(Circular) (Six-base) MAPSORT of: pFN21ASDB0024.seq Check: 1097 from: 1 to: 8470 pFN21ASDB0024 8470 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6280 278 Size: 53 83 186 936 4744 2468 Fragments arranged by size: 4744 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5754 5813 1937 Size: 3817 59 4594 Fragments arranged by size: 4594 3817 59 Acc65I G'GTAC_C Cuts at: 4124 5791 4124 Size: 1667 6803 AclI AA'CG_TT Cuts at: 1563 2890 4959 6597 6970 1563 Size: 1327 2069 1638 373 3063 Fragments arranged by size: 3063 2069 1638 1327 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 3264 3312 3965 4077 4608 4908 4935 Size: 563 1069 48 653 112 531 300 27 Cuts at: 4935 5578 6533 7917 8185 1632 Size: 643 955 1384 268 1917 Fragments arranged by size: 1917 1384 1069 955 653 643 563 531 300 268 112 48 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8273 AflIII A'CryG_T Cuts at: 1203 5334 7369 8346 1203 Size: 4131 2035 977 1327 Fragments arranged by size: 4131 2035 1327 977 AgeI A'CCGG_T Cuts at: 7301 7301 Size: 8470 AhdI GACnn_n'nnGTC Cuts at: 2771 3718 7199 2771 Size: 947 3481 4042 Fragments arranged by size: 4042 3481 947 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8470 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3791 4883 1184 Size: 2607 1092 4771 Fragments arranged by size: 4771 2607 1092 AlwNI CAG_nnn'CTG Cuts at: 5062 6213 7785 5062 Size: 1151 1572 5747 Fragments arranged by size: 5747 1572 1151 ApaI G_GGCC'C Cuts at: 5038 5038 Size: 8470 ApaLI G'TGCA_C Cuts at: 6527 7683 6527 Size: 1156 7314 ApoI r'AATT_y Cuts at: 1493 2327 4102 5779 6005 6157 8266 1493 Size: 834 1775 1677 226 152 2109 1697 Fragments arranged by size: 2109 1775 1697 1677 834 226 152 AseI AT'TA_AT Cuts at: 160 1032 2049 7024 160 Size: 872 1017 4975 1606 Fragments arranged by size: 4975 1606 1017 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8470 AvaI C'yCGr_G Cuts at: 1870 1943 2910 4448 5795 1870 Size: 73 967 1538 1347 4545 Fragments arranged by size: 4545 1538 1347 967 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4108 4141 4108 Size: 33 8437 BamHI G'GATC_C Cuts at: 2726 3701 5800 6167 2726 Size: 975 2099 367 5029 Fragments arranged by size: 5029 2099 975 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4124 4805 5791 7246 618 Size: 325 217 627 2337 681 986 1455 1842 Fragments arranged by size: 2337 1842 1455 986 681 627 325 217 BanII G_rGCy'C Cuts at: 1391 4366 5038 5789 1391 Size: 2975 672 751 4072 Fragments arranged by size: 4072 2975 751 672 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2147 3757 4509 4657 5308 928 Size: 971 248 1610 752 148 651 4090 Fragments arranged by size: 4090 1610 971 752 651 248 148 BbvCI CC'TCA_GC Cuts at: 3676 3676 Size: 8470 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6661 6695 1903 Size: 34 4724 34 3678 Fragments arranged by size: 4724 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2533 3415 5364 6362 7572 2533 Size: 882 1949 998 1210 3431 Fragments arranged by size: 3431 1949 1210 998 882 BclI T'GATC_A Cuts at: 1548 1548 Size: 8470 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4095 7081 136 Size: 107 122 71 631 605 2423 2986 1525 Fragments arranged by size: 2986 2423 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3599 5194 8465 3599 Size: 1595 3271 3604 Fragments arranged by size: 3604 3271 1595 BlpI GC'TnA_GC Cuts at: 5470 5887 5470 Size: 417 8053 Bme1580I G_kGCm'C Cuts at: 1790 5038 6531 7687 1790 Size: 3248 1493 1156 2573 Fragments arranged by size: 3248 2573 1493 1156 BmgBI CAC'GTC Cuts at: 1318 5335 5600 1318 Size: 4017 265 4188 Fragments arranged by size: 4188 4017 265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2500 4173 4705 Size: 416 53 83 399 24 1083 1673 532 Cuts at: 4705 5032 6201 7159 442 Size: 327 1169 958 1753 Fragments arranged by size: 1753 1673 1169 1083 958 532 416 399 327 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3197 3971 1056 Size: 2141 774 5555 Fragments arranged by size: 5555 2141 774 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2255 2255 Size: 8470 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2456 4983 5632 7130 1239 Size: 986 231 2527 649 1498 2579 Fragments arranged by size: 2579 2527 1498 986 649 231 Bpu10I CC'TnA_GC Cuts at: 3676 4599 3676 Size: 923 7547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2528 3209 4454 4617 5432 6595 7460 7758 2528 Size: 681 1245 163 815 1163 865 298 3240 Fragments arranged by size: 3240 1245 1163 865 815 681 298 163 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2168 7133 882 Size: 617 669 4965 2219 Fragments arranged by size: 4965 2219 669 617 BsaAI yAC'GTr Cuts at: 493 1204 4774 5767 6261 493 Size: 711 3570 993 494 2702 Fragments arranged by size: 3570 2702 993 711 494 BsaBI GATnn'nnATC Cuts at: 5079 8464 5079 Size: 3385 5085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5742 6277 6659 7294 Size: 53 83 186 936 4209 535 382 635 Cuts at: 7294 275 Size: 1451 Fragments arranged by size: 4209 1451 936 635 535 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2323 5044 6903 7301 7575 7722 8133 2323 Size: 2721 1859 398 274 147 411 2660 Fragments arranged by size: 2721 2660 1859 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 4330 4360 1178 Size: 30 165 30 687 30 2210 30 5288 Fragments arranged by size: 5288 2210 687 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3413 4335 4338 4920 5619 1186 Size: 2227 922 3 582 699 4037 Fragments arranged by size: 4037 2227 922 699 582 3 BseYI C'CCAG_C Cuts at: 2199 5031 5111 7673 8169 2199 Size: 2832 80 2562 496 2500 Fragments arranged by size: 2832 2562 2500 496 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2319 2616 1163 Size: 573 583 297 7017 Fragments arranged by size: 7017 583 573 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5920 6681 7709 1540 Size: 275 186 3919 761 1028 2301 Fragments arranged by size: 3919 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2571 3073 6531 6616 7687 2571 Size: 502 3458 85 1071 3354 Fragments arranged by size: 3458 3354 1071 502 85 BsmI GAATG_Cn' Cuts at: 4764 5156 5981 6074 4764 Size: 392 825 93 7160 Fragments arranged by size: 7160 825 392 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2571 3073 4366 5038 5789 6531 6616 Size: 399 781 502 1293 672 751 742 85 Cuts at: 6616 7687 1391 Size: 1071 2174 Fragments arranged by size: 2174 1293 1071 781 751 742 672 502 399 85 BspEI T'CCGG_A Cuts at: 8133 8133 Size: 8470 BspHI T'CATG_A Cuts at: 4245 4972 5083 6359 8089 4245 Size: 727 111 1276 1730 4626 Fragments arranged by size: 4626 1730 1276 727 111 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5825 844 Size: 4981 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5418 6357 1394 Size: 429 3595 939 3507 Fragments arranged by size: 3595 3507 939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5356 6965 7139 65 Size: 1183 4108 1609 174 1396 Fragments arranged by size: 4108 1609 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7114 7301 1700 Size: 110 5304 187 2869 Fragments arranged by size: 5304 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8470 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8470 BssSI C'ACGA_G Cuts at: 3815 5631 6530 7542 3815 Size: 1816 899 1012 4743 Fragments arranged by size: 4743 1816 1012 899 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8470 BstBI TT'CG_AA Cuts at: 2007 7274 2007 Size: 5267 3203 BstEII G'GTnAC_C Cuts at: 1184 5121 1184 Size: 3937 4533 BstXI CCAn_nnnn'nTGG Cuts at: 2090 4228 4718 2090 Size: 2138 490 5842 Fragments arranged by size: 5842 2138 490 BstYI r'GATC_y Cuts at: 1972 2726 2953 3599 3701 3928 4836 5194 5800 Size: 754 227 646 102 227 908 358 606 Cuts at: 5800 6167 6552 6569 8021 8107 8119 8465 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 908 754 646 606 385 367 358 346 227 227 102 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2515 2515 Size: 8470 BtgI C'CryG_G Cuts at: 513 1065 3009 513 Size: 552 1944 5974 Fragments arranged by size: 5974 1944 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4140 4856 487 Size: 683 56 2914 716 4101 Fragments arranged by size: 4101 2914 716 683 56 BtsI GCAGTG_nn' Cuts at: 2095 4250 4865 5986 6780 6800 2095 Size: 2155 615 1121 794 20 3765 Fragments arranged by size: 3765 2155 1121 794 615 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8470 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8435 DraI TTT'AAA Cuts at: 5211 5774 5211 Size: 563 7907 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7477 809 Size: 507 6161 1802 Fragments arranged by size: 6161 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4161 5917 6806 Size: 54 1425 50 275 18 2331 1756 889 Cuts at: 6806 8435 8 Size: 1629 43 Fragments arranged by size: 2331 1756 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5917 1537 Size: 275 4105 4090 Fragments arranged by size: 4105 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3153 3288 3342 3775 4488 4793 6400 1353 Size: 1800 135 54 433 713 305 1607 3423 Fragments arranged by size: 3423 1800 1607 713 433 305 135 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7057 7431 7577 387 Size: 1064 138 5468 374 146 1280 Fragments arranged by size: 5468 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2225 2456 3264 3312 3965 4077 Size: 393 563 30 231 808 48 653 112 Cuts at: 4077 4608 4908 4935 4983 5578 5632 6533 7130 Size: 531 300 27 48 595 54 901 597 Cuts at: 7130 7917 8185 1239 Size: 787 268 1524 Fragments arranged by size: 1524 901 808 787 653 597 595 563 531 393 300 268 231 112 54 48 48 30 27 EcoNI CCTnn'n_nnAGG Cuts at: 2267 3284 5614 2267 Size: 1017 2330 5123 Fragments arranged by size: 5123 2330 1017 EcoO109I rG'GnC_Cy Cuts at: 2079 5664 2079 Size: 3585 4885 EcoRI G'AATT_C Cuts at: 5779 6157 5779 Size: 378 8092 EcoRV GAT'ATC Cuts at: 2275 2275 Size: 8470 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2867 3203 4499 4574 2867 Size: 336 1296 75 6763 Fragments arranged by size: 6763 1296 336 75 FspI TGC'GCA Cuts at: 6976 6976 Size: 8470 HaeII r_GCGC'y Cuts at: 1678 7617 1678 Size: 5939 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 2219 2251 2264 Size: 32 135 32 518 32 97 32 13 Cuts at: 2264 2296 2757 2789 3704 3736 3920 3952 4258 Size: 32 461 32 915 32 184 32 306 Cuts at: 4258 4290 4366 4398 4878 4910 7111 7143 7185 Size: 32 76 32 480 32 2201 32 42 Cuts at: 7185 7217 1373 Size: 32 2626 Fragments arranged by size: 2626 2201 915 518 480 461 306 184 135 97 76 42 32 32 32 32 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 3341 5697 5814 6060 669 Size: 988 281 1403 2356 117 246 3079 Fragments arranged by size: 3079 2356 1403 988 281 246 117 HindIII A'AGCT_T Cuts at: 748 748 Size: 8470 HpaI GTT'AAC Cuts at: 5697 6060 5697 Size: 363 8107 KpnI G_GTAC'C Cuts at: 4128 5795 4128 Size: 1667 6803 MfeI C'AATT_G Cuts at: 3614 3989 5379 6069 3614 Size: 375 1390 690 6015 Fragments arranged by size: 6015 1390 690 375 MluI A'CGCG_T Cuts at: 8346 8346 Size: 8470 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4958 5516 7584 7768 1305 Size: 3653 558 2068 184 2007 Fragments arranged by size: 3653 2068 2007 558 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4163 10 Size: 54 1425 343 2331 4317 Fragments arranged by size: 4317 2331 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 3773 3878 3954 4404 6428 6787 Size: 648 897 1710 105 76 450 2024 359 Cuts at: 6787 6946 518 Size: 159 2042 Fragments arranged by size: 2042 2024 1710 897 648 450 359 159 105 76 MspA1I CmG'CkG Cuts at: 1606 2122 3680 5684 5886 6563 7711 7956 1606 Size: 516 1558 2004 202 677 1148 245 2120 Fragments arranged by size: 2120 2004 1558 1148 677 516 245 202 NcoI C'CATG_G Cuts at: 513 1065 3009 513 Size: 552 1944 5974 Fragments arranged by size: 5974 1944 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8470 NheI G'CTAG_C Cuts at: 1052 3193 3967 1052 Size: 2141 774 5555 Fragments arranged by size: 5555 2141 774 NotI GC'GGCC_GC Cuts at: 5917 5917 Size: 8470 NspI r_CATG'y Cuts at: 1132 1229 5828 7373 1132 Size: 97 4599 1545 2229 Fragments arranged by size: 4599 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4609 5551 1348 Size: 3261 942 4267 Fragments arranged by size: 4267 3261 942 PciI A'CATG_T Cuts at: 7369 7369 Size: 8470 PflMI CCAn_nnn'nTGG Cuts at: 1108 4903 1108 Size: 3795 4675 PfoI T'CCnGG_A Cuts at: 4896 4896 Size: 8470 PmeI GTTT'AAAC Cuts at: 5774 5774 Size: 8470 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6539 6571 8052 8084 6539 Size: 32 1481 32 6925 Fragments arranged by size: 6925 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2079 5664 2079 Size: 3585 4885 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8470 PsiI TTA'TAA Cuts at: 6040 6040 Size: 8470 PspOMI G'GGCC_C Cuts at: 5034 5034 Size: 8470 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3636 3668 3636 Size: 32 8438 PstI C_TGCA'G Cuts at: 830 2363 4597 5822 830 Size: 1533 2234 1225 3478 Fragments arranged by size: 3478 2234 1533 1225 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8470 PvuII CAG'CTG Cuts at: 2122 3680 5684 2122 Size: 1558 2004 4908 Fragments arranged by size: 4908 2004 1558 SalI G'TCGA_C Cuts at: 1936 5812 1936 Size: 3876 4594 SanDI GG'GwC_CC Cuts at: 2079 2079 Size: 8470 SbfI CC_TGCA'GG Cuts at: 2363 5822 2363 Size: 3459 5011 ScaI AGT'ACT Cuts at: 3846 6718 8430 3846 Size: 2872 1712 3886 Fragments arranged by size: 3886 2872 1712 SfcI C'TryA_G Cuts at: 826 1045 2359 2802 4041 4593 5054 5461 5818 Size: 219 1314 443 1239 552 461 407 357 Cuts at: 5818 6953 7634 7825 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1314 1239 1135 681 552 461 443 407 357 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8470 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8470 SmaI CCC'GGG Cuts at: 1872 5797 1872 Size: 3925 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2543 3188 4433 4632 5411 6574 Size: 197 926 600 645 1245 199 779 1163 Cuts at: 6574 7475 7737 820 Size: 901 262 1553 Fragments arranged by size: 1553 1245 1163 926 901 779 645 600 262 199 197 SnaBI TAC'GTA Cuts at: 493 5767 6261 493 Size: 5274 494 2702 Fragments arranged by size: 5274 2702 494 SpeI A'CTAG_T Cuts at: 152 2822 152 Size: 2670 5800 SphI G_CATG'C Cuts at: 1132 5828 1132 Size: 4696 3774 SspI AAT'ATT Cuts at: 5 52 3310 6394 5 Size: 47 3258 3084 2081 Fragments arranged by size: 3258 3084 2081 47 StuI AGG'CCT Cuts at: 1517 2579 4098 1517 Size: 1062 1519 5889 Fragments arranged by size: 5889 1519 1062 StyI C'CwwG_G Cuts at: 513 1065 2580 3009 3285 3755 5909 513 Size: 552 1515 429 276 470 2154 3074 Fragments arranged by size: 3074 2154 1515 552 470 429 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2244 3761 4134 4954 5370 5519 6497 Size: 1255 664 1517 373 820 416 149 978 Cuts at: 6497 6514 6667 6852 7297 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1517 1498 1255 978 820 664 445 416 373 185 153 149 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3844 5130 5638 Size: 274 80 33 51 1443 1867 1286 508 Cuts at: 5638 6716 8428 96 Size: 1078 1712 138 Fragments arranged by size: 1867 1712 1443 1286 1078 508 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3693 3725 3803 3835 8430 8462 3693 Size: 32 78 32 4595 32 3701 Fragments arranged by size: 4595 3701 78 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8470 XbaI T'CTAG_A Cuts at: 2158 2374 3493 3667 5806 2158 Size: 216 1119 174 2139 4822 Fragments arranged by size: 4822 2139 1119 216 174 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4847 4847 Size: 8470 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8470 XmaI C'CCGG_G Cuts at: 1870 5795 1870 Size: 3925 4545 XmnI GAAnn'nnTTC Cuts at: 2370 4948 5106 6599 2370 Size: 2578 158 1493 4241 Fragments arranged by size: 4241 2578 1493 158 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6278 276 Size: 53 83 186 936 4744 2468 Fragments arranged by size: 4744 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI