(Circular) (Six-base) MAPSORT of: pFN21ASDB0035.seq Check: 1835 from: 1 to: 6136 pFN21ASDB0035 6136 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3946 278 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 1937 Size: 1542 4594 Acc65I G'GTAC_C Cuts at: 2259 2662 3055 3457 2259 Size: 403 393 402 4938 Fragments arranged by size: 4938 403 402 393 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3186 4199 5583 5851 1632 Size: 1554 1013 1384 268 1917 Fragments arranged by size: 1917 1554 1384 1013 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5939 AflIII A'CryG_T Cuts at: 1203 5035 6012 1203 Size: 3832 977 1327 Fragments arranged by size: 3832 1327 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 4865 4865 Size: 6136 AleI CACnn'nnGTG Cuts at: 1166 2269 1166 Size: 1103 5033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6136 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2449 2481 2449 Size: 32 6104 AlwNI CAG_nnn'CTG Cuts at: 2828 3040 3086 3110 3879 5451 2828 Size: 212 46 24 769 1572 3513 Fragments arranged by size: 3513 1572 769 212 46 24 ApaLI G'TGCA_C Cuts at: 4193 5349 4193 Size: 1156 4980 ApoI r'AATT_y Cuts at: 1493 2113 2296 2439 3273 3302 3329 3445 3671 Size: 620 183 143 834 29 27 116 226 Cuts at: 3671 3823 5932 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 834 620 226 183 152 143 116 29 27 AseI AT'TA_AT Cuts at: 160 1032 4690 160 Size: 872 3658 1606 Fragments arranged by size: 3658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 2736 3461 1870 Size: 73 793 725 4545 Fragments arranged by size: 4545 793 725 73 BamHI G'GATC_C Cuts at: 3466 3833 3466 Size: 367 5769 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2259 2662 2780 3055 3170 Size: 325 217 627 472 403 118 275 115 Cuts at: 3170 3457 4912 618 Size: 287 1455 1842 Fragments arranged by size: 1842 1455 627 472 403 325 287 275 217 118 115 BanII G_rGCy'C Cuts at: 1391 2646 3455 1391 Size: 1255 809 4072 Fragments arranged by size: 4072 1255 809 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 2556 2749 928 Size: 971 317 340 193 4315 Fragments arranged by size: 4315 971 340 317 193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4327 4361 1903 Size: 34 2390 34 3678 Fragments arranged by size: 3678 2390 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 1548 Size: 6136 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4747 136 Size: 107 122 71 631 605 3075 1525 Fragments arranged by size: 3075 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6131 6131 Size: 6136 BlpI GC'TnA_GC Cuts at: 3553 3553 Size: 6136 Bme1580I G_kGCm'C Cuts at: 1790 2783 3175 4197 5353 1790 Size: 993 392 1022 1156 2573 Fragments arranged by size: 2573 1156 1022 993 392 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6136 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2793 3867 4825 Size: 416 53 83 399 24 1376 1074 958 Cuts at: 4825 442 Size: 1753 Fragments arranged by size: 1753 1376 1074 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2850 3082 4796 1239 Size: 1611 232 1714 2579 Fragments arranged by size: 2579 1714 1611 232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2921 4261 5126 5424 2921 Size: 1340 865 298 3633 Fragments arranged by size: 3633 1340 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4799 882 Size: 617 3300 2219 Fragments arranged by size: 3300 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3433 3927 493 Size: 711 2229 494 2702 Fragments arranged by size: 2702 2229 711 494 BsaBI GATnn'nnATC Cuts at: 6130 6130 Size: 6136 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3943 4325 4960 275 Size: 53 83 186 936 2410 382 635 1451 Fragments arranged by size: 2410 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3154 4569 4967 5241 5388 5799 3154 Size: 1415 398 274 147 411 3491 Fragments arranged by size: 3491 1415 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2912 2942 1178 Size: 30 165 30 1509 30 4372 Fragments arranged by size: 4372 1509 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2715 2736 1186 Size: 1529 21 4586 Fragments arranged by size: 4586 1529 21 BseYI C'CCAG_C Cuts at: 5339 5835 5339 Size: 496 5640 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5563 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3586 4347 5375 1540 Size: 275 186 1585 761 1028 2301 Fragments arranged by size: 2301 1585 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2646 4197 4282 5353 2646 Size: 1551 85 1071 3429 Fragments arranged by size: 3429 1551 1071 85 BsmI GAATG_Cn' Cuts at: 2053 3647 3740 2053 Size: 1594 93 4449 Fragments arranged by size: 4449 1594 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2646 2783 3175 3455 4197 4282 5353 Size: 399 856 137 392 280 742 85 1071 Cuts at: 5353 1391 Size: 2174 Fragments arranged by size: 2174 1071 856 742 399 392 280 137 85 BspEI T'CCGG_A Cuts at: 5799 5799 Size: 6136 BspHI T'CATG_A Cuts at: 4025 5755 4025 Size: 1730 4406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3491 844 Size: 2647 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4023 1394 Size: 429 2200 3507 Fragments arranged by size: 3507 2200 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3223 4631 4805 65 Size: 1183 1975 1408 174 1396 Fragments arranged by size: 1975 1408 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4780 4967 1700 Size: 110 2970 187 2869 Fragments arranged by size: 2970 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6136 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6136 BssSI C'ACGA_G Cuts at: 4196 5208 4196 Size: 1012 5124 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6136 BstBI TT'CG_AA Cuts at: 2007 4940 2007 Size: 2933 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6136 BstXI CCAn_nnnn'nTGG Cuts at: 2271 2451 2271 Size: 180 5956 BstYI r'GATC_y Cuts at: 1972 2457 2624 2886 3466 3833 4218 4235 5687 Size: 485 167 262 580 367 385 17 1452 Cuts at: 5687 5773 5785 6131 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 580 485 385 367 346 262 167 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3051 3365 3051 Size: 314 5822 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5584 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5397 Fragments arranged by size: 5397 683 56 BtsI GCAGTG_nn' Cuts at: 3196 3652 4446 4466 3196 Size: 456 794 20 4866 Fragments arranged by size: 4866 794 456 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6101 DraI TTT'AAA Cuts at: 3440 3440 Size: 6136 DraIII CAC_nnn'GTG Cuts at: 2207 2207 Size: 6136 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3005 5143 809 Size: 507 1689 2138 1802 Fragments arranged by size: 2138 1802 1689 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3264 3583 4472 Size: 54 1425 50 275 18 1434 319 889 Cuts at: 4472 6101 8 Size: 1629 43 Fragments arranged by size: 1629 1434 1425 889 319 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3583 1537 Size: 275 1771 4090 Fragments arranged by size: 4090 1771 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4066 1353 Size: 2713 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2899 4723 5097 5243 387 Size: 1064 138 1310 1824 374 146 1280 Fragments arranged by size: 1824 1310 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2644 2644 Size: 6136 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2850 3082 3186 4199 4796 5583 5851 Size: 393 1218 232 104 1013 597 787 268 Cuts at: 5851 1239 Size: 1524 Fragments arranged by size: 1524 1218 1013 787 597 393 268 232 104 EcoRI G'AATT_C Cuts at: 2296 2439 3445 3823 2296 Size: 143 1006 378 4609 Fragments arranged by size: 4609 1006 378 143 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2397 3191 2397 Size: 794 5342 FspI TGC'GCA Cuts at: 4642 4642 Size: 6136 HaeII r_GCGC'y Cuts at: 1678 2537 5283 1678 Size: 859 2746 2531 Fragments arranged by size: 2746 2531 859 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3104 3136 4777 4809 4851 Size: 32 135 32 1532 32 1641 32 42 Cuts at: 4851 4883 1373 Size: 32 2626 Fragments arranged by size: 2626 1641 1532 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2404 3480 3726 669 Size: 988 281 466 1076 246 3079 Fragments arranged by size: 3079 1076 988 466 281 246 HindIII A'AGCT_T Cuts at: 748 3376 748 Size: 2628 3508 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KpnI G_GTAC'C Cuts at: 2263 2666 3059 3461 2263 Size: 403 393 402 4938 Fragments arranged by size: 4938 403 402 393 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2332 2955 5250 5434 1305 Size: 1027 623 2295 184 2007 Fragments arranged by size: 2295 2007 1027 623 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4314 Fragments arranged by size: 4314 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2269 2474 4094 4453 4612 518 Size: 648 1103 205 1620 359 159 2042 Fragments arranged by size: 2042 1620 1103 648 359 205 159 MspA1I CmG'CkG Cuts at: 1606 2621 2791 3552 4229 5377 5622 1606 Size: 1015 170 761 677 1148 245 2120 Fragments arranged by size: 2120 1148 1015 761 677 245 170 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5584 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 2487 3095 3494 5039 1132 Size: 97 1258 608 399 1545 2229 Fragments arranged by size: 2229 1545 1258 608 399 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6136 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6136 PfoI T'CCnGG_A Cuts at: 3009 3009 Size: 6136 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 5718 5750 4205 Size: 32 1481 32 4591 Fragments arranged by size: 4591 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2220 3252 1613 Size: 607 1032 4497 Fragments arranged by size: 4497 1032 607 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PstI C_TGCA'G Cuts at: 830 3247 3488 830 Size: 2417 241 3478 Fragments arranged by size: 3478 2417 241 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 SacI G_AGCT'C Cuts at: 2646 2646 Size: 6136 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SbfI CC_TGCA'GG Cuts at: 3488 3488 Size: 6136 ScaI AGT'ACT Cuts at: 4384 6096 4384 Size: 1712 4424 SfcI C'TryA_G Cuts at: 826 1045 3243 3484 4619 5300 5491 826 Size: 219 2198 241 1135 681 191 1471 Fragments arranged by size: 2198 1471 1135 681 241 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 2738 3463 1872 Size: 866 725 4545 Fragments arranged by size: 4545 866 725 SmlI C'TyrA_G Cuts at: 820 1017 1943 2900 4240 5141 5403 820 Size: 197 926 957 1340 901 262 1553 Fragments arranged by size: 1553 1340 957 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3433 3927 493 Size: 2940 494 2702 Fragments arranged by size: 2940 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6136 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 4060 5 Size: 47 4008 2081 Fragments arranged by size: 4008 2081 47 StuI AGG'CCT Cuts at: 1517 3422 1517 Size: 1905 4231 StyI C'CwwG_G Cuts at: 513 1065 3575 513 Size: 552 2510 3074 Fragments arranged by size: 3074 2510 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4163 4180 4333 4518 4963 325 Size: 1255 2583 17 153 185 445 1498 Fragments arranged by size: 2583 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4382 6094 96 Size: 274 80 33 51 1443 2405 1712 138 Fragments arranged by size: 2405 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6096 6128 6096 Size: 32 6104 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6136 XbaI T'CTAG_A Cuts at: 2857 3472 2857 Size: 615 5521 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6136 XmaI C'CCGG_G Cuts at: 1870 2736 3461 1870 Size: 866 725 4545 Fragments arranged by size: 4545 866 725 XmnI GAAnn'nnTTC Cuts at: 2691 4265 2691 Size: 1574 4562 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3944 276 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI