(Circular) (Six-base) MAPSORT of: pFN21ASDB0008.seq Check: 8779 from: 1 to: 6622 pFN21ASDB0008 6622 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4432 278 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3965 1937 Size: 2028 4594 Acc65I G'GTAC_C Cuts at: 3344 3943 3344 Size: 599 6023 AclI AA'CG_TT Cuts at: 1563 4749 5122 1563 Size: 3186 373 3063 Fragments arranged by size: 3186 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2787 2949 3113 4685 6069 6337 1632 Size: 1155 162 164 1572 1384 268 1917 Fragments arranged by size: 1917 1572 1384 1155 268 164 162 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6425 AflIII A'CryG_T Cuts at: 1203 5521 6498 1203 Size: 4318 977 1327 Fragments arranged by size: 4318 1327 977 AgeI A'CCGG_T Cuts at: 5453 5453 Size: 6622 AhdI GACnn_n'nnGTC Cuts at: 2472 5351 2472 Size: 2879 3743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6622 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6622 AlwNI CAG_nnn'CTG Cuts at: 2389 2788 3178 4365 5937 2389 Size: 399 390 1187 1572 3074 Fragments arranged by size: 3074 1572 1187 399 390 ApaLI G'TGCA_C Cuts at: 3554 4679 5835 3554 Size: 1125 1156 4341 Fragments arranged by size: 4341 1156 1125 ApoI r'AATT_y Cuts at: 1493 2109 3227 3931 4157 4309 6418 1493 Size: 616 1118 704 226 152 2109 1697 Fragments arranged by size: 2109 1697 1118 704 616 226 152 AseI AT'TA_AT Cuts at: 160 1032 5176 160 Size: 872 4144 1606 Fragments arranged by size: 4144 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 AvaI C'yCGr_G Cuts at: 1870 1943 3134 3687 3947 1870 Size: 73 1191 553 260 4545 Fragments arranged by size: 4545 1191 553 260 73 BamHI G'GATC_C Cuts at: 3952 4319 3952 Size: 367 6255 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3344 3943 5398 618 Size: 325 217 627 1557 599 1455 1842 Fragments arranged by size: 1842 1557 1455 627 599 325 217 BanII G_rGCy'C Cuts at: 1391 2121 2337 3941 1391 Size: 730 216 1604 4072 Fragments arranged by size: 4072 1604 730 216 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2471 3101 3381 928 Size: 971 572 630 280 4169 Fragments arranged by size: 4169 971 630 572 280 BbvCI CC'TCA_GC Cuts at: 2682 2682 Size: 6622 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4813 4847 1903 Size: 34 2876 34 3678 Fragments arranged by size: 3678 2876 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2461 3176 4514 5724 2461 Size: 715 1338 1210 3359 Fragments arranged by size: 3359 1338 1210 715 BclI T'GATC_A Cuts at: 1548 1548 Size: 6622 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2038 5233 136 Size: 107 122 71 631 605 366 3195 1525 Fragments arranged by size: 3195 1525 631 605 366 122 107 71 BglII A'GATC_T Cuts at: 6617 6617 Size: 6622 BlpI GC'TnA_GC Cuts at: 4039 4039 Size: 6622 Bme1580I G_kGCm'C Cuts at: 1790 3558 4683 5839 1790 Size: 1768 1125 1156 2573 Fragments arranged by size: 2573 1768 1156 1125 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6622 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2449 4353 5311 Size: 416 53 83 399 24 1032 1904 958 Cuts at: 5311 442 Size: 1753 Fragments arranged by size: 1904 1753 1032 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6622 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3676 3676 Size: 6622 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2351 3310 5282 1239 Size: 896 216 959 1972 2579 Fragments arranged by size: 2579 1972 959 896 216 Bpu10I CC'TnA_GC Cuts at: 2682 2682 Size: 6622 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4747 5612 5910 4747 Size: 865 298 5459 Fragments arranged by size: 5459 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2060 3780 5285 882 Size: 617 561 1720 1505 2219 Fragments arranged by size: 2219 1720 1505 617 561 BsaAI yAC'GTr Cuts at: 493 1204 3305 3919 4413 493 Size: 711 2101 614 494 2702 Fragments arranged by size: 2702 2101 711 614 494 BsaBI GATnn'nnATC Cuts at: 6616 6616 Size: 6622 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4429 4811 5446 275 Size: 53 83 186 936 2896 382 635 1451 Fragments arranged by size: 2896 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5055 5453 5727 5874 6285 5055 Size: 398 274 147 411 5392 Fragments arranged by size: 5392 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6397 Fragments arranged by size: 6397 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2603 2834 3263 3878 1186 Size: 1417 231 429 615 3930 Fragments arranged by size: 3930 1417 615 429 231 BseYI C'CCAG_C Cuts at: 2132 2958 5825 6321 2132 Size: 826 2867 496 2433 Fragments arranged by size: 2867 2433 826 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6049 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4072 4833 5861 1540 Size: 275 186 2071 761 1028 2301 Fragments arranged by size: 2301 2071 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 3187 3301 3558 4683 4768 5839 2121 Size: 1066 114 257 1125 85 1071 2904 Fragments arranged by size: 2904 1125 1071 1066 257 114 85 BsmI GAATG_Cn' Cuts at: 4133 4226 4133 Size: 93 6529 BsmBI CGTCTCn'nnnn_ Cuts at: 2834 2834 Size: 6622 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2337 3187 3301 3558 3941 4683 Size: 399 331 216 850 114 257 383 742 Cuts at: 4683 4768 5839 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 850 742 399 383 331 257 216 114 85 BspEI T'CCGG_A Cuts at: 6285 6285 Size: 6622 BspHI T'CATG_A Cuts at: 3367 3649 4511 6241 3367 Size: 282 862 1730 3748 Fragments arranged by size: 3748 1730 862 282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3977 844 Size: 3133 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2439 4509 1394 Size: 429 616 2070 3507 Fragments arranged by size: 3507 2070 616 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3366 5117 5291 65 Size: 1183 2118 1751 174 1396 Fragments arranged by size: 2118 1751 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5266 5453 1700 Size: 110 3456 187 2869 Fragments arranged by size: 3456 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2547 96 Size: 2451 4171 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6622 BssSI C'ACGA_G Cuts at: 2741 4682 5694 2741 Size: 1941 1012 3669 Fragments arranged by size: 3669 1941 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6622 BstBI TT'CG_AA Cuts at: 2007 5426 2007 Size: 3419 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6622 BstYI r'GATC_y Cuts at: 1972 2343 3952 4319 4704 4721 6173 6259 6271 Size: 371 1609 367 385 17 1452 86 12 Cuts at: 6271 6617 1972 Size: 346 1977 Fragments arranged by size: 1977 1609 1452 385 371 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2481 2481 Size: 6622 BtgI C'CryG_G Cuts at: 513 1065 2022 2844 3669 513 Size: 552 957 822 825 3466 Fragments arranged by size: 3466 957 825 822 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2951 487 Size: 683 56 1725 4158 Fragments arranged by size: 4158 1725 683 56 BtsI GCAGTG_nn' Cuts at: 3558 4138 4932 4952 3558 Size: 580 794 20 5228 Fragments arranged by size: 5228 794 580 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6622 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2826 2861 529 Size: 35 2262 35 4290 Fragments arranged by size: 4290 2262 35 35 DraI TTT'AAA Cuts at: 3232 3926 3232 Size: 694 5928 DraIII CAC_nnn'GTG Cuts at: 3305 3305 Size: 6622 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5629 809 Size: 507 4313 1802 Fragments arranged by size: 4313 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4069 4958 6587 Size: 54 1425 50 275 18 2239 889 1629 Cuts at: 6587 8 Size: 43 Fragments arranged by size: 2239 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4069 1537 Size: 275 2257 4090 Fragments arranged by size: 4090 2257 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2412 2763 3018 3512 3778 4552 1353 Size: 1059 351 255 494 266 774 3423 Fragments arranged by size: 3423 1059 774 494 351 266 255 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5209 5583 5729 387 Size: 1064 138 3620 374 146 1280 Fragments arranged by size: 3620 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2119 2119 Size: 6622 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2351 2787 2949 3113 3310 4685 Size: 393 503 216 436 162 164 197 1375 Cuts at: 4685 5282 6069 6337 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1375 787 597 503 436 393 268 216 197 164 162 EcoRI G'AATT_C Cuts at: 3931 4309 3931 Size: 378 6244 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2771 3428 3592 2771 Size: 657 164 5801 Fragments arranged by size: 5801 657 164 FspI TGC'GCA Cuts at: 3003 5128 3003 Size: 2125 4497 HaeII r_GCGC'y Cuts at: 1678 5769 1678 Size: 4091 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2458 2490 2591 2623 2897 Size: 32 135 32 886 32 101 32 274 Cuts at: 2897 2929 3001 3033 5263 5295 5337 5369 1373 Size: 32 72 32 2230 32 42 32 2626 Fragments arranged by size: 2626 2230 886 274 135 101 72 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2103 3966 4212 669 Size: 988 281 165 1863 246 3079 Fragments arranged by size: 3079 1863 988 281 246 165 HindIII A'AGCT_T Cuts at: 748 748 Size: 6622 HpaI GTT'AAC Cuts at: 4212 4212 Size: 6622 KpnI G_GTAC'C Cuts at: 3348 3947 3348 Size: 599 6023 MfeI C'AATT_G Cuts at: 4221 4221 Size: 6622 MluI A'CGCG_T Cuts at: 6498 6498 Size: 6622 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2540 5736 5920 1305 Size: 1235 3196 184 2007 Fragments arranged by size: 3196 2007 1235 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4800 Fragments arranged by size: 4800 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4580 4939 5098 518 Size: 648 3414 359 159 2042 Fragments arranged by size: 3414 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2132 2329 2765 2927 4038 4715 5863 6108 Size: 526 197 436 162 1111 677 1148 245 Cuts at: 6108 1606 Size: 2120 Fragments arranged by size: 2120 1148 1111 677 526 436 245 197 162 NcoI C'CATG_G Cuts at: 513 1065 2022 3669 513 Size: 552 957 1647 3466 Fragments arranged by size: 3466 1647 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6622 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6622 NotI GC'GGCC_GC Cuts at: 4069 4069 Size: 6622 NspI r_CATG'y Cuts at: 1132 1229 3980 5525 1132 Size: 97 2751 1545 2229 Fragments arranged by size: 2751 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2034 1348 Size: 686 5936 PciI A'CATG_T Cuts at: 5521 5521 Size: 6622 PflMI CCAn_nnn'nTGG Cuts at: 1108 2353 2857 3808 1108 Size: 1245 504 951 3922 Fragments arranged by size: 3922 1245 951 504 PfoI T'CCnGG_A Cuts at: 2058 2058 Size: 6622 PmeI GTTT'AAAC Cuts at: 3926 3926 Size: 6622 PmlI CAC'GTG Cuts at: 3305 3305 Size: 6622 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4691 4723 6204 6236 4691 Size: 32 1481 32 5077 Fragments arranged by size: 5077 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6622 PsiI TTA'TAA Cuts at: 4192 4192 Size: 6622 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3788 3820 3788 Size: 32 6590 PstI C_TGCA'G Cuts at: 830 3974 830 Size: 3144 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6622 PvuII CAG'CTG Cuts at: 2132 2329 2765 2927 2132 Size: 197 436 162 5827 Fragments arranged by size: 5827 436 197 162 SacI G_AGCT'C Cuts at: 2121 2121 Size: 6622 SalI G'TCGA_C Cuts at: 1936 3964 1936 Size: 2028 4594 SapI GCTCTTCn'nnn_ Cuts at: 2763 3018 2763 Size: 255 6367 SbfI CC_TGCA'GG Cuts at: 3974 3974 Size: 6622 ScaI AGT'ACT Cuts at: 3806 4870 6582 3806 Size: 1064 1712 3846 Fragments arranged by size: 3846 1712 1064 SfcI C'TryA_G Cuts at: 826 1045 2403 2721 3170 3263 3431 3970 5105 Size: 219 1358 318 449 93 168 539 1135 Cuts at: 5105 5786 5977 826 Size: 681 191 1471 Fragments arranged by size: 1471 1358 1135 681 539 449 318 219 191 168 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6622 SmaI CCC'GGG Cuts at: 1872 3689 3949 1872 Size: 1817 260 4545 Fragments arranged by size: 4545 1817 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 3134 4726 5627 5889 820 Size: 197 926 1191 1592 901 262 1553 Fragments arranged by size: 1592 1553 1191 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3919 4413 493 Size: 3426 494 2702 Fragments arranged by size: 3426 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6622 SphI G_CATG'C Cuts at: 1132 3980 1132 Size: 2848 3774 SspI AAT'ATT Cuts at: 5 52 4546 5 Size: 47 4494 2081 Fragments arranged by size: 4494 2081 47 StuI AGG'CCT Cuts at: 1517 3911 1517 Size: 2394 4228 StyI C'CwwG_G Cuts at: 513 1065 2022 2306 3669 3894 4061 513 Size: 552 957 284 1363 225 167 3074 Fragments arranged by size: 3074 1363 957 552 284 225 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2287 2414 4649 4666 4819 5004 5449 Size: 1255 707 127 2235 17 153 185 445 Cuts at: 5449 325 Size: 1498 Fragments arranged by size: 2235 1498 1255 707 445 185 153 127 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2547 3804 4868 Size: 274 80 33 51 1443 570 1257 1064 Cuts at: 4868 6580 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1257 1064 570 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6582 6614 6582 Size: 32 6590 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6622 XbaI T'CTAG_A Cuts at: 3958 3958 Size: 6622 XhoI C'TCGA_G Cuts at: 1943 3134 1943 Size: 1191 5431 XmaI C'CCGG_G Cuts at: 1870 3687 3947 1870 Size: 1817 260 4545 Fragments arranged by size: 4545 1817 260 XmnI GAAnn'nnTTC Cuts at: 3854 4751 3854 Size: 897 5725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4430 276 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI