Restriction map for
pFN21ASDB0021
(Linear) (Six-base) MAPSORT of: KIBB0021.seq Check: 8647 from: 1 to: 981
KIBB0021.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 137 981
Size: 137 844
Acc65I G'GTAC_C
Cuts at: 0 469 981
Size: 469 512
AfeI AGC'GCT
Cuts at: 0 310 655 981
Size: 310 345 326
Fragments arranged by size:
345 326 310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 103 981
Size: 103 878
AvaI C'yCGr_G
Cuts at: 0 214 496 831 853 910 981
Size: 214 282 335 22 57 71
Fragments arranged by size:
335 282 214 71 57 22
AvrII C'CTAG_G
Cuts at: 0 537 981
Size: 537 444
BanI G'GyrC_C
Cuts at: 0 192 348 469 696 981
Size: 192 156 121 227 285
Fragments arranged by size:
285 227 192 156 121
BanII G_rGCy'C
Cuts at: 0 623 911 981
Size: 623 288 70
Fragments arranged by size:
623 288 70
BbeI G_GCGC'C
Cuts at: 0 196 700 981
Size: 196 504 281
Fragments arranged by size:
504 281 196
BbsI GAAGACnn'nnnn_
Cuts at: 0 483 981
Size: 483 498
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 951 981
Size: 951 30
BglI GCCn_nnn'nGGC
Cuts at: 0 527 539 686 857 973 981
Size: 527 12 147 171 116 8
Fragments arranged by size:
527 171 147 116 12 8
BglII A'GATC_T
Cuts at: 0 50 917 981
Size: 50 867 64
Fragments arranged by size:
867 64 50
Bme1580I G_kGCm'C
Cuts at: 0 157 646 981
Size: 157 489 335
Fragments arranged by size:
489 335 157
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 330 981
Size: 330 651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 389 441 978 981
Size: 34 355 52 537 3
Fragments arranged by size:
537 355 52 34 3
Bpu10I CC'TnA_GC
Cuts at: 0 142 981
Size: 142 839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 981
Size: 700 281
BsaI GGTCTCn'nnnn_
Cuts at: 0 111 283 778 981
Size: 111 172 495 203
Fragments arranged by size:
495 203 172 111
BsaHI Gr'CG_yC
Cuts at: 0 193 488 697 981
Size: 193 295 209 284
Fragments arranged by size:
295 284 209 193
BsaWI w'CCGG_w
Cuts at: 0 894 981
Size: 894 87
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 303 770 790 889 898 981
Size: 303 467 20 99 9 83
Fragments arranged by size:
467 303 99 83 20 9
BseYI C'CCAG_C
Cuts at: 0 181 245 482 681 981
Size: 181 64 237 199 300
Fragments arranged by size:
300 237 199 181 64
BsiEI CG_ry'CG
Cuts at: 0 230 569 981
Size: 230 339 412
Fragments arranged by size:
412 339 230
BsiHKAI G_wGCw'C
Cuts at: 0 964 981
Size: 964 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 324 981
Size: 324 657
Bsp1286I G_dGCh'C
Cuts at: 0 157 623 646 911 964 981
Size: 157 466 23 265 53 17
Fragments arranged by size:
466 265 157 53 23 17
BspEI T'CCGG_A
Cuts at: 0 894 981
Size: 894 87
BsrBI CCG'CTC
Cuts at: 0 271 714 890 981
Size: 271 443 176 91
Fragments arranged by size:
443 271 176 91
BsrFI r'CCGG_y
Cuts at: 0 172 222 241 351 564 933 981
Size: 172 50 19 110 213 369 48
Fragments arranged by size:
369 213 172 110 50 48 19
BssHII G'CGCG_C
Cuts at: 0 514 596 981
Size: 514 82 385
Fragments arranged by size:
514 385 82
BssSI C'ACGA_G
Cuts at: 0 272 506 981
Size: 272 234 475
Fragments arranged by size:
475 272 234
BstYI r'GATC_y
Cuts at: 0 50 917 981
Size: 50 867 64
Fragments arranged by size:
867 64 50
BtgI C'CryG_G
Cuts at: 0 331 359 635 981
Size: 331 28 276 346
Fragments arranged by size:
346 331 276 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 7 981
Size: 7 974
EaeI y'GGCC_r
Cuts at: 0 170 227 283 356 452 566 981
Size: 170 57 56 73 96 114 415
Fragments arranged by size:
415 170 114 96 73 57 56
EagI C'GGCC_G
Cuts at: 0 227 566 981
Size: 227 339 415
Fragments arranged by size:
415 339 227
EarI CTCTTCn'nnn_
Cuts at: 0 866 981
Size: 866 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 407 415 981
Size: 407 8 566
Fragments arranged by size:
566 407 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 389 441 978 981
Size: 34 355 52 537 3
Fragments arranged by size:
537 355 52 34 3
EcoO109I rG'GnC_Cy
Cuts at: 0 185 549 981
Size: 185 364 432
Fragments arranged by size:
432 364 185
FspI TGC'GCA
Cuts at: 0 121 981
Size: 121 860
HaeII r_GCGC'y
Cuts at: 0 128 196 312 657 700 981
Size: 128 68 116 345 43 281
Fragments arranged by size:
345 281 128 116 68 43
KasI G'GCGC_C
Cuts at: 0 192 696 981
Size: 192 504 285
Fragments arranged by size:
504 285 192
KpnI G_GTAC'C
Cuts at: 0 473 981
Size: 473 508
MscI TGG'CCA
Cuts at: 0 358 981
Size: 358 623
MspA1I CmG'CkG
Cuts at: 0 181 333 610 637 685 981
Size: 181 152 277 27 48 296
Fragments arranged by size:
296 277 181 152 48 27
NaeI GCC'GGC
Cuts at: 0 224 243 981
Size: 224 19 738
Fragments arranged by size:
738 224 19
NarI GG'CG_CC
Cuts at: 0 193 697 981
Size: 193 504 284
Fragments arranged by size:
504 284 193
NgoMIV G'CCGG_C
Cuts at: 0 222 241 981
Size: 222 19 740
Fragments arranged by size:
740 222 19
PpuMI rG'GwC_Cy
Cuts at: 0 185 549 981
Size: 185 364 432
Fragments arranged by size:
432 364 185
PshAI GACnn'nnGTC
Cuts at: 0 370 981
Size: 370 611
RsrII CG'GwC_CG
Cuts at: 0 892 981
Size: 892 89
SacII CC_GC'GG
Cuts at: 0 334 638 981
Size: 334 304 343
Fragments arranged by size:
343 334 304
SanDI GG'GwC_CC
Cuts at: 0 185 981
Size: 185 796
SfoI GGC'GCC
Cuts at: 0 194 698 981
Size: 194 504 283
Fragments arranged by size:
504 283 194
SmaI CCC'GGG
Cuts at: 0 216 855 912 981
Size: 216 639 57 69
Fragments arranged by size:
639 216 69 57
SmlI C'TyrA_G
Cuts at: 0 715 981
Size: 715 266
SrfI GCCC'GGGC
Cuts at: 0 912 981
Size: 912 69
StyI C'CwwG_G
Cuts at: 0 537 981
Size: 537 444
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 307 981
Size: 307 674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 242 981
Size: 242 739
XmaI C'CCGG_G
Cuts at: 0 214 853 910 981
Size: 214 639 57 71
Fragments arranged by size:
639 214 71 57
Enzymes that do cut:
AccI Acc65I AfeI AlfI AvaI AvrII BanI BanII
BbeI BbsI BcgI BglI BglII Bme1580I BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrFI BssHII BssSI
BstYI BtgI BtgZI EaeI EagI EarI EciI Eco57MI
EcoO109I FspI HaeII KasI KpnI MscI MspA1I NaeI
NarI NgoMIV PpuMI PshAI RsrII SacII SanDI SfoI
SmaI SmlI SrfI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI BaeI BamHI BbvCI BciVI BclI BfrBI BlpI
BmgBI BmrI BmtI BsaAI BsaBI BsaXI BsgI BsiWI
BsmI BspHI BspMI BsrDI BsrGI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
Hin4I HincII HindIII HpaI MfeI MluI MmeI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmnI ZraI