(Circular) (Six-base) MAPSORT of: pFN21ASDB0029.seq Check: 5822 from: 1 to: 6439 pFN21ASDB0029 6439 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2306 2588 2306 Size: 282 6157 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4249 278 Size: 53 83 186 936 2713 2468 Fragments arranged by size: 2713 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3456 3782 1937 Size: 1519 326 4594 Fragments arranged by size: 4594 1519 326 Acc65I G'GTAC_C Cuts at: 3760 3760 Size: 6439 AclI AA'CG_TT Cuts at: 1563 4566 4939 1563 Size: 3003 373 3063 Fragments arranged by size: 3063 3003 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3013 4502 5886 6154 1632 Size: 1381 1489 1384 268 1917 Fragments arranged by size: 1917 1489 1384 1381 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6242 AflIII A'CryG_T Cuts at: 1203 5338 6315 1203 Size: 4135 977 1327 Fragments arranged by size: 4135 1327 977 AgeI A'CCGG_T Cuts at: 5270 5270 Size: 6439 AhdI GACnn_n'nnGTC Cuts at: 5168 5168 Size: 6439 AleI CACnn'nnGTG Cuts at: 1166 3653 1166 Size: 2487 3952 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3395 1184 Size: 2211 4228 AlwNI CAG_nnn'CTG Cuts at: 3269 3575 4182 5754 3269 Size: 306 607 1572 3954 Fragments arranged by size: 3954 1572 607 306 ApaLI G'TGCA_C Cuts at: 4496 5652 4496 Size: 1156 5283 ApoI r'AATT_y Cuts at: 1493 3748 3974 4126 6235 1493 Size: 2255 226 152 2109 1697 Fragments arranged by size: 2255 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4993 160 Size: 872 3961 1606 Fragments arranged by size: 3961 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 AvaI C'yCGr_G Cuts at: 1870 1943 3579 3764 1870 Size: 73 1636 185 4545 Fragments arranged by size: 4545 1636 185 73 BamHI G'GATC_C Cuts at: 3259 3769 4136 3259 Size: 510 367 5562 Fragments arranged by size: 5562 510 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2321 2347 3083 3560 3647 Size: 325 217 627 534 26 736 477 87 Cuts at: 3647 3694 3760 5215 618 Size: 47 66 1455 1842 Fragments arranged by size: 1842 1455 736 627 534 477 325 217 87 66 47 26 BanII G_rGCy'C Cuts at: 1391 2565 2856 2948 3349 3587 3758 1391 Size: 1174 291 92 401 238 171 4072 Fragments arranged by size: 4072 1174 401 291 238 171 92 BbeI G_GCGC'C Cuts at: 3698 3698 Size: 6439 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2195 928 Size: 971 296 5172 Fragments arranged by size: 5172 971 296 BbvCI CC'TCA_GC Cuts at: 2721 2721 Size: 6439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3670 3704 4630 4664 1903 Size: 34 1733 34 926 34 3678 Fragments arranged by size: 3678 1733 926 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3216 4331 5541 3216 Size: 1115 1210 4114 Fragments arranged by size: 4114 1210 1115 BclI T'GATC_A Cuts at: 1548 2104 2331 1548 Size: 556 227 5656 Fragments arranged by size: 5656 556 227 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2685 2915 3645 Size: 107 122 71 631 605 1013 230 730 Cuts at: 3645 5050 136 Size: 1405 1525 Fragments arranged by size: 1525 1405 1013 730 631 605 230 122 107 71 BglII A'GATC_T Cuts at: 2571 3192 6434 2571 Size: 621 3242 2576 Fragments arranged by size: 3242 2576 621 BlpI GC'TnA_GC Cuts at: 3673 3856 3673 Size: 183 6256 Bme1580I G_kGCm'C Cuts at: 1790 3019 3086 4500 5656 1790 Size: 1229 67 1414 1156 2573 Fragments arranged by size: 2573 1414 1229 1156 67 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6439 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2914 4170 5128 Size: 416 53 83 399 24 1497 1256 958 Cuts at: 5128 442 Size: 1753 Fragments arranged by size: 1753 1497 1256 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6439 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2199 2244 5099 1239 Size: 960 45 2855 2579 Fragments arranged by size: 2855 2579 960 45 Bpu10I CC'TnA_GC Cuts at: 2311 2352 2721 2942 2311 Size: 41 369 221 5808 Fragments arranged by size: 5808 369 221 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2592 4564 5429 5727 2592 Size: 1972 865 298 3304 Fragments arranged by size: 3304 1972 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5102 882 Size: 617 3603 2219 Fragments arranged by size: 3603 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3403 3736 4230 493 Size: 711 2199 333 494 2702 Fragments arranged by size: 2702 2199 711 494 333 BsaBI GATnn'nnATC Cuts at: 2216 2541 6433 2216 Size: 325 3892 2222 Fragments arranged by size: 3892 2222 325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3695 4246 4628 5263 Size: 53 83 186 936 2162 551 382 635 Cuts at: 5263 275 Size: 1451 Fragments arranged by size: 2162 1451 936 635 551 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2962 4872 5270 5544 5691 6102 2962 Size: 1910 398 274 147 411 3299 Fragments arranged by size: 3299 1910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2960 2990 3092 3122 1178 Size: 30 165 30 1557 30 102 30 4495 Fragments arranged by size: 4495 1557 165 102 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2196 3113 3339 1186 Size: 1010 917 226 4286 Fragments arranged by size: 4286 1010 917 226 BseYI C'CCAG_C Cuts at: 2593 5642 6138 2593 Size: 3049 496 2894 Fragments arranged by size: 3049 2894 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2498 2820 3007 3383 1163 Size: 573 762 322 187 376 4219 Fragments arranged by size: 4219 762 573 376 322 187 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3599 3709 3889 4650 5678 1540 Size: 275 186 1598 110 180 761 1028 2301 Fragments arranged by size: 2301 1598 1028 761 275 186 180 110 BsiHKAI G_wGCw'C Cuts at: 2565 4500 4585 5656 2565 Size: 1935 85 1071 3348 Fragments arranged by size: 3348 1935 1071 85 BsmI GAATG_Cn' Cuts at: 3950 4043 3950 Size: 93 6346 BsmBI CGTCTCn'nnnn_ Cuts at: 2075 3286 2075 Size: 1211 5228 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2565 2856 2948 3019 3086 3349 3587 Size: 399 775 291 92 71 67 263 238 Cuts at: 3587 3758 4500 4585 5656 1391 Size: 171 742 85 1071 2174 Fragments arranged by size: 2174 1071 775 742 399 291 263 238 171 92 85 71 67 BspEI T'CCGG_A Cuts at: 2962 6102 2962 Size: 3140 3299 BspHI T'CATG_A Cuts at: 2787 4328 6058 2787 Size: 1541 1730 3168 Fragments arranged by size: 3168 1730 1541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2306 2588 3405 3501 3794 844 Size: 1462 282 817 96 293 3489 Fragments arranged by size: 3489 1462 817 293 282 96 BsrBI CCG'CTC Cuts at: 1394 1823 3103 4326 1394 Size: 429 1280 1223 3507 Fragments arranged by size: 3507 1280 1223 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2601 2634 2745 4934 5108 65 Size: 1183 1353 33 111 2189 174 1396 Fragments arranged by size: 2189 1396 1353 1183 174 111 33 BsrFI r'CCGG_y Cuts at: 1700 1810 5083 5270 1700 Size: 110 3273 187 2869 Fragments arranged by size: 3273 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6439 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6439 BssSI C'ACGA_G Cuts at: 2059 4499 5511 2059 Size: 2440 1012 2987 Fragments arranged by size: 2987 2440 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2476 3575 794 Size: 1682 1099 3658 Fragments arranged by size: 3658 1682 1099 BstBI TT'CG_AA Cuts at: 2007 5243 2007 Size: 3236 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6439 BstXI CCAn_nnnn'nTGG Cuts at: 3094 3416 3094 Size: 322 6117 BstYI r'GATC_y Cuts at: 1972 2571 2995 3192 3259 3769 4136 4521 4538 Size: 599 424 197 67 510 367 385 17 Cuts at: 4538 5990 6076 6088 6434 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 599 510 424 385 367 346 197 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 3078 3078 Size: 6439 BtgI C'CryG_G Cuts at: 513 1065 2051 513 Size: 552 986 4901 Fragments arranged by size: 4901 986 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5700 Fragments arranged by size: 5700 683 56 BtsI GCAGTG_nn' Cuts at: 2859 3019 3033 3955 4749 4769 2859 Size: 160 14 922 794 20 4529 Fragments arranged by size: 4529 922 794 160 20 14 ClaI AT'CG_AT Cuts at: 1554 2232 1554 Size: 678 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6404 DraI TTT'AAA Cuts at: 3743 3743 Size: 6439 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5446 809 Size: 507 4130 1802 Fragments arranged by size: 4130 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2985 3132 3418 Size: 54 1425 50 275 18 1155 147 286 Cuts at: 3418 3596 3706 3886 4775 6404 8 Size: 178 110 180 889 1629 43 Fragments arranged by size: 1629 1425 1155 889 286 275 180 178 147 110 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3596 3706 3886 1537 Size: 275 1784 110 180 4090 Fragments arranged by size: 4090 1784 275 180 110 EarI CTCTTCn'nnn_ Cuts at: 1353 2405 2703 4369 1353 Size: 1052 298 1666 3423 Fragments arranged by size: 3423 1666 1052 298 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5026 5400 5546 387 Size: 1064 138 3437 374 146 1280 Fragments arranged by size: 3437 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2563 2563 Size: 6439 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2244 3013 4502 5099 5886 6154 Size: 393 567 45 769 1489 597 787 268 Cuts at: 6154 1239 Size: 1524 Fragments arranged by size: 1524 1489 787 769 597 567 393 268 45 EcoNI CCTnn'n_nnAGG Cuts at: 2078 2372 2489 2078 Size: 294 117 6028 Fragments arranged by size: 6028 294 117 EcoRI G'AATT_C Cuts at: 3748 4126 3748 Size: 378 6061 EcoRV GAT'ATC Cuts at: 2632 2632 Size: 6439 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2326 3079 2326 Size: 753 5686 FspI TGC'GCA Cuts at: 4945 4945 Size: 6439 HaeII r_GCGC'y Cuts at: 1678 3698 5586 1678 Size: 2020 1888 2531 Fragments arranged by size: 2531 2020 1888 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2053 2076 2085 2093 2108 Size: 32 135 32 481 23 9 8 15 Cuts at: 2108 2125 2345 2377 2798 2830 3092 3124 5080 Size: 17 220 32 421 32 262 32 1956 Cuts at: 5080 5112 5154 5186 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1956 481 421 262 220 135 42 32 32 32 32 32 32 32 23 17 15 9 8 HincII GTy'rAC Cuts at: 669 1657 1938 2506 3783 4029 669 Size: 988 281 568 1277 246 3079 Fragments arranged by size: 3079 1277 988 568 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6439 HpaI GTT'AAC Cuts at: 2506 4029 2506 Size: 1523 4916 KasI G'GCGC_C Cuts at: 3694 3694 Size: 6439 KpnI G_GTAC'C Cuts at: 3764 3764 Size: 6439 MfeI C'AATT_G Cuts at: 2761 3048 4038 2761 Size: 287 990 5162 Fragments arranged by size: 5162 990 287 MluI A'CGCG_T Cuts at: 6315 6315 Size: 6439 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2640 3471 5553 5737 1305 Size: 1335 831 2082 184 2007 Fragments arranged by size: 2082 2007 1335 831 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2987 3134 10 Size: 54 1425 343 1155 147 3315 Fragments arranged by size: 3315 1425 1155 343 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2327 2454 3653 4397 4756 4915 518 Size: 648 1161 127 1199 744 359 159 2042 Fragments arranged by size: 2042 1199 1161 744 648 359 159 127 MspA1I CmG'CkG Cuts at: 1606 2053 2804 2991 3855 4532 5680 5925 1606 Size: 447 751 187 864 677 1148 245 2120 Fragments arranged by size: 2120 1148 864 751 677 447 245 187 NarI GG'CG_CC Cuts at: 3695 3695 Size: 6439 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5887 NdeI CA'TA_TG Cuts at: 387 387 Size: 6439 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6439 NotI GC'GGCC_GC Cuts at: 3886 3886 Size: 6439 NspI r_CATG'y Cuts at: 1132 1229 2438 2659 3500 3517 3797 5342 1132 Size: 97 1209 221 841 17 280 1545 2229 Fragments arranged by size: 2229 1545 1209 841 280 221 97 17 PasI CC'CwG_GG Cuts at: 1348 3409 1348 Size: 2061 4378 PciI A'CATG_T Cuts at: 5338 5338 Size: 6439 PflMI CCAn_nnn'nTGG Cuts at: 1108 3163 1108 Size: 2055 4384 PfoI T'CCnGG_A Cuts at: 2176 2176 Size: 6439 PmeI GTTT'AAAC Cuts at: 3743 3743 Size: 6439 PmlI CAC'GTG Cuts at: 3403 3403 Size: 6439 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2714 2746 4508 4540 6021 6053 2714 Size: 32 1762 32 1481 32 3100 Fragments arranged by size: 3100 1762 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6439 PsiI TTA'TAA Cuts at: 2532 4009 2532 Size: 1477 4962 PstI C_TGCA'G Cuts at: 830 3003 3437 3791 830 Size: 2173 434 354 3478 Fragments arranged by size: 3478 2173 434 354 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6439 PvuII CAG'CTG Cuts at: 2804 2804 Size: 6439 SacI G_AGCT'C Cuts at: 2565 2565 Size: 6439 SacII CC_GC'GG Cuts at: 2054 2054 Size: 6439 SalI G'TCGA_C Cuts at: 1936 3781 1936 Size: 1845 4594 SapI GCTCTTCn'nnn_ Cuts at: 2703 2703 Size: 6439 SbfI CC_TGCA'GG Cuts at: 3791 3791 Size: 6439 ScaI AGT'ACT Cuts at: 4687 6399 4687 Size: 1712 4727 SfcI C'TryA_G Cuts at: 826 1045 2999 3433 3787 4922 5603 5794 826 Size: 219 1954 434 354 1135 681 191 1471 Fragments arranged by size: 1954 1471 1135 681 434 354 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2685 2685 Size: 6439 SfoI GGC'GCC Cuts at: 3696 3696 Size: 6439 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6439 SmaI CCC'GGG Cuts at: 1872 3766 1872 Size: 1894 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2607 4543 5444 5706 820 Size: 197 926 664 1936 901 262 1553 Fragments arranged by size: 1936 1553 926 901 664 262 197 SnaBI TAC'GTA Cuts at: 493 3736 4230 493 Size: 3243 494 2702 Fragments arranged by size: 3243 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6439 SphI G_CATG'C Cuts at: 1132 3500 3797 1132 Size: 2368 297 3774 Fragments arranged by size: 3774 2368 297 SspI AAT'ATT Cuts at: 5 52 4363 5 Size: 47 4311 2081 Fragments arranged by size: 4311 2081 47 StuI AGG'CCT Cuts at: 1517 2378 1517 Size: 861 5578 StyI C'CwwG_G Cuts at: 513 1065 2032 2293 2910 3506 3878 513 Size: 552 967 261 617 596 372 3074 Fragments arranged by size: 3074 967 617 596 552 372 261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2462 3665 4466 4483 4636 4821 5266 Size: 1255 882 1203 801 17 153 185 445 Cuts at: 5266 325 Size: 1498 Fragments arranged by size: 1498 1255 1203 882 801 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2692 3312 4685 Size: 274 80 33 51 1443 715 620 1373 Cuts at: 4685 6397 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1373 715 620 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2047 2079 3058 3090 6399 6431 2047 Size: 32 979 32 3309 32 2055 Fragments arranged by size: 3309 2055 979 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2462 3248 3452 1316 Size: 1146 786 204 4303 Fragments arranged by size: 4303 1146 786 204 XbaI T'CTAG_A Cuts at: 3775 3775 Size: 6439 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2209 3047 3142 2209 Size: 838 95 5506 Fragments arranged by size: 5506 838 95 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6439 XmaI C'CCGG_G Cuts at: 1870 3764 1870 Size: 1894 4545 XmnI GAAnn'nnTTC Cuts at: 4568 4568 Size: 6439 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4247 276 Size: 53 83 186 936 2713 2468 Fragments arranged by size: 2713 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SanDI SexAI SrfI SwaI