Restriction map for
pFN21ASDB0008
(Linear) (Six-base) MAPSORT of: KIBB0008.seq Check: 8441 from: 1 to: 1893
KIBB0008.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
Acc65I G'GTAC_C
Cuts at: 0 1321 1893
Size: 1321 572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 926 1090 1893
Size: 764 162 164 803
Fragments arranged by size:
803 764 164 162
AhdI GACnn_n'nnGTC
Cuts at: 0 449 1893
Size: 449 1444
AlwNI CAG_nnn'CTG
Cuts at: 0 366 765 1155 1893
Size: 366 399 390 738
Fragments arranged by size:
738 399 390 366
ApaLI G'TGCA_C
Cuts at: 0 1531 1893
Size: 1531 362
ApoI r'AATT_y
Cuts at: 0 86 1204 1893
Size: 86 1118 689
Fragments arranged by size:
1118 689 86
AvaI C'yCGr_G
Cuts at: 0 1111 1664 1893
Size: 1111 553 229
Fragments arranged by size:
1111 553 229
BanI G'GyrC_C
Cuts at: 0 1321 1893
Size: 1321 572
BanII G_rGCy'C
Cuts at: 0 98 314 1893
Size: 98 216 1579
Fragments arranged by size:
1579 216 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 448 1078 1358 1893
Size: 448 630 280 535
Fragments arranged by size:
630 535 448 280
BbvCI CC'TCA_GC
Cuts at: 0 659 1893
Size: 659 1234
BciVI GTATCCnnnnn_n'
Cuts at: 0 438 1153 1893
Size: 438 715 740
Fragments arranged by size:
740 715 438
BglI GCCn_nnn'nGGC
Cuts at: 0 15 1893
Size: 15 1878
Bme1580I G_kGCm'C
Cuts at: 0 1535 1893
Size: 1535 358
BmrI ACTGGGnnnn_n'
Cuts at: 0 426 1893
Size: 426 1467
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1653 1893
Size: 1653 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 328 1287 1893
Size: 112 216 959 606
Fragments arranged by size:
959 606 216 112
Bpu10I CC'TnA_GC
Cuts at: 0 659 1893
Size: 659 1234
BsaI GGTCTCn'nnnn_
Cuts at: 0 37 1757 1893
Size: 37 1720 136
Fragments arranged by size:
1720 136 37
BsaAI yAC'GTr
Cuts at: 0 1282 1893
Size: 1282 611
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 580 811 1240 1855 1893
Size: 580 231 429 615 38
Fragments arranged by size:
615 580 429 231 38
BseYI C'CCAG_C
Cuts at: 0 109 935 1893
Size: 109 826 958
Fragments arranged by size:
958 826 109
BsiHKAI G_wGCw'C
Cuts at: 0 98 1164 1278 1535 1893
Size: 98 1066 114 257 358
Fragments arranged by size:
1066 358 257 114 98
BsmBI CGTCTCn'nnnn_
Cuts at: 0 811 1893
Size: 811 1082
Bsp1286I G_dGCh'C
Cuts at: 0 98 314 1164 1278 1535 1893
Size: 98 216 850 114 257 358
Fragments arranged by size:
850 358 257 216 114 98
BspHI T'CATG_A
Cuts at: 0 1344 1626 1893
Size: 1344 282 267
Fragments arranged by size:
1344 282 267
BsrBI CCG'CTC
Cuts at: 0 416 1893
Size: 416 1477
BsrDI GCAATG_nn'
Cuts at: 0 1343 1893
Size: 1343 550
BsrGI T'GTAC_A
Cuts at: 0 524 1893
Size: 524 1369
BssSI C'ACGA_G
Cuts at: 0 718 1893
Size: 718 1175
BstYI r'GATC_y
Cuts at: 0 320 1893
Size: 320 1573
Bsu36I CC'TnA_GG
Cuts at: 0 458 1893
Size: 458 1435
BtgI C'CryG_G
Cuts at: 0 821 1646 1893
Size: 821 825 247
Fragments arranged by size:
825 821 247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 928 1893
Size: 928 965
BtsI GCAGTG_nn'
Cuts at: 0 1535 1893
Size: 1535 358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 803 838 1893
Size: 803 35 1055
Fragments arranged by size:
1055 803 35
DraI TTT'AAA
Cuts at: 0 1209 1893
Size: 1209 684
DraIII CAC_nnn'GTG
Cuts at: 0 1282 1893
Size: 1282 611
EarI CTCTTCn'nnn_
Cuts at: 0 389 740 995 1489 1755 1893
Size: 389 351 255 494 266 138
Fragments arranged by size:
494 389 351 266 255 138
EcoICRI GAG'CTC
Cuts at: 0 96 1893
Size: 96 1797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 328 764 926 1090 1287 1893
Size: 112 216 436 162 164 197 606
Fragments arranged by size:
606 436 216 197 164 162 112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 748 1405 1569 1893
Size: 748 657 164 324
Fragments arranged by size:
748 657 324 164
FspI TGC'GCA
Cuts at: 0 980 1893
Size: 980 913
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 435 467 568 600 874 906 978 1010
Size: 435 32 101 32 274 32 72 32
Cuts at: 1010 1893
Size: 883
Fragments arranged by size:
883 435 274 101 72 32 32 32
32
HincII GTy'rAC
Cuts at: 0 80 1893
Size: 80 1813
KpnI G_GTAC'C
Cuts at: 0 1325 1893
Size: 1325 568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1893
Size: 517 1376
MspA1I CmG'CkG
Cuts at: 0 109 306 742 904 1893
Size: 109 197 436 162 989
Fragments arranged by size:
989 436 197 162 109
NcoI C'CATG_G
Cuts at: 0 1646 1893
Size: 1646 247
PasI CC'CwG_GG
Cuts at: 0 11 1893
Size: 11 1882
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 834 1785 1893
Size: 330 504 951 108
Fragments arranged by size:
951 504 330 108
PfoI T'CCnGG_A
Cuts at: 0 35 1893
Size: 35 1858
PmlI CAC'GTG
Cuts at: 0 1282 1893
Size: 1282 611
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1765 1797 1893
Size: 1765 32 96
Fragments arranged by size:
1765 96 32
PvuII CAG'CTG
Cuts at: 0 109 306 742 904 1893
Size: 109 197 436 162 989
Fragments arranged by size:
989 436 197 162 109
SacI G_AGCT'C
Cuts at: 0 98 1893
Size: 98 1795
SapI GCTCTTCn'nnn_
Cuts at: 0 740 995 1893
Size: 740 255 898
Fragments arranged by size:
898 740 255
ScaI AGT'ACT
Cuts at: 0 1783 1893
Size: 1783 110
SfcI C'TryA_G
Cuts at: 0 380 698 1147 1240 1408 1893
Size: 380 318 449 93 168 485
Fragments arranged by size:
485 449 380 318 168 93
SmaI CCC'GGG
Cuts at: 0 1666 1893
Size: 1666 227
SmlI C'TyrA_G
Cuts at: 0 1111 1893
Size: 1111 782
StuI AGG'CCT
Cuts at: 0 1888 1893
Size: 1888 5
StyI C'CwwG_G
Cuts at: 0 283 1646 1871 1893
Size: 283 1363 225 22
Fragments arranged by size:
1363 283 225 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 264 391 1893
Size: 264 127 1502
Fragments arranged by size:
1502 264 127
TatI w'GTAC_w
Cuts at: 0 524 1781 1893
Size: 524 1257 112
Fragments arranged by size:
1257 524 112
XhoI C'TCGA_G
Cuts at: 0 1111 1893
Size: 1111 782
XmaI C'CCGG_G
Cuts at: 0 1664 1893
Size: 1664 229
XmnI GAAnn'nnTTC
Cuts at: 0 1831 1893
Size: 1831 62
Enzymes that do cut:
Acc65I AcuI AhdI AlwNI ApaLI ApoI AvaI BanI
BanII BbsI BbvCI BciVI BglI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaAI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspHI BsrBI BsrDI BsrGI BssSI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII EarI EcoICRI
Eco57MI FalI FspI Hin4I HincII KpnI MmeI MspA1I
NcoI PasI PflMI PfoI PmlI PsrI PvuII SacI
SapI ScaI SfcI SmaI SmlI StuI StyI TaqII
TatI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BclI BfrBI BglII BlpI
BmgBI BmtI BpuEI BsaBI BsaHI BsaWI BsaXI BsgI
BsiEI BsiWI BsmI BspEI BspMI BsrFI BssHII BstAPI
BstBI BstEII BstXI BstZ17I ClaI DrdI EaeI EagI
EciI EcoNI EcoO109I EcoRI EcoRV FseI FspAI HaeII
HindIII HpaI KasI MfeI MluI MscI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XcmI ZraI