Restriction map for
pFN21ASDB0021
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
BamHI BbrPI BbvCI BciVI BclI
BfrI BlpI Bpu1102I BsaAI BsaBI
BsgI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I CelII
ClaI DraI DraIII DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI HincII
HindII HindIII HpaI MamI MfeI
MluI MseI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI PvuII
RcaI SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I TspRI Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 469 GGTACC G^GTACC
AccI 136 GTCTAC GT^[AC][GT]AC
AccIII 894 TCCGGA T^CCGGA
Asp718I 469 GGTACC G^GTACC
AspHI 960 GAGCAC G[AT]GC[AT]^C
AviII 119 TGCGCA TGC^GCA
AvrII 537 CCTAGG C^CTAGG
BalI 356 TGGCCA TGG^CCA
BbsI 490 GTCTTC GAAGAC(2/6)
BcgI 962 GCACAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 538 CTAG C^TAG
BlnI 537 CCTAGG C^CTAGG
BpuAI 490 GTCTTC GAAGAC(2/6)
BsaWI 894 TCCGGA [AT]^CCGG[AT]
BseAI 894 TCCGGA T^CCGGA
BsiHKAI 960 GAGCAC G[AT]GC[AT]^C
BsmBI 318 CGTCTC CGTCTC(1/5)
BspEI 894 TCCGGA T^CCGGA
CpoI 891 CGGTCCG CG^G[AT]CCG
EarI 871 GAAGAG CTCTTC(1/4)
EcoT14I 537 CCTAGG C^C[AT][AT]GG
FspI 119 TGCGCA TGC^GCA
HphI 363 GGTGA GGTGA(8/7)
KpnI 469 GGTACC GGTAC^C
Ksp632I 871 GAAGAG CTCTTC(1/4)
MaeI 538 CTAG C^TAG
MaeIII 364 GTGAC ^GT[ACGT]AC
MluNI 356 TGGCCA TGG^CCA
MroI 894 TCCGGA T^CCGGA
MscI 356 TGGCCA TGG^CCA
NlaIII 948 CATG CATG^
PshAI 366 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 891 CGGTCCG CG^G[AT]CCG
SmlI 715 CTCAAG C^T[CT][GA]AG
StyI 537 CCTAGG C^C[AT][AT]GG
TaqI 706 TCGA T^CGA
Tsp45I 364 GTGAC ^GT[GC]AC
TthHB8I 706 TCGA T^CGA
XcmI 235 CCACCCGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AfaI 470 GTAC GT^AC
560 GTAC
Aor51HI 308 AGCGCT AGC^GCT
653 AGCGCT
BanII 619 GAGCCC G[GA]GC[CT]^C
907 GAGCCC
BbeI 192 GGCGCC GGCGC^C
696 GGCGCC
BglII 50 AGATCT A^GATCT
917 AGATCT
BsiEI 227 CGGCCG CG[GA][CT]^CG
566 CGGCCG
BsrI 367 ACTGG ACTGG(1/-1)
824 CCAGT
BssHII 514 GCGCGC G^CGCGC
596 GCGCGC
BssSI 272 CTCGTG CACGAG(-5/-1)
506 CTCGTG
BstYI 50 AGATCT [GA]^GATC[CT]
917 AGATCT
DpnII 51 GATC ^GATC
918 GATC
DraII 184 GGGACCC [GA]G^G[ACGT]CC[CT]
548 AGGACCC
EagI 227 CGGCCG C^GGCCG
566 CGGCCG
EclXI 227 CGGCCG C^GGCCG
566 CGGCCG
Eco47III 308 AGCGCT AGC^GCT
653 AGCGCT
Eco52I 227 CGGCCG C^GGCCG
566 CGGCCG
EcoO109I 184 GGGACCC [GA]G^G[ACGT]CC[CT]
548 AGGACCC
HinfI 371 GAGTC G^A[ACGT]TC
836 GACTC
KasI 192 GGCGCC G^GCGCC
696 GGCGCC
KspI 331 CCGCGG CCGC^GG
635 CCGCGG
MaeII 380 ACGT A^CGT
794 ACGT
MboI 51 GATC ^GATC
918 GATC
MflI 50 AGATCT [GA]^GATC[CT]
917 AGATCT
NaeI 222 GCCGGC GCC^GGC
241 GCCGGC
NarI 192 GGCGCC GG^CGCC
696 GGCGCC
NdeII 51 GATC ^GATC
918 GATC
NgoMIV 222 GCCGGC G^CCGGC
241 GCCGGC
PleI 836 GACTC GAGTC(4/5)
371 GAGTC
PpuMI 184 GGGACCC [GA]G^G[AT]CC[CT]
548 AGGACCC
RsaI 470 GTAC GT^AC
560 GTAC
SacII 331 CCGCGG CCGC^GG
635 CCGCGG
Sau3AI 51 GATC ^GATC
918 GATC
SfoI 192 GGCGCC GGC^GCC
696 GGCGCC
XhoII 50 AGATCT [GA]^GATC[CT]
917 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BbvI BcnI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DsaI EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIV
Sau96I ScrFI SfaNI SmaI TseI
XmaCI XmaI