(Circular) (Six-base) MAPSORT of: pFN21ASDA2038.seq Check: 7174 from: 1 to: 7351 pFN21ASDA2038 7351 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5161 278 Size: 53 83 186 936 3625 2468 Fragments arranged by size: 3625 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4694 1937 Size: 2757 4594 Acc65I G'GTAC_C Cuts at: 4672 4672 Size: 7351 AclI AA'CG_TT Cuts at: 1563 5478 5851 1563 Size: 3915 373 3063 Fragments arranged by size: 3915 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2314 2380 2716 3933 4278 5414 6798 7066 Size: 682 66 336 1217 345 1136 1384 268 Cuts at: 7066 1632 Size: 1917 Fragments arranged by size: 1917 1384 1217 1136 682 345 336 268 66 AfeI AGC'GCT Cuts at: 2525 3074 3890 2525 Size: 549 816 5986 Fragments arranged by size: 5986 816 549 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7154 AflIII A'CryG_T Cuts at: 1203 6250 7227 1203 Size: 5047 977 1327 Fragments arranged by size: 5047 1327 977 AgeI A'CCGG_T Cuts at: 6182 6182 Size: 7351 AhdI GACnn_n'nnGTC Cuts at: 6080 6080 Size: 7351 AleI CACnn'nnGTG Cuts at: 1166 3101 1166 Size: 1935 5416 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4446 4562 1184 Size: 3262 116 3973 Fragments arranged by size: 3973 3262 116 AlwNI CAG_nnn'CTG Cuts at: 2100 3010 4163 5094 6666 2100 Size: 910 1153 931 1572 2785 Fragments arranged by size: 2785 1572 1153 931 910 ApaI G_GGCC'C Cuts at: 2247 2951 3336 3781 3972 2247 Size: 704 385 445 191 5626 Fragments arranged by size: 5626 704 445 385 191 ApaLI G'TGCA_C Cuts at: 5408 6564 5408 Size: 1156 6195 ApoI r'AATT_y Cuts at: 1493 4660 4886 5038 7147 1493 Size: 3167 226 152 2109 1697 Fragments arranged by size: 3167 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5905 160 Size: 872 4873 1606 Fragments arranged by size: 4873 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7351 AvaI C'yCGr_G Cuts at: 1870 1943 2301 3185 3275 3335 3495 3774 3982 Size: 73 358 884 90 60 160 279 208 Cuts at: 3982 4316 4377 4518 4676 1870 Size: 334 61 141 158 4545 Fragments arranged by size: 4545 884 358 334 279 208 160 158 141 90 73 61 60 BamHI G'GATC_C Cuts at: 4681 5048 4681 Size: 367 6984 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2636 2827 2953 3112 Size: 325 217 627 282 567 191 126 159 Cuts at: 3112 4350 4672 6127 618 Size: 1238 322 1455 1842 Fragments arranged by size: 1842 1455 1238 627 567 325 322 282 217 191 159 126 BanII G_rGCy'C Cuts at: 1391 2247 2475 2951 3126 3192 3257 3282 3336 Size: 856 228 476 175 66 65 25 54 Cuts at: 3336 3366 3781 3910 3972 4122 4188 4491 4670 Size: 30 415 129 62 150 66 303 179 Cuts at: 4670 1391 Size: 4072 Fragments arranged by size: 4072 856 476 415 303 228 179 175 150 129 66 66 65 62 54 30 25 BbeI G_GCGC'C Cuts at: 2073 2640 2957 3116 4354 2073 Size: 567 317 159 1238 5070 Fragments arranged by size: 5070 1238 567 317 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2350 3138 3954 928 Size: 971 451 788 816 4325 Fragments arranged by size: 4325 971 816 788 451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5542 5576 1903 Size: 34 3605 34 3678 Fragments arranged by size: 3678 3605 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5243 6453 5243 Size: 1210 6141 BclI T'GATC_A Cuts at: 1548 1548 Size: 7351 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2045 2836 2946 Size: 107 122 71 631 605 373 791 110 Cuts at: 2946 3361 3776 5962 136 Size: 415 415 2186 1525 Fragments arranged by size: 2186 1525 791 631 605 415 415 373 122 110 107 71 BglII A'GATC_T Cuts at: 4641 7346 4641 Size: 2705 4646 BlpI GC'TnA_GC Cuts at: 4768 4768 Size: 7351 Bme1580I G_kGCm'C Cuts at: 1790 2247 2832 2951 3336 3781 3972 5412 6568 Size: 457 585 119 385 445 191 1440 1156 Cuts at: 6568 1790 Size: 2573 Fragments arranged by size: 2573 1440 1156 585 457 445 385 191 119 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7351 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3271 5082 6040 Size: 416 53 83 399 24 1854 1811 958 Cuts at: 6040 442 Size: 1753 Fragments arranged by size: 1854 1811 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3215 1056 Size: 2159 5192 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4480 4480 Size: 7351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2080 2240 2771 3411 3468 3508 3568 3596 Size: 841 160 531 640 57 40 60 28 Cuts at: 3596 4136 4140 4475 4651 6011 1239 Size: 540 4 335 176 1360 2579 Fragments arranged by size: 2579 1360 841 640 540 531 335 176 160 60 57 40 28 4 Bpu10I CC'TnA_GC Cuts at: 2702 4164 2702 Size: 1462 5889 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5476 6341 6639 5476 Size: 865 298 6188 Fragments arranged by size: 6188 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4468 6014 882 Size: 617 2969 1546 2219 Fragments arranged by size: 2969 2219 1546 617 BsaAI yAC'GTr Cuts at: 493 1204 3250 4648 5142 493 Size: 711 2046 1398 494 2702 Fragments arranged by size: 2702 2046 1398 711 494 BsaBI GATnn'nnATC Cuts at: 2255 7345 2255 Size: 5090 2261 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2151 2637 2954 Size: 53 83 186 936 537 81 486 317 Cuts at: 2954 3113 4351 5158 5540 6175 275 Size: 159 1238 807 382 635 1451 Fragments arranged by size: 1451 1238 936 807 635 537 486 382 317 186 159 83 81 53 BsaWI w'CCGG_w Cuts at: 5784 6182 6456 6603 7014 5784 Size: 398 274 147 411 6121 Fragments arranged by size: 6121 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2486 2516 3041 3071 3077 Size: 30 165 30 1083 30 525 30 6 Cuts at: 3077 3107 3359 3389 3650 3680 1178 Size: 30 252 30 261 30 4849 Fragments arranged by size: 4849 1083 525 261 252 165 30 30 30 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 2357 3326 3473 3592 3849 4054 4069 Size: 1051 120 969 147 119 257 205 15 Cuts at: 4069 4072 4075 4341 4405 4472 1186 Size: 3 3 266 64 67 4065 Fragments arranged by size: 4065 1051 969 266 257 205 147 120 119 67 64 15 3 3 BseYI C'CCAG_C Cuts at: 2554 2596 2831 2923 3578 3727 3761 4024 4138 Size: 42 235 92 655 149 34 263 114 Cuts at: 4138 6554 7050 2554 Size: 2416 496 2855 Fragments arranged by size: 2855 2416 655 496 263 235 149 114 92 42 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2846 2945 4256 4428 4568 1163 Size: 573 1110 99 1311 172 140 3946 Fragments arranged by size: 3946 1311 1110 573 172 140 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 2840 3102 3353 3517 3802 Size: 275 186 39 800 262 251 164 285 Cuts at: 3802 4801 5562 6590 1540 Size: 999 761 1028 2301 Fragments arranged by size: 2301 1028 999 800 761 285 275 262 251 186 164 39 BsiHKAI G_wGCw'C Cuts at: 2475 2778 3126 3910 4333 5412 5497 6568 2475 Size: 303 348 784 423 1079 85 1071 3258 Fragments arranged by size: 3258 1079 1071 784 423 348 303 85 BsmI GAATG_Cn' Cuts at: 4862 4955 4862 Size: 93 7258 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 2475 2778 2832 2951 3126 3192 Size: 399 457 228 303 54 119 175 66 Cuts at: 3192 3257 3282 3336 3366 3781 3910 3972 4122 Size: 65 25 54 30 415 129 62 150 Cuts at: 4122 4188 4333 4491 4670 5412 5497 6568 1391 Size: 66 145 158 179 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 457 415 399 303 228 179 175 158 150 145 129 119 85 66 66 65 62 54 54 30 25 BspEI T'CCGG_A Cuts at: 7014 7014 Size: 7351 BspHI T'CATG_A Cuts at: 5240 6970 5240 Size: 1730 5621 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2868 4588 4706 844 Size: 2024 1720 118 3489 Fragments arranged by size: 3489 2024 1720 118 BsrBI CCG'CTC Cuts at: 1394 1823 2080 3798 5238 1394 Size: 429 257 1718 1440 3507 Fragments arranged by size: 3507 1718 1440 429 257 BsrDI GCAATG_nn' Cuts at: 65 1248 2881 3511 5846 6020 65 Size: 1183 1633 630 2335 174 1396 Fragments arranged by size: 2335 1633 1396 1183 630 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 2187 2644 2812 2835 3217 3227 Size: 110 238 139 457 168 23 382 10 Cuts at: 3227 3516 3565 5995 6182 1700 Size: 289 49 2430 187 2869 Fragments arranged by size: 2869 2430 457 382 289 238 187 168 139 110 49 23 10 BsrGI T'GTAC_A Cuts at: 96 2442 96 Size: 2346 5005 BssHII G'CGCG_C Cuts at: 1925 2785 3153 3155 3178 1925 Size: 860 368 2 23 6098 Fragments arranged by size: 6098 860 368 23 2 BssSI C'ACGA_G Cuts at: 5411 6423 5411 Size: 1012 6339 BstAPI GCAn_nnn'nTGC Cuts at: 794 2782 2869 2968 3010 794 Size: 1988 87 99 42 5135 Fragments arranged by size: 5135 1988 99 87 42 BstBI TT'CG_AA Cuts at: 2007 6155 2007 Size: 4148 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7351 BstXI CCAn_nnnn'nTGG Cuts at: 2917 3690 4195 2917 Size: 773 505 6073 Fragments arranged by size: 6073 773 505 BstYI r'GATC_y Cuts at: 1972 2496 3300 4641 4681 5048 5433 5450 6902 Size: 524 804 1341 40 367 385 17 1452 Cuts at: 6902 6988 7000 7346 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1341 804 524 385 367 346 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 3940 3940 Size: 7351 BtgI C'CryG_G Cuts at: 513 1065 2022 2613 3096 3387 513 Size: 552 957 591 483 291 4477 Fragments arranged by size: 4477 957 591 552 483 291 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3636 487 Size: 683 56 2410 4202 Fragments arranged by size: 4202 2410 683 56 BtsI GCAGTG_nn' Cuts at: 2198 3640 3646 4200 4867 5661 5681 2198 Size: 1442 6 554 667 794 20 3868 Fragments arranged by size: 3868 1442 794 667 554 20 6 ClaI AT'CG_AT Cuts at: 1554 4530 1554 Size: 2976 4375 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4378 4413 529 Size: 35 3814 35 3467 Fragments arranged by size: 3814 3467 35 35 DraI TTT'AAA Cuts at: 4655 4655 Size: 7351 DraIII CAC_nnn'GTG Cuts at: 2782 2869 2782 Size: 87 7264 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2090 3029 3399 6358 809 Size: 507 774 939 370 2959 1802 Fragments arranged by size: 2959 1802 939 774 507 370 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2046 2349 Size: 54 1425 50 275 18 207 9 303 Cuts at: 2349 2574 2837 3015 3099 3350 3514 4798 5687 Size: 225 263 178 84 251 164 1284 889 Cuts at: 5687 7316 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1284 889 303 275 263 251 225 207 178 164 84 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2037 2837 3099 3350 3514 4798 1537 Size: 275 225 800 262 251 164 1284 4090 Fragments arranged by size: 4090 1284 800 275 262 251 225 164 EarI CTCTTCn'nnn_ Cuts at: 1353 4093 5281 1353 Size: 2740 1188 3423 Fragments arranged by size: 3423 2740 1188 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2507 5938 6312 6458 387 Size: 1064 138 918 3431 374 146 1280 Fragments arranged by size: 3431 1280 1064 918 374 146 138 EcoICRI GAG'CTC Cuts at: 2473 3124 3908 2473 Size: 651 784 5916 Fragments arranged by size: 5916 784 651 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2240 2314 2380 2716 2771 3411 Size: 393 448 160 74 66 336 55 640 Cuts at: 3411 3468 3508 3568 3596 3933 4136 4140 4278 Size: 57 40 60 28 337 203 4 138 Cuts at: 4278 4475 4651 5414 6011 6798 7066 1239 Size: 197 176 763 597 787 268 1524 Fragments arranged by size: 1524 787 763 640 597 448 393 337 336 268 203 197 176 160 138 74 66 60 57 55 40 28 4 EcoNI CCTnn'n_nnAGG Cuts at: 2222 2294 4457 2222 Size: 72 2163 5116 Fragments arranged by size: 5116 2163 72 EcoO109I rG'GnC_Cy Cuts at: 2947 3007 3022 3332 3340 3778 3944 3986 4279 Size: 60 15 310 8 438 166 42 293 Cuts at: 4279 4313 4361 2947 Size: 34 48 5937 Fragments arranged by size: 5937 438 310 293 166 60 48 42 34 15 8 EcoRI G'AATT_C Cuts at: 4660 5038 4660 Size: 378 6973 FseI GG_CCGG'CC Cuts at: 2052 2052 Size: 7351 FspI TGC'GCA Cuts at: 5857 5857 Size: 7351 HaeII r_GCGC'y Cuts at: 1678 2073 2527 2573 2640 2936 2957 3040 3057 Size: 395 454 46 67 296 21 83 17 Cuts at: 3057 3076 3116 3218 3419 3892 4354 6498 1678 Size: 19 40 102 201 473 462 2144 2531 Fragments arranged by size: 2531 2144 473 462 454 395 296 201 102 83 67 46 40 21 19 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3041 3073 3077 3109 3359 Size: 32 135 32 1469 32 4 32 250 Cuts at: 3359 3391 3650 3682 4400 4432 4512 4544 5992 Size: 32 259 32 718 32 80 32 1448 Cuts at: 5992 6024 6066 6098 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1469 1448 718 259 250 135 80 42 32 32 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 4695 4941 669 Size: 988 281 2757 246 3079 Fragments arranged by size: 3079 2757 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7351 HpaI GTT'AAC Cuts at: 4941 4941 Size: 7351 KasI G'GCGC_C Cuts at: 2069 2636 2953 3112 4350 2069 Size: 567 317 159 1238 5070 Fragments arranged by size: 5070 1238 567 317 159 KpnI G_GTAC'C Cuts at: 4676 4676 Size: 7351 MfeI C'AATT_G Cuts at: 4950 4950 Size: 7351 MluI A'CGCG_T Cuts at: 7227 7227 Size: 7351 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6465 6649 1305 Size: 5160 184 2007 Fragments arranged by size: 5160 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2351 10 Size: 54 1425 343 519 5010 Fragments arranged by size: 5010 1425 519 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2375 2480 2675 3101 4544 5309 5668 Size: 648 1209 105 195 426 1443 765 359 Cuts at: 5668 5827 518 Size: 159 2042 Fragments arranged by size: 2042 1443 1209 765 648 426 359 195 159 105 MspA1I CmG'CkG Cuts at: 1606 2100 2615 2749 2959 3119 3389 3513 3852 Size: 494 515 134 210 160 270 124 339 Cuts at: 3852 3953 4052 4301 4767 5444 6592 6837 1606 Size: 101 99 249 466 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 515 494 466 339 270 249 245 210 160 134 124 101 99 NaeI GCC'GGC Cuts at: 2050 2189 2837 3219 3229 3518 2050 Size: 139 648 382 10 289 5883 Fragments arranged by size: 5883 648 382 289 139 10 NarI GG'CG_CC Cuts at: 2070 2637 2954 3113 4351 2070 Size: 567 317 159 1238 5070 Fragments arranged by size: 5070 1238 567 317 159 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5842 Fragments arranged by size: 5842 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7351 NgoMIV G'CCGG_C Cuts at: 2048 2187 2835 3217 3227 3516 2048 Size: 139 648 382 10 289 5883 Fragments arranged by size: 5883 648 382 289 139 10 NheI G'CTAG_C Cuts at: 1052 3211 1052 Size: 2159 5192 NotI GC'GGCC_GC Cuts at: 4798 4798 Size: 7351 NspI r_CATG'y Cuts at: 1132 1229 2462 4709 6254 1132 Size: 97 1233 2247 1545 2229 Fragments arranged by size: 2247 2229 1545 1233 97 PasI CC'CwG_GG Cuts at: 1348 2273 3359 1348 Size: 925 1086 5340 Fragments arranged by size: 5340 1086 925 PciI A'CATG_T Cuts at: 6250 6250 Size: 7351 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7351 PfoI T'CCnGG_A Cuts at: 2289 3612 4113 2289 Size: 1323 501 5527 Fragments arranged by size: 5527 1323 501 PmeI GTTT'AAAC Cuts at: 4655 4655 Size: 7351 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5420 5452 6933 6965 5420 Size: 32 1481 32 5806 Fragments arranged by size: 5806 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3022 3986 4279 4313 3022 Size: 964 293 34 6060 Fragments arranged by size: 6060 964 293 34 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7351 PsiI TTA'TAA Cuts at: 4921 4921 Size: 7351 PspOMI G'GGCC_C Cuts at: 2243 2947 3332 3777 3968 2243 Size: 704 385 445 191 5626 Fragments arranged by size: 5626 704 445 385 191 PstI C_TGCA'G Cuts at: 830 2268 2760 3564 4311 4599 4703 830 Size: 1438 492 804 747 288 104 3478 Fragments arranged by size: 3478 1438 804 747 492 288 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7351 PvuII CAG'CTG Cuts at: 3852 4052 4301 3852 Size: 200 249 6902 Fragments arranged by size: 6902 249 200 SacI G_AGCT'C Cuts at: 2475 3126 3910 2475 Size: 651 784 5916 Fragments arranged by size: 5916 784 651 SacII CC_GC'GG Cuts at: 2616 3390 2616 Size: 774 6577 SalI G'TCGA_C Cuts at: 1936 4693 1936 Size: 2757 4594 SanDI GG'GwC_CC Cuts at: 3022 4313 3022 Size: 1291 6060 SbfI CC_TGCA'GG Cuts at: 4703 4703 Size: 7351 ScaI AGT'ACT Cuts at: 3092 5599 7311 3092 Size: 2507 1712 3132 Fragments arranged by size: 3132 2507 1712 SexAI A'CCwGG_T Cuts at: 4275 4275 Size: 7351 SfcI C'TryA_G Cuts at: 826 1045 2264 2756 3560 4307 4595 4699 5834 Size: 219 1219 492 804 747 288 104 1135 Cuts at: 5834 6515 6706 826 Size: 681 191 1471 Fragments arranged by size: 1471 1219 1135 804 747 681 492 288 219 191 104 SfiI GGCCn_nnn'nGGCC Cuts at: 2045 3776 2045 Size: 1731 5620 SfoI GGC'GCC Cuts at: 2071 2638 2955 3114 4352 2071 Size: 567 317 159 1238 5070 Fragments arranged by size: 5070 1238 567 317 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7351 SgrAI Cr'CCGG_yG Cuts at: 1700 3227 1700 Size: 1527 5824 SmaI CCC'GGG Cuts at: 1872 2303 3187 3497 3776 4379 4678 1872 Size: 431 884 310 279 603 299 4545 Fragments arranged by size: 4545 884 603 431 310 299 279 SmlI C'TyrA_G Cuts at: 820 1017 1943 5455 6356 6618 820 Size: 197 926 3512 901 262 1553 Fragments arranged by size: 3512 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4648 5142 493 Size: 4155 494 2702 Fragments arranged by size: 4155 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7351 SphI G_CATG'C Cuts at: 1132 2462 4709 1132 Size: 1330 2247 3774 Fragments arranged by size: 3774 2247 1330 SspI AAT'ATT Cuts at: 5 52 5275 5 Size: 47 5223 2081 Fragments arranged by size: 5223 2081 47 StuI AGG'CCT Cuts at: 1517 3770 4043 4247 1517 Size: 2253 273 204 4621 Fragments arranged by size: 4621 2253 273 204 StyI C'CwwG_G Cuts at: 513 1065 2022 2125 2517 4242 4345 4790 513 Size: 552 957 103 392 1725 103 445 3074 Fragments arranged by size: 3074 1725 957 552 445 392 103 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5378 5395 5548 5733 6178 325 Size: 1255 3798 17 153 185 445 1498 Fragments arranged by size: 3798 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2442 3090 5597 Size: 274 80 33 51 1443 465 648 2507 Cuts at: 5597 7309 96 Size: 1712 138 Fragments arranged by size: 2507 1712 1443 648 465 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2486 2518 3970 4002 7311 7343 2486 Size: 32 1452 32 3309 32 2494 Fragments arranged by size: 3309 2494 1452 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7351 XbaI T'CTAG_A Cuts at: 4687 4687 Size: 7351 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2029 Size: 7351 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7351 XmaI C'CCGG_G Cuts at: 1870 2301 3185 3495 3774 4377 4676 1870 Size: 431 884 310 279 603 299 4545 Fragments arranged by size: 4545 884 603 431 310 299 279 XmnI GAAnn'nnTTC Cuts at: 5480 5480 Size: 7351 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5159 276 Size: 53 83 186 936 3625 2468 Fragments arranged by size: 3625 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SrfI SwaI