(Circular) (Six-base) MAPSORT of: pFN21ASDB0036.seq Check: 3438 from: 1 to: 6664 pFN21ASDB0036 6664 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4474 278 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2384 4007 1937 Size: 447 1623 4594 Fragments arranged by size: 4594 1623 447 Acc65I G'GTAC_C Cuts at: 3985 3985 Size: 6664 AclI AA'CG_TT Cuts at: 1563 4791 5164 1563 Size: 3228 373 3063 Fragments arranged by size: 3228 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3342 3429 4727 6111 6379 1632 Size: 1710 87 1298 1384 268 1917 Fragments arranged by size: 1917 1710 1384 1298 268 87 AflII C'TTAA_G Cuts at: 820 1017 2338 820 Size: 197 1321 5146 Fragments arranged by size: 5146 1321 197 AflIII A'CryG_T Cuts at: 1203 2325 2379 5563 6540 1203 Size: 1122 54 3184 977 1327 Fragments arranged by size: 3184 1327 1122 977 54 AgeI A'CCGG_T Cuts at: 5495 5495 Size: 6664 AhdI GACnn_n'nnGTC Cuts at: 5393 5393 Size: 6664 AleI CACnn'nnGTG Cuts at: 1166 2462 1166 Size: 1296 5368 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6664 AlwNI CAG_nnn'CTG Cuts at: 2038 4407 5979 2038 Size: 2369 1572 2723 Fragments arranged by size: 2723 2369 1572 ApaI G_GGCC'C Cuts at: 2133 2133 Size: 6664 ApaLI G'TGCA_C Cuts at: 2164 4721 5877 2164 Size: 2557 1156 2951 Fragments arranged by size: 2951 2557 1156 ApoI r'AATT_y Cuts at: 1493 3837 3973 4199 4351 6460 1493 Size: 2344 136 226 152 2109 1697 Fragments arranged by size: 2344 2109 1697 226 152 136 AseI AT'TA_AT Cuts at: 160 1032 2670 5218 160 Size: 872 1638 2548 1606 Fragments arranged by size: 2548 1638 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 AvaI C'yCGr_G Cuts at: 1870 1943 3989 1870 Size: 73 2046 4545 Fragments arranged by size: 4545 2046 73 BamHI G'GATC_C Cuts at: 3994 4361 3994 Size: 367 6297 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3781 3985 5440 618 Size: 325 217 627 1994 204 1455 1842 Fragments arranged by size: 1994 1842 1455 627 325 217 204 BanII G_rGCy'C Cuts at: 1391 2133 3983 1391 Size: 742 1850 4072 Fragments arranged by size: 4072 1850 742 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2239 2834 2934 928 Size: 971 340 595 100 4658 Fragments arranged by size: 4658 971 595 340 100 BbvCI CC'TCA_GC Cuts at: 3112 3112 Size: 6664 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4855 4889 1903 Size: 34 2918 34 3678 Fragments arranged by size: 3678 2918 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2098 3115 4556 5766 2098 Size: 1017 1441 1210 2996 Fragments arranged by size: 2996 1441 1210 1017 BclI T'GATC_A Cuts at: 1548 1548 Size: 6664 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5275 136 Size: 107 122 71 631 605 3603 1525 Fragments arranged by size: 3603 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6659 6659 Size: 6664 BlpI GC'TnA_GC Cuts at: 4081 4081 Size: 6664 Bme1580I G_kGCm'C Cuts at: 1790 2133 2168 4725 5881 1790 Size: 343 35 2557 1156 2573 Fragments arranged by size: 2573 2557 1156 343 35 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6664 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3729 4395 5353 Size: 416 53 83 399 24 2312 666 958 Cuts at: 5353 442 Size: 1753 Fragments arranged by size: 2312 1753 958 666 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3408 5324 1239 Size: 2169 1916 2579 Fragments arranged by size: 2579 2169 1916 Bpu10I CC'TnA_GC Cuts at: 3112 3112 Size: 6664 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4789 5654 5952 4789 Size: 865 298 5501 Fragments arranged by size: 5501 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3901 5327 882 Size: 617 2402 1426 2219 Fragments arranged by size: 2402 2219 1426 617 BsaAI yAC'GTr Cuts at: 493 1204 3961 4455 493 Size: 711 2757 494 2702 Fragments arranged by size: 2757 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6658 6658 Size: 6664 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4471 4853 5488 275 Size: 53 83 186 936 2938 382 635 1451 Fragments arranged by size: 2938 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5097 5495 5769 5916 6327 5097 Size: 398 274 147 411 5434 Fragments arranged by size: 5434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3270 3300 3406 3436 1178 Size: 30 165 30 1867 30 106 30 4406 Fragments arranged by size: 4406 1867 165 106 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6664 BseYI C'CCAG_C Cuts at: 2061 5867 6363 2061 Size: 3806 496 2362 Fragments arranged by size: 3806 2362 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3117 1163 Size: 573 1381 4710 Fragments arranged by size: 4710 1381 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4114 4875 5903 1540 Size: 275 186 2113 761 1028 2301 Fragments arranged by size: 2301 2113 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2168 3943 4725 4810 5881 2168 Size: 1775 782 85 1071 2951 Fragments arranged by size: 2951 1775 1071 782 85 BsmI GAATG_Cn' Cuts at: 3772 4175 4268 3772 Size: 403 93 6168 Fragments arranged by size: 6168 403 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2168 3943 3983 4725 4810 5881 Size: 399 343 35 1775 40 742 85 1071 Cuts at: 5881 1391 Size: 2174 Fragments arranged by size: 2174 1775 1071 742 399 343 85 40 35 BspEI T'CCGG_A Cuts at: 6327 6327 Size: 6664 BspHI T'CATG_A Cuts at: 3394 3903 4553 6283 3394 Size: 509 650 1730 3775 Fragments arranged by size: 3775 1730 650 509 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4019 844 Size: 3175 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4551 1394 Size: 429 2728 3507 Fragments arranged by size: 3507 2728 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2584 5159 5333 65 Size: 1183 1336 2575 174 1396 Fragments arranged by size: 2575 1396 1336 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2075 5308 5495 1700 Size: 110 265 3233 187 2869 Fragments arranged by size: 3233 2869 265 187 110 BsrGI T'GTAC_A Cuts at: 96 3235 96 Size: 3139 3525 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6664 BssSI C'ACGA_G Cuts at: 2117 4724 5736 2117 Size: 2607 1012 3045 Fragments arranged by size: 3045 2607 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6664 BstBI TT'CG_AA Cuts at: 2007 5468 2007 Size: 3461 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6664 BstXI CCAn_nnnn'nTGG Cuts at: 3643 3643 Size: 6664 BstYI r'GATC_y Cuts at: 1972 3725 3994 4361 4746 4763 6215 6301 6313 Size: 1753 269 367 385 17 1452 86 12 Cuts at: 6313 6659 1972 Size: 346 1977 Fragments arranged by size: 1977 1753 1452 385 367 346 269 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5925 Fragments arranged by size: 5925 683 56 BtsI GCAGTG_nn' Cuts at: 2052 2943 4180 4974 4994 2052 Size: 891 1237 794 20 3722 Fragments arranged by size: 3722 1237 891 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6664 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6629 DraI TTT'AAA Cuts at: 2415 3036 3968 2415 Size: 621 932 5111 Fragments arranged by size: 5111 932 621 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5671 809 Size: 507 4355 1802 Fragments arranged by size: 4355 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 4111 5000 Size: 54 1425 50 275 18 199 2082 889 Cuts at: 5000 6629 8 Size: 1629 43 Fragments arranged by size: 2082 1629 1425 889 275 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4111 1537 Size: 275 2299 4090 Fragments arranged by size: 4090 2299 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2462 4594 1353 Size: 1109 2132 3423 Fragments arranged by size: 3423 2132 1109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5251 5625 5771 387 Size: 1064 138 3662 374 146 1280 Fragments arranged by size: 3662 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3342 3408 3429 4727 5324 6111 6379 Size: 393 1710 66 21 1298 597 787 268 Cuts at: 6379 1239 Size: 1524 Fragments arranged by size: 1710 1524 1298 787 597 393 268 66 21 EcoNI CCTnn'n_nnAGG Cuts at: 2825 3933 2825 Size: 1108 5556 EcoO109I rG'GnC_Cy Cuts at: 2129 2830 2129 Size: 701 5963 EcoRI G'AATT_C Cuts at: 3837 3973 4351 3837 Size: 136 378 6150 Fragments arranged by size: 6150 378 136 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2738 2738 Size: 6664 FspI TGC'GCA Cuts at: 5170 5170 Size: 6664 HaeII r_GCGC'y Cuts at: 1678 3049 5811 1678 Size: 1371 2762 2531 Fragments arranged by size: 2762 2531 1371 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3376 3408 3455 3487 5305 Size: 32 135 32 1804 32 47 32 1818 Cuts at: 5305 5337 5379 5411 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1818 1804 135 47 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3249 3463 3698 3717 4008 4254 Size: 988 281 1311 214 235 19 291 246 Cuts at: 4254 669 Size: 3079 Fragments arranged by size: 3079 1311 988 291 281 246 235 214 19 HindIII A'AGCT_T Cuts at: 748 3030 3304 748 Size: 2282 274 4108 Fragments arranged by size: 4108 2282 274 HpaI GTT'AAC Cuts at: 3717 4254 3717 Size: 537 6127 KpnI G_GTAC'C Cuts at: 3989 3989 Size: 6664 MfeI C'AATT_G Cuts at: 4263 4263 Size: 6664 MluI A'CGCG_T Cuts at: 6540 6540 Size: 6664 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2658 2773 3388 5778 5962 1305 Size: 1353 115 615 2390 184 2007 Fragments arranged by size: 2390 2007 1353 615 184 115 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4842 Fragments arranged by size: 4842 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2462 4622 4981 5140 518 Size: 648 1296 2160 359 159 2042 Fragments arranged by size: 2160 2042 1296 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2906 3449 4080 4757 5905 6150 1606 Size: 1300 543 631 677 1148 245 2120 Fragments arranged by size: 2120 1300 1148 677 631 543 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6112 NdeI CA'TA_TG Cuts at: 387 387 Size: 6664 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6664 NotI GC'GGCC_GC Cuts at: 4111 4111 Size: 6664 NspI r_CATG'y Cuts at: 1132 1229 2383 4022 5567 1132 Size: 97 1154 1639 1545 2229 Fragments arranged by size: 2229 1639 1545 1154 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6664 PciI A'CATG_T Cuts at: 2379 5563 2379 Size: 3184 3480 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6664 PmeI GTTT'AAAC Cuts at: 3968 3968 Size: 6664 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4733 4765 6246 6278 4733 Size: 32 1481 32 5119 Fragments arranged by size: 5119 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6664 PsiI TTA'TAA Cuts at: 4234 4234 Size: 6664 PspOMI G'GGCC_C Cuts at: 2129 2129 Size: 6664 PstI C_TGCA'G Cuts at: 830 3094 4016 830 Size: 2264 922 3478 Fragments arranged by size: 3478 2264 922 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6664 PvuII CAG'CTG Cuts at: 2906 3449 2906 Size: 543 6121 RsrII CG'GwC_CG Cuts at: 2044 2044 Size: 6664 SalI G'TCGA_C Cuts at: 1936 4006 1936 Size: 2070 4594 SbfI CC_TGCA'GG Cuts at: 4016 4016 Size: 6664 ScaI AGT'ACT Cuts at: 4912 6624 4912 Size: 1712 4952 SexAI A'CCwGG_T Cuts at: 3700 3700 Size: 6664 SfcI C'TryA_G Cuts at: 826 1045 2901 3090 4012 5147 5828 6019 826 Size: 219 1856 189 922 1135 681 191 1471 Fragments arranged by size: 1856 1471 1135 922 681 219 191 189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6664 SmaI CCC'GGG Cuts at: 1872 3991 1872 Size: 2119 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2338 4768 5669 5931 820 Size: 197 926 395 2430 901 262 1553 Fragments arranged by size: 2430 1553 926 901 395 262 197 SnaBI TAC'GTA Cuts at: 493 3961 4455 493 Size: 3468 494 2702 Fragments arranged by size: 3468 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6664 SphI G_CATG'C Cuts at: 1132 4022 1132 Size: 2890 3774 SspI AAT'ATT Cuts at: 5 52 2687 4588 5 Size: 47 2635 1901 2081 Fragments arranged by size: 2635 2081 1901 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6664 StyI C'CwwG_G Cuts at: 513 1065 2874 4103 513 Size: 552 1809 1229 3074 Fragments arranged by size: 3074 1809 1229 552 SwaI ATTT'AAAT Cuts at: 2415 2415 Size: 6664 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2061 2227 3243 4691 4708 4861 5046 Size: 1255 481 166 1016 1448 17 153 185 Cuts at: 5046 5491 325 Size: 445 1498 Fragments arranged by size: 1498 1448 1255 1016 481 445 185 166 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3235 3269 4910 Size: 274 80 33 51 1443 1258 34 1641 Cuts at: 4910 6622 96 Size: 1712 138 Fragments arranged by size: 1712 1641 1443 1258 274 138 80 51 34 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3406 3438 6624 6656 3406 Size: 32 3186 32 3414 Fragments arranged by size: 3414 3186 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6664 XbaI T'CTAG_A Cuts at: 4000 4000 Size: 6664 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3759 3759 Size: 6664 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6664 XmaI C'CCGG_G Cuts at: 1870 3989 1870 Size: 2119 4545 XmnI GAAnn'nnTTC Cuts at: 4793 4793 Size: 6664 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4472 276 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacI SacII SanDI SapI SfiI SfoI SrfI