Restriction map for
pFN21ASDB0004
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# NO CUT ENZYMES ( 84 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AhdI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsaI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrDI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HindIII HpaI KpnI MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SfuI SnaBI SpeI SspI SwaI
Tru9I Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 2647 GACGTC GACGT^C
AgeI 771 ACCGGT A^CCGGT
Aor51HI 2448 AGCGCT AGC^GCT
AspI 2335 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 527 GGATCC G^GATCC
BsaWI 771 ACCGGT [AT]^CCGG[AT]
BsmAI 977 GAGAC GTCTC(1/5)
Bsp1407I 2801 TGTACA T^GTACA
BsrGI 2801 TGTACA T^GTACA
BssSI 2899 CACGAG CACGAG(-5/-1)
BstYI 527 GGATCC [GA]^GATC[CT]
DraIII 580 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2448 AGCGCT AGC^GCT
EcoNI 174 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 51 GGCCGGCC GGCCGG^CC
HincII 2017 GTTGAC GT[CT]^[GA]AC
HindII 2017 GTTGAC GT[CT]^[GA]AC
MflI 527 GGATCC [GA]^GATC[CT]
PaeR7I 2808 CTCGAG C^TCGAG
PflFI 2335 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 771 ACCGGT A^CCGGT
PshAI 1190 GACGGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2284 GAGCTC GAGCT^C
SapI 2487 GCTCTTC GCTCTTC(1/4)
SbfI 1632 CCTGCAGG CCTGCA^GG
SfaNI 2546 GCATC GCATC(5/9)
SmlI 2808 CTCGAG C^T[CT][GA]AG
Sse8387I 1632 CCTGCAGG CCTGCA^GG
SspBI 2801 TGTACA T^GTACA
TfiI 2113 GATTC G^A[AT]TC
Tth111I 2335 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2808 CTCGAG C^TCGAG
XhoII 527 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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Alw44I 1044 GTGCAC G^TGCAC
2374 GTGCAC
AlwI 528 GATCC GGATC(4/5)
527 GGATC
ApaLI 1044 GTGCAC G^TGCAC
2374 GTGCAC
BbeI 1741 GGCGCC GGCGC^C
1957 GGCGCC
BbsI 1573 GTCTTC GAAGAC(2/6)
2314 GTCTTC
BbvCI 1312 CCTCAGC CCTCAGC(-5/-2)
2586 CCTCAGC
BfaI 856 CTAG C^TAG
3109 CTAG
BlpI 120 GCTCAGC GC^T[ACGT]AGC
1986 GCTCAGC
Bpu1102I 120 GCTCAGC GC^T[ACGT]AGC
1986 GCTCAGC
BpuAI 1573 GTCTTC GAAGAC(2/6)
2314 GTCTTC
BssHII 2429 GCGCGC G^CGCGC
2619 GCGCGC
BstXI 1113 CCAGGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1781 CCACGGTGCTGG
CelII 120 GCTCAGC GC^T[ACGT]AGC
1986 GCTCAGC
KasI 1741 GGCGCC G^GCGCC
1957 GGCGCC
KspI 1508 CCGCGG CCGC^GG
2886 CCGCGG
MaeI 856 CTAG C^TAG
3109 CTAG
NarI 1741 GGCGCC GG^CGCC
1957 GGCGCC
PleI 2353 GACTC GAGTC(4/5)
1492 GAGTC
SacII 1508 CCGCGG CCGC^GG
2886 CCGCGG
SfiI 1203 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2255 GGCCACACTGGCC
SfoI 1741 GGCGCC GGC^GCC
1957 GGCGCC
SgrAI 770 CACCGGTG C[GA]^CCGG[CT]G
1953 CACCGGCG
SphI 1367 GCATGC GCATG^C
2148 GCATGC
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# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvI BcnI BglI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfcI SmaI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XmaCI XmaI