(Circular) (Six-base) MAPSORT of: pFN21ASDB0021.seq Check: 2384 from: 1 to: 5710 pFN21ASDB0021 5710 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2160 3053 1937 Size: 223 893 4594 Fragments arranged by size: 4594 893 223 Acc65I G'GTAC_C Cuts at: 2492 3031 2492 Size: 539 5171 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3773 5157 5425 1632 Size: 2141 1384 268 1917 Fragments arranged by size: 2141 1917 1384 268 AfeI AGC'GCT Cuts at: 2333 2678 2333 Size: 345 5365 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2126 1184 Size: 942 4768 AlwNI CAG_nnn'CTG Cuts at: 3453 5025 3453 Size: 1572 4138 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 3019 3245 3397 5506 1493 Size: 1526 226 152 2109 1697 Fragments arranged by size: 2109 1697 1526 226 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2237 2519 2854 2876 2933 3035 1870 Size: 73 294 282 335 22 57 102 4545 Fragments arranged by size: 4545 335 294 282 102 73 57 22 AvrII C'CTAG_G Cuts at: 2560 2560 Size: 5710 BamHI G'GATC_C Cuts at: 3040 3407 3040 Size: 367 5343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2215 2371 2492 2719 3031 Size: 325 217 627 428 156 121 227 312 Cuts at: 3031 4486 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 428 325 312 227 217 156 121 BanII G_rGCy'C Cuts at: 1391 2646 2934 3029 1391 Size: 1255 288 95 4072 Fragments arranged by size: 4072 1255 288 95 BbeI G_GCGC'C Cuts at: 2219 2723 2219 Size: 504 5206 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2506 928 Size: 971 607 4132 Fragments arranged by size: 4132 971 607 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2974 3008 3901 3935 1903 Size: 34 1037 34 893 34 3678 Fragments arranged by size: 3678 1037 893 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2550 2562 2709 Size: 107 122 71 631 605 878 12 147 Cuts at: 2709 2880 2996 4321 136 Size: 171 116 1325 1525 Fragments arranged by size: 1525 1325 878 631 605 171 147 122 116 107 71 12 BglII A'GATC_T Cuts at: 2073 2940 5705 2073 Size: 867 2765 2078 Fragments arranged by size: 2765 2078 867 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 2180 2669 3771 4927 1790 Size: 390 489 1102 1156 2573 Fragments arranged by size: 2573 1156 1102 489 390 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2353 2353 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2412 2464 3001 4370 1239 Size: 818 355 52 537 1369 2579 Fragments arranged by size: 2579 1369 818 537 355 52 Bpu10I CC'TnA_GC Cuts at: 2165 2165 Size: 5710 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2723 3835 4700 4998 2723 Size: 1112 865 298 3435 Fragments arranged by size: 3435 1112 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2134 2306 2801 4373 882 Size: 617 635 172 495 1572 2219 Fragments arranged by size: 2219 1572 635 617 495 172 BsaAI yAC'GTr Cuts at: 493 1204 3007 3501 493 Size: 711 1803 494 2702 Fragments arranged by size: 2702 1803 711 494 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2216 2511 2720 3517 Size: 53 83 186 936 683 295 209 797 Cuts at: 3517 3899 4534 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 797 683 635 382 295 209 186 83 53 BsaWI w'CCGG_w Cuts at: 2917 4143 4541 4815 4962 5373 2917 Size: 1226 398 274 147 411 3254 Fragments arranged by size: 3254 1226 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2326 2793 2813 2912 2921 1186 Size: 1140 467 20 99 9 3975 Fragments arranged by size: 3975 1140 467 99 20 9 BseYI C'CCAG_C Cuts at: 2204 2268 2505 2704 4913 5409 2204 Size: 64 237 199 2209 496 2505 Fragments arranged by size: 2505 2209 496 237 199 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5137 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2253 2592 3160 3921 4949 1540 Size: 275 186 252 339 568 761 1028 2301 Fragments arranged by size: 2301 1028 761 568 339 275 252 186 BsiHKAI G_wGCw'C Cuts at: 2987 3771 3856 4927 2987 Size: 784 85 1071 3770 Fragments arranged by size: 3770 1071 784 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 BsmBI CGTCTCn'nnnn_ Cuts at: 2347 2347 Size: 5710 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2180 2646 2669 2934 2987 3029 3771 Size: 399 390 466 23 265 53 42 742 Cuts at: 3771 3856 4927 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 466 399 390 265 85 53 42 23 BspEI T'CCGG_A Cuts at: 2917 5373 2917 Size: 2456 3254 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3065 844 Size: 2221 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2294 2737 2913 3597 1394 Size: 429 471 443 176 684 3507 Fragments arranged by size: 3507 684 471 443 429 176 BsrDI GCAATG_nn' Cuts at: 65 1248 4205 4379 65 Size: 1183 2957 174 1396 Fragments arranged by size: 2957 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2195 2245 2264 2374 2587 2956 4354 Size: 110 385 50 19 110 213 369 1398 Cuts at: 4354 4541 1700 Size: 187 2869 Fragments arranged by size: 2869 1398 385 369 213 187 110 110 50 19 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 2537 2619 1925 Size: 612 82 5016 Fragments arranged by size: 5016 612 82 BssSI C'ACGA_G Cuts at: 2295 2529 3770 4782 2295 Size: 234 1241 1012 3223 Fragments arranged by size: 3223 1241 1012 234 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 2007 4514 2007 Size: 2507 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2073 2940 3040 3407 3792 3809 5261 5347 Size: 101 867 100 367 385 17 1452 86 Cuts at: 5347 5359 5705 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 867 385 367 346 101 100 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2354 2382 2658 513 Size: 552 957 332 28 276 3565 Fragments arranged by size: 3565 957 552 332 276 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2030 487 Size: 683 56 804 4167 Fragments arranged by size: 4167 804 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2250 2306 Size: 54 1425 50 275 18 363 57 56 Cuts at: 2306 2379 2475 2589 3157 4046 5675 8 Size: 73 96 114 568 889 1629 43 Fragments arranged by size: 1629 1425 889 568 363 275 114 96 73 57 56 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2250 2589 3157 1537 Size: 275 438 339 568 4090 Fragments arranged by size: 4090 568 438 339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2889 3640 1353 Size: 1536 751 3423 Fragments arranged by size: 3423 1536 751 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2430 2438 4297 4671 4817 387 Size: 1064 138 841 8 1859 374 146 1280 Fragments arranged by size: 1859 1280 1064 841 374 146 138 8 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2412 2464 3001 3773 4370 5157 Size: 393 425 355 52 537 772 597 787 Cuts at: 5157 5425 1239 Size: 268 1524 Fragments arranged by size: 1524 787 772 597 537 425 393 355 268 52 EcoO109I rG'GnC_Cy Cuts at: 2208 2572 2208 Size: 364 5346 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 2144 4216 2144 Size: 2072 3638 HaeII r_GCGC'y Cuts at: 1678 2151 2219 2335 2680 2723 4857 1678 Size: 473 68 116 345 43 2134 2531 Fragments arranged by size: 2531 2134 473 345 116 68 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4351 4383 4425 4457 1373 Size: 32 135 32 2779 32 42 32 2626 Fragments arranged by size: 2779 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KasI G'GCGC_C Cuts at: 2215 2719 2215 Size: 504 5206 KpnI G_GTAC'C Cuts at: 2496 3035 2496 Size: 539 5171 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4824 5008 1305 Size: 3519 184 2007 Fragments arranged by size: 3519 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 10 Size: 54 1425 343 549 3339 Fragments arranged by size: 3339 1425 549 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3668 4027 4186 518 Size: 648 2502 359 159 2042 Fragments arranged by size: 2502 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2204 2356 2633 2660 2708 3126 3803 4951 Size: 598 152 277 27 48 418 677 1148 Cuts at: 4951 5196 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 598 418 277 245 152 48 27 NaeI GCC'GGC Cuts at: 2247 2266 2247 Size: 19 5691 NarI GG'CG_CC Cuts at: 2216 2720 2216 Size: 504 5206 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4201 Fragments arranged by size: 4201 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NgoMIV G'CCGG_C Cuts at: 2245 2264 2245 Size: 19 5691 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 3068 4613 1132 Size: 97 1839 1545 2229 Fragments arranged by size: 2229 1839 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5710 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2208 2572 2208 Size: 364 5346 PshAI GACnn'nnGTC Cuts at: 1613 2393 1613 Size: 780 4930 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PstI C_TGCA'G Cuts at: 830 3062 830 Size: 2232 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 RsrII CG'GwC_CG Cuts at: 2915 2915 Size: 5710 SacII CC_GC'GG Cuts at: 2357 2661 2357 Size: 304 5406 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SanDI GG'GwC_CC Cuts at: 2208 2208 Size: 5710 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 3058 4193 4874 5065 826 Size: 219 2013 1135 681 191 1471 Fragments arranged by size: 2013 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2217 2721 2217 Size: 504 5206 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 2239 2878 2935 3037 1872 Size: 367 639 57 102 4545 Fragments arranged by size: 4545 639 367 102 57 SmlI C'TyrA_G Cuts at: 820 1017 1943 2738 3814 4715 4977 820 Size: 197 926 795 1076 901 262 1553 Fragments arranged by size: 1553 1076 926 901 795 262 197 SnaBI TAC'GTA Cuts at: 493 3007 3501 493 Size: 2514 494 2702 Fragments arranged by size: 2702 2514 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SrfI GCCC'GGGC Cuts at: 2935 2935 Size: 5710 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2022 2560 3149 513 Size: 552 957 538 589 3074 Fragments arranged by size: 3074 957 589 552 538 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2330 3737 3754 3907 4092 4537 325 Size: 1255 750 1407 17 153 185 445 1498 Fragments arranged by size: 1498 1407 1255 750 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3956 5668 96 Size: 274 80 33 51 1443 1979 1712 138 Fragments arranged by size: 1979 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2265 2265 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 2237 2876 2933 3035 1870 Size: 367 639 57 102 4545 Fragments arranged by size: 4545 639 367 102 57 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII SacI SapI SexAI SfiI SwaI