(Circular) (Six-base) MAPSORT of: pFN21ASDA2005.seq Check: 2314 from: 1 to: 9481 pFN21ASDA2005 9481 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7291 278 Size: 53 83 186 936 5755 2468 Fragments arranged by size: 5755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4455 5248 5400 5867 6824 1937 Size: 2518 793 152 467 957 4594 Fragments arranged by size: 4594 2518 957 793 467 152 Acc65I G'GTAC_C Cuts at: 4262 6802 4262 Size: 2540 6941 AclI AA'CG_TT Cuts at: 1563 2230 7608 7981 1563 Size: 667 5378 373 3063 Fragments arranged by size: 5378 3063 667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3183 3573 3660 3930 4125 4291 4661 5639 Size: 1551 390 87 270 195 166 370 978 Cuts at: 5639 6382 6630 7544 8928 9196 1632 Size: 743 248 914 1384 268 1917 Fragments arranged by size: 1917 1551 1384 978 914 743 390 370 270 268 248 195 166 87 AflII C'TTAA_G Cuts at: 820 1017 2102 820 Size: 197 1085 8199 Fragments arranged by size: 8199 1085 197 AflIII A'CryG_T Cuts at: 1203 3459 5592 8380 9357 1203 Size: 2256 2133 2788 977 1327 Fragments arranged by size: 2788 2256 2133 1327 977 AgeI A'CCGG_T Cuts at: 8312 8312 Size: 9481 AhdI GACnn_n'nnGTC Cuts at: 8210 8210 Size: 9481 AleI CACnn'nnGTG Cuts at: 1166 2651 4973 1166 Size: 1485 2322 5674 Fragments arranged by size: 5674 2322 1485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9481 AlwNI CAG_nnn'CTG Cuts at: 7224 8796 7224 Size: 1572 7909 ApaLI G'TGCA_C Cuts at: 7538 8694 7538 Size: 1156 8325 ApoI r'AATT_y Cuts at: 1493 2127 2359 2669 2710 3000 3127 3647 4046 Size: 634 232 310 41 290 127 520 399 Cuts at: 4046 5077 5111 5368 5797 6166 6532 6790 7016 Size: 1031 34 257 429 369 366 258 226 Cuts at: 7016 7168 9277 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1031 634 520 429 399 369 366 310 290 258 257 232 226 152 127 41 34 AseI AT'TA_AT Cuts at: 160 1032 2109 5195 8035 160 Size: 872 1077 3086 2840 1606 Fragments arranged by size: 3086 2840 1606 1077 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9481 AvaI C'yCGr_G Cuts at: 1870 1943 6806 1870 Size: 73 4863 4545 Fragments arranged by size: 4863 4545 73 AvrII C'CTAG_G Cuts at: 6727 6727 Size: 9481 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4957 4990 4957 Size: 33 9448 BamHI G'GATC_C Cuts at: 5953 6811 7178 5953 Size: 858 367 8256 Fragments arranged by size: 8256 858 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2726 4262 4631 6802 8257 Size: 325 217 627 939 1536 369 2171 1455 Cuts at: 8257 618 Size: 1842 Fragments arranged by size: 2171 1842 1536 1455 939 627 369 325 217 BanII G_rGCy'C Cuts at: 1391 6800 1391 Size: 5409 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2103 2677 3450 4911 928 Size: 971 204 574 773 1461 5498 Fragments arranged by size: 5498 1461 971 773 574 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7672 7706 1903 Size: 34 5735 34 3678 Fragments arranged by size: 5735 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4111 7373 8583 4111 Size: 3262 1210 5009 Fragments arranged by size: 5009 3262 1210 BclI T'GATC_A Cuts at: 1548 2527 2823 3375 4365 4692 1548 Size: 979 296 552 990 327 6337 Fragments arranged by size: 6337 990 979 552 327 296 BfrBI ATG'CAT Cuts at: 4279 5267 4279 Size: 988 8493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8092 136 Size: 107 122 71 631 605 6420 1525 Fragments arranged by size: 6420 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3796 5809 9476 3796 Size: 2013 3667 3801 Fragments arranged by size: 3801 3667 2013 BlpI GC'TnA_GC Cuts at: 6898 6898 Size: 9481 Bme1580I G_kGCm'C Cuts at: 1790 5341 7542 8698 1790 Size: 3551 2201 1156 2573 Fragments arranged by size: 3551 2573 2201 1156 BmgBI CAC'GTC Cuts at: 1318 5342 1318 Size: 4024 5457 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5344 7212 8170 Size: 416 53 83 399 24 3927 1868 958 Cuts at: 8170 442 Size: 1753 Fragments arranged by size: 3927 1868 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3066 1056 Size: 2010 7471 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3716 Size: 9481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4073 5975 8141 1239 Size: 2834 1902 2166 2579 Fragments arranged by size: 2834 2579 2166 1902 Bpu10I CC'TnA_GC Cuts at: 5284 5284 Size: 9481 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5254 5485 7606 8471 8769 5254 Size: 231 2121 865 298 5966 Fragments arranged by size: 5966 2121 865 298 231 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 8144 882 Size: 617 6645 2219 Fragments arranged by size: 6645 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6778 7272 493 Size: 711 5574 494 2702 Fragments arranged by size: 5574 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2385 2695 9475 2385 Size: 310 6780 2391 Fragments arranged by size: 6780 2391 310 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7288 7670 8305 275 Size: 53 83 186 936 5755 382 635 1451 Fragments arranged by size: 5755 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7914 8312 8586 8733 9144 7914 Size: 398 274 147 411 8251 Fragments arranged by size: 8251 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2754 2784 2870 2900 5722 Size: 30 165 30 1351 30 86 30 2822 Cuts at: 5722 5752 1178 Size: 30 4907 Fragments arranged by size: 4907 2822 1351 165 86 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 6011 1186 Size: 4825 4656 BseYI C'CCAG_C Cuts at: 3500 4194 8684 9180 3500 Size: 694 4490 496 3801 Fragments arranged by size: 4490 3801 694 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3969 5259 6442 1163 Size: 573 2233 1290 1183 4202 Fragments arranged by size: 4202 2233 1290 1183 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6931 7692 8720 1540 Size: 275 186 4930 761 1028 2301 Fragments arranged by size: 4930 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2590 7542 7627 8698 2590 Size: 4952 85 1071 3373 Fragments arranged by size: 4952 3373 1071 85 BsmI GAATG_Cn' Cuts at: 5267 6992 7085 5267 Size: 1725 93 7663 Fragments arranged by size: 7663 1725 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5575 5575 Size: 9481 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2590 5341 6800 7542 7627 8698 1391 Size: 399 800 2751 1459 742 85 1071 2174 Fragments arranged by size: 2751 2174 1459 1071 800 742 399 85 BspEI T'CCGG_A Cuts at: 9144 9144 Size: 9481 BspHI T'CATG_A Cuts at: 2275 4689 4695 7370 9100 2275 Size: 2414 6 2675 1730 2656 Fragments arranged by size: 2675 2656 2414 1730 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6414 6836 844 Size: 5570 422 3489 Fragments arranged by size: 5570 3489 422 BsrBI CCG'CTC Cuts at: 1394 1823 7368 1394 Size: 429 5545 3507 Fragments arranged by size: 5545 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2683 3192 7976 8150 65 Size: 1183 1435 509 4784 174 1396 Fragments arranged by size: 4784 1435 1396 1183 509 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8125 8312 1700 Size: 110 6315 187 2869 Fragments arranged by size: 6315 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4013 96 Size: 3917 5564 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9481 BssSI C'ACGA_G Cuts at: 7541 8553 7541 Size: 1012 8469 BstAPI GCAn_nnn'nTGC Cuts at: 794 2131 4352 794 Size: 1337 2221 5923 Fragments arranged by size: 5923 2221 1337 BstBI TT'CG_AA Cuts at: 2007 8285 2007 Size: 6278 3203 BstEII G'GTnAC_C Cuts at: 1184 4375 5562 1184 Size: 3191 1187 5103 Fragments arranged by size: 5103 3191 1187 BstXI CCAn_nnnn'nTGG Cuts at: 2653 3570 2653 Size: 917 8564 BstYI r'GATC_y Cuts at: 1972 3694 3796 3938 4544 5809 5953 6392 6811 Size: 1722 102 142 606 1265 144 439 419 Cuts at: 6811 7178 7563 7580 9032 9118 9130 9476 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1722 1452 1265 606 439 419 385 367 346 144 142 102 86 17 12 BstZ17I GTA'TAC Cuts at: 5868 5868 Size: 9481 BtgI C'CryG_G Cuts at: 513 1065 2204 2646 2758 3934 6090 513 Size: 552 1139 442 112 1176 2156 3904 Fragments arranged by size: 3904 2156 1176 1139 552 442 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2531 487 Size: 683 56 1305 7437 Fragments arranged by size: 7437 1305 683 56 BtsI GCAGTG_nn' Cuts at: 4189 6997 7791 7811 4189 Size: 2808 794 20 5859 Fragments arranged by size: 5859 2808 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9481 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2871 2906 529 Size: 35 2307 35 7104 Fragments arranged by size: 7104 2307 35 35 DraI TTT'AAA Cuts at: 3132 3327 3858 6785 3132 Size: 195 531 2927 5828 Fragments arranged by size: 5828 2927 531 195 DraIII CAC_nnn'GTG Cuts at: 4847 4847 Size: 9481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2930 8488 809 Size: 507 1614 5558 1802 Fragments arranged by size: 5558 1802 1614 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3081 6177 6326 Size: 54 1425 50 275 18 1251 3096 149 Cuts at: 6326 6928 7817 9446 8 Size: 602 889 1629 43 Fragments arranged by size: 3096 1629 1425 1251 889 602 275 149 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6928 1537 Size: 275 5116 4090 Fragments arranged by size: 5116 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3162 3679 5067 5098 5139 7411 1353 Size: 1809 517 1388 31 41 2272 3423 Fragments arranged by size: 3423 2272 1809 1388 517 41 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8068 8442 8588 387 Size: 1064 138 6479 374 146 1280 Fragments arranged by size: 6479 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3183 3573 3660 3930 4073 4125 4291 Size: 393 1551 390 87 270 143 52 166 Cuts at: 4291 4661 5639 5975 6382 6630 7544 8141 8928 Size: 370 978 336 407 248 914 597 787 Cuts at: 8928 9196 1239 Size: 268 1524 Fragments arranged by size: 1551 1524 978 914 787 597 407 393 390 370 336 270 268 248 166 143 87 52 EcoNI CCTnn'n_nnAGG Cuts at: 2580 2580 Size: 9481 EcoO109I rG'GnC_Cy Cuts at: 2182 6296 6746 2182 Size: 4114 450 4917 Fragments arranged by size: 4917 4114 450 EcoRI G'AATT_C Cuts at: 2710 6790 7168 2710 Size: 4080 378 5023 Fragments arranged by size: 5023 4080 378 EcoRV GAT'ATC Cuts at: 2407 3076 2407 Size: 669 8812 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2815 4321 5651 6735 2815 Size: 1506 1330 1084 5561 Fragments arranged by size: 5561 1506 1330 1084 FspI TGC'GCA Cuts at: 7987 7987 Size: 9481 HaeII r_GCGC'y Cuts at: 1678 8628 1678 Size: 6950 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3353 3385 3463 3495 5546 Size: 32 135 32 1781 32 78 32 2051 Cuts at: 5546 5578 5722 5754 8122 8154 8196 8228 1373 Size: 32 144 32 2368 32 42 32 2626 Fragments arranged by size: 2626 2368 2051 1781 144 135 78 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4765 6825 7071 669 Size: 988 281 2827 2060 246 3079 Fragments arranged by size: 3079 2827 2060 988 281 246 HindIII A'AGCT_T Cuts at: 748 3393 4172 4897 5202 748 Size: 2645 779 725 305 5027 Fragments arranged by size: 5027 2645 779 725 305 HpaI GTT'AAC Cuts at: 7071 7071 Size: 9481 KpnI G_GTAC'C Cuts at: 4266 6806 4266 Size: 2540 6941 MfeI C'AATT_G Cuts at: 2501 7080 2501 Size: 4579 4902 MluI A'CGCG_T Cuts at: 9357 9357 Size: 9481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3333 5406 8595 8779 1305 Size: 2028 2073 3189 184 2007 Fragments arranged by size: 3189 2073 2028 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3083 6179 6328 10 Size: 54 1425 343 1251 3096 149 3163 Fragments arranged by size: 3163 3096 1425 1251 343 149 54 MslI CAynn'nnrTG Cuts at: 518 1166 2651 2663 2814 2993 3568 4412 4694 Size: 648 1485 12 151 179 575 844 282 Cuts at: 4694 4973 7439 7798 7957 518 Size: 279 2466 359 159 2042 Fragments arranged by size: 2466 2042 1485 844 648 575 359 282 279 179 159 151 12 MspA1I CmG'CkG Cuts at: 1606 3504 6897 7574 8722 8967 1606 Size: 1898 3393 677 1148 245 2120 Fragments arranged by size: 3393 2120 1898 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2204 2758 6090 513 Size: 552 1139 554 3332 3904 Fragments arranged by size: 3904 3332 1139 554 552 NdeI CA'TA_TG Cuts at: 387 5703 6757 387 Size: 5316 1054 3111 Fragments arranged by size: 5316 3111 1054 NheI G'CTAG_C Cuts at: 1052 3062 1052 Size: 2010 7471 NotI GC'GGCC_GC Cuts at: 6928 6928 Size: 9481 NsiI A_TGCA'T Cuts at: 4281 5269 4281 Size: 988 8493 NspI r_CATG'y Cuts at: 1132 1229 2078 3463 4283 5596 6421 6839 8384 Size: 97 849 1385 820 1313 825 418 1545 Cuts at: 8384 1132 Size: 2229 Fragments arranged by size: 2229 1545 1385 1313 849 825 820 418 97 PasI CC'CwG_GG Cuts at: 1348 4884 1348 Size: 3536 5945 PciI A'CATG_T Cuts at: 3459 5592 8380 3459 Size: 2133 2788 4560 Fragments arranged by size: 4560 2788 2133 PflMI CCAn_nnn'nTGG Cuts at: 1108 4256 1108 Size: 3148 6333 PfoI T'CCnGG_A Cuts at: 2281 4050 4547 5991 2281 Size: 1769 497 1444 5771 Fragments arranged by size: 5771 1769 1444 497 PmeI GTTT'AAAC Cuts at: 6785 6785 Size: 9481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7550 7582 9063 9095 7550 Size: 32 1481 32 7936 Fragments arranged by size: 7936 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2182 6296 2182 Size: 4114 5367 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9481 PsiI TTA'TAA Cuts at: 7051 7051 Size: 9481 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4909 4941 4909 Size: 32 9449 PstI C_TGCA'G Cuts at: 830 2166 2907 6129 6833 830 Size: 1336 741 3222 704 3478 Fragments arranged by size: 3478 3222 1336 741 704 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9481 PvuII CAG'CTG Cuts at: 3504 3504 Size: 9481 SalI G'TCGA_C Cuts at: 1936 6823 1936 Size: 4887 4594 SbfI CC_TGCA'GG Cuts at: 2166 6833 2166 Size: 4667 4814 ScaI AGT'ACT Cuts at: 6509 7729 9441 6509 Size: 1220 1712 6549 Fragments arranged by size: 6549 1712 1220 SfcI C'TryA_G Cuts at: 826 1045 2162 2903 4381 5443 6125 6627 6829 Size: 219 1117 741 1478 1062 682 502 202 Cuts at: 6829 7964 8645 8836 826 Size: 1135 681 191 1471 Fragments arranged by size: 1478 1471 1135 1117 1062 741 682 681 502 219 202 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9481 SmaI CCC'GGG Cuts at: 1872 6808 1872 Size: 4936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2102 5233 5500 7585 8486 8748 Size: 197 926 159 3131 267 2085 901 262 Cuts at: 8748 820 Size: 1553 Fragments arranged by size: 3131 2085 1553 926 901 267 262 197 159 SnaBI TAC'GTA Cuts at: 493 6778 7272 493 Size: 6285 494 2702 Fragments arranged by size: 6285 2702 494 SpeI A'CTAG_T Cuts at: 152 4874 152 Size: 4722 4759 SphI G_CATG'C Cuts at: 1132 6839 1132 Size: 5707 3774 SspI AAT'ATT Cuts at: 5 52 2377 4218 4854 7405 5 Size: 47 2325 1841 636 2551 2081 Fragments arranged by size: 2551 2325 2081 1841 636 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9481 StyI C'CwwG_G Cuts at: 513 1065 2204 2758 2882 4611 6090 6727 6920 Size: 552 1139 554 124 1729 1479 637 193 Cuts at: 6920 513 Size: 3074 Fragments arranged by size: 3074 1729 1479 1139 637 554 552 193 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3897 5606 7508 7525 7678 7863 8308 Size: 1255 2317 1709 1902 17 153 185 445 Cuts at: 8308 325 Size: 1498 Fragments arranged by size: 2317 1902 1709 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4013 4877 5004 Size: 274 80 33 51 1443 2036 864 127 Cuts at: 5004 6408 6507 7727 9439 96 Size: 1404 99 1220 1712 138 Fragments arranged by size: 2036 1712 1443 1404 1220 864 274 138 127 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3833 3865 3978 4010 9441 9473 3833 Size: 32 113 32 5431 32 3841 Fragments arranged by size: 5431 3841 113 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3907 6279 1316 Size: 2591 2372 4518 Fragments arranged by size: 4518 2591 2372 XbaI T'CTAG_A Cuts at: 3149 3556 6817 3149 Size: 407 3261 5813 Fragments arranged by size: 5813 3261 407 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5701 5701 Size: 9481 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9481 XmaI C'CCGG_G Cuts at: 1870 6806 1870 Size: 4936 4545 XmnI GAAnn'nnTTC Cuts at: 2424 2898 4304 4921 5081 7610 2424 Size: 474 1406 617 160 2529 4295 Fragments arranged by size: 4295 2529 1406 617 474 160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7289 276 Size: 53 83 186 936 5755 2468 Fragments arranged by size: 5755 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BsiWI Bsu36I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI