Restriction map for
pFN21ASDB0004
(Linear) (Six-base) MAPSORT of: KIBB0004.seq Check: 2774 from: 1 to: 3258
KIBB0004.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2282 2540 3258
Size: 2282 258 718
Fragments arranged by size:
2282 718 258
AatII G_ACGT'C
Cuts at: 0 2651 3258
Size: 2651 607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 1560 3258
Size: 678 882 1698
Fragments arranged by size:
1698 882 678
AfeI AGC'GCT
Cuts at: 0 2450 3258
Size: 2450 808
AgeI A'CCGG_T
Cuts at: 0 771 3258
Size: 771 2487
AleI CACnn'nnGTG
Cuts at: 0 472 2276 3258
Size: 472 1804 982
Fragments arranged by size:
1804 982 472
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2050 2082 3258
Size: 2050 32 1176
Fragments arranged by size:
2050 1176 32
AlwNI CAG_nnn'CTG
Cuts at: 0 485 1172 1184 1320 1545 1641 3090 3182
Size: 485 687 12 136 225 96 1449 92
Cuts at: 3182 3258
Size: 76
Fragments arranged by size:
1449 687 485 225 136 96 92 76
12
ApaI G_GGCC'C
Cuts at: 0 505 658 1490 3248 3258
Size: 505 153 832 1758 10
Fragments arranged by size:
1758 832 505 153 10
ApaLI G'TGCA_C
Cuts at: 0 1044 2374 3258
Size: 1044 1330 884
Fragments arranged by size:
1330 1044 884
AvaI C'yCGr_G
Cuts at: 0 613 831 948 1228 1236 1483 1489 2760
Size: 613 218 117 280 8 247 6 1271
Cuts at: 2760 2808 3258
Size: 48 450
Fragments arranged by size:
1271 613 450 280 247 218 117 48
8 6
BamHI G'GATC_C
Cuts at: 0 527 3258
Size: 527 2731
BanI G'GyrC_C
Cuts at: 0 622 774 916 1741 1957 2077 2185 2270
Size: 622 152 142 825 216 120 108 85
Cuts at: 2270 2719 3258
Size: 449 539
Fragments arranged by size:
825 622 539 449 216 152 142 120
108 85
BanII G_rGCy'C
Cuts at: 0 290 353 428 505 658 822 832 1130
Size: 290 63 75 77 153 164 10 298
Cuts at: 1130 1490 2015 2288 2831 2954 3248 3258
Size: 360 525 273 543 123 294 10
Fragments arranged by size:
543 525 360 298 294 290 273 164
153 123 77 75 63 10 10
BbeI G_GCGC'C
Cuts at: 0 1745 1961 3258
Size: 1745 216 1297
Fragments arranged by size:
1745 1297 216
BbsI GAAGACnn'nnnn_
Cuts at: 0 1566 2307 3258
Size: 1566 741 951
Fragments arranged by size:
1566 951 741
BbvCI CC'TCA_GC
Cuts at: 0 1313 2587 3258
Size: 1313 1274 671
Fragments arranged by size:
1313 1274 671
BglI GCCn_nnn'nGGC
Cuts at: 0 1175 1210 1353 2262 2734 2912 3258
Size: 1175 35 143 909 472 178 346
Fragments arranged by size:
1175 909 472 346 178 143 35
BlpI GC'TnA_GC
Cuts at: 0 121 1987 3258
Size: 121 1866 1271
Fragments arranged by size:
1866 1271 121
Bme1580I G_kGCm'C
Cuts at: 0 505 658 1048 1490 2188 2273 2378 2722
Size: 505 153 390 442 698 85 105 344
Cuts at: 2722 3248 3258
Size: 526 10
Fragments arranged by size:
698 526 505 442 390 344 153 105
85 10
BmgBI CAC'GTC
Cuts at: 0 582 3258
Size: 582 2676
BmrI ACTGGGnnnn_n'
Cuts at: 0 2709 3258
Size: 2709 549
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2487 3258
Size: 2487 771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 993 1900 2482 2497 2677 2724 3258
Size: 913 80 907 582 15 180 47 534
Fragments arranged by size:
913 907 582 534 180 80 47 15
Bpu10I CC'TnA_GC
Cuts at: 0 1313 1663 2587 3258
Size: 1313 350 924 671
Fragments arranged by size:
1313 924 671 350
BsaAI yAC'GTr
Cuts at: 0 1374 1595 1775 2790 2997 3258
Size: 1374 221 180 1015 207 261
Fragments arranged by size:
1374 1015 261 221 207 180
BsaHI Gr'CG_yC
Cuts at: 0 1742 1958 2312 2516 2648 2861 3258
Size: 1742 216 354 204 132 213 397
Fragments arranged by size:
1742 397 354 216 213 204 132
BsaWI w'CCGG_w
Cuts at: 0 771 3258
Size: 771 2487
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 593 623 968 998 2050 2080 2338 2368
Size: 593 30 345 30 1052 30 258 30
Cuts at: 2368 3258
Size: 890
Fragments arranged by size:
1052 890 593 345 258 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 397 400 409 418 421 424 1819
Size: 145 252 3 9 9 3 3 1395
Cuts at: 1819 2023 3258
Size: 204 1235
Fragments arranged by size:
1395 1235 252 204 145 9 9 3
3 3
BseYI C'CCAG_C
Cuts at: 0 437 639 657 758 788 836 841 1165
Size: 437 202 18 101 30 48 5 324
Cuts at: 1165 1328 1644 2062 2416 2840 3104 3258
Size: 163 316 418 354 424 264 154
Fragments arranged by size:
437 424 418 354 324 316 264 202
163 154 101 48 30 18 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 141 445 1087 1543 1754 1879 2422
Size: 18 123 304 642 456 211 125 543
Cuts at: 2422 2977 2994 3019 3061 3258
Size: 555 17 25 42 197
Fragments arranged by size:
642 555 543 456 304 211 197 125
123 42 25 18 17
BsiEI CG_ry'CG
Cuts at: 0 11 53 572 1693 3253 3258
Size: 11 42 519 1121 1560 5
Fragments arranged by size:
1560 1121 519 42 11 5
BsiHKAI G_wGCw'C
Cuts at: 0 70 1048 1081 2288 2378 3258
Size: 70 978 33 1207 90 880
Fragments arranged by size:
1207 978 880 90 70 33
Bsp1286I G_dGCh'C
Cuts at: 0 70 290 353 428 505 658 822 832
Size: 70 220 63 75 77 153 164 10
Cuts at: 832 1048 1081 1130 1490 2015 2188 2273 2288
Size: 216 33 49 360 525 173 85 15
Cuts at: 2288 2378 2722 2831 2954 3248 3258
Size: 90 344 109 123 294 10
Fragments arranged by size:
525 360 344 294 220 216 173 164
153 123 109 90 85 77 75 70
63 49 33 15 10 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 304 2282 2540 2929 2999 3075 3258
Size: 304 1978 258 389 70 76 183
Fragments arranged by size:
1978 389 304 258 183 76 70
BsrBI CCG'CTC
Cuts at: 0 418 1689 1707 2466 2895 3216 3258
Size: 418 1271 18 759 429 321 42
Fragments arranged by size:
1271 759 429 418 321 42 18
BsrFI r'CCGG_y
Cuts at: 0 52 571 771 805 934 1819 1954 3121
Size: 52 519 200 34 129 885 135 1167
Cuts at: 3121 3232 3258
Size: 111 26
Fragments arranged by size:
1167 885 519 200 135 129 111 52
34 26
BsrGI T'GTAC_A
Cuts at: 0 2801 3258
Size: 2801 457
BssHII G'CGCG_C
Cuts at: 0 2429 2619 3258
Size: 2429 190 639
Fragments arranged by size:
2429 639 190
BssSI C'ACGA_G
Cuts at: 0 2899 3258
Size: 2899 359
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1184 1545 1641 2921 3258
Size: 1184 361 96 1280 337
Fragments arranged by size:
1280 1184 361 337 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1120 1788 3258
Size: 1120 668 1470
Fragments arranged by size:
1470 1120 668
BstYI r'GATC_y
Cuts at: 0 527 3258
Size: 527 2731
Bsu36I CC'TnA_GG
Cuts at: 0 536 779 1351 3258
Size: 536 243 572 1907
Fragments arranged by size:
1907 572 536 243
BtgI C'CryG_G
Cuts at: 0 5 344 473 523 905 1508 1781 2212
Size: 5 339 129 50 382 603 273 431
Cuts at: 2212 2357 2435 2519 2831 2886 3258
Size: 145 78 84 312 55 372
Fragments arranged by size:
603 431 382 372 339 312 273 145
129 84 78 55 50 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3136 3258
Size: 3136 122
BtsI GCAGTG_nn'
Cuts at: 0 370 960 1137 1276 1290 1546 1612 2310
Size: 370 590 177 139 14 256 66 698
Cuts at: 2310 3007 3258
Size: 697 251
Fragments arranged by size:
698 697 590 370 256 251 177 139
66 14
DraIII CAC_nnn'GTG
Cuts at: 0 585 3258
Size: 585 2673
EaeI y'GGCC_r
Cuts at: 0 2 50 54 341 476 569 971 1690
Size: 2 48 4 287 135 93 402 719
Cuts at: 1690 1886 1943 2254 2263 2423 2580 2855 3258
Size: 196 57 311 9 160 157 275 403
Fragments arranged by size:
719 403 402 311 287 275 196 160
157 135 93 57 48 9 4 2
EagI C'GGCC_G
Cuts at: 0 50 569 1690 3258
Size: 50 519 1121 1568
Fragments arranged by size:
1568 1121 519 50
EarI CTCTTCn'nnn_
Cuts at: 0 893 1837 2494 3258
Size: 893 944 657 764
Fragments arranged by size:
944 893 764 657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 436 1699 3258
Size: 436 1263 1559
Fragments arranged by size:
1559 1263 436
EcoICRI GAG'CTC
Cuts at: 0 2286 3258
Size: 2286 972
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 913 993 1560 1900 2482 2497 2677
Size: 678 235 80 567 340 582 15 180
Cuts at: 2677 2724 3258
Size: 47 534
Fragments arranged by size:
678 582 567 534 340 235 180 80
47 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 178 3258
Size: 178 3080
EcoO109I rG'GnC_Cy
Cuts at: 0 255 501 540 654 684 845 2757 3087
Size: 255 246 39 114 30 161 1912 330
Cuts at: 3087 3200 3245 3258
Size: 113 45 13
Fragments arranged by size:
1912 330 255 246 161 114 113 45
39 30 13
FseI GG_CCGG'CC
Cuts at: 0 56 3258
Size: 56 3202
HaeII r_GCGC'y
Cuts at: 0 63 1236 1670 1745 1885 1961 1981 2452
Size: 63 1173 434 75 140 76 20 471
Cuts at: 2452 2511 3258
Size: 59 747
Fragments arranged by size:
1173 747 471 434 140 76 75 63
59 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 853 885 3258
Size: 853 32 2373
Fragments arranged by size:
2373 853 32
HincII GTy'rAC
Cuts at: 0 2019 3258
Size: 2019 1239
KasI G'GCGC_C
Cuts at: 0 1741 1957 3258
Size: 1741 216 1301
Fragments arranged by size:
1741 1301 216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2270 3258
Size: 2270 988
MscI TGG'CCA
Cuts at: 0 4 343 478 1888 1945 2256 3258
Size: 4 339 135 1410 57 311 1002
Fragments arranged by size:
1410 1002 339 311 135 57 4
MslI CAynn'nnrTG
Cuts at: 0 472 1372 2104 2276 2551 2959 3258
Size: 472 900 732 172 275 408 299
Fragments arranged by size:
900 732 472 408 299 275 172
MspA1I CmG'CkG
Cuts at: 0 155 299 661 762 1332 1383 1510 1848
Size: 155 144 362 101 570 51 127 338
Cuts at: 1848 1857 2240 2576 2642 2888 3255 3258
Size: 9 383 336 66 246 367 3
Fragments arranged by size:
570 383 367 362 338 336 246 155
144 127 101 66 51 9 3
NaeI GCC'GGC
Cuts at: 0 54 573 807 936 1821 3123 3258
Size: 54 519 234 129 885 1302 135
Fragments arranged by size:
1302 885 519 234 135 129 54
NarI GG'CG_CC
Cuts at: 0 1742 1958 3258
Size: 1742 216 1300
Fragments arranged by size:
1742 1300 216
NgoMIV G'CCGG_C
Cuts at: 0 52 571 805 934 1819 3121 3258
Size: 52 519 234 129 885 1302 137
Fragments arranged by size:
1302 885 519 234 137 129 52
NspI r_CATG'y
Cuts at: 0 1371 1803 2152 3258
Size: 1371 432 349 1106
Fragments arranged by size:
1371 1106 432 349
PasI CC'CwG_GG
Cuts at: 0 680 825 3258
Size: 680 145 2433
Fragments arranged by size:
2433 680 145
PflMI CCAn_nnn'nTGG
Cuts at: 0 2535 2793 3144 3258
Size: 2535 258 351 114
Fragments arranged by size:
2535 351 258 114
PfoI T'CCnGG_A
Cuts at: 0 551 1112 2162 2227 2492 3258
Size: 551 561 1050 65 265 766
Fragments arranged by size:
1050 766 561 551 265 65
PmlI CAC'GTG
Cuts at: 0 1374 1595 1775 2790 2997 3258
Size: 1374 221 180 1015 207 261
Fragments arranged by size:
1374 1015 261 221 207 180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2050 2082 3258
Size: 2050 32 1176
Fragments arranged by size:
2050 1176 32
PpuMI rG'GwC_Cy
Cuts at: 0 255 684 2757 3087 3200 3258
Size: 255 429 2073 330 113 58
Fragments arranged by size:
2073 429 330 255 113 58
PshAI GACnn'nnGTC
Cuts at: 0 1194 3258
Size: 1194 2064
PspOMI G'GGCC_C
Cuts at: 0 501 654 1486 3244 3258
Size: 501 153 832 1758 14
Fragments arranged by size:
1758 832 501 153 14
PspXI vC'TCGA_Gb
Cuts at: 0 2808 3258
Size: 2808 450
PstI C_TGCA'G
Cuts at: 0 719 767 814 1637 1985 3258
Size: 719 48 47 823 348 1273
Fragments arranged by size:
1273 823 719 348 48 47
PvuII CAG'CTG
Cuts at: 0 299 661 762 1857 2240 2642 3258
Size: 299 362 101 1095 383 402 616
Fragments arranged by size:
1095 616 402 383 362 299 101
SacI G_AGCT'C
Cuts at: 0 2288 3258
Size: 2288 970
SacII CC_GC'GG
Cuts at: 0 1511 2889 3258
Size: 1511 1378 369
Fragments arranged by size:
1511 1378 369
SanDI GG'GwC_CC
Cuts at: 0 684 2757 3200 3258
Size: 684 2073 443 58
Fragments arranged by size:
2073 684 443 58
SapI GCTCTTCn'nnn_
Cuts at: 0 2494 3258
Size: 2494 764
SbfI CC_TGCA'GG
Cuts at: 0 1637 3258
Size: 1637 1621
SexAI A'CCwGG_T
Cuts at: 0 1027 1697 1748 3258
Size: 1027 670 51 1510
Fragments arranged by size:
1510 1027 670 51
SfcI C'TryA_G
Cuts at: 0 715 763 810 1633 1981 3258
Size: 715 48 47 823 348 1277
Fragments arranged by size:
1277 823 715 348 48 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1210 2262 3258
Size: 1210 1052 996
Fragments arranged by size:
1210 1052 996
SfoI GGC'GCC
Cuts at: 0 1743 1959 3258
Size: 1743 216 1299
Fragments arranged by size:
1743 1299 216
SgrAI Cr'CCGG_yG
Cuts at: 0 771 1954 3258
Size: 771 1183 1304
Fragments arranged by size:
1304 1183 771
SmaI CCC'GGG
Cuts at: 0 615 1238 1485 3258
Size: 615 623 247 1773
Fragments arranged by size:
1773 623 615 247
SmlI C'TyrA_G
Cuts at: 0 2808 3258
Size: 2808 450
SphI G_CATG'C
Cuts at: 0 1371 2152 3258
Size: 1371 781 1106
Fragments arranged by size:
1371 1106 781
SrfI GCCC'GGGC
Cuts at: 0 1238 3258
Size: 1238 2020
StuI AGG'CCT
Cuts at: 0 187 1940 2737 2986 3169 3258
Size: 187 1753 797 249 183 89
Fragments arranged by size:
1753 797 249 187 183 89
StyI C'CwwG_G
Cuts at: 0 290 1409 2981 3194 3258
Size: 290 1119 1572 213 64
Fragments arranged by size:
1572 1119 290 213 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1150 1851 2065 3151 3258
Size: 1150 701 214 1086 107
Fragments arranged by size:
1150 1086 701 214 107
TatI w'GTAC_w
Cuts at: 0 2801 3258
Size: 2801 457
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 260 292 593 625 968 1000 1759 1791
Size: 260 32 301 32 343 32 759 32
Cuts at: 1791 3258
Size: 1467
Fragments arranged by size:
1467 759 343 301 260 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2338 3258
Size: 2338 920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1788 1938 2433 3258
Size: 1788 150 495 825
Fragments arranged by size:
1788 825 495 150
XhoI C'TCGA_G
Cuts at: 0 2808 3258
Size: 2808 450
XmaI C'CCGG_G
Cuts at: 0 613 1236 1483 3258
Size: 613 623 247 1775
Fragments arranged by size:
1775 623 613 247
ZraI GAC'GTC
Cuts at: 0 2649 3258
Size: 2649 609
Enzymes that do cut:
AarI AatII AcuI AfeI AgeI AleI AloI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FseI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SacII SanDI SapI SbfI SexAI SfcI
SfiI SfoI SgrAI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AhdI AlfI ApoI
AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI
BfrBI BglII BmtI BpuEI BsaI BsaBI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BstBI BstEII BstZ17I ClaI
CspCI DraI DrdI EcoRI EcoRV FalI FspI FspAI
HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PsiI PsrI
PvuI RsrII SalI ScaI SgfI SnaBI SpeI SspI
SwaI XbaI XmnI