(Circular) (Six-base) MAPSORT of: pFN21ASDB0004.seq Check: 1890 from: 1 to: 7987 pFN21ASDB0004 7987 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4305 4563 4305 Size: 258 7729 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4674 5797 278 Size: 53 83 186 936 3138 1123 2468 Fragments arranged by size: 3138 2468 1123 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5330 1937 Size: 3393 4594 Acc65I G'GTAC_C Cuts at: 5308 5308 Size: 7987 AclI AA'CG_TT Cuts at: 1563 6114 6487 1563 Size: 4551 373 3063 Fragments arranged by size: 4551 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2701 3583 6050 7434 7702 1632 Size: 1069 882 2467 1384 268 1917 Fragments arranged by size: 2467 1917 1384 1069 882 268 AfeI AGC'GCT Cuts at: 4473 4473 Size: 7987 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7790 AflIII A'CryG_T Cuts at: 1203 6886 7863 1203 Size: 5683 977 1327 Fragments arranged by size: 5683 1327 977 AgeI A'CCGG_T Cuts at: 2794 6818 2794 Size: 4024 3963 AhdI GACnn_n'nnGTC Cuts at: 6716 6716 Size: 7987 AleI CACnn'nnGTG Cuts at: 1166 2495 4299 1166 Size: 1329 1804 4854 Fragments arranged by size: 4854 1804 1329 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7987 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4073 4105 4073 Size: 32 7955 AlwNI CAG_nnn'CTG Cuts at: 2508 3195 3207 3343 3568 3664 5113 5205 5730 Size: 687 12 136 225 96 1449 92 525 Cuts at: 5730 7302 2508 Size: 1572 3193 Fragments arranged by size: 3193 1572 1449 687 525 225 136 96 92 12 ApaI G_GGCC'C Cuts at: 2528 2681 3513 5271 2528 Size: 153 832 1758 5244 Fragments arranged by size: 5244 1758 832 153 ApaLI G'TGCA_C Cuts at: 3067 4397 6044 7200 3067 Size: 1330 1647 1156 3854 Fragments arranged by size: 3854 1647 1330 1156 ApoI r'AATT_y Cuts at: 1493 5296 5522 5674 7783 1493 Size: 3803 226 152 2109 1697 Fragments arranged by size: 3803 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6541 160 Size: 872 5509 1606 Fragments arranged by size: 5509 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7987 AvaI C'yCGr_G Cuts at: 1870 1943 2636 2854 2971 3251 3259 3506 3512 Size: 73 693 218 117 280 8 247 6 Cuts at: 3512 4783 4831 5312 1870 Size: 1271 48 481 4545 Fragments arranged by size: 4545 1271 693 481 280 247 218 117 73 48 8 6 BamHI G'GATC_C Cuts at: 2550 5317 5684 2550 Size: 2767 367 4853 Fragments arranged by size: 4853 2767 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2645 2797 2939 3764 3980 Size: 325 217 627 858 152 142 825 216 Cuts at: 3980 4100 4208 4293 4742 5308 6763 618 Size: 120 108 85 449 566 1455 1842 Fragments arranged by size: 1842 1455 858 825 627 566 449 325 217 216 152 142 120 108 85 BanII G_rGCy'C Cuts at: 1391 2313 2376 2451 2528 2681 2845 2855 3153 Size: 922 63 75 77 153 164 10 298 Cuts at: 3153 3513 4038 4311 4854 4977 5271 5306 1391 Size: 360 525 273 543 123 294 35 4072 Fragments arranged by size: 4072 922 543 525 360 298 294 273 164 153 123 77 75 63 35 10 BbeI G_GCGC'C Cuts at: 3768 3984 3768 Size: 216 7771 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3589 4330 928 Size: 971 1690 741 4585 Fragments arranged by size: 4585 1690 971 741 BbvCI CC'TCA_GC Cuts at: 3336 4610 3336 Size: 1274 6713 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6178 6212 1903 Size: 34 4241 34 3678 Fragments arranged by size: 4241 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5879 7089 5879 Size: 1210 6777 BclI T'GATC_A Cuts at: 1548 1548 Size: 7987 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3198 3233 3376 Size: 107 122 71 631 605 1526 35 143 Cuts at: 3376 4285 4757 4935 6598 136 Size: 909 472 178 1663 1525 Fragments arranged by size: 1663 1526 1525 909 631 605 472 178 143 122 107 71 35 BglII A'GATC_T Cuts at: 7982 7982 Size: 7987 BlpI GC'TnA_GC Cuts at: 2144 4010 5404 2144 Size: 1866 1394 4727 Fragments arranged by size: 4727 1866 1394 Bme1580I G_kGCm'C Cuts at: 1790 2528 2681 3071 3513 4211 4296 4401 4745 Size: 738 153 390 442 698 85 105 344 Cuts at: 4745 5271 6048 7204 1790 Size: 526 777 1156 2573 Fragments arranged by size: 2573 1156 777 738 698 526 442 390 344 153 105 85 BmgBI CAC'GTC Cuts at: 1318 2605 1318 Size: 1287 6700 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4732 5718 6676 Size: 416 53 83 399 24 3315 986 958 Cuts at: 6676 442 Size: 1753 Fragments arranged by size: 3315 1753 986 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7987 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4510 4510 Size: 7987 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2936 3016 3923 4505 4520 4700 4747 6647 Size: 1697 80 907 582 15 180 47 1900 Cuts at: 6647 1239 Size: 2579 Fragments arranged by size: 2579 1900 1697 907 582 180 80 47 15 Bpu10I CC'TnA_GC Cuts at: 3336 3686 4610 3336 Size: 350 924 6713 Fragments arranged by size: 6713 924 350 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6112 6977 7275 6112 Size: 865 298 6824 Fragments arranged by size: 6824 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6650 882 Size: 617 5151 2219 Fragments arranged by size: 5151 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3397 3618 3798 4813 5020 5284 5778 Size: 711 2193 221 180 1015 207 264 494 Cuts at: 5778 493 Size: 2702 Fragments arranged by size: 2702 2193 1015 711 494 264 221 207 180 BsaBI GATnn'nnATC Cuts at: 7981 7981 Size: 7987 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3765 3981 4335 4539 Size: 53 83 186 936 2232 216 354 204 Cuts at: 4539 4671 4884 5794 6176 6811 275 Size: 132 213 910 382 635 1451 Fragments arranged by size: 2232 1451 936 910 635 382 354 216 213 204 186 132 83 53 BsaWI w'CCGG_w Cuts at: 2794 6420 6818 7092 7239 7650 2794 Size: 3626 398 274 147 411 3131 Fragments arranged by size: 3626 3131 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2616 2646 2991 3021 4073 Size: 30 165 30 1213 30 345 30 1052 Cuts at: 4073 4103 4361 4391 1178 Size: 30 258 30 4774 Fragments arranged by size: 4774 1213 1052 345 258 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2420 2423 2432 2441 2444 2447 3842 Size: 982 252 3 9 9 3 3 1395 Cuts at: 3842 4046 1186 Size: 204 5127 Fragments arranged by size: 5127 1395 982 252 204 9 9 3 3 3 BseYI C'CCAG_C Cuts at: 2460 2662 2680 2781 2811 2859 2864 3188 3351 Size: 202 18 101 30 48 5 324 163 Cuts at: 3351 3667 4085 4439 4863 5127 7190 7686 2460 Size: 316 418 354 424 264 2063 496 2761 Fragments arranged by size: 2761 2063 496 424 418 354 324 316 264 202 163 101 48 30 18 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2041 2164 2468 3110 3566 3777 3902 Size: 573 305 123 304 642 456 211 125 Cuts at: 3902 4445 5000 5017 5042 5084 1163 Size: 543 555 17 25 42 4066 Fragments arranged by size: 4066 642 573 555 543 456 305 304 211 125 123 42 25 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 2076 2595 3716 5276 5437 Size: 275 186 33 42 519 1121 1560 161 Cuts at: 5437 6198 7226 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1560 1121 1028 761 519 275 186 161 42 33 BsiHKAI G_wGCw'C Cuts at: 2093 3071 3104 4311 4401 6048 6133 7204 2093 Size: 978 33 1207 90 1647 85 1071 2876 Fragments arranged by size: 2876 1647 1207 1071 978 90 85 33 BsmI GAATG_Cn' Cuts at: 5498 5591 5498 Size: 93 7894 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2313 2376 2451 2528 2681 2845 Size: 399 303 220 63 75 77 153 164 Cuts at: 2845 2855 3071 3104 3153 3513 4038 4211 4296 Size: 10 216 33 49 360 525 173 85 Cuts at: 4296 4311 4401 4745 4854 4977 5271 5306 6048 Size: 15 90 344 109 123 294 35 742 Cuts at: 6048 6133 7204 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 525 399 360 344 303 294 220 216 173 164 153 123 109 90 85 85 77 75 63 49 35 33 15 10 BspEI T'CCGG_A Cuts at: 7650 7650 Size: 7987 BspHI T'CATG_A Cuts at: 5876 7606 5876 Size: 1730 6257 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2327 4305 4563 4952 5022 5098 5342 844 Size: 1483 1978 258 389 70 76 244 3489 Fragments arranged by size: 3489 1978 1483 389 258 244 76 70 BsrBI CCG'CTC Cuts at: 1394 1823 2441 3712 3730 4489 4918 5239 5874 Size: 429 618 1271 18 759 429 321 635 Cuts at: 5874 1394 Size: 3507 Fragments arranged by size: 3507 1271 759 635 618 429 429 321 18 BsrDI GCAATG_nn' Cuts at: 65 1248 6482 6656 65 Size: 1183 5234 174 1396 Fragments arranged by size: 5234 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2075 2594 2794 2828 2957 3842 3977 Size: 110 265 519 200 34 129 885 135 Cuts at: 3977 5144 5255 6631 6818 1700 Size: 1167 111 1376 187 2869 Fragments arranged by size: 2869 1376 1167 885 519 265 200 187 135 129 111 110 34 BsrGI T'GTAC_A Cuts at: 96 4824 96 Size: 4728 3259 BssHII G'CGCG_C Cuts at: 1925 4452 4642 1925 Size: 2527 190 5270 Fragments arranged by size: 5270 2527 190 BssSI C'ACGA_G Cuts at: 4922 6047 7059 4922 Size: 1125 1012 5850 Fragments arranged by size: 5850 1125 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3207 3568 3664 4944 794 Size: 2413 361 96 1280 3837 Fragments arranged by size: 3837 2413 1280 361 96 BstBI TT'CG_AA Cuts at: 2007 6791 2007 Size: 4784 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7987 BstXI CCAn_nnnn'nTGG Cuts at: 3143 3811 3143 Size: 668 7319 BstYI r'GATC_y Cuts at: 1972 2550 5317 5684 6069 6086 7538 7624 7636 Size: 578 2767 367 385 17 1452 86 12 Cuts at: 7636 7982 1972 Size: 346 1977 Fragments arranged by size: 2767 1977 1452 578 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2559 2802 3374 2559 Size: 243 572 7172 Fragments arranged by size: 7172 572 243 BtgI C'CryG_G Cuts at: 513 1065 2022 2028 2367 2496 2546 2928 3531 Size: 552 957 6 339 129 50 382 603 Cuts at: 3531 3804 4235 4380 4458 4542 4854 4909 513 Size: 273 431 145 78 84 312 55 3591 Fragments arranged by size: 3591 957 603 552 431 382 339 312 273 145 129 84 78 55 50 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5159 487 Size: 683 56 3933 3315 Fragments arranged by size: 3933 3315 683 56 BtsI GCAGTG_nn' Cuts at: 2393 2983 3160 3299 3313 3569 3635 4333 5030 Size: 590 177 139 14 256 66 698 697 Cuts at: 5030 5503 6297 6317 2393 Size: 473 794 20 4063 Fragments arranged by size: 4063 794 698 697 590 473 256 177 139 66 20 14 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7987 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7952 DraI TTT'AAA Cuts at: 5291 5291 Size: 7987 DraIII CAC_nnn'GTG Cuts at: 2608 2608 Size: 7987 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6994 809 Size: 507 5678 1802 Fragments arranged by size: 5678 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2073 2077 Size: 54 1425 50 275 18 195 48 4 Cuts at: 2077 2364 2499 2592 2994 3713 3909 3966 4277 Size: 287 135 93 402 719 196 57 311 Cuts at: 4277 4286 4446 4603 4878 5434 6323 7952 8 Size: 9 160 157 275 556 889 1629 43 Fragments arranged by size: 1629 1425 889 719 556 402 311 287 275 275 196 195 160 157 135 93 57 54 50 48 43 18 9 4 EagI C'GGCC_G Cuts at: 1537 1812 2073 2592 3713 5434 1537 Size: 275 261 519 1121 1721 4090 Fragments arranged by size: 4090 1721 1121 519 275 261 EarI CTCTTCn'nnn_ Cuts at: 1353 2916 3860 4517 5917 1353 Size: 1563 944 657 1400 3423 Fragments arranged by size: 3423 1563 1400 944 657 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2459 3722 6574 6948 7094 387 Size: 1064 138 870 1263 2852 374 146 1280 Fragments arranged by size: 2852 1280 1263 1064 870 374 146 138 EcoICRI GAG'CTC Cuts at: 4309 4309 Size: 7987 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2701 2936 3016 3583 3923 4505 4520 Size: 393 1069 235 80 567 340 582 15 Cuts at: 4520 4700 4747 6050 6647 7434 7702 1239 Size: 180 47 1303 597 787 268 1524 Fragments arranged by size: 1524 1303 1069 787 597 582 567 393 340 268 235 180 80 47 15 EcoNI CCTnn'n_nnAGG Cuts at: 2201 2201 Size: 7987 EcoO109I rG'GnC_Cy Cuts at: 2278 2524 2563 2677 2707 2868 4780 5110 5223 Size: 246 39 114 30 161 1912 330 113 Cuts at: 5223 5268 2278 Size: 45 4997 Fragments arranged by size: 4997 1912 330 246 161 114 113 45 39 30 EcoRI G'AATT_C Cuts at: 5296 5674 5296 Size: 378 7609 FseI GG_CCGG'CC Cuts at: 2079 2079 Size: 7987 FspI TGC'GCA Cuts at: 6493 6493 Size: 7987 HaeII r_GCGC'y Cuts at: 1678 2086 3259 3693 3768 3908 3984 4004 4475 Size: 408 1173 434 75 140 76 20 471 Cuts at: 4475 4534 7134 1678 Size: 59 2600 2531 Fragments arranged by size: 2600 2531 1173 471 434 408 140 76 75 59 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2876 2908 6628 6660 6702 Size: 32 135 32 1304 32 3720 32 42 Cuts at: 6702 6734 1373 Size: 32 2626 Fragments arranged by size: 3720 2626 1304 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4042 5331 5577 669 Size: 988 281 2104 1289 246 3079 Fragments arranged by size: 3079 2104 1289 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7987 HpaI GTT'AAC Cuts at: 5577 5577 Size: 7987 KasI G'GCGC_C Cuts at: 3764 3980 3764 Size: 216 7771 KpnI G_GTAC'C Cuts at: 5312 5312 Size: 7987 MfeI C'AATT_G Cuts at: 5586 5586 Size: 7987 MluI A'CGCG_T Cuts at: 7863 7863 Size: 7987 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2014 4293 7101 7285 1305 Size: 709 2279 2808 184 2007 Fragments arranged by size: 2808 2279 2007 709 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2366 2501 3911 3968 Size: 54 1425 343 195 339 135 1410 57 Cuts at: 3968 4279 10 Size: 311 3718 Fragments arranged by size: 3718 1425 1410 343 339 311 195 135 57 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 3395 4127 4299 4574 4982 5945 Size: 648 1329 900 732 172 275 408 963 Cuts at: 5945 6304 6463 518 Size: 359 159 2042 Fragments arranged by size: 2042 1329 963 900 732 648 408 359 275 172 159 MspA1I CmG'CkG Cuts at: 1606 2178 2322 2684 2785 3355 3406 3533 3871 Size: 572 144 362 101 570 51 127 338 Cuts at: 3871 3880 4263 4599 4665 4911 5278 5403 6080 Size: 9 383 336 66 246 367 125 677 Cuts at: 6080 7228 7473 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 572 570 383 367 362 338 336 246 245 144 127 125 101 66 51 9 NaeI GCC'GGC Cuts at: 2077 2596 2830 2959 3844 5146 2077 Size: 519 234 129 885 1302 4918 Fragments arranged by size: 4918 1302 885 519 234 129 NarI GG'CG_CC Cuts at: 3765 3981 3765 Size: 216 7771 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6478 Fragments arranged by size: 6478 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7987 NgoMIV G'CCGG_C Cuts at: 2075 2594 2828 2957 3842 5144 2075 Size: 519 234 129 885 1302 4918 Fragments arranged by size: 4918 1302 885 519 234 129 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7987 NotI GC'GGCC_GC Cuts at: 5434 5434 Size: 7987 NspI r_CATG'y Cuts at: 1132 1229 3394 3826 4175 5345 6890 1132 Size: 97 2165 432 349 1170 1545 2229 Fragments arranged by size: 2229 2165 1545 1170 432 349 97 PasI CC'CwG_GG Cuts at: 1348 2703 2848 1348 Size: 1355 145 6487 Fragments arranged by size: 6487 1355 145 PciI A'CATG_T Cuts at: 6886 6886 Size: 7987 PflMI CCAn_nnn'nTGG Cuts at: 1108 4558 4816 5167 1108 Size: 3450 258 351 3928 Fragments arranged by size: 3928 3450 351 258 PfoI T'CCnGG_A Cuts at: 2574 3135 4185 4250 4515 2574 Size: 561 1050 65 265 6046 Fragments arranged by size: 6046 1050 561 265 65 PmeI GTTT'AAAC Cuts at: 5291 5291 Size: 7987 PmlI CAC'GTG Cuts at: 3397 3618 3798 4813 5020 3397 Size: 221 180 1015 207 6364 Fragments arranged by size: 6364 1015 221 207 180 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 6056 6088 7569 7601 4073 Size: 32 1951 32 1481 32 4459 Fragments arranged by size: 4459 1951 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2278 2707 4780 5110 5223 2278 Size: 429 2073 330 113 5042 Fragments arranged by size: 5042 2073 429 330 113 PshAI GACnn'nnGTC Cuts at: 1613 3217 1613 Size: 1604 6383 PsiI TTA'TAA Cuts at: 5557 5557 Size: 7987 PspOMI G'GGCC_C Cuts at: 2524 2677 3509 5267 2524 Size: 153 832 1758 5244 Fragments arranged by size: 5244 1758 832 153 PspXI vC'TCGA_Gb Cuts at: 4831 4831 Size: 7987 PstI C_TGCA'G Cuts at: 830 2742 2790 2837 3660 4008 5339 830 Size: 1912 48 47 823 348 1331 3478 Fragments arranged by size: 3478 1912 1331 823 348 48 47 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7987 PvuII CAG'CTG Cuts at: 2322 2684 2785 3880 4263 4665 2322 Size: 362 101 1095 383 402 5644 Fragments arranged by size: 5644 1095 402 383 362 101 SacI G_AGCT'C Cuts at: 4311 4311 Size: 7987 SacII CC_GC'GG Cuts at: 3534 4912 3534 Size: 1378 6609 SalI G'TCGA_C Cuts at: 1936 5329 1936 Size: 3393 4594 SanDI GG'GwC_CC Cuts at: 2707 4780 5223 2707 Size: 2073 443 5471 Fragments arranged by size: 5471 2073 443 SapI GCTCTTCn'nnn_ Cuts at: 4517 4517 Size: 7987 SbfI CC_TGCA'GG Cuts at: 3660 5339 3660 Size: 1679 6308 ScaI AGT'ACT Cuts at: 6235 7947 6235 Size: 1712 6275 SexAI A'CCwGG_T Cuts at: 3050 3720 3771 3050 Size: 670 51 7266 Fragments arranged by size: 7266 670 51 SfcI C'TryA_G Cuts at: 826 1045 2738 2786 2833 3656 4004 5335 6470 Size: 219 1693 48 47 823 348 1331 1135 Cuts at: 6470 7151 7342 826 Size: 681 191 1471 Fragments arranged by size: 1693 1471 1331 1135 823 681 348 219 191 48 47 SfiI GGCCn_nnn'nGGCC Cuts at: 3233 4285 3233 Size: 1052 6935 SfoI GGC'GCC Cuts at: 3766 3982 3766 Size: 216 7771 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7987 SgrAI Cr'CCGG_yG Cuts at: 1700 2794 3977 1700 Size: 1094 1183 5710 Fragments arranged by size: 5710 1183 1094 SmaI CCC'GGG Cuts at: 1872 2638 3261 3508 5314 1872 Size: 766 623 247 1806 4545 Fragments arranged by size: 4545 1806 766 623 247 SmlI C'TyrA_G Cuts at: 820 1017 1943 4831 6091 6992 7254 820 Size: 197 926 2888 1260 901 262 1553 Fragments arranged by size: 2888 1553 1260 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5284 5778 493 Size: 4791 494 2702 Fragments arranged by size: 4791 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7987 SphI G_CATG'C Cuts at: 1132 3394 4175 5345 1132 Size: 2262 781 1170 3774 Fragments arranged by size: 3774 2262 1170 781 SrfI GCCC'GGGC Cuts at: 3261 3261 Size: 7987 SspI AAT'ATT Cuts at: 5 52 5911 5 Size: 47 5859 2081 Fragments arranged by size: 5859 2081 47 StuI AGG'CCT Cuts at: 1517 2210 3963 4760 5009 5192 1517 Size: 693 1753 797 249 183 4312 Fragments arranged by size: 4312 1753 797 693 249 183 StyI C'CwwG_G Cuts at: 513 1065 2022 2313 3432 5004 5217 5426 513 Size: 552 957 291 1119 1572 213 209 3074 Fragments arranged by size: 3074 1572 1119 957 552 291 213 209 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3173 3874 4088 5174 6014 6031 6184 Size: 1255 1593 701 214 1086 840 17 153 Cuts at: 6184 6369 6814 325 Size: 185 445 1498 Fragments arranged by size: 1593 1498 1255 1086 840 701 445 214 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4824 6233 7945 Size: 274 80 33 51 1443 2847 1409 1712 Cuts at: 7945 96 Size: 138 Fragments arranged by size: 2847 1712 1443 1409 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2283 2315 2616 2648 2991 3023 3782 3814 7947 Size: 32 301 32 343 32 759 32 4133 Cuts at: 7947 7979 2283 Size: 32 2291 Fragments arranged by size: 4133 2291 759 343 301 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4361 1316 Size: 3045 4942 XbaI T'CTAG_A Cuts at: 5323 5323 Size: 7987 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3811 3961 4456 3811 Size: 150 495 7342 Fragments arranged by size: 7342 495 150 XhoI C'TCGA_G Cuts at: 1943 4831 1943 Size: 2888 5099 XmaI C'CCGG_G Cuts at: 1870 2636 3259 3506 5312 1870 Size: 766 623 247 1806 4545 Fragments arranged by size: 4545 1806 766 623 247 XmnI GAAnn'nnTTC Cuts at: 6116 6116 Size: 7987 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4672 5795 276 Size: 53 83 186 936 3138 1123 2468 Fragments arranged by size: 3138 2468 1123 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PsrI RsrII SwaI