Restriction map for
pFN21ASDB0008
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI Aor51HI
ApaI AscI AseI AsnI AspI
AvrII BalI BamHI BbeI BcgI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmI BspDI
BspEI BspLU11I BspMI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraII DrdI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI HaeII HgaI
Hin1I HindIII HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XcmI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 1321 GGTACC G^GTACC
AhdI 444 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1531 GTGCAC G^TGCAC
AocI 457 CCTGAGG CC^T[ACGT]AGG
ApaLI 1531 GTGCAC G^TGCAC
Asp700I 1827 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1321 GGTACC G^GTACC
AspEI 444 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 978 TGCGCA TGC^GCA
BanI 1321 GGTACC G^G[CT][GA]CC
BbrPI 1280 CACGTG CAC^GTG
BbvCI 658 GCTGAGG CCTCAGC(-5/-2)
BglI 9 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1280 CACGTG [CT]AC^GT[GA]
BsmBI 805 CGTCTC CGTCTC(1/5)
Bsp1407I 524 TGTACA T^GTACA
BsrBI 414 CCGCTC CCGCTC(-3/-3)
BsrDI 1336 GCAATG GCAATG(2/0)
BsrGI 524 TGTACA T^GTACA
BssSI 718 CACGAG CACGAG(-5/-1)
BstYI 320 AGATCC [GA]^GATC[CT]
Bsu36I 457 CCTGAGG CC^T[ACGT]AGG
CfoI 979 GCGC GCG^C
DraI 1207 TTTAAA TTT^AAA
DraIII 1277 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 444 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 457 CCTGAGG CC^T[ACGT]AGG
FspI 978 TGCGCA TGC^GCA
HhaI 979 GCGC GCG^C
HinP1I 979 GCGC G^CGC
HincII 78 GTTGAC GT[CT]^[GA]AC
HindII 78 GTTGAC GT[CT]^[GA]AC
KpnI 1321 GGTACC GGTAC^C
MflI 320 AGATCC [GA]^GATC[CT]
NcoI 1646 CCATGG C^CATGG
PaeR7I 1111 CTCGAG C^TCGAG
PmaCI 1280 CACGTG CAC^GTG
PmlI 1280 CACGTG CAC^GTG
SacI 94 GAGCTC GAGCT^C
ScaI 1781 AGTACT AGT^ACT
SmaI 1664 CCCGGG CCC^GGG
SmlI 1111 CTCGAG C^T[CT][GA]AG
SspBI 524 TGTACA T^GTACA
StuI 1886 AGGCCT AGG^CCT
XhoI 1111 CTCGAG C^TCGAG
XhoII 320 AGATCC [GA]^GATC[CT]
XmaCI 1664 CCCGGG C^CCGGG
XmaI 1664 CCCGGG C^CCGGG
XmnI 1827 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 86 AAATTC [GA]^AATT[CT]
1204 GAATTT
AlwI 321 GATCC GGATC(4/5)
1135 GATCC
ApoI 86 AAATTC [GA]^AATT[CT]
1204 GAATTT
AvaI 1111 CTCGAG C^[CT]CG[GA]G
1664 CCCGGG
BanII 94 GAGCTC G[GA]GC[CT]^C
310 GAGCCC
BciVI 427 GTATCC GTATCC(6/5)
1142 GTATCC
BfaI 1357 CTAG C^TAG
1466 CTAG
BsaI 1763 GAGACC GGTCTC(1/5)
31 GGTCTC
BsoBI 1111 CTCGAG C^[CT]CG[GA]G
1664 CCCGGG
BspHI 1344 TCATGA T^CATGA
1626 TCATGA
DsaI 821 CCGTGG C^C[GA][CT]GG
1646 CCATGG
MaeI 1357 CTAG C^TAG
1466 CTAG
MaeIII 404 GTGAC ^GT[ACGT]AC
1016 GTCAC
PleI 839 GACTC GAGTC(4/5)
1341 GACTC
RcaI 1344 TCATGA T^CATGA
1626 TCATGA
SapI 745 GAAGAGC GCTCTTC(1/4)
1000 GAAGAGC
SfaNI 712 GATGC GCATC(5/9)
1860 GATGC
Tsp45I 404 GTGAC ^GT[GC]AC
1016 GTCAC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I Van91I