(Circular) (Six-base) MAPSORT of: pFN21ASDB0010.seq Check: 3498 from: 1 to: 6586 pFN21ASDB0010 6586 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4396 278 Size: 53 83 186 936 2860 2468 Fragments arranged by size: 2860 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3360 3929 1937 Size: 1423 569 4594 Fragments arranged by size: 4594 1423 569 Acc65I G'GTAC_C Cuts at: 2153 3907 2153 Size: 1754 4832 AclI AA'CG_TT Cuts at: 1563 4713 5086 1563 Size: 3150 373 3063 Fragments arranged by size: 3150 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2574 3292 4649 6033 6301 1632 Size: 942 718 1357 1384 268 1917 Fragments arranged by size: 1917 1384 1357 942 718 268 AfeI AGC'GCT Cuts at: 3134 3467 3134 Size: 333 6253 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6389 AflIII A'CryG_T Cuts at: 1203 5485 6462 1203 Size: 4282 977 1327 Fragments arranged by size: 4282 1327 977 AgeI A'CCGG_T Cuts at: 5417 5417 Size: 6586 AhdI GACnn_n'nnGTC Cuts at: 5315 5315 Size: 6586 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6586 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6586 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2196 2228 2196 Size: 32 6554 AlwNI CAG_nnn'CTG Cuts at: 2168 2971 3040 3491 4329 5901 2168 Size: 803 69 451 838 1572 2853 Fragments arranged by size: 2853 1572 838 803 451 69 ApaI G_GGCC'C Cuts at: 2124 2361 2531 2124 Size: 237 170 6179 Fragments arranged by size: 6179 237 170 ApaLI G'TGCA_C Cuts at: 4643 5799 4643 Size: 1156 5430 ApoI r'AATT_y Cuts at: 1493 3651 3895 4121 4273 6382 1493 Size: 2158 244 226 152 2109 1697 Fragments arranged by size: 2158 2109 1697 244 226 152 AseI AT'TA_AT Cuts at: 160 1032 5140 160 Size: 872 4108 1606 Fragments arranged by size: 4108 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 AvaI C'yCGr_G Cuts at: 1870 1943 2726 3261 3911 1870 Size: 73 783 535 650 4545 Fragments arranged by size: 4545 783 650 535 73 BamHI G'GATC_C Cuts at: 3459 3916 4283 3459 Size: 457 367 5762 Fragments arranged by size: 5762 457 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 2787 2976 3907 5362 Size: 325 217 627 366 634 189 931 1455 Cuts at: 5362 618 Size: 1842 Fragments arranged by size: 1842 1455 931 634 627 366 325 217 189 BanII G_rGCy'C Cuts at: 1391 2124 2227 2361 2531 2961 3644 3751 3905 Size: 733 103 134 170 430 683 107 154 Cuts at: 3905 1391 Size: 4072 Fragments arranged by size: 4072 733 683 430 170 154 134 107 103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2726 3274 928 Size: 971 827 548 4240 Fragments arranged by size: 4240 971 827 548 BbvCI CC'TCA_GC Cuts at: 2324 2324 Size: 6586 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4777 4811 1903 Size: 34 2840 34 3678 Fragments arranged by size: 3678 2840 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2058 3076 4478 5688 2058 Size: 1018 1402 1210 2956 Fragments arranged by size: 2956 1402 1210 1018 BclI T'GATC_A Cuts at: 1548 1548 Size: 6586 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2708 3625 5197 Size: 107 122 71 631 605 1036 917 1572 Cuts at: 5197 136 Size: 1525 Fragments arranged by size: 1572 1525 1036 917 631 605 122 107 71 BglII A'GATC_T Cuts at: 3528 6581 3528 Size: 3053 3533 BlpI GC'TnA_GC Cuts at: 2847 4003 2847 Size: 1156 5430 Bme1580I G_kGCm'C Cuts at: 1790 2124 2361 2531 2979 3129 4647 5803 1790 Size: 334 237 170 448 150 1518 1156 2573 Fragments arranged by size: 2573 1518 1156 448 334 237 170 150 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6586 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2078 4317 5275 Size: 416 53 83 399 24 661 2239 958 Cuts at: 5275 442 Size: 1753 Fragments arranged by size: 2239 1753 958 661 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6586 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2154 2154 Size: 6586 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2553 3264 3416 3424 3651 3795 5246 1239 Size: 1314 711 152 8 227 144 1451 2579 Fragments arranged by size: 2579 1451 1314 711 227 152 144 8 Bpu10I CC'TnA_GC Cuts at: 2324 2715 3192 2324 Size: 391 477 5718 Fragments arranged by size: 5718 477 391 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3029 3041 4711 5576 5874 3029 Size: 12 1670 865 298 3741 Fragments arranged by size: 3741 1670 865 298 12 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5249 882 Size: 617 3750 2219 Fragments arranged by size: 3750 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2665 3883 4377 493 Size: 711 1461 1218 494 2702 Fragments arranged by size: 2702 1461 1218 711 494 BsaBI GATnn'nnATC Cuts at: 6580 6580 Size: 6586 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4393 4775 5410 275 Size: 53 83 186 936 2860 382 635 1451 Fragments arranged by size: 2860 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2061 2288 5019 5417 5691 5838 6249 2061 Size: 227 2731 398 274 147 411 2398 Fragments arranged by size: 2731 2398 411 398 274 227 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2552 2582 3101 3131 3315 Size: 30 165 30 1149 30 519 30 184 Cuts at: 3315 3345 1178 Size: 30 4419 Fragments arranged by size: 4419 1149 519 184 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2389 2501 2573 2603 2969 2990 3339 3342 Size: 1203 112 72 30 366 21 349 3 Cuts at: 3342 3798 1186 Size: 456 3974 Fragments arranged by size: 3974 1203 456 366 349 112 72 30 21 3 BseYI C'CCAG_C Cuts at: 2312 2971 3074 3154 5789 6285 2312 Size: 659 103 80 2635 496 2613 Fragments arranged by size: 2635 2613 659 496 103 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3035 1163 Size: 573 1299 4714 Fragments arranged by size: 4714 1299 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4036 4797 5825 1540 Size: 275 186 2035 761 1028 2301 Fragments arranged by size: 2301 2035 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2961 4647 4732 5803 2961 Size: 1686 85 1071 3744 Fragments arranged by size: 3744 1686 1071 85 BsiWI C'GTAC_G Cuts at: 2174 2174 Size: 6586 BsmI GAATG_Cn' Cuts at: 4097 4190 4097 Size: 93 6493 BsmBI CGTCTCn'nnnn_ Cuts at: 3580 3580 Size: 6586 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2227 2361 2531 2961 2979 3129 Size: 399 334 103 134 170 430 18 150 Cuts at: 3129 3644 3751 3905 4647 4732 5803 1391 Size: 515 107 154 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 515 430 399 334 170 154 150 134 107 103 85 18 BspEI T'CCGG_A Cuts at: 6249 6249 Size: 6586 BspHI T'CATG_A Cuts at: 3024 4475 6205 3024 Size: 1451 1730 3405 Fragments arranged by size: 3405 1730 1451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3941 844 Size: 3097 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4473 1394 Size: 429 2650 3507 Fragments arranged by size: 3507 2650 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2247 3560 5081 5255 65 Size: 1183 999 1313 1521 174 1396 Fragments arranged by size: 1521 1396 1313 1183 999 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2871 5230 5417 1700 Size: 110 1061 2359 187 2869 Fragments arranged by size: 2869 2359 1061 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6586 BssHII G'CGCG_C Cuts at: 1925 2092 1925 Size: 167 6419 BssSI C'ACGA_G Cuts at: 2864 4646 5658 2864 Size: 1782 1012 3792 Fragments arranged by size: 3792 1782 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2983 3491 794 Size: 2189 508 3889 Fragments arranged by size: 3889 2189 508 BstBI TT'CG_AA Cuts at: 2007 5390 2007 Size: 3383 3203 BstEII G'GTnAC_C Cuts at: 1184 3226 3413 1184 Size: 2042 187 4357 Fragments arranged by size: 4357 2042 187 BstXI CCAn_nnnn'nTGG Cuts at: 2521 3610 2521 Size: 1089 5497 BstYI r'GATC_y Cuts at: 1972 2830 3459 3528 3916 4283 4668 4685 6137 Size: 858 629 69 388 367 385 17 1452 Cuts at: 6137 6223 6235 6581 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 858 629 388 385 367 346 86 69 17 12 BstZ17I GTA'TAC Cuts at: 3361 3361 Size: 6586 Bsu36I CC'TnA_GG Cuts at: 2372 3237 2372 Size: 865 5721 BtgI C'CryG_G Cuts at: 513 1065 2706 513 Size: 552 1641 4393 Fragments arranged by size: 4393 1641 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5847 Fragments arranged by size: 5847 683 56 BtsI GCAGTG_nn' Cuts at: 2263 4102 4896 4916 2263 Size: 1839 794 20 3933 Fragments arranged by size: 3933 1839 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6586 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6551 DraI TTT'AAA Cuts at: 3890 3890 Size: 6586 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5593 809 Size: 507 4277 1802 Fragments arranged by size: 4277 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2050 2700 3617 Size: 54 1425 50 275 18 220 650 917 Cuts at: 3617 3741 4033 4922 6551 8 Size: 124 292 889 1629 43 Fragments arranged by size: 1629 1425 917 889 650 292 275 220 124 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4033 1537 Size: 275 2221 4090 Fragments arranged by size: 4090 2221 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2550 4516 1353 Size: 1197 1966 3423 Fragments arranged by size: 3423 1966 1197 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5173 5547 5693 387 Size: 1064 138 3584 374 146 1280 Fragments arranged by size: 3584 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2959 2959 Size: 6586 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2553 2574 3264 3292 3416 3424 3651 Size: 393 921 21 690 28 124 8 227 Cuts at: 3651 3795 4649 5246 6033 6301 1239 Size: 144 854 597 787 268 1524 Fragments arranged by size: 1524 921 854 787 690 597 393 268 227 144 124 28 21 8 EcoO109I rG'GnC_Cy Cuts at: 2120 2121 2212 2348 2357 2527 2528 2722 3626 Size: 1 91 136 9 170 1 194 904 Cuts at: 3626 3772 2120 Size: 146 4934 Fragments arranged by size: 4934 904 194 170 146 136 91 9 1 1 EcoRI G'AATT_C Cuts at: 3651 3895 4273 3651 Size: 244 378 5964 Fragments arranged by size: 5964 378 244 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3869 3869 Size: 6586 FspI TGC'GCA Cuts at: 5092 5092 Size: 6586 HaeII r_GCGC'y Cuts at: 1678 2872 3136 3469 5733 1678 Size: 1194 264 333 2264 2531 Fragments arranged by size: 2531 2264 1194 333 264 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2150 2182 2552 2584 5227 Size: 32 135 32 578 32 370 32 2643 Cuts at: 5227 5259 5301 5333 1373 Size: 32 42 32 2626 Fragments arranged by size: 2643 2626 578 370 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3930 4176 669 Size: 988 281 1992 246 3079 Fragments arranged by size: 3079 1992 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6586 HpaI GTT'AAC Cuts at: 4176 4176 Size: 6586 KpnI G_GTAC'C Cuts at: 2157 3911 2157 Size: 1754 4832 MfeI C'AATT_G Cuts at: 4185 4185 Size: 6586 MluI A'CGCG_T Cuts at: 6462 6462 Size: 6586 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2364 2442 2502 3845 5700 5884 1305 Size: 1059 78 60 1343 1855 184 2007 Fragments arranged by size: 2007 1855 1343 1059 184 78 60 MscI TGG'CCA Cuts at: 10 64 1489 1832 2702 3743 10 Size: 54 1425 343 870 1041 2853 Fragments arranged by size: 2853 1425 1041 870 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2883 3377 4544 4903 5062 518 Size: 648 1717 494 1167 359 159 2042 Fragments arranged by size: 2042 1717 1167 648 494 359 159 MspA1I CmG'CkG Cuts at: 1606 2971 2989 3019 3037 4002 4679 5827 6072 Size: 1365 18 30 18 965 677 1148 245 Cuts at: 6072 1606 Size: 2120 Fragments arranged by size: 2120 1365 1148 965 677 245 30 18 18 NaeI GCC'GGC Cuts at: 2873 2873 Size: 6586 NcoI C'CATG_G Cuts at: 513 1065 2706 513 Size: 552 1641 4393 Fragments arranged by size: 4393 1641 552 NdeI CA'TA_TG Cuts at: 387 2446 2880 387 Size: 2059 434 4093 Fragments arranged by size: 4093 2059 434 NgoMIV G'CCGG_C Cuts at: 2871 2871 Size: 6586 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6586 NotI GC'GGCC_GC Cuts at: 4033 4033 Size: 6586 NruI TCG'CGA Cuts at: 2202 2202 Size: 6586 NspI r_CATG'y Cuts at: 1132 1229 2089 2100 2924 3521 3944 5489 1132 Size: 97 860 11 824 597 423 1545 2229 Fragments arranged by size: 2229 1545 860 824 597 423 97 11 PasI CC'CwG_GG Cuts at: 1348 2108 2354 3319 3673 1348 Size: 760 246 965 354 4261 Fragments arranged by size: 4261 965 760 354 246 PciI A'CATG_T Cuts at: 5485 5485 Size: 6586 PflMI CCAn_nnn'nTGG Cuts at: 1108 3455 1108 Size: 2347 4239 PmeI GTTT'AAAC Cuts at: 3890 3890 Size: 6586 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4655 4687 6168 6200 4655 Size: 32 1481 32 5041 Fragments arranged by size: 5041 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2212 2722 2212 Size: 510 6076 PshAI GACnn'nnGTC Cuts at: 1613 2430 1613 Size: 817 5769 PsiI TTA'TAA Cuts at: 4156 4156 Size: 6586 PspOMI G'GGCC_C Cuts at: 2120 2357 2527 2120 Size: 237 170 6179 Fragments arranged by size: 6179 237 170 PstI C_TGCA'G Cuts at: 830 2232 2988 3091 3480 3487 3938 830 Size: 1402 756 103 389 7 451 3478 Fragments arranged by size: 3478 1402 756 451 389 103 7 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6586 PvuII CAG'CTG Cuts at: 2971 2989 3037 2971 Size: 18 48 6520 Fragments arranged by size: 6520 48 18 RsrII CG'GwC_CG Cuts at: 2035 2035 Size: 6586 SacI G_AGCT'C Cuts at: 2961 2961 Size: 6586 SalI G'TCGA_C Cuts at: 1936 3928 1936 Size: 1992 4594 SanDI GG'GwC_CC Cuts at: 2722 2722 Size: 6586 SbfI CC_TGCA'GG Cuts at: 3480 3938 3480 Size: 458 6128 ScaI AGT'ACT Cuts at: 4834 6546 4834 Size: 1712 4874 SfcI C'TryA_G Cuts at: 826 1045 2228 2984 3087 3476 3483 3934 5069 Size: 219 1183 756 103 389 7 451 1135 Cuts at: 5069 5750 5941 826 Size: 681 191 1471 Fragments arranged by size: 1471 1183 1135 756 681 451 389 219 191 103 7 SfiI GGCCn_nnn'nGGCC Cuts at: 3625 3625 Size: 6586 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6586 SmaI CCC'GGG Cuts at: 1872 3913 1872 Size: 2041 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3044 3056 4690 5591 5853 820 Size: 197 926 1101 12 1634 901 262 1553 Fragments arranged by size: 1634 1553 1101 926 901 262 197 12 SnaBI TAC'GTA Cuts at: 493 3883 4377 493 Size: 3390 494 2702 Fragments arranged by size: 3390 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6586 SphI G_CATG'C Cuts at: 1132 2089 2100 3944 1132 Size: 957 11 1844 3774 Fragments arranged by size: 3774 1844 957 11 SspI AAT'ATT Cuts at: 5 52 4510 5 Size: 47 4458 2081 Fragments arranged by size: 4458 2081 47 StuI AGG'CCT Cuts at: 1517 3506 1517 Size: 1989 4597 StyI C'CwwG_G Cuts at: 513 1065 2126 2706 3409 3775 4025 513 Size: 552 1061 580 703 366 250 3074 Fragments arranged by size: 3074 1061 703 580 552 366 250 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3586 3706 3742 4613 4630 4783 4968 Size: 1255 2006 120 36 871 17 153 185 Cuts at: 4968 5413 325 Size: 445 1498 Fragments arranged by size: 2006 1498 1255 871 445 185 153 120 36 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2892 4832 6544 Size: 274 80 33 51 1443 915 1940 1712 Cuts at: 6544 96 Size: 138 Fragments arranged by size: 1940 1712 1443 915 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3101 3133 3315 3347 6546 6578 3101 Size: 32 182 32 3199 32 3109 Fragments arranged by size: 3199 3109 182 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2566 1316 Size: 1250 5336 XbaI T'CTAG_A Cuts at: 3922 3922 Size: 6586 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2451 3736 2451 Size: 1285 5301 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6586 XmaI C'CCGG_G Cuts at: 1870 3911 1870 Size: 2041 4545 XmnI GAAnn'nnTTC Cuts at: 4715 4715 Size: 6586 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4394 276 Size: 53 83 186 936 2860 2468 Fragments arranged by size: 2860 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI DraIII EcoNI EcoRV FseI FspAI KasI NarI NsiI PacI PfoI PmlI PspXI PsrI SacII SapI SexAI SfoI SrfI SwaI