399 24 2903 958 1753
Fragments arranged by size:
2903 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2342 2558 2621 2636 3092 3125 3488
Size: 1094 9 216 63 15 456 33 363
Cuts at: 3488 3787 5249 1239
Size: 299 1462 2579
Fragments arranged by size:
2579 1462 1094 456 363 299 216 63
33 15 9
Bpu10I CC'TnA_GC
Cuts at: 2447 2447
Size: 6589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2729 4714 5579 5877 2513
Size: 216 1985 865 298 3225
Fragments arranged by size:
3225 1985 865 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2402 3424 5252 882
Size: 617 903 1022 1828 2219
Fragments arranged by size:
2219 1828 1022 903 617
BsaAI yAC'GTr
Cuts at: 493 1204 3207 3504 3886 4380 493
Size: 711 2003 297 382 494 2702
Fragments arranged by size:
2702 2003 711 494 382 297
BsaBI GATnn'nnATC
Cuts at: 2489 6583 2489
Size: 4094 2495
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2361 2433 2819 3169
Size: 53 83 186 936 828 72 386 350
Cuts at: 3169 3732 4396 4778 5413 275
Size: 563 664 382 635 1451
Fragments arranged by size:
1451 936 828 664 635 563 386 382
350 186 83 72 53
BsaWI w'CCGG_w
Cuts at: 2385 5022 5420 5694 5841 6252 2385
Size: 2637 398 274 147 411 2722
Fragments arranged by size:
2722 2637 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2618 2648 1178
Size: 30 165 30 1215 30 5119
Fragments arranged by size:
5119 1215 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2074 2330 1186
Size: 888 256 5445
Fragments arranged by size:
5445 888 256
BseYI C'CCAG_C
Cuts at: 2069 2113 2392 3315 5792 6288 2069
Size: 44 279 923 2477 496 2370
Fragments arranged by size:
2477 2370 923 496 279 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2648 3368 1163
Size: 573 912 720 4384
Fragments arranged by size:
4384 912 720 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4039 4800 5828 1540
Size: 275 186 2038 761 1028 2301
Fragments arranged by size:
2301 2038 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2168 2328 3042 4650 4735 5806 2168
Size: 160 714 1608 85 1071 2951
Fragments arranged by size:
2951 1608 1071 714 160 85
BsmI GAATG_Cn'
Cuts at: 4100 4193 4100
Size: 93 6496
BsmBI CGTCTCn'nnnn_
Cuts at: 2231 2231
Size: 6589
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2168 2328 2358 2859 2936 3006
Size: 399 330 48 160 30 501 77 70
Cuts at: 3006 3042 3819 3908 4650 4735 5806 1391
Size: 36 777 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 777 742 501 399 330 160
89 85 77 70 48 36 30
BspEI T'CCGG_A
Cuts at: 2385 6252 2385
Size: 3867 2722
BspHI T'CATG_A
Cuts at: 4478 6208 4478
Size: 1730 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2017 2670 3039 3290 3310 3564 3944 844
Size: 1173 653 369 251 20 254 380 3489
Fragments arranged by size:
3489 1173 653 380 369 254 251 20
BsrBI CCG'CTC
Cuts at: 1394 1823 4476 1394
Size: 429 2653 3507
Fragments arranged by size:
3507 2653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3439 5084 5258 65
Size: 1183 2191 1645 174 1396
Fragments arranged by size:
2191 1645 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3192 5233 5420 1700
Size: 110 1382 2041 187 2869
Fragments arranged by size:
2869 2041 1382 187 110
BsrGI T'GTAC_A
Cuts at: 96 2520 96
Size: 2424 4165
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6589
BssSI C'ACGA_G
Cuts at: 4649 5661 4649
Size: 1012 5577
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2950 794
Size: 2156 4433
BstBI TT'CG_AA
Cuts at: 2007 5393 2007
Size: 3386 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6589
BstXI CCAn_nnnn'nTGG
Cuts at: 3709 3709
Size: 6589
BstYI r'GATC_y
Cuts at: 1972 2757 2967 3919 4286 4671 4688 6140 6226
Size: 785 210 952 367 385 17 1452 86
Cuts at: 6226 6238 6584 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 952 785 385 367 346 210
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2681 2681
Size: 6589
BtgI C'CryG_G
Cuts at: 513 1065 2139 2349 2994 3016 3708 513
Size: 552 1074 210 645 22 692 3394
Fragments arranged by size:
3394 1074 692 645 552 210 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3499 3682 487
Size: 683 56 2273 183 3394
Fragments arranged by size:
3394 2273 683 183 56
BtsI GCAGTG_nn'
Cuts at: 2836 4105 4899 4919 2836
Size: 1269 794 20 4506
Fragments arranged by size:
4506 1269 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6554
DraI TTT'AAA
Cuts at: 3893 3893
Size: 6589
DraIII CAC_nnn'GTG
Cuts at: 3094 3346 3094
Size: 252 6337
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2824 5596 809
Size: 507 1508 2772 1802
Fragments arranged by size:
2772 1802 1508 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2865 2991
Size: 54 1425 50 275 18 306 729 126
Cuts at: 2991 3519 4036 4925 6554 8
Size: 528 517 889 1629 43
Fragments arranged by size:
1629 1425 889 729 528 517 306 275
126 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4036 1537
Size: 275 2224 4090
Fragments arranged by size:
4090 2224 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2381 3746 4519 1353
Size: 1028 1365 773 3423
Fragments arranged by size:
3423 1365 1028 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2158 2861 3835 5176 5550 5696
Size: 1064 138 569 703 974 1341 374 146
Cuts at: 5696 387
Size: 1280
Fragments arranged by size:
1341 1280 1064 974 703 569 374 146
138
EcoICRI GAG'CTC
Cuts at: 2326 2326
Size: 6589
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2342 2435 2558 2566 2621 2636
Size: 393 701 9 93 123 8 55 15
Cuts at: 2636 3092 3125 3488 3787 4652 5249 6036 6304
Size: 456 33 363 299 865 597 787 268
Cuts at: 6304 1239
Size: 1524
Fragments arranged by size:
1524 865 787 701 597 456 393 363
299 268 123 93 55 33 15 9
8
EcoNI CCTnn'n_nnAGG
Cuts at: 2728 2728
Size: 6589
EcoO109I rG'GnC_Cy
Cuts at: 2515 2515
Size: 6589
EcoRI G'AATT_C
Cuts at: 3898 4276 3898
Size: 378 6211
FspI TGC'GCA
Cuts at: 3566 5095 3566
Size: 1529 5060
HaeII r_GCGC'y
Cuts at: 1678 2206 2349 2364 2654 3172 5736 1678
Size: 528 143 15 290 518 2564 2531
Fragments arranged by size:
2564 2531 528 518 290 143 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2380 2412 2639 2671 2733
Size: 32 135 32 808 32 227 32 62
Cuts at: 2733 2765 5230 5262 5304 5336 1373
Size: 32 2465 32 42 32 2626
Fragments arranged by size:
2626 2465 808 227 135 62 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3933 4179 669
Size: 988 281 1995 246 3079
Fragments arranged by size:
3079 1995 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6589
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KasI G'GCGC_C
Cuts at: 2360 3168 2360
Size: 808 5781
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 4188 4188
Size: 6589
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3645 3807 5703 5887 1305
Size: 2340 162 1896 184 2007
Fragments arranged by size:
2340 2007 1896 184 162
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4767
Fragments arranged by size:
4767 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 3558 3707 3875 4547 4906 5065
Size: 648 861 1531 149 168 672 359 159
Cuts at: 5065 518
Size: 2042
Fragments arranged by size:
2042 1531 861 672 648 359 168 159
149
MspA1I CmG'CkG
Cuts at: 1606 2157 2586 2608 2852 2974 2989 3018 3061
Size: 551 429 22 244 122 15 29 43
Cuts at: 3061 3133 3174 4005 4682 5830 6075 1606
Size: 72 41 831 677 1148 245 2120
Fragments arranged by size:
2120 1148 831 677 551 429 245 244
122 72 43 41 29 22 15
NarI GG'CG_CC
Cuts at: 2361 3169 2361
Size: 808 5781
NcoI C'CATG_G
Cuts at: 513 1065 3708 513
Size: 552 2643 3394
Fragments arranged by size:
3394 2643 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 2068 2509 3563 3947 5492 1132
Size: 97 839 441 1054 384 1545 2229
Fragments arranged by size:
2229 1545 1054 839 441 384 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6589
PciI A'CATG_T
Cuts at: 2505 5488 2505
Size: 2983 3606
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2977 3094 3393 1108
Size: 1869 117 299 4304
Fragments arranged by size:
4304 1869 299 117
PfoI T'CCnGG_A
Cuts at: 2712 3117 3300 2712
Size: 405 183 6001
Fragments arranged by size:
6001 405 183
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PmlI CAC'GTG
Cuts at: 3207 3207
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 6171 6203 4658
Size: 32 1481 32 5044
Fragments arranged by size:
5044 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2515 2515
Size: 6589
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 4159 4159
Size: 6589
PstI C_TGCA'G
Cuts at: 830 2964 3941 830
Size: 2134 977 3478
Fragments arranged by size:
3478 2134 977
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
PvuII CAG'CTG
Cuts at: 2608 2974 2989 3061 2608
Size: 366 15 72 6136
Fragments arranged by size:
6136 366 72 15
SacI G_AGCT'C
Cuts at: 2328 2328
Size: 6589
SacII CC_GC'GG
Cuts at: 3019 3019
Size: 6589
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 4837 6549 4837
Size: 1712 4877
SexAI A'CCwGG_T
Cuts at: 3414 3414
Size: 6589
SfcI C'TryA_G
Cuts at: 826 1045 2960 3937 5072 5753 5944 826
Size: 219 1915 977 1135 681 191 1471
Fragments arranged by size:
1915 1471 1135 977 681 219 191
SfoI GGC'GCC
Cuts at: 2362 3170 2362
Size: 808 5781
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 3916 1872
Size: 2044 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2528 2744 4693 5594 5856 820
Size: 197 926 585 216 1949 901 262 1553
Fragments arranged by size:
1949 1553 926 901 585 262 216 197
SnaBI TAC'GTA
Cuts at: 493 3886 4380 493
Size: 3393 494 2702
Fragments arranged by size:
3393 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6589
SphI G_CATG'C
Cuts at: 1132 2068 3947 1132
Size: 936 1879 3774
Fragments arranged by size:
3774 1879 936
SspI AAT'ATT
Cuts at: 5 52 4513 5
Size: 47 4461 2081
Fragments arranged by size:
4461 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6589
StyI C'CwwG_G
Cuts at: 513 1065 2254 3708 4028 513
Size: 552 1189 1454 320 3074
Fragments arranged by size:
3074 1454 1189 552 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2806 3148 4616 4633 4786 4971 5416
Size: 1255 1226 342 1468 17 153 185 445
Cuts at: 5416 325
Size: 1498
Fragments arranged by size:
1498 1468 1255 1226 445 342 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 4835 6547
Size: 274 80 33 51 1443 543 2315 1712
Cuts at: 6547 96
Size: 138
Fragments arranged by size:
2315 1712 1443 543 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6549 6581 6549
Size: 32 6557
Tth111I GACn'n_nGTC
Cuts at: 1316 2558 1316
Size: 1242 5347
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2077 2104 2077
Size: 27 6562
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6589
XmaI C'CCGG_G
Cuts at: 1870 3914 1870
Size: 2044 4545
XmnI GAAnn'nnTTC
Cuts at: 4718 4718
Size: 6589
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2820 4397 276
Size: 53 83 186 936 1286 1577 2468
Fragments arranged by size:
2468 1577 1286 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BfrBI BplI BsiWI BstZ17I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SanDI SapI SfiI
SrfI SwaI