(Circular) (Six-base) MAPSORT of: pFN21ASDB0027.seq Check: 3910 from: 1 to: 7666 pFN21ASDB0027 7666 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3444 5476 278 Size: 53 83 186 936 1908 2032 2468 Fragments arranged by size: 2468 2032 1908 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4113 4959 5009 1937 Size: 2176 846 50 4594 Fragments arranged by size: 4594 2176 846 50 Acc65I G'GTAC_C Cuts at: 4987 4987 Size: 7666 AclI AA'CG_TT Cuts at: 1563 3105 5793 6166 1563 Size: 1542 2688 373 3063 Fragments arranged by size: 3063 2688 1542 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5729 7113 7381 1632 Size: 4097 1384 268 1917 Fragments arranged by size: 4097 1917 1384 268 AfeI AGC'GCT Cuts at: 2260 4121 2260 Size: 1861 5805 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7469 AflIII A'CryG_T Cuts at: 1203 4629 4842 6565 7542 1203 Size: 3426 213 1723 977 1327 Fragments arranged by size: 3426 1723 1327 977 213 AgeI A'CCGG_T Cuts at: 6497 6497 Size: 7666 AhdI GACnn_n'nnGTC Cuts at: 2359 6395 2359 Size: 4036 3630 AleI CACnn'nnGTG Cuts at: 1166 4133 1166 Size: 2967 4699 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2970 3563 1184 Size: 1786 593 5287 Fragments arranged by size: 5287 1786 593 AlwNI CAG_nnn'CTG Cuts at: 2326 2338 2917 3431 3628 3982 5409 6981 2326 Size: 12 579 514 197 354 1427 1572 3011 Fragments arranged by size: 3011 1572 1427 579 514 354 197 12 ApaI G_GGCC'C Cuts at: 2041 2911 2041 Size: 870 6796 ApaLI G'TGCA_C Cuts at: 4681 5723 6879 4681 Size: 1042 1156 5468 Fragments arranged by size: 5468 1156 1042 ApoI r'AATT_y Cuts at: 1493 3409 4975 5201 5353 7462 1493 Size: 1916 1566 226 152 2109 1697 Fragments arranged by size: 2109 1916 1697 1566 226 152 AseI AT'TA_AT Cuts at: 160 1032 6220 160 Size: 872 5188 1606 Fragments arranged by size: 5188 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7666 AvaI C'yCGr_G Cuts at: 1870 1943 2089 2672 2978 3515 4762 4991 1870 Size: 73 146 583 306 537 1247 229 4545 Fragments arranged by size: 4545 1247 583 537 306 229 146 73 BamHI G'GATC_C Cuts at: 2028 4996 5363 2028 Size: 2968 367 4331 Fragments arranged by size: 4331 2968 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3554 3586 3835 3926 4430 Size: 325 217 627 1767 32 249 91 504 Cuts at: 4430 4648 4987 6442 618 Size: 218 339 1455 1842 Fragments arranged by size: 1842 1767 1455 627 504 339 325 249 218 217 91 32 BanII G_rGCy'C Cuts at: 1391 2041 2096 2224 2247 2746 2911 4017 4110 Size: 650 55 128 23 499 165 1106 93 Cuts at: 4110 4349 4823 4985 1391 Size: 239 474 162 4072 Fragments arranged by size: 4072 1106 650 499 474 239 165 162 128 93 55 23 BbeI G_GCGC'C Cuts at: 3558 3558 Size: 7666 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2769 3448 4032 4148 928 Size: 971 870 679 584 116 4446 Fragments arranged by size: 4446 971 870 679 584 116 BbvCI CC'TCA_GC Cuts at: 3564 3845 3564 Size: 281 7385 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4732 4766 5857 5891 1903 Size: 34 2795 34 1091 34 3678 Fragments arranged by size: 3678 2795 1091 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5558 6768 5558 Size: 1210 6456 BclI T'GATC_A Cuts at: 1548 1548 Size: 7666 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2210 2219 3116 Size: 107 122 71 631 605 538 9 897 Cuts at: 3116 4478 4623 6277 136 Size: 1362 145 1654 1525 Fragments arranged by size: 1654 1525 1362 897 631 605 538 145 122 107 71 9 BglII A'GATC_T Cuts at: 2754 7661 2754 Size: 4907 2759 BlpI GC'TnA_GC Cuts at: 3345 3424 3674 4232 5083 3345 Size: 79 250 558 851 5928 Fragments arranged by size: 5928 851 558 250 79 Bme1580I G_kGCm'C Cuts at: 1790 2041 2911 3007 3591 3840 4433 4637 4685 Size: 251 870 96 584 249 593 204 48 Cuts at: 4685 5727 6883 1790 Size: 1042 1156 2573 Fragments arranged by size: 2573 1156 1042 870 593 584 251 249 204 96 48 BmgBI CAC'GTC Cuts at: 1318 4686 1318 Size: 3368 4298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3700 5397 6355 Size: 416 53 83 399 24 2283 1697 958 Cuts at: 6355 442 Size: 1753 Fragments arranged by size: 2283 1753 1697 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7666 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4471 4738 4471 Size: 267 7399 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3830 4070 6326 1239 Size: 2591 240 2256 2579 Fragments arranged by size: 2591 2579 2256 240 Bpu10I CC'TnA_GC Cuts at: 3564 3600 3845 4152 4179 3564 Size: 36 245 307 27 7051 Fragments arranged by size: 7051 307 245 36 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2447 2492 3134 3230 3707 4001 4913 5791 6656 Size: 45 642 96 477 294 912 878 865 Cuts at: 6656 6954 2447 Size: 298 3159 Fragments arranged by size: 3159 912 878 865 642 477 298 294 96 45 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2238 2245 4359 6329 882 Size: 617 739 7 2114 1970 2219 Fragments arranged by size: 2219 2114 1970 739 617 7 BsaAI yAC'GTr Cuts at: 493 1204 4264 4630 4963 5457 493 Size: 711 3060 366 333 494 2702 Fragments arranged by size: 3060 2702 711 494 366 333 BsaBI GATnn'nnATC Cuts at: 3197 7660 3197 Size: 4463 3203 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2790 3441 3555 3918 Size: 53 83 186 936 1257 651 114 363 Cuts at: 3918 5473 5855 6490 275 Size: 1555 382 635 1451 Fragments arranged by size: 1555 1451 1257 936 651 635 382 363 186 114 83 53 BsaWI w'CCGG_w Cuts at: 2862 6099 6497 6771 6918 7329 2862 Size: 3237 398 274 147 411 3199 Fragments arranged by size: 3237 3199 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4043 4073 1178 Size: 30 165 30 2640 30 4771 Fragments arranged by size: 4771 2640 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2590 3275 3494 4727 1186 Size: 1404 685 219 1233 4125 Fragments arranged by size: 4125 1404 1233 685 219 BseYI C'CCAG_C Cuts at: 2255 2685 3550 4170 6869 7365 2255 Size: 430 865 620 2699 496 2556 Fragments arranged by size: 2699 2556 865 620 496 430 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2411 3417 3808 4157 4550 4796 1163 Size: 573 675 1006 391 349 393 246 4033 Fragments arranged by size: 4033 1006 675 573 393 391 349 246 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2110 4618 5116 5877 6905 1540 Size: 275 186 109 2508 498 761 1028 2301 Fragments arranged by size: 2508 2301 1028 761 498 275 186 109 BsiHKAI G_wGCw'C Cuts at: 2949 3425 4017 4349 4685 5727 5812 6883 2949 Size: 476 592 332 336 1042 85 1071 3732 Fragments arranged by size: 3732 1071 1042 592 476 336 332 85 BsmI GAATG_Cn' Cuts at: 2625 5177 5270 2625 Size: 2552 93 5021 Fragments arranged by size: 5021 2552 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2096 2224 2247 2746 2911 2949 Size: 399 251 55 128 23 499 165 38 Cuts at: 2949 3007 3425 3591 3840 4017 4110 4349 4433 Size: 58 418 166 249 177 93 239 84 Cuts at: 4433 4637 4685 4823 4985 5727 5812 6883 1391 Size: 204 48 138 162 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 499 418 399 251 249 239 204 177 166 165 162 138 128 93 85 84 58 55 48 38 23 BspEI T'CCGG_A Cuts at: 7329 7329 Size: 7666 BspHI T'CATG_A Cuts at: 2814 4209 4293 5555 7285 2814 Size: 1395 84 1262 1730 3195 Fragments arranged by size: 3195 1730 1395 1262 84 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2323 2706 3603 3909 5021 844 Size: 1479 383 897 306 1112 3489 Fragments arranged by size: 3489 1479 1112 897 383 306 BsrBI CCG'CTC Cuts at: 1394 1823 3349 3654 5553 1394 Size: 429 1526 305 1899 3507 Fragments arranged by size: 3507 1899 1526 429 305 BsrDI GCAATG_nn' Cuts at: 65 1248 3558 4298 6161 6335 65 Size: 1183 2310 740 1863 174 1396 Fragments arranged by size: 2310 1863 1396 1183 740 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6310 6497 1700 Size: 110 4500 187 2869 Fragments arranged by size: 4500 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4164 96 Size: 4068 3598 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7666 BssSI C'ACGA_G Cuts at: 3338 5726 6738 3338 Size: 2388 1012 4266 Fragments arranged by size: 4266 2388 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2326 2338 794 Size: 1532 12 6122 Fragments arranged by size: 6122 1532 12 BstBI TT'CG_AA Cuts at: 2007 6470 2007 Size: 4463 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7666 BstYI r'GATC_y Cuts at: 1972 2028 2754 2859 4225 4996 5363 5748 5765 Size: 56 726 105 1366 771 367 385 17 Cuts at: 5765 7217 7303 7315 7661 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1366 771 726 385 367 346 105 86 56 17 12 Bsu36I CC'TnA_GG Cuts at: 2215 4332 4691 2215 Size: 2117 359 5190 Fragments arranged by size: 5190 2117 359 BtgI C'CryG_G Cuts at: 513 1065 2208 2298 4732 513 Size: 552 1143 90 2434 3447 Fragments arranged by size: 3447 2434 1143 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2607 2626 2860 3643 4812 487 Size: 683 56 1381 19 234 783 1169 3341 Fragments arranged by size: 3341 1381 1169 783 683 234 56 19 BtsI GCAGTG_nn' Cuts at: 2845 3329 3799 4586 5182 5976 5996 2845 Size: 484 470 787 596 794 20 4515 Fragments arranged by size: 4515 794 787 596 484 470 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7666 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7631 DraI TTT'AAA Cuts at: 4970 4970 Size: 7666 DraIII CAC_nnn'GTG Cuts at: 3694 3694 Size: 7666 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3866 6673 809 Size: 507 2550 2807 1802 Fragments arranged by size: 2807 2550 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 2202 3141 Size: 54 1425 50 275 18 277 95 939 Cuts at: 3141 3933 4068 4242 4615 5113 6002 7631 8 Size: 792 135 174 373 498 889 1629 43 Fragments arranged by size: 1629 1425 939 889 792 498 373 277 275 174 135 95 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2107 4615 5113 1537 Size: 275 295 2508 498 4090 Fragments arranged by size: 4090 2508 498 295 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3512 3810 4043 5596 1353 Size: 2159 298 233 1553 3423 Fragments arranged by size: 3423 2159 1553 298 233 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2021 2157 2165 6253 6627 6773 Size: 1064 138 432 136 8 4088 374 146 Cuts at: 6773 387 Size: 1280 Fragments arranged by size: 4088 1280 1064 432 374 146 138 136 8 EcoICRI GAG'CTC Cuts at: 4015 4347 4015 Size: 332 7334 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3830 4070 5729 6326 7113 7381 1239 Size: 393 2198 240 1659 597 787 268 1524 Fragments arranged by size: 2198 1659 1524 787 597 393 268 240 EcoNI CCTnn'n_nnAGG Cuts at: 2330 3245 4244 2330 Size: 915 999 5752 Fragments arranged by size: 5752 999 915 EcoO109I rG'GnC_Cy Cuts at: 2038 2132 2323 2335 2802 3084 4033 4249 2038 Size: 94 191 12 467 282 949 216 5455 Fragments arranged by size: 5455 949 467 282 216 191 94 12 EcoRI G'AATT_C Cuts at: 4975 5353 4975 Size: 378 7288 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3322 3661 4054 3322 Size: 339 393 6934 Fragments arranged by size: 6934 393 339 FspI TGC'GCA Cuts at: 6172 6172 Size: 7666 HaeII r_GCGC'y Cuts at: 1678 2262 2918 3558 4123 6813 1678 Size: 584 656 640 565 2690 2531 Fragments arranged by size: 2690 2531 656 640 584 565 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2585 2617 3383 Size: 32 135 32 773 32 208 32 766 Cuts at: 3383 3415 4043 4075 4135 4167 6307 6339 6381 Size: 32 628 32 60 32 2140 32 42 Cuts at: 6381 6413 1373 Size: 32 2626 Fragments arranged by size: 2626 2140 773 766 628 208 135 60 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4759 5010 5256 669 Size: 988 281 2821 251 246 3079 Fragments arranged by size: 3079 2821 988 281 251 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7666 HpaI GTT'AAC Cuts at: 5256 5256 Size: 7666 KasI G'GCGC_C Cuts at: 3554 3554 Size: 7666 KpnI G_GTAC'C Cuts at: 4991 4991 Size: 7666 MfeI C'AATT_G Cuts at: 5265 5265 Size: 7666 MluI A'CGCG_T Cuts at: 7542 7542 Size: 7666 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3426 3612 6780 6964 1305 Size: 2121 186 3168 184 2007 Fragments arranged by size: 3168 2121 2007 186 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4070 10 Size: 54 1425 343 2238 3606 Fragments arranged by size: 3606 2238 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3203 3545 4133 4502 4742 5624 5983 Size: 648 2037 342 588 369 240 882 359 Cuts at: 5983 6142 518 Size: 159 2042 Fragments arranged by size: 2042 2037 882 648 588 369 359 342 240 159 MspA1I CmG'CkG Cuts at: 1606 2124 2210 2344 3428 4236 4273 4734 4924 Size: 518 86 134 1084 808 37 461 190 Cuts at: 4924 5082 5759 6907 7152 1606 Size: 158 677 1148 245 2120 Fragments arranged by size: 2120 1148 1084 808 677 518 461 245 190 158 134 86 37 NarI GG'CG_CC Cuts at: 3555 3555 Size: 7666 NcoI C'CATG_G Cuts at: 513 1065 2298 513 Size: 552 1233 5881 Fragments arranged by size: 5881 1233 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7666 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7666 NotI GC'GGCC_GC Cuts at: 5113 5113 Size: 7666 NspI r_CATG'y Cuts at: 1132 1229 3055 3076 3577 3718 4318 4501 4846 Size: 97 1826 21 501 141 600 183 345 Cuts at: 4846 5024 6569 1132 Size: 178 1545 2229 Fragments arranged by size: 2229 1826 1545 600 501 345 183 178 141 97 21 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7666 PciI A'CATG_T Cuts at: 4842 6565 4842 Size: 1723 5943 PflMI CCAn_nnn'nTGG Cuts at: 1108 2304 3694 4645 1108 Size: 1196 1390 951 4129 Fragments arranged by size: 4129 1390 1196 951 PfoI T'CCnGG_A Cuts at: 3459 3756 3855 3459 Size: 297 99 7270 Fragments arranged by size: 7270 297 99 PmeI GTTT'AAAC Cuts at: 4970 4970 Size: 7666 PmlI CAC'GTG Cuts at: 4264 4264 Size: 7666 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5735 5767 7248 7280 5735 Size: 32 1481 32 6121 Fragments arranged by size: 6121 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2132 2335 3084 4033 2132 Size: 203 749 949 5765 Fragments arranged by size: 5765 949 749 203 PshAI GACnn'nnGTC Cuts at: 1613 2464 1613 Size: 851 6815 PsiI TTA'TAA Cuts at: 4851 5236 4851 Size: 385 7281 PspOMI G'GGCC_C Cuts at: 2037 2907 2037 Size: 870 6796 PstI C_TGCA'G Cuts at: 830 2331 2343 2664 2751 2835 2852 3081 3794 Size: 1501 12 321 87 84 17 229 713 Cuts at: 3794 4083 4662 5018 830 Size: 289 579 356 3478 Fragments arranged by size: 3478 1501 713 579 356 321 289 229 87 84 17 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7666 PvuII CAG'CTG Cuts at: 2124 2344 4273 2124 Size: 220 1929 5517 Fragments arranged by size: 5517 1929 220 SacI G_AGCT'C Cuts at: 4017 4349 4017 Size: 332 7334 SacII CC_GC'GG Cuts at: 2211 4735 2211 Size: 2524 5142 SalI G'TCGA_C Cuts at: 1936 5008 1936 Size: 3072 4594 SanDI GG'GwC_CC Cuts at: 2132 2132 Size: 7666 SbfI CC_TGCA'GG Cuts at: 5018 5018 Size: 7666 ScaI AGT'ACT Cuts at: 3221 5914 7626 3221 Size: 2693 1712 3261 Fragments arranged by size: 3261 2693 1712 SexAI A'CCwGG_T Cuts at: 4548 4548 Size: 7666 SfcI C'TryA_G Cuts at: 826 1045 2327 2339 2660 2747 2831 2848 3077 Size: 219 1282 12 321 87 84 17 229 Cuts at: 3077 3790 3942 4079 4658 4803 5014 6149 6830 Size: 713 152 137 579 145 211 1135 681 Cuts at: 6830 7021 826 Size: 191 1471 Fragments arranged by size: 1471 1282 1135 713 681 579 321 229 219 211 191 152 145 137 87 84 17 12 SfiI GGCCn_nnn'nGGCC Cuts at: 2210 2210 Size: 7666 SfoI GGC'GCC Cuts at: 3556 3556 Size: 7666 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7666 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7666 SmaI CCC'GGG Cuts at: 1872 4764 4993 1872 Size: 2892 229 4545 Fragments arranged by size: 4545 2892 229 SmlI C'TyrA_G Cuts at: 820 1017 1943 2462 2507 3113 3245 3722 4016 Size: 197 926 519 45 606 132 477 294 Cuts at: 4016 4928 5770 6671 6933 820 Size: 912 842 901 262 1553 Fragments arranged by size: 1553 926 912 901 842 606 519 477 294 262 197 132 45 SnaBI TAC'GTA Cuts at: 493 4963 5457 493 Size: 4470 494 2702 Fragments arranged by size: 4470 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7666 SphI G_CATG'C Cuts at: 1132 3577 4318 4501 5024 1132 Size: 2445 741 183 523 3774 Fragments arranged by size: 3774 2445 741 523 183 SspI AAT'ATT Cuts at: 5 52 2929 5590 5 Size: 47 2877 2661 2081 Fragments arranged by size: 2877 2661 2081 47 StuI AGG'CCT Cuts at: 1517 2164 3119 3501 3644 3821 1517 Size: 647 955 382 143 177 5362 Fragments arranged by size: 5362 955 647 382 177 143 StyI C'CwwG_G Cuts at: 513 1065 2298 2649 3645 4676 5105 513 Size: 552 1233 351 996 1031 429 3074 Fragments arranged by size: 3074 1233 1031 996 552 429 351 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3511 3682 4184 4244 4594 4666 4804 Size: 1255 1931 171 502 60 350 72 138 Cuts at: 4804 5693 5710 5863 6048 6493 325 Size: 889 17 153 185 445 1498 Fragments arranged by size: 1931 1498 1255 889 502 445 350 185 171 153 138 72 60 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3069 3219 4164 Size: 274 80 33 51 1443 1092 150 945 Cuts at: 4164 5912 7624 96 Size: 1748 1712 138 Fragments arranged by size: 1748 1712 1443 1092 945 274 150 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3426 3458 7626 7658 3426 Size: 32 4168 32 3434 Fragments arranged by size: 4168 3434 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7666 XbaI T'CTAG_A Cuts at: 5002 5002 Size: 7666 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3928 4588 4618 3928 Size: 660 30 6976 Fragments arranged by size: 6976 660 30 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7666 XmaI C'CCGG_G Cuts at: 1870 4762 4991 1870 Size: 2892 229 4545 Fragments arranged by size: 4545 2892 229 XmnI GAAnn'nnTTC Cuts at: 2891 5795 2891 Size: 2904 4762 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3442 5474 276 Size: 53 83 186 936 1908 2032 2468 Fragments arranged by size: 2468 2032 1908 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SapI SrfI SwaI