(Circular) (Six-base) MAPSORT of: pFN21ASDB0032.seq Check: 1355 from: 1 to: 6166 pFN21ASDB0032 6166 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2944 2944 Size: 6166 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2938 3976 278 Size: 53 83 186 936 1402 1038 2468 Fragments arranged by size: 2468 1402 1038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3459 3509 1937 Size: 1522 50 4594 Fragments arranged by size: 4594 1522 50 Acc65I G'GTAC_C Cuts at: 3487 3487 Size: 6166 AclI AA'CG_TT Cuts at: 1563 4293 4666 1563 Size: 2730 373 3063 Fragments arranged by size: 3063 2730 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2597 3053 3265 3378 4229 5613 5881 1632 Size: 965 456 212 113 851 1384 268 1917 Fragments arranged by size: 1917 1384 965 851 456 268 212 113 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5969 AflIII A'CryG_T Cuts at: 1203 5065 6042 1203 Size: 3862 977 1327 Fragments arranged by size: 3862 1327 977 AgeI A'CCGG_T Cuts at: 4997 4997 Size: 6166 AhdI GACnn_n'nnGTC Cuts at: 4895 4895 Size: 6166 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6166 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2136 1184 Size: 952 5214 AlwNI CAG_nnn'CTG Cuts at: 3909 5481 3909 Size: 1572 4594 ApaLI G'TGCA_C Cuts at: 4223 5379 4223 Size: 1156 5010 ApoI r'AATT_y Cuts at: 1493 2393 2868 2942 3159 3475 3701 3853 5962 Size: 900 475 74 217 316 226 152 2109 Cuts at: 5962 1493 Size: 1697 Fragments arranged by size: 2109 1697 900 475 316 226 217 152 74 AseI AT'TA_AT Cuts at: 160 1032 4720 160 Size: 872 3688 1606 Fragments arranged by size: 3688 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 AvaI C'yCGr_G Cuts at: 1870 1943 2649 3491 1870 Size: 73 706 842 4545 Fragments arranged by size: 4545 842 706 73 BamHI G'GATC_C Cuts at: 3496 3863 3496 Size: 367 5799 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3487 4942 618 Size: 325 217 627 1700 1455 1842 Fragments arranged by size: 1842 1700 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3485 1391 Size: 2094 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3092 928 Size: 971 1193 4002 Fragments arranged by size: 4002 1193 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4357 4391 1903 Size: 34 2420 34 3678 Fragments arranged by size: 3678 2420 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4058 5268 4058 Size: 1210 4956 BclI T'GATC_A Cuts at: 1548 1548 Size: 6166 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4777 136 Size: 107 122 71 631 605 3105 1525 Fragments arranged by size: 3105 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2358 2486 6161 2358 Size: 128 3675 2363 Fragments arranged by size: 3675 2363 128 BlpI GC'TnA_GC Cuts at: 2109 3583 2109 Size: 1474 4692 Bme1580I G_kGCm'C Cuts at: 1790 4227 5383 1790 Size: 2437 1156 2573 Fragments arranged by size: 2573 2437 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6166 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2342 3897 4855 Size: 416 53 83 399 24 925 1555 958 Cuts at: 4855 442 Size: 1753 Fragments arranged by size: 1753 1555 958 925 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3387 4826 1239 Size: 2148 1439 2579 Fragments arranged by size: 2579 2148 1439 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3101 4291 5156 5454 3101 Size: 1190 865 298 3813 Fragments arranged by size: 3813 1190 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4829 882 Size: 617 3330 2219 Fragments arranged by size: 3330 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3463 3957 493 Size: 711 2259 494 2702 Fragments arranged by size: 2702 2259 711 494 BsaBI GATnn'nnATC Cuts at: 3183 6160 3183 Size: 2977 3189 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2935 3973 4355 4990 Size: 53 83 186 936 1402 1038 382 635 Cuts at: 4990 275 Size: 1451 Fragments arranged by size: 1451 1402 1038 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4599 4997 5271 5418 5829 4599 Size: 398 274 147 411 4936 Fragments arranged by size: 4936 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5941 Fragments arranged by size: 5941 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2481 1186 Size: 1295 4871 BseYI C'CCAG_C Cuts at: 5369 5865 5369 Size: 496 5670 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 1163 Size: 573 336 5257 Fragments arranged by size: 5257 573 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3616 4377 5405 1540 Size: 275 186 1615 761 1028 2301 Fragments arranged by size: 2301 1615 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4227 4312 5383 4227 Size: 85 1071 5010 Fragments arranged by size: 5010 1071 85 BsmI GAATG_Cn' Cuts at: 2931 3677 3770 2931 Size: 746 93 5327 Fragments arranged by size: 5327 746 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3485 4227 4312 5383 1391 Size: 399 1695 742 85 1071 2174 Fragments arranged by size: 2174 1695 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5829 5829 Size: 6166 BspHI T'CATG_A Cuts at: 4055 5785 4055 Size: 1730 4436 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2944 3521 844 Size: 2100 577 3489 Fragments arranged by size: 3489 2100 577 BsrBI CCG'CTC Cuts at: 1394 1823 4053 1394 Size: 429 2230 3507 Fragments arranged by size: 3507 2230 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2341 2448 2974 3036 4661 4835 65 Size: 1183 1093 107 526 62 1625 174 1396 Fragments arranged by size: 1625 1396 1183 1093 526 174 107 62 BsrFI r'CCGG_y Cuts at: 1700 1810 4810 4997 1700 Size: 110 3000 187 2869 Fragments arranged by size: 3000 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2325 96 Size: 2229 3937 BssHII G'CGCG_C Cuts at: 1925 2713 1925 Size: 788 5378 BssSI C'ACGA_G Cuts at: 4226 5238 4226 Size: 1012 5154 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6166 BstBI TT'CG_AA Cuts at: 2007 4970 2007 Size: 2963 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6166 BstYI r'GATC_y Cuts at: 1972 2275 2358 2486 3496 3863 4248 4265 5717 Size: 303 83 128 1010 367 385 17 1452 Cuts at: 5717 5803 5815 6161 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1010 385 367 346 303 128 86 83 17 12 BstZ17I GTA'TAC Cuts at: 3460 3460 Size: 6166 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5614 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5427 Fragments arranged by size: 5427 683 56 BtsI GCAGTG_nn' Cuts at: 2459 3682 4476 4496 2459 Size: 1223 794 20 4129 Fragments arranged by size: 4129 1223 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6166 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6131 DraI TTT'AAA Cuts at: 2791 3372 3470 2791 Size: 581 98 5487 Fragments arranged by size: 5487 581 98 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5173 809 Size: 507 3857 1802 Fragments arranged by size: 3857 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2833 3613 4502 Size: 54 1425 50 275 18 1003 780 889 Cuts at: 4502 6131 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1003 889 780 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3613 1537 Size: 275 1801 4090 Fragments arranged by size: 4090 1801 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2763 4096 1353 Size: 1410 1333 3423 Fragments arranged by size: 3423 1410 1333 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4753 5127 5273 387 Size: 1064 138 3164 374 146 1280 Fragments arranged by size: 3164 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2597 3053 3265 3378 3387 4229 4826 Size: 393 965 456 212 113 9 842 597 Cuts at: 4826 5613 5881 1239 Size: 787 268 1524 Fragments arranged by size: 1524 965 842 787 597 456 393 268 212 113 9 EcoO109I rG'GnC_Cy Cuts at: 2990 2990 Size: 6166 EcoRI G'AATT_C Cuts at: 3159 3475 3853 3159 Size: 316 378 5472 Fragments arranged by size: 5472 378 316 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3179 3179 Size: 6166 FspI TGC'GCA Cuts at: 4672 4672 Size: 6166 HaeII r_GCGC'y Cuts at: 1678 5313 1678 Size: 3635 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4807 4839 4881 4913 1373 Size: 32 135 32 3235 32 42 32 2626 Fragments arranged by size: 3235 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2188 3510 3756 669 Size: 988 281 250 1322 246 3079 Fragments arranged by size: 3079 1322 988 281 250 246 HindIII A'AGCT_T Cuts at: 748 2130 2638 748 Size: 1382 508 4276 Fragments arranged by size: 4276 1382 508 HpaI GTT'AAC Cuts at: 2188 3756 2188 Size: 1568 4598 KpnI G_GTAC'C Cuts at: 3491 3491 Size: 6166 MfeI C'AATT_G Cuts at: 3315 3765 3315 Size: 450 5716 MluI A'CGCG_T Cuts at: 6042 6042 Size: 6166 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2237 5280 5464 1305 Size: 932 3043 184 2007 Fragments arranged by size: 3043 2007 932 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2835 10 Size: 54 1425 343 1003 3341 Fragments arranged by size: 3341 1425 1003 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4124 4483 4642 518 Size: 648 2958 359 159 2042 Fragments arranged by size: 2958 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 3356 3582 4259 5407 5652 1606 Size: 450 1300 226 677 1148 245 2120 Fragments arranged by size: 2120 1300 1148 677 450 245 226 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5614 NdeI CA'TA_TG Cuts at: 387 2631 387 Size: 2244 3922 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6166 NotI GC'GGCC_GC Cuts at: 3613 3613 Size: 6166 NspI r_CATG'y Cuts at: 1132 1229 2326 3524 5069 1132 Size: 97 1097 1198 1545 2229 Fragments arranged by size: 2229 1545 1198 1097 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6166 PciI A'CATG_T Cuts at: 5065 5065 Size: 6166 PflMI CCAn_nnn'nTGG Cuts at: 1108 2831 3122 1108 Size: 1723 291 4152 Fragments arranged by size: 4152 1723 291 PmeI GTTT'AAAC Cuts at: 3470 3470 Size: 6166 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4235 4267 5748 5780 4235 Size: 32 1481 32 4621 Fragments arranged by size: 4621 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2990 2990 Size: 6166 PshAI GACnn'nnGTC Cuts at: 1613 2726 1613 Size: 1113 5053 PsiI TTA'TAA Cuts at: 3051 3736 3051 Size: 685 5481 PspXI vC'TCGA_Gb Cuts at: 2649 2649 Size: 6166 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3334 3366 3334 Size: 32 6134 PstI C_TGCA'G Cuts at: 830 2466 2677 3025 3518 830 Size: 1636 211 348 493 3478 Fragments arranged by size: 3478 1636 493 348 211 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6166 PvuII CAG'CTG Cuts at: 2056 3356 2056 Size: 1300 4866 SalI G'TCGA_C Cuts at: 1936 3508 1936 Size: 1572 4594 SbfI CC_TGCA'GG Cuts at: 3518 3518 Size: 6166 ScaI AGT'ACT Cuts at: 4414 6126 4414 Size: 1712 4454 SfcI C'TryA_G Cuts at: 826 1045 2263 2379 2462 2673 2979 3021 3351 Size: 219 1218 116 83 211 306 42 330 Cuts at: 3351 3514 4649 5330 5521 826 Size: 163 1135 681 191 1471 Fragments arranged by size: 1471 1218 1135 681 330 306 219 211 191 163 116 83 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6166 SmaI CCC'GGG Cuts at: 1872 3493 1872 Size: 1621 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2649 3080 4270 5171 5433 820 Size: 197 926 706 431 1190 901 262 1553 Fragments arranged by size: 1553 1190 926 901 706 431 262 197 SnaBI TAC'GTA Cuts at: 493 3463 3957 493 Size: 2970 494 2702 Fragments arranged by size: 2970 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6166 SphI G_CATG'C Cuts at: 1132 3524 1132 Size: 2392 3774 SspI AAT'ATT Cuts at: 5 52 3431 4090 5 Size: 47 3379 659 2081 Fragments arranged by size: 3379 2081 659 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6166 StyI C'CwwG_G Cuts at: 513 1065 2836 3605 513 Size: 552 1771 769 3074 Fragments arranged by size: 3074 1771 769 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2188 3262 4193 4210 4363 4548 4993 Size: 1255 608 1074 931 17 153 185 445 Cuts at: 4993 325 Size: 1498 Fragments arranged by size: 1498 1255 1074 931 608 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 3406 4412 Size: 274 80 33 51 1443 348 1081 1006 Cuts at: 4412 6124 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1081 1006 348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2502 2534 6126 6158 2502 Size: 32 3592 32 2510 Fragments arranged by size: 3592 2510 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6166 XbaI T'CTAG_A Cuts at: 3502 3502 Size: 6166 XhoI C'TCGA_G Cuts at: 1943 2649 1943 Size: 706 5460 XmaI C'CCGG_G Cuts at: 1870 3491 1870 Size: 1621 4545 XmnI GAAnn'nnTTC Cuts at: 2475 3346 4295 2475 Size: 871 949 4346 Fragments arranged by size: 4346 949 871 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2936 3974 276 Size: 53 83 186 936 1402 1038 2468 Fragments arranged by size: 2468 1402 1038 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI