(Circular) (Six-base) MAPSORT of: pFN21ASDB0005.seq Check: 6214 from: 1 to: 8542 pFN21ASDB0005 8542 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5343 6352 278 Size: 53 83 186 936 3807 1009 2468 Fragments arranged by size: 3807 2468 1009 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2748 4869 5835 5885 1937 Size: 811 2121 966 50 4594 Fragments arranged by size: 4594 2121 966 811 50 Acc65I G'GTAC_C Cuts at: 5863 5863 Size: 8542 AclI AA'CG_TT Cuts at: 1563 6669 7042 1563 Size: 5106 373 3063 Fragments arranged by size: 5106 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2810 4025 4172 4484 4507 5345 5426 6605 Size: 1178 1215 147 312 23 838 81 1179 Cuts at: 6605 7989 8257 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1215 1179 1178 838 312 268 147 81 23 AfeI AGC'GCT Cuts at: 2132 2180 2132 Size: 48 8494 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8345 AflIII A'CryG_T Cuts at: 1203 4554 7441 8418 1203 Size: 3351 2887 977 1327 Fragments arranged by size: 3351 2887 1327 977 AgeI A'CCGG_T Cuts at: 7373 7373 Size: 8542 AhdI GACnn_n'nnGTC Cuts at: 5725 7271 5725 Size: 1546 6996 AleI CACnn'nnGTG Cuts at: 1166 3233 1166 Size: 2067 6475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2111 4032 1184 Size: 927 1921 5694 Fragments arranged by size: 5694 1921 927 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3023 3055 4061 4093 3023 Size: 32 1006 32 7472 Fragments arranged by size: 7472 1006 32 32 AlwNI CAG_nnn'CTG Cuts at: 2125 3772 4150 4666 4784 4936 5050 5064 5323 Size: 1647 378 516 118 152 114 14 259 Cuts at: 5323 5758 6285 7857 2125 Size: 435 527 1572 2810 Fragments arranged by size: 2810 1647 1572 527 516 435 378 259 152 118 114 14 ApaI G_GGCC'C Cuts at: 2906 4189 2906 Size: 1283 7259 ApaLI G'TGCA_C Cuts at: 4529 6599 7755 4529 Size: 2070 1156 5316 Fragments arranged by size: 5316 2070 1156 ApoI r'AATT_y Cuts at: 1493 4904 5851 6077 6229 8338 1493 Size: 3411 947 226 152 2109 1697 Fragments arranged by size: 3411 2109 1697 947 226 152 AseI AT'TA_AT Cuts at: 160 1032 7096 160 Size: 872 6064 1606 Fragments arranged by size: 6064 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8542 AvaI C'yCGr_G Cuts at: 1870 1943 2292 2360 2405 2423 2525 2683 3457 Size: 73 349 68 45 18 102 158 774 Cuts at: 3457 5429 5867 1870 Size: 1972 438 4545 Fragments arranged by size: 4545 1972 774 438 349 158 102 73 68 45 18 BamHI G'GATC_C Cuts at: 2502 3916 5872 6239 2502 Size: 1414 1956 367 4805 Fragments arranged by size: 4805 1956 1414 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2151 2279 3195 3281 3366 Size: 325 217 627 364 128 916 86 85 Cuts at: 3366 4357 5221 5863 7318 618 Size: 991 864 642 1455 1842 Fragments arranged by size: 1842 1455 991 916 864 642 627 364 325 217 128 86 85 BanII G_rGCy'C Cuts at: 1391 2082 2292 2361 2684 2906 3466 3681 3714 Size: 691 210 69 323 222 560 215 33 Cuts at: 3714 4189 4749 4928 5436 5861 1391 Size: 475 560 179 508 425 4072 Fragments arranged by size: 4072 691 560 560 508 475 425 323 222 215 210 179 69 33 BbeI G_GCGC'C Cuts at: 2155 2283 3285 2155 Size: 128 1002 7412 Fragments arranged by size: 7412 1002 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4198 5167 928 Size: 971 2299 969 4303 Fragments arranged by size: 4303 2299 971 969 BbvCI CC'TCA_GC Cuts at: 3419 3419 Size: 8542 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2418 2452 2854 2888 3288 3322 5062 Size: 34 481 34 402 34 400 34 1740 Cuts at: 5062 5096 6733 6767 1903 Size: 34 1637 34 3678 Fragments arranged by size: 3678 1740 1637 481 402 400 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3320 4935 6434 7644 3320 Size: 1615 1499 1210 4218 Fragments arranged by size: 4218 1615 1499 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8542 BfrBI ATG'CAT Cuts at: 2699 2699 Size: 8542 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2474 2833 4542 Size: 107 122 71 631 605 802 359 1709 Cuts at: 4542 7153 136 Size: 2611 1525 Fragments arranged by size: 2611 1709 1525 802 631 605 359 122 107 71 BglII A'GATC_T Cuts at: 3258 4392 8537 3258 Size: 1134 4145 3263 Fragments arranged by size: 4145 3263 1134 BlpI GC'TnA_GC Cuts at: 4884 5959 4884 Size: 1075 7467 Bme1580I G_kGCm'C Cuts at: 1790 2906 4189 4344 4362 4533 5226 6603 7759 Size: 1116 1283 155 18 171 693 1377 1156 Cuts at: 7759 1790 Size: 2573 Fragments arranged by size: 2573 1377 1283 1156 1116 693 171 155 18 BmgBI CAC'GTC Cuts at: 1318 4204 5383 1318 Size: 2886 1179 4477 Fragments arranged by size: 4477 2886 1179 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6273 7231 442 Size: 416 53 83 399 24 4856 958 1753 Fragments arranged by size: 4856 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3549 1056 Size: 2493 6049 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2116 3073 2116 Size: 957 7585 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2177 2864 3025 3488 3593 4352 4673 Size: 908 30 687 161 463 105 759 321 Cuts at: 4673 4841 4940 5798 7202 1239 Size: 168 99 858 1404 2579 Fragments arranged by size: 2579 1404 908 858 759 687 463 321 168 161 105 99 30 Bpu10I CC'TnA_GC Cuts at: 2483 3419 3563 3675 2483 Size: 936 144 112 7350 Fragments arranged by size: 7350 936 144 112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4226 6667 7532 7830 4226 Size: 2441 865 298 4938 Fragments arranged by size: 4938 2441 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3460 3647 7205 882 Size: 617 1961 187 3558 2219 Fragments arranged by size: 3558 2219 1961 617 187 BsaAI yAC'GTr Cuts at: 493 1204 2554 5839 6333 493 Size: 711 1350 3285 494 2702 Fragments arranged by size: 3285 2702 1350 711 494 BsaBI GATnn'nnATC Cuts at: 4391 8536 4391 Size: 4145 4397 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2152 2280 2535 2745 Size: 53 83 186 936 619 128 255 210 Cuts at: 2745 2766 2808 3240 3282 4050 5340 6349 6731 Size: 21 42 432 42 768 1290 1009 382 Cuts at: 6731 7366 275 Size: 635 1451 Fragments arranged by size: 1451 1290 1009 936 768 635 619 432 382 255 210 186 128 83 53 42 42 21 BsaWI w'CCGG_w Cuts at: 5297 6975 7373 7647 7794 8205 5297 Size: 1678 398 274 147 411 5634 Fragments arranged by size: 5634 1678 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2267 2297 3005 3023 3035 Size: 30 165 30 864 30 708 18 12 Cuts at: 3035 3053 3386 3416 4472 4502 5726 5756 1178 Size: 18 333 30 1056 30 1224 30 3964 Fragments arranged by size: 3964 1224 1056 864 708 333 165 30 30 30 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2369 2716 3049 3062 3368 3394 3566 3991 Size: 1183 347 333 13 306 26 172 425 Cuts at: 3991 4106 4382 4558 5264 5288 1186 Size: 115 276 176 706 24 4440 Fragments arranged by size: 4440 1183 706 425 347 333 306 276 176 172 115 26 24 13 BseYI C'CCAG_C Cuts at: 2667 3784 4511 5696 7745 8241 2667 Size: 1117 727 1185 2049 496 2968 Fragments arranged by size: 2968 2049 1185 1117 727 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3746 4216 4605 4798 5606 1163 Size: 573 2010 470 389 193 808 4099 Fragments arranged by size: 4099 2010 808 573 470 389 193 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2455 5992 6753 7781 1540 Size: 275 186 454 3537 761 1028 2301 Fragments arranged by size: 3537 2301 1028 761 454 275 186 BsiHKAI G_wGCw'C Cuts at: 2927 3681 3983 4140 4533 5003 6603 6688 7759 Size: 754 302 157 393 470 1600 85 1071 Cuts at: 7759 2927 Size: 3710 Fragments arranged by size: 3710 1600 1071 754 470 393 302 157 85 BsmI GAATG_Cn' Cuts at: 4704 6053 6146 4704 Size: 1349 93 7100 Fragments arranged by size: 7100 1349 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3288 3288 Size: 8542 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2292 2361 2684 2906 2927 3466 Size: 399 292 210 69 323 222 21 539 Cuts at: 3466 3681 3714 3983 4140 4189 4344 4362 4533 Size: 215 33 269 157 49 155 18 171 Cuts at: 4533 4749 4928 5003 5226 5436 5861 6603 6688 Size: 216 179 75 223 210 425 742 85 Cuts at: 6688 7759 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 539 425 399 323 292 269 223 222 216 215 210 210 179 171 157 155 85 75 69 49 33 21 18 BspEI T'CCGG_A Cuts at: 8205 8205 Size: 8542 BspHI T'CATG_A Cuts at: 6431 8161 6431 Size: 1730 6812 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2205 2952 4230 5529 5556 5897 844 Size: 1361 747 1278 1299 27 341 3489 Fragments arranged by size: 3489 1361 1299 1278 747 341 27 BsrBI CCG'CTC Cuts at: 1394 1823 2500 2908 3126 4254 4297 6429 1394 Size: 429 677 408 218 1128 43 2132 3507 Fragments arranged by size: 3507 2132 1128 677 429 408 218 43 BsrDI GCAATG_nn' Cuts at: 65 1248 7037 7211 65 Size: 1183 5789 174 1396 Fragments arranged by size: 5789 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 3198 3363 3628 4140 7186 7373 Size: 110 425 963 165 265 512 3046 187 Cuts at: 7373 1700 Size: 2869 Fragments arranged by size: 3046 2869 963 512 425 265 187 165 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8542 BssHII G'CGCG_C Cuts at: 1925 2257 1925 Size: 332 8210 BssSI C'ACGA_G Cuts at: 2573 4675 6602 7614 2573 Size: 2102 1927 1012 3501 Fragments arranged by size: 3501 2102 1927 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2206 5050 794 Size: 1412 2844 4286 Fragments arranged by size: 4286 2844 1412 BstBI TT'CG_AA Cuts at: 2007 7346 2007 Size: 5339 3203 BstEII G'GTnAC_C Cuts at: 1184 5197 1184 Size: 4013 4529 BstXI CCAn_nnnn'nTGG Cuts at: 2650 3235 3784 3832 2650 Size: 585 549 48 7360 Fragments arranged by size: 7360 585 549 48 BstYI r'GATC_y Cuts at: 1972 2502 3258 3687 3916 4303 4392 4977 5872 Size: 530 756 429 229 387 89 585 895 Cuts at: 5872 6239 6624 6641 8093 8179 8191 8537 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 895 756 585 530 429 387 385 367 346 229 89 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4937 4937 Size: 8542 BtgI C'CryG_G Cuts at: 513 1065 2022 2515 3289 3825 4650 5307 5634 Size: 552 957 493 774 536 825 657 327 Cuts at: 5634 513 Size: 3421 Fragments arranged by size: 3421 957 825 774 657 552 536 493 327 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2257 3393 5065 5377 5461 487 Size: 683 56 1031 1136 1672 312 84 3568 Fragments arranged by size: 3568 1672 1136 1031 683 312 84 56 BtsI GCAGTG_nn' Cuts at: 3188 3202 3578 3737 4344 4490 6058 6852 6872 Size: 14 376 159 607 146 1568 794 20 Cuts at: 6872 3188 Size: 4858 Fragments arranged by size: 4858 1568 794 607 376 159 146 20 14 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8542 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5623 5658 529 Size: 35 5059 35 3413 Fragments arranged by size: 5059 3413 35 35 DraI TTT'AAA Cuts at: 5846 5846 Size: 8542 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7549 809 Size: 507 6233 1802 Fragments arranged by size: 6233 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2518 2834 Size: 54 1425 50 275 18 381 307 316 Cuts at: 2834 3064 3774 5304 5989 6878 8507 8 Size: 230 710 1530 685 889 1629 43 Fragments arranged by size: 1629 1530 1425 889 710 685 381 316 307 275 230 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5989 1537 Size: 275 4177 4090 Fragments arranged by size: 4177 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2219 3719 3767 4421 5138 5444 6472 1353 Size: 866 1500 48 654 717 306 1028 3423 Fragments arranged by size: 3423 1500 1028 866 717 654 306 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2546 7129 7503 7649 387 Size: 1064 138 957 4583 374 146 1280 Fragments arranged by size: 4583 1280 1064 957 374 146 138 EcoICRI GAG'CTC Cuts at: 3679 3679 Size: 8542 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2177 2810 2864 3025 3488 3593 Size: 393 515 30 633 54 161 463 105 Cuts at: 3593 4025 4172 4352 4484 4507 4673 4841 4940 Size: 432 147 180 132 23 166 168 99 Cuts at: 4940 5345 5426 5798 6605 7202 7989 8257 1239 Size: 405 81 372 807 597 787 268 1524 Fragments arranged by size: 1524 807 787 633 597 515 463 432 405 393 372 268 180 168 166 161 147 132 105 99 81 54 30 23 EcoNI CCTnn'n_nnAGG Cuts at: 2159 2588 5537 2159 Size: 429 2949 5164 Fragments arranged by size: 5164 2949 429 EcoO109I rG'GnC_Cy Cuts at: 2269 2459 2706 2826 2902 2964 3526 4185 5047 Size: 190 247 120 76 62 562 659 862 Cuts at: 5047 2269 Size: 5764 Fragments arranged by size: 5764 862 659 562 247 190 120 76 62 EcoRI G'AATT_C Cuts at: 5851 6229 5851 Size: 378 8164 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4177 5350 5431 4177 Size: 1173 81 7288 Fragments arranged by size: 7288 1173 81 FspI TGC'GCA Cuts at: 2915 7048 2915 Size: 4133 4409 HaeII r_GCGC'y Cuts at: 1678 2134 2155 2182 2283 2324 2483 2947 3285 Size: 456 21 27 101 41 159 464 338 Cuts at: 3285 3364 5263 7689 1678 Size: 79 1899 2426 2531 Fragments arranged by size: 2531 2426 1899 464 456 338 159 101 79 41 27 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2267 2299 3005 3037 3044 Size: 32 135 32 695 32 706 32 7 Cuts at: 3044 3076 3386 3418 4472 4504 4721 4753 4789 Size: 32 310 32 1054 32 217 32 36 Cuts at: 4789 4821 5711 5726 5743 5758 5799 5831 7183 Size: 32 890 15 17 15 41 32 1352 Cuts at: 7183 7215 7257 7289 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1352 1054 890 706 695 310 217 135 42 41 36 32 32 32 32 32 32 32 32 32 32 32 32 17 15 15 7 HincII GTy'rAC Cuts at: 669 1657 1938 2596 2749 5886 6132 669 Size: 988 281 658 153 3137 246 3079 Fragments arranged by size: 3137 3079 988 658 281 246 153 HindIII A'AGCT_T Cuts at: 748 4633 748 Size: 3885 4657 HpaI GTT'AAC Cuts at: 6132 6132 Size: 8542 KasI G'GCGC_C Cuts at: 2151 2279 3281 2151 Size: 128 1002 7412 Fragments arranged by size: 7412 1002 128 KpnI G_GTAC'C Cuts at: 5867 5867 Size: 8542 MfeI C'AATT_G Cuts at: 6141 6141 Size: 8542 MluI A'CGCG_T Cuts at: 8418 8418 Size: 8542 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2514 2592 3944 4458 7656 7840 1305 Size: 1209 78 1352 514 3198 184 2007 Fragments arranged by size: 3198 2007 1352 1209 514 184 78 MscI TGG'CCA Cuts at: 10 64 1489 1832 2213 2520 3066 3776 5306 Size: 54 1425 343 381 307 546 710 1530 Cuts at: 5306 10 Size: 3246 Fragments arranged by size: 3246 1530 1425 710 546 381 343 307 54 MslI CAynn'nnrTG Cuts at: 518 1166 2648 3233 3372 3830 4121 5084 6500 Size: 648 1482 585 139 458 291 963 1416 Cuts at: 6500 6859 7018 518 Size: 359 159 2042 Fragments arranged by size: 2042 1482 1416 963 648 585 458 359 291 159 139 MspA1I CmG'CkG Cuts at: 1606 2739 3611 3748 3784 4147 4711 4883 5035 Size: 1133 872 137 36 363 564 172 152 Cuts at: 5035 5724 5776 5958 6635 7783 8028 1606 Size: 689 52 182 677 1148 245 2120 Fragments arranged by size: 2120 1148 1133 872 689 677 564 363 245 182 172 152 137 52 36 NaeI GCC'GGC Cuts at: 3365 3365 Size: 8542 NarI GG'CG_CC Cuts at: 2152 2280 3282 2152 Size: 128 1002 7412 Fragments arranged by size: 7412 1002 128 NcoI C'CATG_G Cuts at: 513 1065 2022 2515 3289 3825 513 Size: 552 957 493 774 536 5230 Fragments arranged by size: 5230 957 774 552 536 493 NdeI CA'TA_TG Cuts at: 387 387 Size: 8542 NgoMIV G'CCGG_C Cuts at: 3363 3363 Size: 8542 NheI G'CTAG_C Cuts at: 1052 3545 1052 Size: 2493 6049 NotI GC'GGCC_GC Cuts at: 5989 5989 Size: 8542 NsiI A_TGCA'T Cuts at: 2701 2701 Size: 8542 NspI r_CATG'y Cuts at: 1132 1229 3109 5515 5900 7445 1132 Size: 97 1880 2406 385 1545 2229 Fragments arranged by size: 2406 2229 1880 1545 385 97 PasI CC'CwG_GG Cuts at: 1348 2629 1348 Size: 1281 7261 PciI A'CATG_T Cuts at: 7441 7441 Size: 8542 PflMI CCAn_nnn'nTGG Cuts at: 1108 3772 4321 5245 1108 Size: 2664 549 924 4405 Fragments arranged by size: 4405 2664 924 549 PfoI T'CCnGG_A Cuts at: 2067 3111 4605 2067 Size: 1044 1494 6004 Fragments arranged by size: 6004 1494 1044 PmeI GTTT'AAAC Cuts at: 5846 5846 Size: 8542 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3023 3055 6611 6643 8124 8156 3023 Size: 32 3556 32 1481 32 3409 Fragments arranged by size: 3556 3409 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2459 2964 3526 2459 Size: 505 562 7475 Fragments arranged by size: 7475 562 505 PshAI GACnn'nnGTC Cuts at: 1613 4111 4865 5171 1613 Size: 2498 754 306 4984 Fragments arranged by size: 4984 2498 754 306 PsiI TTA'TAA Cuts at: 6112 6112 Size: 8542 PspOMI G'GGCC_C Cuts at: 2902 4185 2902 Size: 1283 7259 PspXI vC'TCGA_Gb Cuts at: 2683 2683 Size: 8542 PstI C_TGCA'G Cuts at: 830 2202 3020 3343 4047 4662 5055 5775 5894 Size: 1372 818 323 704 615 393 720 119 Cuts at: 5894 830 Size: 3478 Fragments arranged by size: 3478 1372 818 720 704 615 393 323 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8542 PvuII CAG'CTG Cuts at: 2739 3784 4147 4883 5035 5724 5776 2739 Size: 1045 363 736 152 689 52 5505 Fragments arranged by size: 5505 1045 736 689 363 152 52 RsrII CG'GwC_CG Cuts at: 2614 2614 Size: 8542 SacI G_AGCT'C Cuts at: 3681 3681 Size: 8542 SalI G'TCGA_C Cuts at: 1936 2747 5884 1936 Size: 811 3137 4594 Fragments arranged by size: 4594 3137 811 SanDI GG'GwC_CC Cuts at: 2459 2459 Size: 8542 SapI GCTCTTCn'nnn_ Cuts at: 2219 3719 2219 Size: 1500 7042 SbfI CC_TGCA'GG Cuts at: 5055 5894 5055 Size: 839 7703 ScaI AGT'ACT Cuts at: 6790 8502 6790 Size: 1712 6830 SfcI C'TryA_G Cuts at: 826 1045 2198 3016 3339 4043 4658 4870 5051 Size: 219 1153 818 323 704 615 212 181 Cuts at: 5051 5771 5890 7025 7706 7897 826 Size: 720 119 1135 681 191 1471 Fragments arranged by size: 1471 1153 1135 818 720 704 681 615 323 219 212 191 181 119 SfiI GGCCn_nnn'nGGCC Cuts at: 2833 2833 Size: 8542 SfoI GGC'GCC Cuts at: 2153 2281 3283 2153 Size: 128 1002 7412 Fragments arranged by size: 7412 1002 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8542 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8542 SmaI CCC'GGG Cuts at: 1872 2407 2425 2527 5869 1872 Size: 535 18 102 3342 4545 Fragments arranged by size: 4545 3342 535 102 18 SmlI C'TyrA_G Cuts at: 820 1017 1943 2683 4241 6646 7547 7809 820 Size: 197 926 740 1558 2405 901 262 1553 Fragments arranged by size: 2405 1558 1553 926 901 740 262 197 SnaBI TAC'GTA Cuts at: 493 5839 6333 493 Size: 5346 494 2702 Fragments arranged by size: 5346 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8542 SphI G_CATG'C Cuts at: 1132 5515 5900 1132 Size: 4383 385 3774 Fragments arranged by size: 4383 3774 385 SspI AAT'ATT Cuts at: 5 52 6466 5 Size: 47 6414 2081 Fragments arranged by size: 6414 2081 47 StuI AGG'CCT Cuts at: 1517 2141 1517 Size: 624 7918 StyI C'CwwG_G Cuts at: 513 1065 2022 2214 2515 2831 3289 3825 3912 Size: 552 957 192 301 316 458 536 87 Cuts at: 3912 4983 5217 5730 5981 513 Size: 1071 234 513 251 3074 Fragments arranged by size: 3074 1071 957 552 536 513 458 316 301 251 234 192 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3776 5101 6569 6586 6739 6924 7369 Size: 1255 2196 1325 1468 17 153 185 445 Cuts at: 7369 325 Size: 1498 Fragments arranged by size: 2196 1498 1468 1325 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4809 5412 5763 Size: 274 80 33 51 1443 2832 603 351 Cuts at: 5763 6788 8500 96 Size: 1025 1712 138 Fragments arranged by size: 2832 1712 1443 1025 603 351 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4056 4088 8502 8534 4056 Size: 32 4414 32 4064 Fragments arranged by size: 4414 4064 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8542 XbaI T'CTAG_A Cuts at: 5878 5878 Size: 8542 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2152 3934 4351 4781 2152 Size: 1782 417 430 5913 Fragments arranged by size: 5913 1782 430 417 XhoI C'TCGA_G Cuts at: 1943 2683 1943 Size: 740 7802 XmaI C'CCGG_G Cuts at: 1870 2405 2423 2525 5867 1870 Size: 535 18 102 3342 4545 Fragments arranged by size: 4545 3342 535 102 18 XmnI GAAnn'nnTTC Cuts at: 2546 4625 6671 2546 Size: 2079 2046 4417 Fragments arranged by size: 4417 2079 2046 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5341 6350 276 Size: 53 83 186 936 3807 1009 2468 Fragments arranged by size: 3807 2468 1009 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI PacI PmlI PsrI SacII SexAI SrfI SwaI